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1.
Cell ; 141(2): 280-9, 2010 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-20403324

RESUMO

Mitochondria are highly mobile and dynamic organelles that continually fuse and divide. These processes allow mitochondria to exchange contents, including mitochondrial DNA (mtDNA). Here we examine the functions of mitochondrial fusion in differentiated skeletal muscle through conditional deletion of the mitofusins Mfn1 and Mfn2, mitochondrial GTPases essential for fusion. Loss of the mitofusins causes severe mitochondrial dysfunction, compensatory mitochondrial proliferation, and muscle atrophy. Mutant mice have severe mtDNA depletion in muscle that precedes physiological abnormalities. Moreover, the mitochondrial genomes of the mutant muscle rapidly accumulate point mutations and deletions. In a related experiment, we find that disruption of mitochondrial fusion strongly increases mitochondrial dysfunction and lethality in a mouse model with high levels of mtDNA mutations. With its dual function in safeguarding mtDNA integrity and preserving mtDNA function in the face of mutations, mitochondrial fusion is likely to be a protective factor in human disorders associated with mtDNA mutations.


Assuntos
DNA Mitocondrial/genética , Mitocôndrias Musculares/fisiologia , Músculo Esquelético/citologia , Músculo Esquelético/fisiologia , Mutação , Animais , DNA Polimerase gama , DNA Polimerase Dirigida por DNA/metabolismo , Embrião de Mamíferos/metabolismo , Feminino , GTP Fosfo-Hidrolases/metabolismo , Genes Letais , Masculino , Camundongos , Mitocôndrias Musculares/genética , Miopatias Mitocondriais/metabolismo , Proteínas Mitocondriais/genética
2.
Cell ; 143(5): 802-12, 2010 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-21094524

RESUMO

Caloric restriction (CR) extends the life span and health span of a variety of species and slows the progression of age-related hearing loss (AHL), a common age-related disorder associated with oxidative stress. Here, we report that CR reduces oxidative DNA damage in multiple tissues and prevents AHL in wild-type mice but fails to modify these phenotypes in mice lacking the mitochondrial deacetylase Sirt3, a member of the sirtuin family. In response to CR, Sirt3 directly deacetylates and activates mitochondrial isocitrate dehydrogenase 2 (Idh2), leading to increased NADPH levels and an increased ratio of reduced-to-oxidized glutathione in mitochondria. In cultured cells, overexpression of Sirt3 and/or Idh2 increases NADPH levels and protects from oxidative stress-induced cell death. Therefore, our findings identify Sirt3 as an essential player in enhancing the mitochondrial glutathione antioxidant defense system during CR and suggest that Sirt3-dependent mitochondrial adaptations may be a central mechanism of aging retardation in mammals.


Assuntos
Envelhecimento/metabolismo , Restrição Calórica , Perda Auditiva/prevenção & controle , Mitocôndrias/metabolismo , Estresse Oxidativo , Sirtuína 3/metabolismo , Animais , Antioxidantes/metabolismo , Dano ao DNA , Feminino , Glutationa/metabolismo , Isocitrato Desidrogenase/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Sirtuína 3/genética
3.
Mod Pathol ; 36(12): 100332, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37716507

RESUMO

Somatostatin receptor type 2 (SSTR2) and thyroid-stimulating hormone receptor (TSHR) display variable expression in primary thyroid tumors and have been implicated as theranostic targets. This study was designed to explore the differential expression of SSTR2 and TSHR in oncocytic (Hurthle cell) carcinoma (OC) vs oncocytic adenoma (OA). We performed a retrospective review for oncocytic neoplasms treated at our institution from 2012 to 2019. Formalin-fixed paraffin-embedded tissue blocks were used for tissue microarray construction. Tissue microarray blocks were cut into 5-µm sections and stained with anti-SSTR2 and anti-TSHR antibodies. Immunostains were analyzed by 3 independent pathologists. χ2 and logistic regression analysis were used to analyze clinical and pathologic variables. Sixty-seven specimens were analyzed with 15 OA and 52 OC. The mean age was 57 years, 61.2% were women, and 70% were White. SSTR2 positivity was noted in 2 OA (13%) and 15 OC (28%; 10 primary, 4 recurrent, and 1 metastatic) (P = .22). TSHR positivity was noted in 11 OA (73%) and 32 OC (62%; 31 primary and 1 metastatic) (P = .40). Those who presented with or developed clinical recurrence/metastasis were more likely to be SSTR2-positive (50% vs 21%; P = .04) and TSHR-negative (64.3% vs 28.9%; P = .02) than primary OC patients. Widely invasive OC was more likely to be SSTR2-positive compared to all other OC subtypes (minimally invasive and angioinvasive) (P = .003). For all patients with OC, TSHR positivity was inversely correlated with SSTR2 positivity (odds ratio, 0.12; CI, 0.03-0.43; P = .006). This relationship was not seen in the patients with OA (odds ratio, 0.30; CI, 0.01-9.14; P = .440). Our results show that recurrent/metastatic OC was more likely to be SSTR2-positive and TSHR-negative than primary OC. Patients with OC displayed a significant inverse relationship between SSTR2 and TSHR expression that was not seen in patients with OA. This may be a key relationship that can be used to prognosticate and treat OCs.


