RESUMO
In this paper, aboveground biomass and basic nutrients removal, nitrogen (N) and phosphorus (P), was analyzed by the use of reed as the main component of Constructed Wetland System (CWS) "Glozan". In almost ideal conditions of temperate continental climate, with favorable substrate humidity, due to the constant inflow of municipal wastewater, reed populations reach a high density, on average 217 ind/m2. The reed produces significant aboveground biomass, fresh weight (FW) of 144.21 g/plant and dry weight (DW) of 77.04 g/plant, with the largest share being per tree (87.49 g FW/plant, 48.17 g DW/plant), then leaf (49.45 g FW/plant, 24.89 g DW/plant) and the smallest inflorescence (7.27 g FW/plant, 3.99 g DW/plant). The results obtained in this way indicate that the largest amount of nitrogen was removed by leaves, then by stems and, the smallest by inflorescences, 181.07 g/m2, 97.73 g/m2, 23.41 g/m2, respectively. Thus, an average of 302.21 g/m2 of nitrogen was removed by the entire aboveground part of the reed. Also, the largest amount of phosphorus was removed by leaves, then by stems, and the smallest by inflorescences, 5.72 g/m2, 4.82 g/m2 and 2.57 g/m2, respectively, while the entire aboveground part of the reed is on average about 13.11 g/m2.
The contribution of this paper is reflected in the obtained results for population density, reed biomass, and reed efficiency in the process of accumulation and removal of nitrogen and phosphorus as the main factors of accelerated eutrophication of aquatic ecosystems, the recipients of municipal wastewater. These results are significant because there is not enough data concerning this topic in the temperate continental climate of Southeast Europe. In addition to this, Constructed Wetland System is the first system of this kind, developed in our country that has been functioning for past 18 years. Due to this, the results are encouraging the application of CWS for a large number of smaller settlements in Southeast Europe as well as in other similar areas. Also, the results obtained in this paper can be useful to all those who are committed to the environmental approach and are engaged in research related to the use of reed in the process of municipal wastewater treatment.
Assuntos
Nitrogênio , Áreas Alagadas , Biomassa , Nitrogênio/análise , Fósforo , Eliminação de Resíduos Líquidos/métodos , Biodegradação Ambiental , PoaceaeRESUMO
Symbiotic nitrogen fixation between legumes and rhizobia involves a coordinated expression of many plant and bacterial genes as well as finely tuned metabolic activities of micro- and macrosymbionts. In spite of such complex interactions, symbiotic proficiency remains a resilient process, with host plants apparently capable of compensating for some deficiencies in rhizobia. What controls nodule homeostasis is still poorly understood and probably varies between plant species. In this respect, the promiscuous Sinorhizobium (Ensifer) fredii strain NGR234 has become a model to assess the relative contribution of single gene products to many symbioses. Here, we describe how a deletion in nifQ of NGR234 (strain NGRΔnifQ) makes nodules of Vigna unguiculata, V. radiata, and Macroptilium atropurpureum but not of the mimisoid tree Leucaena leucocephala, purple-red. This peculiar dark-nodule phenotype did not necessarily correlate with a decreased proficiency of NGRΔnifQ but coincided with a 20-fold or more accumulation of coproporphyrin III and uroporphyrin III in V. unguiculata nodules. Porphyrin accumulation was not restricted to plant cells infected with bacteroids but also extended to the nodule cortex. Nodule metal-homeostasis was altered but not sufficiently to prevent assembly and functioning of nitrogenase. Although the role of NifQ in donating molybdenum during assembly of nitrogenase cofactor FeMo-co makes it essential in free-living diazotrophs, our results highlight the dispensability of NifQ in many legume species.
