RESUMO
Triple negative breast cancer (TNBC) is the breast cancer subtype with the worst prognosis and still lacks a targeted therapy. In this study, we found increased ERK phosphorylation in TNBC cell lines and an important role for ERK in sustaining the migration of TNBC cells. Although ROS have been suggested to have an important role in sustaining MAPK signaling, antioxidant treatment increased ERK phosphorylation, probably suggesting increased invasive potential. Interestingly, treatment with PD0325901 (PD), a MEK inhibitor, decreased ROS levels in TNBC cells and decreased mitochondrial fragmentation in the MDAMB231 cell line. Our data supports an important role for MEK/ERK in TNBC, sustaining cellular migration, regulating mitochondrial dynamics and ROS production in this breast cancer subtype.
Assuntos
Neoplasias de Mama Triplo Negativas , Humanos , Neoplasias de Mama Triplo Negativas/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Quinases de Proteína Quinase Ativadas por Mitógeno , Proliferação de CélulasRESUMO
Trauma is the leading cause of pediatric morbidity and mortality in the United States. There are known gaps in access to pediatric trauma care and rehabilitation services. We postulate that aftercare is fragmented or nonexistent. We propose that postdischarge "medical home" style care, championed by a pediatric nurse practitioner, leads to improved short-term outcomes and caretaker and provider satisfaction.