Assuntos
Neoplasias Epiteliais e Glandulares , Neoplasias da Glândula Tireoide , Humanos , Feminino , Pessoa de Meia-Idade , Masculino , Receptores da Tireotropina , Prognóstico , Neoplasias da Glândula Tireoide/patologia , Tireotropina
4.
Cell ; 135(5): 907-18, 2008 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-19041753

RESUMO

Genomic instability and alterations in gene expression are hallmarks of eukaryotic aging. The yeast histone deacetylase Sir2 silences transcription and stabilizes repetitive DNA, but during aging or in response to a DNA break, the Sir complex relocalizes to sites of genomic instability, resulting in the desilencing of genes that cause sterility, a characteristic of yeast aging. Using embryonic stem cells, we show that mammalian Sir2, SIRT1, represses repetitive DNA and a functionally diverse set of genes across the mouse genome. In response to DNA damage, SIRT1 dissociates from these loci and relocalizes to DNA breaks to promote repair, resulting in transcriptional changes that parallel those in the aging mouse brain. Increased SIRT1 expression promotes survival in a mouse model of genomic instability and suppresses age-dependent transcriptional changes. Thus, DNA damage-induced redistribution of SIRT1 and other chromatin-modifying proteins may be a conserved mechanism of aging in eukaryotes.


Assuntos
Envelhecimento/genética , Cromatina/metabolismo , Instabilidade Genômica , Sirtuínas/genética , Animais , Encéfalo/metabolismo , Linhagem Celular Tumoral , Quebras de DNA de Cadeia Dupla , Reparo do DNA , Células-Tronco Embrionárias , Técnicas de Inativação de Genes , Humanos , Linfoma/metabolismo , Camundongos , Dados de Sequência Molecular , Estresse Oxidativo , Sirtuína 1 , Organismos Livres de Patógenos Específicos , Neoplasias do Timo/metabolismo , Leveduras/citologia , Leveduras/metabolismo
5.
Mol Cell ; 49(1): 186-99, 2013 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-23201123

RESUMO

Calorie restriction (CR) extends life span in diverse species. Mitochondria play a key role in CR adaptation; however, the molecular details remain elusive. We developed and applied a quantitative mass spectrometry method to probe the liver mitochondrial acetyl-proteome during CR versus control diet in mice that were wild-type or lacked the protein deacetylase SIRT3. Quantification of 3,285 acetylation sites-2,193 from mitochondrial proteins-rendered a comprehensive atlas of the acetyl-proteome and enabled global site-specific, relative acetyl occupancy measurements between all four experimental conditions. Bioinformatic and biochemical analyses provided additional support for the effects of specific acetylation on mitochondrial protein function. Our results (1) reveal widespread reprogramming of mitochondrial protein acetylation in response to CR and SIRT3, (2) identify three biochemically distinct classes of acetylation sites, and (3) provide evidence that SIRT3 is a prominent regulator in CR adaptation by coordinately deacetylating proteins involved in diverse pathways of metabolism and mitochondrial maintenance.


Assuntos
Restrição Calórica , Proteínas Mitocondriais/metabolismo , Proteoma/metabolismo , Sirtuína 3/fisiologia , Acetilcoenzima A/metabolismo , Acetilação , Adaptação Fisiológica , Motivos de Aminoácidos , Sequência de Aminoácidos , Aminoácidos/metabolismo , Animais , Metabolismo dos Carboidratos , Células Cultivadas , Cromatografia por Troca Iônica , Análise por Conglomerados , Sequência Consenso , Expressão Gênica , Genes Mitocondriais , Fígado/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Mitocôndrias Hepáticas/metabolismo , Proteínas Mitocondriais/química , Proteínas Mitocondriais/isolamento & purificação , Dados de Sequência Molecular , Fragmentos de Peptídeos/química , Processamento de Proteína Pós-Traducional , Proteoma/química , Proteoma/isolamento & purificação , Sirtuína 3/química , Sirtuína 3/isolamento & purificação , Sirtuína 3/metabolismo , Coloração e Rotulagem , Espectrometria de Massas em Tandem
6.
Horm Behav ; 115: 104556, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31310763

