Immediate and residual effects of heat stress and restricted intake on milk protein and casein composition and energy metabolism.

The effects of heat stress on dairy production can be separated into 2 distinct causes: those effects that are mediated by the reduced voluntary feed intake associated with heat stress, and the direct physiological and metabolic effects of heat stress. To distinguish between these, and identify their effect on milk protein and casein concentration, mid-lactation Holstein-Friesian cows (n = 24) were housed in temperature-controlled chambers and either subjected to heat stress [HS; temperature-humidity index (THI) ~78] or kept in a THI<70 environment and pair-fed with heat-stressed cows (TN-R) for 7 d. A control group of cows was kept in a THI<70 environment with ad libitum feeding (TN-AL). A subsequent recovery period (7 d), with THI<70 and ad libitum feeding followed. Intake accounted for only part of the effects of heat stress. Heat stress reduced the milk protein concentration, casein number, and casein concentration and increased the urea concentration in milk beyond the effects of restriction of intake. Under HS, the proportion in total casein of αS1-casein increased and the proportion of αS2-casein decreased. Because no effect of HS on milk fat or lactose concentration was found, these effects appeared to be the result of specific downregulation of mammary protein synthesis, and not a general reduction in mammary activity. No residual effects were found of HS or TN-R on milk production or composition after THI<70 and ad libitum intake were restored. Heat-stressed cows had elevated blood concentrations of urea and Ca, compared with TN-R and TN-AL. Cows in TN-R had higher serum nonesterified fatty acid concentrations than cows in HS. It was proposed that HS and TN-R cows may mobilize different tissues as endogenous sources of energy.

Differential voluntary feed intake and whole transcriptome profiling in the hypothalamus of young sheep offered CP and phosphorus-deficient diets.

A reduction in voluntary feed intake is observed in ruminants consuming nutrient-deficient diets, such as those with a low CP or P content, and has been attributed to active metabolic regulation, rather than a physical constraint. The hypothalamus is the key integrator of feed intake regulation in mammals. The objectives of this experiment were to (1) establish a model of metabolic feed intake regulation in ruminants consuming diets of variable CP and P content, and (2) determine key biochemical pathways and influential points of regulation within the hypothalamus. Merino wethers [n = 40; 23.7 ± 1.4 kg liveweight (mean ± SD)] were fed one of five dietary treatments (n = 8/treatment) for 63 days in individual pens. The treatments included targeted combinations of high (H) and low (L) CP (110 and 55 g/kg DM) and high and low P (2.5 and 0.7 g/kg DM) with 9 MJ metabolisable energy (ME) per kg DM which were fed ad libitum (UMEI; unrestricted ME intake) resulting in four experimental diets (HCP-HP-UMEI, LCP-HP-UMEI, HCP-LP-UMEI and LCP-LP-UMEI). An additional nutritional treatment (HCP-HP-RMEI) restricted intake of the HCP-HP diet to an equivalent ME intake of wethers consuming the LCP-LP-UMEI treatment. Wethers offered the LCP-HP-UMEI, HCP-LP-UMEI and LCP-LP-UMEI treatments consumed 42, 32 and 49% less total DM (P ≤ 0.05), respectively than the HCP-HP-UMEI treatment, and this was not attributable to any physical limitation of the rumen. Plasma concentrations of urea nitrogen and inorganic phosphate indicated that these nutrient deficiencies were successfully established. To assess potential mechanisms, RNA-seq was conducted on samples from the arcuate nucleus (ARC), ventromedial hypothalamus and lateral hypothalamus of the wethers, yielding a total of 301, 8 and 148 differentially expressed genes across all pairwise comparisons, respectively. The expression of NPY, AGRP and CARTPT, known for their regulatory role in mammalian feed intake regulation, had a similar transcriptional response in the ARC of wethers consuming nutrient-deficient treatments and those consuming a ME-restricted treatment, despite these wethers expressing behaviours indicative of satiated and hungry states, respectively. In addition, genes involved with glycolysis (TPI1), the citric acid cycle (CS, OGDH, GLUD1, GOT1) and oxidative phosphorylation (COX5A, ATP5MC1, ATP5F1B, ATP5MC3) were downregulated in the ARC of wethers fed a nutrient deficient (LCP-LP-UMEI) relative to the non-deficient (HCP-HP-UMEI) treatment. In summary, a model for voluntary feed intake restriction was established to determine genome-wide molecular changes in the hypothalamus of young ruminants.