Assuntos
Proteínas de Bactérias , Metais , Nitrogênio , Porfirinas , Simbiose , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Homeostase , Metais/metabolismo , Nitrogênio/metabolismo , Fixação de Nitrogênio , Porfirinas/metabolismoRESUMO
Environmental pollution is an emerging global issue. Heterogenous photocatalytic degradation, which belongs to the advanced oxidation processes, is a promising sustainable technique for the removal of harmful pollutants (e.g., pharmaceuticals) from natural resources (surface and underground waters), as well as wastewaters. In our study, we examined the efficiency of photocatalytic degradation (with TiO2 and ZnO as photocatalysts) of tolperisone hydrochloride (TLP) and the effect of TLP and its degradation intermediates on germination, photosynthetic capacity, and biomass production of wheat. According to the UFLC-DAD and LC-ESI-MS results, we found that the complete degradation of TLP can be reached after 60.83 min of UV irradiation using TiO2 as a photocatalyst. Furthermore, we determined that germination, biomass production, and chlorophyll b (Chl b) were not related to the percentage of TLP after irradiation. Chlorophyll a (Chl a) (r = -0.61, p ≤ 0.05), Chl a+b (r = -0.56, p ≤ 0.05), and carotenoid (car) (r = -0.57, p ≤ 0.05) were strongly inversely (negatively) correlated with TLP, while Chl a+b/car (r = 0.36, p ≤ 0.05) was moderately (positively) related.
RESUMO
Imidazolium-based ionic liquids (ILs) have unique and tunable features with high potential in industrial use. However, the utilization of the ILs in industrial processes has recently arisen the question of their disposal and the effect on the environment. Therefore, in the present study, we investigated the effect of two commercial imidazolium-based ILs, 1-butyl-3-methylimidazolium chloride ([Bmim][Cl]) and 1-decyl-3-methylimidazolium chloride ([Dmim][Cl]) on the growth and chemical composition of widely grown vegetables - tomato and cucumber. Different concentrations (10, 100 or 1000 mg L-1) of [Bmim][Cl] and [Dmim][Cl] were applied to the soil on which tomato was cultivated. After the harvest of tomato fruits, the same soil was used to grow and analyze the growth and chemical composition of cucumber. ILs significantly reduced shoot biomass and yield of tomato and significantly changed concentrations of N, K, Ca, Fe and Mn in the leaves, whereas concentrations of P, Cu and Zn were at the level of respected controls. The number of fruits of cucumber, grown on the soil previously treated with ILs, was significantly reduced along with yield, and mineral composition of leaves was significantly altered, with the exception to Cu. [Dmim][Cl] in general affected both tomato and cucumber more than [Bmim][Cl]. The application of IL with a longer alkyl substituent ([Dmim][Cl]) increased the temperature inside the tomato canopy and accelerated the senescence of plants.
Assuntos
Cucumis sativus , Líquidos Iônicos , Solanum lycopersicum , Minerais , Folhas de PlantaRESUMO
The presence of excessive salts in the soil and irrigation water can change the growth and affect the metabolic functions of plants. Coriander (Coriandrum sativum L.) is often cultivated in arid and semiarid areas where irrigation with low-quality water and uneven distribution of rainfall may contribute to the accumulation of salts in the substrate. In the present study, coriander plants were cultivated in half-strength Hoagland nutrient solution containing 0.2, 0.6 and 1.2 g NaCl L-1 to assess the effect of low concentrations of NaCl on biomass production and mineral nutrients accumulation (Ca, K, Mg, Na, P). The presence of 0.2, 0.6 and 1.2 g NaCl L-1 slightly stimulated biomass production. The concentration of Na increased in coriander tissues (up to 4 times relative to control). However, concentration of K and Ca in leaves of plants treated with 1.2 g NaCl L-1 was decreased with respect to control. Based on the findings, even though biomass of coriander, under applied NaCl concentrations, was slightly increased, the chemical composition of its vegetative organs was severely disrupted by present salt which is very important for the quality of coriander as a popular herb or spice plant.
Assuntos
Biomassa , Coriandrum/crescimento & desenvolvimento , Minerais/análise , Cloreto de Sódio/efeitos adversos , Coriandrum/fisiologia , Cloreto de Sódio/metabolismoRESUMO
Pharmaceuticals and pesticides are emerging contaminants problematic in the aquatic environment because of their adverse effects on aquatic life and humans. In order to remove them from water, photocatalysis is one of the most modern technologies to be used. First, newly synthesized photocatalysts were successfully prepared using a sol-gel method and characterized by different techniques (XRD, FTIR, UV/Vis, BET and SEM/EDX). The photocatalytic properties of TiO2, ZnO and MgO nanoparticles were examined according to their removal from water for two antibiotics (ciprofloxacin and ceftriaxone) and two herbicides (tembotrione and fluroxypyr) exposed to UV/simulated sunlight (SS). TiO2 proved to be the most efficient nanopowder under UV and SS. Addition of (NH4)2S2O8 led to the faster removal of both antibiotics and herbicide fluroxypyr. The main intermediates were separated and identified for the herbicides and antibiotic ciprofloxacin. Finally, the toxicity of each emerging pollutant mixture and formed intermediates was assessed on wheat germination and biomass production.