RESUMO

There is evidence of reduced adiposity in rodents living in a large cages (LC) as compared to animals housed in small cages (SC). Because spontaneous physical activity (SPA) provides an important portion of the total daily energy expenditure, an increase of SPA in rodents kept in LC could explain their reduced body fat accumulation. The relationship between SPA and components of physical fitness (i.e. aerobic and anaerobic fitness and body leanness) has not been previously determined. We examined the effects of eight weeks of LC exposure on SPA, body composition, feeding behavior, as well as aerobic and anaerobic running capacity in adult C57BL/6J mice. Male mice were housed in cages of two different sizes for 8 weeks: a small (SC, n = 10) and large (LC n = 10) cages with 1320 cm2 and 4800 cm2 floor space, respectively. SPA was measured gravimetrically, and food and water intake were recorded daily. Mice had critical velocity (CV) and anaerobic running capacity (ARC) evaluated at the beginning, middle course (4th week) and at the end of study (8th week). Despite non-significant differences in each week LC-mice were more active than SC-mice by considering all SPA values obtained in the entire period of 8 weeks. The difference in SPA over the whole day was mainly due to light phase activity, but also due to activity at dark period (from 6 pm to 9 pm and from 5 am to 6 am). LC-mice also exhibited higher food and water intake over the entire 8-wk period. LC-mice had lower content of fat mass (% of the eviscerated carcass) than SC-mice (SC: 8.4 ±â€¯0.4 vs LC: 6.3 ±â€¯0.3, p < 0.05). LC-mice also exhibited reduced epididymal fat pads (% of body mass) compared to SC-mice (SC: 1.3 ±â€¯0.1 vs LC: 0.9 ±â€¯0.1, p < 0.05) and retroperitoneal fat pads (SC: 0.4 ±â€¯0.05 vs LC: 0.2 ±â€¯0.02, p < 0.05). The LC-group showed significantly higher critical velocity than SC-group at the fourth week (SC: 14.9 ±â€¯0.6 m·min-1 vs LC: 18.0 ±â€¯0.3 m·min-1, p < 0.05) and eighth week (SC: 17.1 ±â€¯0.5 m·min-1 vs LC: 18.8 ±â€¯0.6 m·min-1, p < 0.05). Our findings demonstrate that eight weeks of LC housing increases SPA of C57BL/6J mice, and this may lead to reduced fat accumulation as well as higher aerobic fitness. Importantly, our study implies that SC limits SPA, possibly generating experimental artifacts in long-term rodent studies.


Assuntos
Adiposidade/fisiologia , Comportamento Animal/fisiologia , Abrigo para Animais , Locomoção/fisiologia , Atividade Motora/fisiologia , Animais , Masculino , Camundongos , Camundongos Endogâmicos C57BL
7.
Blood ; 125(1): 162-74, 2015 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-25411424

RESUMO

Somatic mitochondrial DNA (mtDNA) mutations contribute to the pathogenesis of age-related disorders, including myelodysplastic syndromes (MDS). The accumulation of mitochondria harboring mtDNA mutations in patients with these disorders suggests a failure of normal mitochondrial quality-control systems. The mtDNA-mutator mice acquire somatic mtDNA mutations via a targeted defect in the proofreading function of the mtDNA polymerase, PolgA, and develop macrocytic anemia similar to that of patients with MDS. We observed an unexpected defect in clearance of dysfunctional mitochondria at specific stages during erythroid maturation in hematopoietic cells from aged mtDNA-mutator mice. Mechanistically, aberrant activation of mechanistic target of rapamycin signaling and phosphorylation of uncoordinated 51-like kinase (ULK) 1 in mtDNA-mutator mice resulted in proteasome-mediated degradation of ULK1 and inhibition of autophagy in erythroid cells. To directly evaluate the consequence of inhibiting autophagy on mitochondrial function in erythroid cells harboring mtDNA mutations in vivo, we deleted Atg7 from erythroid progenitors of wild-type and mtDNA-mutator mice. Genetic disruption of autophagy did not cause anemia in wild-type mice but accelerated the decline in mitochondrial respiration and development of macrocytic anemia in mtDNA-mutator mice. These findings highlight a pathological feedback loop that explains how dysfunctional mitochondria can escape autophagy-mediated degradation and propagate in cells predisposed to somatic mtDNA mutations, leading to disease.