Improving live weight gain of crossbred Limousin bulls with cassava peel silage.

There is a growing demand for beef products across developing countries. Formulating rations to include locally available waste products has the potential to increase the live weight gain (LWG) of cattle and improve the livelihoods of smallholder farmers; however, upper limit inclusion levels of cassava peel products require investigation. An experiment evaluated the effect of using cassava peel silage (CPS) at the DM inclusion levels of 30, 40, 50, 60 and 70% (with the remainder protein meals and maize stover in the diet) on the LWG of crossbred Limousin × Ongole bulls (269 ±â€¯48.8 kg). Thirty bulls, approximately two years of age, were allocated in a completely randomised block design with six blocks based on initial live weight (LW) and five treatments based on level of CPS. The combination of CPS (with 2% urea of the CPS) and protein meals significantly affected LWG with the highest values obtained at levels of 30 and 50% inclusion of CPS (1.16-1.35 kg/day) (P < 0.05). Polynomial analysis of LWG data revealed the optimal LWG is theoretically achieved at 37% CPS with a LWG of 1.31 kg/day; however, LWG was similar from 30 to 50% inclusion levels and then declined. There was little significant difference at CPS inclusion levels of 30-60%, for DM intake (DMI) which ranged from 2.3 to 2.6% LW, organic matter (OM) digestibility (77.8-81.6%), feed conversion ratio (FCR) (6.56-7.56 kg DM/kg LWG) and feed cost of gain (Indonesia rupiah (IDR)/kg LW 18 612 - 21 398). At a high (70%) level of CPS inclusion, these values were markedly changed when compared to the 30% inclusion level of CPS. Feed treatments did not affect rumen pH, NH3-N, concentration or molar percentage of volatile fatty acids or protozoal population (P < 0.05). Rumen pH measured three hours after morning feed ranged from 6.7 to 6.8 and NH3-N ranged from 14.1 to 19.3 mg NH3-N/dl. It was concluded that inclusion of CPS up to 60% mixed with protein meals and urea and 20% maize stover maximised LWG and profitability of the production system.

Formulating rations with cassava meal to promote high live weight gain in crossbred Limousin bulls.

Formulating rations with high energy and protein feeds such as cassava and locally sourced protein meals is an important strategy to increase live weight gain (LWG) of crossbred bulls in Indonesia. Current systems of production for Indonesian smallholders fatten bulls using cut and carry. Formulating a diet for an optimal combination of available feeds will increase production and potential profitability for smallholders. An experiment was conducted to evaluate the effect of using cassava meal in the diet at levels of 70C, 60C, 50C, 40C and 30C% (with most of the remainder being the protein meals) on the LWG of Limousin × Ongole bulls so as to determine the optimum combination of cassava meal and protein meals for LWG. Thirty bulls were allocated in a completely randomized block design with 6 blocks based on initial live weight (LW) and 5 treatments based on level of cassava meal. The combination of cassava meal (with 2% urea) and protein meals significantly affected LWG with the highest (1.35 kg/day) recorded at 40C (40% cassava meal, 40% protein meals and 20% maize stover). The LWG and nutrient intake increased curvilinearly with decreasing cassava meal and increasing protein meals (P < 0.05). Measured cassava meal inclusion in the final ration as a consequence of the changes in intake was 60, 56, 47, 37 and 28% for the designated 70C, 60C, 50C, 40C and 30C treatments, respectively. Dry matter intake reached 96 g/kg0.75 per day or equal to 2.24% LW at this 40% level of inclusion. At the 70C treatment with 60% cassava meal and 9% protein meals, DM digestibility (69.1%) was lowest and that value increased as the proportion of cassava meal decreased and was highest at the 40C treatment (75.8%). Feed treatments significantly affected rumen pH, ammonia N (NH3N) and volatile fatty acid concentrations (P < 0.05). There was no significant effect on protozoal population (P > 0.05). Rumen pH ranged from 6.3 to 6.9. It was concluded that a combination of 40% dried cassava meal and 40% protein meals with roughage (20%) maximized intake and LWG and beyond that level of cassava meal inclusion, LWG and intake decreased markedly.

