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1.
Nat Immunol ; 9(11): 1261-9, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18820681

RESUMO

Succinate acts as an extracellular mediator signaling through the G protein-coupled receptor GPR91. Here we show that dendritic cells had high expression of GPR91. In these cells, succinate triggered intracellular calcium mobilization, induced migratory responses and acted in synergy with Toll-like receptor ligands for the production of proinflammatory cytokines. Succinate also enhanced antigen-specific activation of human and mouse helper T cells. GPR91-deficient mice had less migration of Langerhans cells to draining lymph nodes and impaired tetanus toxoid-specific recall T cell responses. Furthermore, GPR91-deficient allografts elicited weaker transplant rejection than did the corresponding grafts from wild-type mice. Our results suggest that the succinate receptor GPR91 is involved in sensing immunological danger, which establishes a link between immunity and a metabolite of cellular respiration.


Assuntos
Células Dendríticas/imunologia , Receptores Acoplados a Proteínas G/agonistas , Receptores Acoplados a Proteínas G/imunologia , Ácido Succínico/metabolismo , Linfócitos T/imunologia , Animais , Linhagem Celular Tumoral , Movimento Celular , Citocinas/biossíntese , Células Dendríticas/metabolismo , Rejeição de Enxerto/imunologia , Humanos , Células de Langerhans/citologia , Linfonodos/imunologia , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos SCID , Receptores de Antígenos de Linfócitos T/agonistas , Receptores de Antígenos de Linfócitos T/antagonistas & inibidores , Receptores de Antígenos de Linfócitos T/imunologia , Receptores Acoplados a Proteínas G/genética , Transdução de Sinais/imunologia , Ácido Succínico/farmacologia , Linfócitos T Auxiliares-Indutores/imunologia , Regulação para Cima
2.
Biochem Biophys Res Commun ; 369(3): 868-72, 2008 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-18328805

RESUMO

Based on the oxidation hypothesis high doses of alpha-tocopherol have been advocated to prevent atherosclerosis, but clinical trials failed to demonstrate a benefit. As specific oxylipids activate PPARgamma and LXRalpha, master regulators of lipid metabolism and cholesterol exporters, we hypothesized, that high dose alpha-tocopherol might interfere with reverse cholesterol transport out of the vessel wall. Human THP-1 cells, a foam cell model, were preincubated with alpha-tocopherol or carrier before exposure to oxidized LDL, delipidated HDL or control buffer. Specific mRNAs were quantified by real-time RT-PCR, LXRalpha activation by a reporter gene assay and cellular cholesterol homeostasis by oxLDL and dHDL facilitated uptake and efflux assays. alpha-Tocopherol significantly reduced baseline expression and stimulation by oxLDL of LXRalpha activity, CD36, ABCA1, and ABCG1. alpha-Tocopherol also reversed the suppression of CD36 and ABCA1 by dHDL. Thus alpha-Tocopherol compromises cellular lipid scavenging and channelling of cholesterol into reverse transport out of the vessel wall.


Assuntos
Transportadores de Cassetes de Ligação de ATP/antagonistas & inibidores , Antioxidantes/farmacologia , Colesterol/metabolismo , Proteínas de Ligação a DNA/antagonistas & inibidores , Células Espumosas/efeitos dos fármacos , Receptores Citoplasmáticos e Nucleares/antagonistas & inibidores , alfa-Tocoferol/farmacologia , Transportador 1 de Cassete de Ligação de ATP , Membro 1 da Subfamília G de Transportadores de Cassetes de Ligação de ATP , Aterosclerose/etiologia , Aterosclerose/prevenção & controle , Antígenos CD36/metabolismo , HDL-Colesterol/farmacologia , Proteínas de Ligação a DNA/agonistas , Proteínas de Ligação a DNA/metabolismo , Células Espumosas/metabolismo , Genes Reporter/efeitos dos fármacos , Humanos , Lipoproteínas LDL/farmacologia , Receptores X do Fígado , Receptores Nucleares Órfãos , Receptores Citoplasmáticos e Nucleares/agonistas , Receptores Citoplasmáticos e Nucleares/metabolismo , Transcrição Gênica/efeitos dos fármacos
3.
Cardiovasc Res ; 69(2): 527-35, 2006 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-16336952