Assuntos
Anemia/genética , Autofagia/genética , DNA Mitocondrial/genética , Eritrócitos/citologia , Serina-Treonina Quinases TOR/metabolismo , Envelhecimento , Animais , Separação Celular , DNA Polimerase gama , DNA Polimerase Dirigida por DNA/metabolismo , Células Eritroides/metabolismo , Citometria de Fluxo , Heterozigoto , Camundongos , Mitocôndrias/metabolismo , Mutação , Síndromes Mielodisplásicas/genética , Consumo de Oxigênio , Fenótipo , Fosforilação , Complexo de Endopeptidases do Proteassoma/metabolismo , Ribossomos/metabolismo
8.
J Biol Chem ; 290(36): 22061-75, 2015 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-26183775

RESUMO

Transfer of cardiac progenitor cells (CPCs) improves cardiac function in heart failure patients. However, CPC function is reduced with age, limiting their regenerative potential. Aging is associated with numerous changes in cells including accumulation of mitochondrial DNA (mtDNA) mutations, but it is unknown how this impacts CPC function. Here, we demonstrate that acquisition of mtDNA mutations disrupts mitochondrial function, enhances mitophagy, and reduces the replicative and regenerative capacities of the CPCs. We show that activation of differentiation in CPCs is associated with expansion of the mitochondrial network and increased mitochondrial oxidative phosphorylation. Interestingly, mutant CPCs are deficient in mitochondrial respiration and rely on glycolysis for energy. In response to differentiation, these cells fail to activate mitochondrial respiration. This inability to meet the increased energy demand leads to activation of cell death. These findings demonstrate the consequences of accumulating mtDNA mutations and the importance of mtDNA integrity in CPC homeostasis and regenerative potential.


Assuntos
Proliferação de Células/genética , DNA Mitocondrial/genética , Mutação , Células-Tronco/metabolismo , Animais , Western Blotting , Diferenciação Celular/genética , Sobrevivência Celular/genética , Células Cultivadas , DNA Polimerase gama , DNA Polimerase Dirigida por DNA/genética , DNA Polimerase Dirigida por DNA/metabolismo , Complexo IV da Cadeia de Transporte de Elétrons/genética , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Feminino , Masculino , Camundongos Endogâmicos C57BL , Camundongos Knockout , Microscopia Eletrônica de Transmissão , Microscopia de Fluorescência , Mitocôndrias/genética , Mitocôndrias/metabolismo , Mitocôndrias/ultraestrutura , Miocárdio/citologia , Miocárdio/metabolismo , Biogênese de Organelas , Fosforilação Oxidativa , Consumo de Oxigênio/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa
9.
Nat Genet ; 39(4): 540-3, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17334366

RESUMO

Whether mitochondrial mutations cause mammalian aging, or are merely correlated with it, is an area of intense debate. Here, we use a new, highly sensitive assay to redefine the relationship between mitochondrial mutations and age. We measured the in vivo rate of change of the mitochondrial genome at a single-base pair level in mice, and we demonstrate that the mutation frequency in mouse mitochondria is more than ten times lower than previously reported. Although we observed an 11-fold increase in mitochondrial point mutations with age, we report that a mitochondrial mutator mouse was able to sustain a 500-fold higher mutation burden than normal mice, without any obvious features of rapidly accelerated aging. Thus, our results strongly indicate that mitochondrial mutations do not limit the lifespan of wild-type mice.


Assuntos
Longevidade/genética , Mitocôndrias/genética , Mutação Puntual/fisiologia , Envelhecimento/genética , Animais , Células Cultivadas , DNA Polimerase gama , DNA Polimerase Dirigida por DNA/genética , DNA Polimerase Dirigida por DNA/metabolismo , Camundongos , Camundongos Transgênicos , Mitocôndrias/enzimologia , Mitocôndrias/metabolismo
10.
Hum Mol Genet ; 21(10): 2288-97, 2012 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-22357654