Milk synthetic response of the bovine mammary gland to an increase in the local concentration of amino acids and acetate.

Rates of secretion of components into milk are a function of precursor concentrations and parameters that describe expression of the milk synthetic enzymes and their sensitivity to precursor concentrations. To establish the enzymatic sensitivities of milk fat yield and mammary acetate utilization to circulating acetate concentration, lactating cows were infused for 10 h with 0 or 40 g of acetate/h in an external iliac artery supplying one udder half. In addition, to investigate the possibility that energy supply influences the milk protein response to an elevated amino acid (AA) concentration, 2 different AA profiles were infused with and without acetate. Six cows, fed a total mixed ration of 21% crude protein ad libitum, were infused with AA at 0 g/h, 30 g/h in the profile of rumen microbes, or 30 g/h in the profile of milk proteins, in a 3 x 2 factorial arrangement with the 2 acetate treatments of 0 and 40 g/h, all in a 6 x 6 Latin square. Amino acid infusion caused a 60% increase, on average, in plasma concentration of AA entering the infused udder half. From the microbial AA profile, 49% of infused AA were taken up by the udder half, 42% of which occurred during the first pass. From the milk AA profile, 44% of infused AA were taken up by the udder half, 50% of which occurred during the first pass. There was an 8% increase in yield of milk protein with AA infusion, representing 7% capture, but no effect of the infused profile. Acetate infusion caused a decrease in the yields of milk protein and lactose when AA were infused, but not when AA were absent. Milk fat yields were not affected, although acetate concentrations in plasma entering the infused udder half increased by 123% and mammary uptakes increased by 128%. Mammary uptakes of long-chain fatty acids and beta-hydroxybutyrate were not affected by acetate infusion, whereas glucose uptakes tended to increase. It was suggested that excess acetate may have been sequestered in adipose tissue in the udder. Yields of both protein and fat in milk showed a low sensitivity to the concentration of their precursors in circulation. It was concluded that the Km in Michaelis-Menten-type equations describing milk synthesis should be assigned a low value, and that the Vmax is regulated to bring about changes in milk yield and composition.

Supplementation of cattle fed tropical grasses with microalgae increases microbial protein production and average daily gain.

A series of 3 experiments were conducted to evaluate the use of microalgae as supplements for ruminants consuming low-CP tropical grasses. In Exp. 1, the chemical composition and in vitro protein degradability of 9 algae species and 4 protein supplements were determined. In Exp. 2, rumen function and microbial protein (MCP) production were determined in steers fed speargrass hay alone or supplemented with , , , or cottonseed meal (CSM). In Exp. 3, DMI and ADG were determined in steers fed speargrass hay alone or supplemented with increasing amounts of NPN (urea combined with ammonia sulfate), CSM, or . In Exp. 1, the CP content of and (675 and 580 g/kg DM) was highest among the algae species and higher than the other protein supplements evaluated, and sp. had the highest crude lipid (CL) content (198 g/kg DM). In Exp. 2, supplementation increased speargrass hay intake, the efficiency of MCP production, the fractional outflow rate of digesta from the rumen, the concentration of NHN, and the molar proportion of branched-chain fatty acids in the rumen fluid of steers above all other treatments. acceptance by steers was low and this resulted in no significant difference to unsupplemented steers for all parameters measured for this algae supplement. In Exp. 3, ADG linearly increased with increasing supplementary N intake from both and NPN, with no difference between the 2 supplements. In contrast, ADG quadratically increased with increasing supplementary N intake from CSM. It was concluded that and may potentially be used as protein sources for cattle grazing low-CP pastures.

The effects of a concurrent infection of Trichostrongylus colubriformis and Ostertagia circumcincta on calcium, phosphorus and magnesium transactions along the digestive tract of lambs.