RESUMO
The increasing application of various surfactants nowadays, may lead to the contamination of the natural environment and represent potential threat to terrestrial higher plants. In this article, the effect of 13 surfactants, with dodecyl alkyl chain and various aromatic (imidazolium, pyridinium, thiazolium) and aliphatic (guanidinium, ammonium, thiosemicarbazidium) polar heads, on germination, development and growth of wheat and cucumber was investigated. The study aimed to prove how changes in lipophilicity of surfactants and their various structural modifications (existence of the aliphatic or aromatic polar group, the introduction of oxygen and sulfur) influence toxicity towards investigated plants. The calculated lipophilic parameter (AlogP) is shown to be a useful parameter for predicting potential toxicity of the compound. The strategy of using surfactants with aliphatic polar heads instead of aromatic prove to be a promising strategy in reducing harmful effect, as well as the introduction of polar groups in the structure of cation. From all investigated compounds, surfactants with imidazolium polar head displayed the most harmful effect towards wheat and cucumber. The cucumber seeds were more sensitive to the addition of surfactants comparing to wheat. All obtained experimental results were additionally investigated using computational methods, simulating the transport of surfactants through a lipid bilayer. The influence of cation tendency to fit in lipid bilayer structure was correlated with toxicity. For the first time, it is concluded that cation ability to mimic the structure of bilayer have less harmful effect on plant development.
Assuntos
Cucumis sativus/efeitos dos fármacos , Imidazóis/toxicidade , Compostos de Piridínio/toxicidade , Tensoativos/toxicidade , Triticum/efeitos dos fármacos , Cátions , Membrana Celular/química , Membrana Celular/efeitos dos fármacos , Cucumis sativus/crescimento & desenvolvimento , Germinação/efeitos dos fármacos , Imidazóis/química , Bicamadas Lipídicas/química , Simulação de Dinâmica Molecular , Compostos de Piridínio/química , Compostos de Piridínio/farmacologia , Plântula/efeitos dos fármacos , Plântula/crescimento & desenvolvimento , Sementes/efeitos dos fármacos , Sementes/crescimento & desenvolvimento , Relação Estrutura-Atividade , Tensoativos/química , Triticum/crescimento & desenvolvimentoRESUMO
In this work, the effect on cucumber growth of seven different imidazolium-based ionic liquids, namely 1-(2-oxybutyl)-3-methylimidazolium chloride, [C2OC2mIm][Cl]; 1-(2-oxypropyl)-3-methylimidazolium chloride, [C1OC2mIm][Cl]; 1-(3-hydroxypropyl)-3-ethylimidazolium chloride, [OHC3eIm][Cl]; 1-(3-hydroxypropyl)-3-methylimidazolium chloride, [OHC3mIm][Cl]; 1-(2-hydroxyethyl)-3-methylimidazolium chloride, [OHC2mIm][Cl], 1-butyl-3-methylimidazolium chloride, [bmim][Cl] and imidazolium chloride, [Im][Cl], were examined. The influence of polarity of the alkyl side chain of the imidazolium cation on the reduction of the ionic liquid's toxicity is investigated. For all investigated seedlings, significant reduction of biomass was noted, with the incoherent influence of the ionic liquid (IL) concentration. The total inhibition of germination was shown at the highest used concentration for some of the used ionic liquids. Although investigated ILs affected root and shoot growth of cucumber, the effect on stress marker (MDA) as well as biosynthesis of chlorophyll and carotenoids was negligible. The data collected in this research suggest that tuning of the lipophilicity of imidazolium cations by the introduction of polar groups in the side alkyl chain does not have pronounced effect on cucumber, as it was shown for other plant species.