RESUMO

Aging is an intricate process that increases susceptibility to sarcopenia and cardiovascular diseases. The accumulation of mitochondrial DNA (mtDNA) mutations is believed to contribute to mitochondrial dysfunction, potentially shortening lifespan. The mtDNA mutator mouse, a mouse model with a proofreading-deficient mtDNA polymerase γ, was shown to develop a premature aging phenotype, including sarcopenia, cardiomyopathy and decreased lifespan. This phenotype was associated with an accumulation of mtDNA mutations and mitochondrial dysfunction. We found that increased expression of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), a crucial regulator of mitochondrial biogenesis and function, in the muscle of mutator mice increased mitochondrial biogenesis and function and also improved the skeletal muscle and heart phenotypes of the mice. Deep sequencing analysis of their mtDNA showed that the increased mitochondrial biogenesis did not reduce the accumulation of mtDNA mutations but rather caused a small increase. These results indicate that increased muscle PGC-1α expression is able to improve some premature aging phenotypes in the mutator mice without reverting the accumulation of mtDNA mutations.


Assuntos
Envelhecimento/metabolismo , DNA Mitocondrial/metabolismo , Mitocôndrias/metabolismo , Mutação , Fenótipo , Animais , Camundongos , Camundongos Transgênicos , Músculo Esquelético/metabolismo , Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo , Transativadores/genética , Transativadores/metabolismo , Fatores de Transcrição
11.
Oncologist ; 19(11): 1148-55, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25260367

RESUMO

Anaplastic thyroid cancer (ATC), accounting for less than 2% of all thyroid cancer, is responsible for the majority of death from all thyroid malignancies and has a median survival of 6 months. The resistance of ATC to conventional thyroid cancer therapies, including radioiodine and thyroid-stimulating hormone suppression, contributes to the very poor prognosis of this malignancy. This review will cover several cellular signaling pathways and mechanisms, including RET/PTC, RAS, BRAF, Notch, p53, and histone deacetylase, which are identified to play roles in the transformation and dedifferentiation process, and therapies that target these pathways. Lastly, novel approaches and agents involving the Notch1 pathway, nuclear factor κB, Trk-fused gene, cancer stem-like cells, mitochondrial mutation, and tumor immune microenvironment are discussed. With a better understanding of the biological process and treatment modality, the hope is to improve ATC outcome in the future.


Assuntos
Carcinoma Anaplásico da Tireoide/metabolismo , Carcinoma Anaplásico da Tireoide/terapia , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Terapia Genética , Inibidores de Histona Desacetilases/farmacologia , Histona Desacetilases/metabolismo , Humanos , Terapia de Alvo Molecular , NF-kappa B/metabolismo , Inibidores de Proteínas Quinases/farmacologia , Proteínas Proto-Oncogênicas c-ret/genética , Proteínas Proto-Oncogênicas c-ret/metabolismo , Receptor Notch1/metabolismo , Transdução de Sinais , Carcinoma Anaplásico da Tireoide/patologia , Quinases raf/genética , Quinases raf/metabolismo , Proteínas ras/genética , Proteínas ras/metabolismo
12.
Proc Natl Acad Sci U S A ; 108(21): 8779-84, 2011 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-21555558

RESUMO

Diabetes and the development of its complications have been associated with mitochondrial DNA (mtDNA) dysfunction, but causal relationships remain undetermined. With the objective of testing whether increased mtDNA mutations exacerbate the diabetic phenotype, we have compared mice heterozygous for the Akita diabetogenic mutation (Akita) with mice homozygous for the D257A mutation in mitochondrial DNA polymerase gamma (Polg) or with mice having both mutations (Polg-Akita). The Polg-D257A protein is defective in proofreading and increases mtDNA mutations. At 3 mo of age, the Polg-Akita and Akita male mice were equally hyperglycemic. Unexpectedly, as the Polg-Akita males aged to 9 mo, their diabetic symptoms decreased. Thus, their hyperglycemia, hyperphagia and urine output declined significantly. The decrease in their food intake was accompanied by increased plasma leptin and decreased plasma ghrelin, while hypothalamic expression of the orexic gene, neuropeptide Y, was lower and expression of the anorexic gene, proopiomelanocortin, was higher. Testis function progressively worsened with age in the double mutants, and plasma testosterone levels in 9-mo-old Polg-Akita males were significantly reduced compared with Akita males. The hyperglycemia and hyperphagia returned in aged Polg-Akita males after testosterone administration. Hyperglycemia-associated distal tubular damage in the kidney also returned, and Polg-D257A-associated proximal tubular damage was enhanced. The mild diabetes of female Akita mice was not affected by the Polg-D257A mutation. We conclude that reduced diabetic symptoms of aging Polg-Akita males results from appetite suppression triggered by decreased testosterone associated with damage to the Leydig cells of the testis.