Twelve lambs, paired on the basis of live weight, were cannulated in the abomasum, proximal jejunum and terminal ileum. Six were infected with 3000 T. colubriformis and 3000 O. circumcincta larvae per day for 18 weeks. The other six lambs were pair-fed to the infected lambs. All animals were offered fresh ryegrass and white clover pasture, cut daily. Dry matter intake, live weight and plasma Ca, P and Mg concentrations were measured throughout the experiment. During weeks 7 and 17 post-infection, digesta flow along the gastrointestinal tract of infected and control animals was measured. Parasitism depressed dry matter intake by 60 per cent and was associated with a reduction in retention of Ca, P and Mg in both infected and control animals. During weeks 7 and 17, parasitism increased the flow of Ca past the proximal jejunum and, during week 17, the flow of P past the terminal ileum. Increased endogenous Ca and P losses, together with a net reduction in absorption of Ca and P, were associated with depressed plasma Ca and P concentrations. These findings, together with evidence of reduced addition of P to anterior regions of the tract, indicate induced Ca and P deficiency. There was evidence for compensatory absorption of Ca and P in the large intestine and distal small intestine, respectively. Magnesium metabolism was apparently not affected by parasitism.

Calcium and phosphorus absorption in lambs exposed to Trichostrongylus colubriformis.

Ten lambs (29 +/- 1.2 kg) reared parasite-free and prepared with rumen, duodenal and ileal cannulae were paired and one of each pair was given a daily oral dose of 2500 Trichostrongylus colubriformis larvae for 14 weeks. Untreated animals received the amount of ration consumed by their infected pair-mates the previous day. During weeks 6 and 12 of infection, all lambs underwent a 7 day calcium (Ca) and phosphorus (P) balance. During weeks 7 and 13, duodenal and ileal samples were collected to determine the amounts of Ca and P entering and leaving the small intestine. The infection caused varying degrees of feed refusal in all infected animals. As a result, the data on Ca and P in excreta and the amounts of Ca and P entering and leaving the small intestine were regressed against dry matter (DM) intake for each group at each period. There were no between-period differences in these relationships. Calcium absorption and retention were unaffected by the stress of infection. Infection affected several aspects of P metabolism. Blood P concentrations were markedly reduced. Absorption of P from the small intestine was greater (P less than 0.01) in control lambs (at 1 kg DM intake 6.6 g per day) than in infected animals (2.2 g P per day), but there was a greater (P less than 0.05) duodenal flow rate of P in control lambs which suggested much higher rates of salivary secretion of P than in infected animals. Phosphorus flow rates at the ileum were greater (P less than 0.01) in infected lambs, despite the lower duodenal flow rates, which indicated a major abnormality (P less than 0.01) in small intestine absorption of P in infected animals; this may have contributed to the growth check experienced by these lambs.

Protein and energy utilization by ruminants at pasture.

Low live weight gain of cattle in the wet season of tropical areas was identified as a major limitation to achieving annual growth rates from tropical pasture systems sufficient to meet new market specifications of young animals of high carcass weight. Both protein and energy are limiting nutrients for growth. Net transfer of feed protein to the intestines is often not complete, and losses occur with grasses and legumes when CP content exceeds 210 g of CP/kg of digestible OM. This protein loss is important because a collation of experimental data indicated that cattle consuming low- and high-quality pasture and silage-based diets all responded to extra protein. The response was less for the higher-quality forage. The role of legumes in supplying this protein was investigated and, unless legumes can increase total DMI by at least 30%, they will not supply sufficient intestinal protein to increase live weight gain by about 300 g/d. The problem with legumes and some grasses is the loss of protein from the rumen, and increasing energy supply to the rumen, either through improved digestibility or energy supplements, is a strategy that could be used to reduce this. Strategies to increase the proportion of escape protein would be successful, but incorporation of lowly degradable protein fractions into legumes may be more difficult because of the level of expression of these protein fractions required for a significant live weight gain response. Cattle entering the wet season usually exhibit compensatory growth and are exposed to high ambient temperatures and often to high humidity. Intestinal protein above that stipulated in feeding standards may be beneficial in these circumstances, and more emphasis should be placed on the ability of legumes to supply protein postruminally. At present the protein delivery capacity of agronomically competitive legumes seems to be inadequate for the higher growth rates required in production systems, and supplements of energy and protein will be needed to achieve these higher targets until new cultivars appear.

Chronic improvement of amino acid nutrition stimulates initiation of global messenger ribonucleic acid translation in tissues of sheep without affecting protein elongation.

Initiation of mRNA translation and elongation of the polypeptide chain are 2 regulated processes responsible for the short-term postprandial acceleration of protein synthesis in animal tissues. It is known that a chronic increase in the absorptive supply of AA stimulates protein synthesis in ruminant animals, but effects on translation initiation and elongation are unknown. To determine whether initiation or elongation phases of global mRNA translation are affected by chronic elevation of AA supply, 24 ewe lambs of 25.9 +/- 2.5 kg of BW were randomly allocated to 4 treatment groups of 6 lambs each. All lambs received a basal diet of barley and hay at 1.2 times maintenance ME intake. Treatments were an intravenous (i.v.) saline infusion as a control, i.v. infusion of 6 essential AA (EAA; Arg, Lys, His, Thr, Met, Cys) for 10 d, i.v. infusion of the same EAA excluding Met and Cys (EAA-SAA) for 10 d, and an oral drench of fishmeal twice daily for 17 d. Fishmeal supplementation supplied an extra 719 mg of N x kg(-0.75) x d(-1) and N retention was increased 519 mg x kg(-0.75) x d(-1) over the control. The EAA treatment supplied an extra 343 mg of N x kg(-0.75) x d(-1) directly into the blood, and N balance was increased by 268 mg x kg(-0.75) x d(-1). Deletion of Met plus Cys from EAA had no effect on N balance. The results indicate that Met plus Cys did not limit body protein gain on the basal diet alone or the basal diet plus 6 AA. Protein fractional synthesis rates in liver, duodenum, skin, rumen, semimembranosus, and LM were measured by a flooding dose procedure using L-[ring-2,6-(3)H]-Phe. Ribosome transit times were estimated from the ratio of nascent to total protein-bound radioactivities. Fishmeal and EAA treatments had no effect on RNA, DNA, or protein contents of tissues, but fractional synthesis rate, translational efficiency, and concentrations of active ribosomes were consistently elevated. Ribosome transit time was not affected by long-term AA supply. We conclude that the chronic stimulation of protein synthesis by long-term i.v. infusion of EAA or supplementation with an undegradable protein source is brought about by an improvement in the rate of initiation of mRNA translation with no change in the rate of polypeptide chain elongation.

Recovery of intravenously infused chromium EDTA and lithium sulphate in the urine of cattle and their use as markers to measure urine volume.

A series of metabolism experiments investigated the recovery of continuous-, intravenously infused chromium complexed with ethylenediamine tetra-acetic acid (CrEDTA) and lithium sulphate in the urine of cattle with a view to using the markers to estimate urine and metabolite output in grazing cattle. The recovery of Cr in urine from these infusions was similar (90%) in metabolism trials when cattle consumed three very contrasting diets: high-grain formulated pellet, lucerne hay (Medicago sativa) or low-quality native grass hay (predominantly Heteropogon contortus). By contrast, Li recovery in urine averaged 46.3 ± 0.40% and 72.6 ± 0.43% for native pasture and lucerne hays, respectively, but was not constant across days. There was negligible transfer of Cr from CrEDTA in blood serum to the rumen or faeces, whereas appreciable quantities of infused Li were found in both. The ratio of urine volume estimated by spot samples and marker dilution of Cr, to urine volume measured gravimetrically, was 1.05. In grazing studies using rumen-fistulated (RF) steers grazing seven different tropical and temperate grass and legume pastures, the ratio of concentrations of purine derivatives (PD) to Cr in spot samples of urine was shown to vary diurnally in the range of 49% to 157% of the average 24 h value. This finding indicated the need for regular sampling of urine to achieve an accurate average value for the PD : Cr ratio in urine for use in estimating urinary PD excretion and hence microbial protein production in the rumen. It was concluded that continuous, intravenous infusion of CrEDTA resulted in a constant recovery of Cr in the urine of cattle across diets and, provided an intensive sampling regime was followed to account for diurnal variation, it would be suitable as a marker to estimate urine volume and urinary output of PD in grazing cattle.

Protein elongation rates in tissues of growing and adult sheep.