Assuntos
Apetite/genética , DNA Mitocondrial/genética , DNA Polimerase Dirigida por DNA/genética , Diabetes Mellitus/genética , Camundongos Endogâmicos/genética , Mutação , Envelhecimento , Animais , DNA Polimerase gama , Diabetes Mellitus/patologia , Diabetes Mellitus/fisiopatologia , Feminino , Hiperglicemia , Células Intersticiais do Testículo/patologia , Masculino , Camundongos , Fenótipo , Edição de RNA/genética , Testículo/patologia , Testosterona/sangue
13.
Proc Natl Acad Sci U S A ; 108(10): 4135-40, 2011 03 08.
Artigo em Inglês | MEDLINE | ID: mdl-21368114

RESUMO

A causal role for mitochondrial DNA (mtDNA) mutagenesis in mammalian aging is supported by recent studies demonstrating that the mtDNA mutator mouse, harboring a defect in the proofreading-exonuclease activity of mitochondrial polymerase gamma, exhibits accelerated aging phenotypes characteristic of human aging, systemic mitochondrial dysfunction, multisystem pathology, and reduced lifespan. Epidemiologic studies in humans have demonstrated that endurance training reduces the risk of chronic diseases and extends life expectancy. Whether endurance exercise can attenuate the cumulative systemic decline observed in aging remains elusive. Here we show that 5 mo of endurance exercise induced systemic mitochondrial biogenesis, prevented mtDNA depletion and mutations, increased mitochondrial oxidative capacity and respiratory chain assembly, restored mitochondrial morphology, and blunted pathological levels of apoptosis in multiple tissues of mtDNA mutator mice. These adaptations conferred complete phenotypic protection, reduced multisystem pathology, and prevented premature mortality in these mice. The systemic mitochondrial rejuvenation through endurance exercise promises to be an effective therapeutic approach to mitigating mitochondrial dysfunction in aging and related comorbidities.


Assuntos
Envelhecimento/fisiologia , DNA Mitocondrial/genética , Mitocôndrias/fisiologia , Condicionamento Físico Animal , Resistência Física , Mutação Puntual , Envelhecimento/genética , Animais , Apoptose , Dosagem de Genes , Camundongos , Camundongos Mutantes , Estresse Oxidativo
15.
Circ Res ; 108(7): 837-46, 2011 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-21311045

RESUMO

RATIONALE: Mitochondrial dysfunction has been implicated in several cardiovascular diseases; however, the roles of mitochondrial oxidative stress and DNA damage in hypertensive cardiomyopathy are not well understood. OBJECTIVE: We evaluated the contribution of mitochondrial reactive oxygen species (ROS) to cardiac hypertrophy and failure by using genetic mouse models overexpressing catalase targeted to mitochondria and to peroxisomes. METHODS AND RESULTS: Angiotensin II increases mitochondrial ROS in cardiomyocytes, concomitant with increased mitochondrial protein carbonyls, mitochondrial DNA deletions, increased autophagy and signaling for mitochondrial biogenesis in hearts of angiotensin II-treated mice. The causal role of mitochondrial ROS in angiotensin II-induced cardiomyopathy is shown by the observation that mice that overexpress catalase targeted to mitochondria, but not mice that overexpress wild-type peroxisomal catalase, are resistant to cardiac hypertrophy, fibrosis and mitochondrial damage induced by angiotensin II, as well as heart failure induced by overexpression of Gαq. Furthermore, primary damage to mitochondrial DNA, induced by zidovudine administration or homozygous mutation of mitochondrial polymerase γ, is also shown to contribute directly to the development of cardiac hypertrophy, fibrosis and failure. CONCLUSIONS: These data indicate the critical role of mitochondrial ROS in cardiac hypertrophy and failure and support the potential use of mitochondrial-targeted antioxidants for prevention and treatment of hypertensive cardiomyopathy.