To identify the relative roles of translation initiation and elongation in the long term control of protein synthesis in ovine tissues, fractional synthesis rates (FSR) and ribosomal transit times (RTT) were measured in vivo in 24 ewe lambs at 3 levels of intake [maintenance (M), 1.5M, and 2M] and 8 mature ewes at 2M intake. After 17 to 25 d on treatment, animals were given an i.v. flooding dose of l-[ring-2,6-(3)H]phenylalanine and tissues were collected for analysis of radioactivity in free protein, total protein, and nascent ribosome-associated proteins. Ribosome transit time (the inverse of elongation rate) averaged 83, 393, 183, 241, 85, and 113 s for liver, duodenum, skin, rumen, semimembranosus, and LM, respectively. In response to an increased level of intake, protein FSR increased (P < 0.01) in all tissues except rumen and was attributed to greater translational efficiency. There was no effect (P > 0.50) of intake on RTT in these tissues, and the estimated proportion of ribosomes attached to and actively translating mRNA was increased (P < 0.07), indicating that an upregulation of initiation was responsible for the greater FSR. Mature ewes exhibited lower (P < 0.10) protein FSR in all tissues compared with lambs, which was related to a decline in the RNA:protein ratio in all tissues except for liver and duodenum. In all tissues but liver and semimembranosus, RTT increased (P < 0.10) with age. The lower elongation rate was not considered to have influenced the protein synthetic rate, but it caused an increase in the proportion of ribosomes actively translating mRNA. It is anticipated that this work will provide direction to future studies of the molecular mechanisms of chronic FSR control.

The intake, digestion and protein degradation of grazed herbage by early-weaned lambs.

Sixty-four intact lambs and twenty-four lambs fitted with a duodenal cannula were weaned at 6 weeks of age and grazed pure species swards of either lucerne (Medicago sativa), white clover ((Trifolium repens), ryegrass (Lolium perenne) or prairie grass (Bromus catharticus) for 6 weeks. Intake and duodenal digesta flow were estimated when lambs were 8 and 12 weeks of age. Lambs grazing the two legume species grew at a similar rate, as did lambs grazing the two grass species. Legumes promoted a 38% higher growth rate than grasses. The higher growth rate of lambs grazing legumes was associated with a 36% higher digestible organic matter intake (DOMI; 29.5 and 21.7 g/kg body-weight per d for legume and grass respectively) and a 33% higher duodenal non-ammonia-nitrogen (NAN) flow (1.22 and 0.92 g/kg body-weight per d respectively). There was no species difference in the site of organic matter digestion; on average 0.56 of DOMI was apparently digested in the rumen and 0.77 of DOMI was truly digested in the rumen. There was no difference in duodenal NAN flow, relative to DOMI (average, 43 g/kg) or to organic matter apparently digested in the rumen (80 g/kg). Similarly, there was no difference in microbial N flow relative to duodenal NAN (0.50 g/g) and organic matter apparently (41 g/kg) or truly (29 g/kg) digested in the rumen. It was concluded that the higher growth rates achieved by lambs grazing legumes were due to higher intakes which increased the total quantity of nutrients supplied despite more protein being lost in the rumen of lambs consuming legumes.

Nitrogen transactions along the digestive tract of lambs concurrently infected with Trichostrongylus colubriformis and Ostertagia circumcincta.

Twelve lambs, paired on the basis of live weight, were cannulated in the abomasum, in the proximal jejunum approximately 4 m distal to the pylorus and in the terminal ileum. Six were infected with 3000 Trichostrongylus colubriformis and 3000 Ostertagia circumcincta larvae each day for 18 weeks and the remainder were pair-fed to individual infected lambs. All animals were offered ryegrass (Lolium perenne)-white clover (Trifolium repens) pasture, cut daily. Dry matter (DM) intake, live weight, faecal egg concentration, plasma pepsinogen and plasma protein concentrations were measured weekly. During weeks 7 and 17 after commencement of infection, the flow of digesta along the gastrointestinal tract was measured together with enteric plasma loss and true digestion and absorption of 125I-labelled albumin in the small intestine. DM intake was depressed by parasitism, being 1331, (SE 70), 423 (SE 32) and 529 (SE 52) g/d during weeks 3, 7 and 17 respectively. The flow of nitrogen at the proximal jejunum and in faeces was increased by parasitism during week 7 and at the abomasum and ileum during week 17. Plasma protein-N loss (g/d) into the gastrointestinal tract was 0.68 (SE 0.091) and 1.97 (SE 0.139) during week 7, and 0.85 (SE 0.158) and 1.96 (SE 0.396) during week 17, in control and infected sheep respectively. True digestion and absorption of albumin in the proximal small intestine, the site of infection, was very low (mean 0.08) and was not affected by parasitism. Between the abomasum and terminal ileum absorption of albumin was high (mean 0.87) and again was not affected by parasitism. It was calculated that of the total increase in endogenous protein passing from the ileum tract as a result of infection, plasma protein comprised only a small percentage (10-36%). The major proportion of digestion and absorption of protein occurred in the distal small intestine beyond the site of infection and was not affected by infection.

Theoretical considerations in the estimation of rumen fractional outflow rate from various sampling sites in the digestive tract.

The digesta markers 103ruthenium phenanthroline (Ru-P) and 51Cr-EDTA were continuously infused into the rumen of young lambs offered clover hay ad lib. indoors or grazing pure species swards of either lucerne (Medicago sativa), white clover (Trifolium repens), ryegrass (Lolium perenne) or prairie grass (Bromus catharticus). Following the cessation of infusion the fractional outflow rate (FOR) of markers from the rumen was estimated from the rate of decline of marker concentration in rumen and abomasal digesta (Expt 1), abomasal digesta and faeces (Expt 2) and duodenal digesta and faeces (Expt 3). Estimates of FOR from the rumen were similar when based on rumen and abomasal sampling but estimates from abomasal and duodenal sampling were significantly higher than those from faeces. A simulation of a two-compartment model with variable FOR was performed to calculate faecal excretion curves following continuous marker infusion or the administration of a single marker dose into the rumen. Analysis of the descending portion of these theoretical faecal excretion curves yielded values of FOR from the rumen which differed from the values entered in the equations. The magnitude of the difference depended on the ratio of the values of FOR from the caecum and rumen entered in the equation. This suggested that the experimental variation observed in these young lambs was caused by the high FOR from the rumen in relation to the FOR from the caecum-large intestine.

Nitrogen transactions in the digestive tract of lambs exposed to the intestinal parasite, Trichostrongylus colubriformis.

1. Ten 5-month-old lambs (29 (SE 1.2) kg), reared parasite-free and prepared with rumen duodenal and ileal cannulas, were paired and given rations of Ruminant Diet AA6 (90 g/kg live weight0.75) by means of continuous feeders. From 6 months of age one of each pair was dosed daily with 2500 Trichostrongylus colubriformis larvae for 14 weeks. Untreated animals received the amount of ration consumed by their infected pair-mates the previous day. 2. During three periods, [1) the week before and the first 2 weeks of dosing with infected larvae, (2) during weeks 5-7 and (3) during weeks 11-13 of dosing) all lambs underwent a series of experiments to determine their nitrogen balance, the amounts of N leaving the small intestine, the amount of 51CrCl3-labelled plasma protein leaking into the small intestines, and the disappearance of 35S-labelled bacteria from the small intestine. 3. The infection caused varying degrees of feed refusal in all infected animals. As a result the values for N balance and for the flow of N at the ileum during the latter two periods were regressed against dry-matter intakes for each group in each period. 4. The infection caused a reduction (P less than 0.05) in N retention and increased (P less than 0.05) flow of N at the ileum. The increase in N flow at the ileum of infected lambs was greater (P less than 0.01) at weeks 11-13 of dosing (infected-control 3.6 g N/d (standard error of difference (SED) 0.57), P less than 0.01) than at weeks 5-7 of dosing (infected-control 1.5 g N/d (SED 0.57), P less than 0.05). 5. There were no between-treatment or between-period differences in the disappearance of 35S-labelled bacteria from the small intestines of infected or control lambs, but the infection did cause an increase in plasma N leakage during both periods. During weeks 5-7 and 11-13, plasma N leakage in infected lambs was 1.1 g N/d (P less than 0.01) and 1.7 g N/d (P = 0.056) respectively higher than that in the control lambs. 6. A proportion of the endogenous secretions which enter the small intestine is likely to be resorbed before the ileum.(ABSTRACT TRUNCATED AT 400 WORDS)

Estimating the production of faeces by ruminants from faecal marker concentration curves.