Assuntos
Angiotensina II/farmacologia , Cardiomegalia/fisiopatologia , Subunidades alfa Gq-G11 de Proteínas de Ligação ao GTP/metabolismo , Insuficiência Cardíaca/fisiopatologia , Mitocôndrias Cardíacas/fisiologia , Estresse Oxidativo/fisiologia , Angiotensina II/efeitos adversos , Animais , Cardiomegalia/induzido quimicamente , Catalase/genética , Catalase/metabolismo , Dano ao DNA/fisiologia , DNA Mitocondrial/efeitos dos fármacos , Subunidades alfa Gq-G11 de Proteínas de Ligação ao GTP/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Insuficiência Cardíaca/metabolismo , Camundongos , Camundongos Transgênicos , Modelos Animais , Miócitos Cardíacos/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Inibidores da Transcriptase Reversa/farmacologia , Zidovudina/farmacologia
16.
Life Sci ; 317: 121443, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36709910

RESUMO

There is a scarcity of data regarding the acclimation to high altitude (hypoxic environment) accompanied by training at low altitude (normoxic conditions), the so-called "living high-training low" (LHTL) model in rodents. We aimed to investigate the effects of aerobic training on C57BL/6J mice living in normoxic (NOR) or hypoxic (HYP) environments on several parameters, including critical velocity (CV), a parameter regarded as a measure of aerobic capacity, on monocarboxylate transporters (MCTs) in muscles and hypothalamus, as well as on hematological parameters and body temperature. In each environment, mice were divided into non-trained (N) and trained (T). Forty rodents were distributed into the following experimental groups (N-NOR; T-NOR; N-HYP and T-HYP). HYP groups were in a normobaric tent where oxygen-depleted air was pumped from a hypoxia generator set an inspired oxygen fraction [FiO2] of 14.5 %. The HYP-groups were kept (18 h per day) in a normobaric tent for consecutive 8-weeks. Training sessions were conducted in normoxic conditions ([FiO2] = 19.5 %), 5 times per week (40 min per session) at intensity equivalent to 80 % of CV. In summary, eight weeks of LHTL did not promote a greater improvement in the CV, protein expression of MCTs in different tissues when compared to the application of training alone. The LHTL model increased red blood cells count, but reduced hemoglobin per erythrocyte was found in mice exposed to LHTL. Although the LHTL did not have a major effect on thermographic records, exercise-induced hyperthermia (in the head) was attenuated in HYP groups when compared to NOR groups.


Assuntos
Equilíbrio Ácido-Base , Hipóxia , Animais , Camundongos , Camundongos Endogâmicos C57BL , Hipóxia/metabolismo , Oxigênio , Tolerância ao Exercício/fisiologia , Consumo de Oxigênio/fisiologia
17.
Am J Physiol Gastrointest Liver Physiol ; 302(9): G914-24, 2012 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-22345551

RESUMO

Changes in intestinal absorption of nutrients are important aspects of the aging process. To address this issue, we investigated the impact of accelerated mitochondrial DNA mutations on the stem/progenitor cells in the crypts of Lieberkühn in mice homozygous for a mitochondrial DNA polymerase gamma mutation, Polg(D257A), that exhibit accelerated aging phenotype. As early as 3-7 mo of age, the small intestine was significantly enlarged in the PolgD257A mice. The crypts of the PolgD257A mice contained 20% more cells than those of their wild-type littermates and exhibited a 10-fold increase in cellular apoptosis primarily in the stem/progenitor cell zones. Actively dividing cells were proportionally increased, yet a significantly smaller proportion of cells was in the S phase of the cell cycle. Stem cell-derived organoids from PolgD257A mice failed to develop fully in culture and exhibited fewer crypt units, indicating an impact of the mutation on the intestinal epithelial stem/progenitor cell maintenance. In addition, epithelial cell migration along the crypt-villus axis was slowed and less organized, and the ATP content in the villi was significantly reduced. On a high-fat, high-carbohydrate diet, PolgD257A mice showed significantly restricted absorption of excess lipids accompanied by an increase in fecal steatocrits. We conclude that the PolgD257A mutation causes cell cycle dysregulation in the crypts leading to the age-associated changes in the morphology of the small intestine and contributes to the restricted absorption of dietary lipids.


Assuntos
Ciclo Celular/genética , DNA Polimerase Dirigida por DNA/genética , Gorduras na Dieta/metabolismo , Absorção Intestinal/genética , Mutação/genética , Células-Tronco/citologia , Células-Tronco/fisiologia , Animais , Contagem de Células , DNA Polimerase gama , Absorção Intestinal/fisiologia , Masculino , Camundongos , Camundongos Transgênicos , Edição de RNA/genética
18.
Proc Natl Acad Sci U S A ; 106(46): 19432-7, 2009 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-19901338