The use of faecal marker concentration curves, in conjunction with compartmental analysis, is examined as a method for predicting faecal output in ruminants. Formulae for faecal production are derived for the various multicompartment models currently used to interpret marker concentration data. A comparison of observed and model-derived estimates of faecal dry matter production using three different markers is given for sheep consuming hay or a mixed diet.

Marker concentration patterns of labelled leaf and stem particles in the rumen of cattle grazing bermuda grass (Cynodon dactylon) analysed by reference to a raft model.

Large (>1600 microm), ingestively masticated particles of bermuda grass (Cynodon dactylon L. Pers.) leaf and stem labelled with 169Yb and 144Ce respectively were inserted into the rumen digesta raft of heifers grazing bermuda grass. The concentration of markers in digesta sampled from the raft and ventral rumen were monitored at regular intervals over approximately 144 h. The data from the two sampling sites were simultaneously fitted to two pool (raft and ventral rumen-reticulum) models with either reversible or sequential flow between the two pools. The sequential flow model fitted the data equally as well as the reversible flow model but the reversible flow model was used because of its greater application. The reversible flow model, hereafter called the raft model, had the following features: a relatively slow age-dependent transfer rate from the raft (means for a gamma 2 distributed rate parameter for leaf 0.0740 v. stem 0.0478 h(-1)), a very slow first order reversible flow from the ventral rumen to the raft (mean for leaf and stem 0.010 h(-1)) and a very rapid first order exit from the ventral rumen (mean of leaf and stem 0.44 h(-1)). The raft was calculated to occupy approximately 0.82 total rumen DM of the raft and ventral rumen pools. Fitting a sequential two pool model or a single exponential model individually to values from each of the two sampling sites yielded similar parameter values for both sites and faster rate parameters for leaf as compared with stem, in agreement with the raft model. These results were interpreted as indicating that the raft forms a large relatively inert pool within the rumen. Particles generated within the raft have difficulty escaping but once into the ventral rumen pool they escape quickly with a low probability of return to the raft. It was concluded that the raft model gave a good interpretation of the data and emphasized escape from and movement within the raft as important components of the residence time of leaf and stem particles within the rumen digesta of cattle.

Elevation of feed intake in parasite-infected lambs by central administration of a cholecystokinin receptor antagonist.

The role of cholecystokinin (CCK) in modulating feed intake depression in parasite-infected lambs was investigated using CCK receptor antagonists (L364-718 and loxiglumide). Four experiments were carried out using ewe lambs infected with 4000 Trichostrongylus colubriformis larvae/d or non-infected controls (n8, live weight 25 kg). Animals were fed daily on a nutritionally complete pelleted diet and had free access to water. In the first experiment, infected and non-infected animals were injected subcutaneously with CCK antagonist (100 micrograms L364-718) or carrier alone as a single dose. In the second experiment, CCK antagonist (loxiglumide: 0, 5, 10 or 20 mg/kg live weight) was injected into a jugular vein immediately before feeding. In the third experiment, animals were infused continuously with the CCK antagonist (loxiglumide; 10 mg/kg per h) for 10 min before feeding and for the first 2 h of feeding. In the final experiment, lambs were fitted with an indwelling cerebral ventricular cannula and infused with a CCK antagonist (loxiglumide, 162 micrograms/min), CCK agonist (CCK-8, 2.5 pmol/min), loxiglumide plus CCK-8 or sterile saline solution alone via the cannula for 30 min before feeding and for the first 60 min of feeding. In all the experiments short-term feed intake was recorded at 10 and 15 min intervals for the first and second hours of feeding respectively, then at hourly intervals for the remainder of the 8 h recording period. Peripheral injection with L364-718 or loxiglumide did not elevate feed intake in either the infected or non-infected animals. However, feed intake was increased (P < 0.05) in the short term by central infusion of loxiglumide, this effect being greater in the infected animals and apparently due to an elevation in intake during the second hour of feeding. CCK-8 depressed short term feed intake only in the infected animals (P < 0.05). Total daily feed consumption was not influenced by any of the pharmacological agents. The results indicate an involvement of central CCK receptors in regulation of feed intake depression following gastrointestinal parasitism of sheep and the possibility of a similar role in non-infected sheep. They do not support the singular importance of a peripheral action of CCK in determining satiety.