RESUMO

Age-related hearing loss (AHL), known as presbycusis, is a universal feature of mammalian aging and is the most common sensory disorder in the elderly population. The molecular mechanisms underlying AHL are unknown, and currently there is no treatment for the disorder. Here we report that C57BL/6J mice with a deletion of the mitochondrial pro-apoptotic gene Bak exhibit reduced age-related apoptotic cell death of spiral ganglion neurons and hair cells in the cochlea, and prevention of AHL. Oxidative stress induces Bak expression in primary cochlear cells, and Bak deficiency prevents apoptotic cell death. Furthermore, a mitochondrially targeted catalase transgene suppresses Bak expression in the cochlea, reduces cochlear cell death, and prevents AHL. Oral supplementation with the mitochondrial antioxidants alpha-lipoic acid and coenzyme Q(10) also suppresses Bak expression in the cochlea, reduces cochlear cell death, and prevents AHL. Thus, induction of a Bak-dependent mitochondrial apoptosis program in response to oxidative stress is a key mechanism of AHL in C57BL/6J mice.


Assuntos
Apoptose , Mitocôndrias/metabolismo , Estresse Oxidativo/genética , Presbiacusia/genética , Proteína Killer-Antagonista Homóloga a bcl-2/biossíntese , Fatores Etários , Animais , Antioxidantes/farmacologia , Apoptose/efeitos dos fármacos , Cóclea/metabolismo , Cóclea/patologia , Dano ao DNA/efeitos dos fármacos , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Estresse Oxidativo/efeitos dos fármacos , Presbiacusia/patologia , Proteína Killer-Antagonista Homóloga a bcl-2/genética
19.
Aging Cell ; 21(12): e13721, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36199173

RESUMO

Mitochondrial NAD+ -dependent protein deacetylase Sirtuin3 (SIRT3) has been proposed to mediate calorie restriction (CR)-dependent metabolic regulation and lifespan extension. Here, we investigated the role of SIRT3 in CR-mediated longevity, mitochondrial function, and aerobic fitness. We report that SIRT3 is required for whole-body aerobic capacity but is dispensable for CR-dependent lifespan extension. Under CR, loss of SIRT3 (Sirt3-/- ) yielded a longer overall and maximum lifespan as compared to Sirt3+/+ mice. This unexpected lifespan extension was associated with altered mitochondrial protein acetylation in oxidative metabolic pathways, reduced mitochondrial respiration, and reduced aerobic exercise capacity. Also, Sirt3-/- CR mice exhibit lower spontaneous activity and a trend favoring fatty acid oxidation during the postprandial period. This study shows the uncoupling of lifespan and healthspan parameters (aerobic fitness and spontaneous activity) and provides new insights into SIRT3 function in CR adaptation, fuel utilization, and aging.


Assuntos
Restrição Calórica , Longevidade , Sirtuína 3 , Animais , Masculino , Camundongos , Acetilação , Envelhecimento/metabolismo , Longevidade/genética , Mitocôndrias/metabolismo , Sirtuína 3/genética , Sirtuína 3/metabolismo , Estresse Oxidativo/genética
20.
Blood ; 114(19): 4045-53, 2009 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-19734452

RESUMO

Recent reports describe hematopoietic abnormalities in mice with targeted instability of the mitochondrial genome. However, these abnormalities have not been fully described. We demonstrate that mutant animals develop an age-dependent, macrocytic anemia with abnormal erythroid maturation and megaloblastic changes, as well as profound defects in lymphopoiesis. Mice die of severe fatal anemia at 15 months of age. Bone-marrow transplantation studies demonstrate that these abnormalities are intrinsic to the hematopoietic compartment and dependent upon the age of donor hematopoietic stem cells. These abnormalities are phenotypically similar to those found in patients with refractory anemia, suggesting that, in some cases, the myelodysplastic syndromes are caused by abnormalities of mitochondrial function.


Assuntos
Anemia Megaloblástica/etiologia , Linfopoese , Doenças Mitocondriais/complicações , Síndromes Mielodisplásicas/etiologia , Fatores Etários , Anemia Megaloblástica/genética , Anemia Megaloblástica/patologia , Animais , Transplante de Medula Óssea , DNA Polimerase gama , DNA Polimerase Dirigida por DNA/genética , Modelos Animais de Doenças , Células Eritroides/patologia , Eritropoese/genética , Genoma Mitocondrial , Humanos , Linfopoese/genética , Camundongos , Camundongos Mutantes , Doenças Mitocondriais/genética , Doenças Mitocondriais/patologia , Síndromes Mielodisplásicas/genética , Síndromes Mielodisplásicas/patologia , Mutação Puntual
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