RESUMO

OBJECTIVE: Marked anti-atheromatous effects of the anti-inflammatory cytokine interleukin-10 (IL-10) were observed in several lipid-driven animal models of arteriosclerosis. We therefore investigated whether IL-10 affects macrophage cholesterol handling. METHODS: Human THP-1 cells and peripheral monocytes served as macrophage models. Specific mRNA was quantified by real-time RT-PCR, protein expression by flow cytometry and Western blotting. Cellular cholesterol handling was studied by lipoprotein-facilitated uptake and efflux assays. IL-10 effects were also studied in cells transfected with liver X receptor alpha (LXRalpha)-siRNA or a LXRalpha response element (LXRE) reporter construct. RESULTS: Picomolar IL-10 suppressed basal and peroxisome proliferator-activated receptor gamma (PPARgamma)-stimulated transcription of the scavenger receptor CD36 due to reduced PPARgamma protein expression. In contrast, IL-10 stimulated transcription of the active cellular cholesterol exporters ATP-binding cassette transporters A1 and G1 (ABCA1, ABCG1) and the LDL receptor, whereas scavenger receptor-BI (SR-BI) was unchanged. The reduction of CD36 and stimulation of ABCA1 expression was confirmed in human monocytes. Thereby, IL-10 prevented cellular cholesterol overloading from oxidized LDL (oxLDL) and enhanced efflux to apoA-containing particles initiating reverse cholesterol transport. Experiments with inhibitors, LXRalpha silencing and the LXRE reporter gene construct supported the proximal transmission of the IL-10 effect on ABCA1 by the IL-10 receptor/signal transducer and activator of transcription 3 (STAT3) pathway and distal cross-talk to the LXRalpha and PPARalpha/retinoic acid X receptor (RXR) and cAMP/protein kinase A (PKA) pathways. CONCLUSIONS: In addition to immune and anti-inflammatory actions, IL-10 redirects macrophage cholesterol handling towards reverse cholesterol transport, which contributes to its anti-atherosclerotic action.


Assuntos
Aterosclerose/imunologia , Interleucina-10/fisiologia , Lipoproteínas LDL/metabolismo , Macrófagos/metabolismo , Receptores de Complemento 3b/metabolismo , Transportador 1 de Cassete de Ligação de ATP , Transportadores de Cassetes de Ligação de ATP/genética , Transportadores de Cassetes de Ligação de ATP/metabolismo , Aterosclerose/metabolismo , Western Blotting/métodos , Linhagem Celular , Colesterol/metabolismo , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Citometria de Fluxo , Humanos , Receptores X do Fígado , Receptores Nucleares Órfãos , PPAR gama/metabolismo , RNA Interferente Pequeno/genética , Receptores Citoplasmáticos e Nucleares/genética , Receptores Citoplasmáticos e Nucleares/metabolismo , Transdução de Sinais/fisiologia , Transfecção
4.
Arterioscler Thromb Vasc Biol ; 25(3): 546-52, 2005 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-15618544

RESUMO

OBJECTIVES: Sphingosine 1-phosphate (S1P) is a bioactive phospholipid acting both as a ligand for the G protein-coupled receptors S1P1-5 and as a second messenger. Because S1P1 knockout is lethal in the transgenic mouse, an alternative approach to study the function of S1P1 in endothelial cells is needed. METHODS AND RESULTS: All human endothelial cells analyzed expressed abundant S1P1 transcripts. We permanently silenced (by RNA interference) the expression of S1P1 in the human endothelial cell lines AS-M.5 and ISO-HAS.1. The S1P1 knock-down cells manifested a distinct morphology and showed neither actin ruffles in response to S1P nor an angiogenic reaction. In addition, these cells were more sensitive to oxidant stress-mediated injury. New S1P1-dependent gene targets were identified in human endothelial cells. S1P1 silencing decreased the expression of platelet-endothelial cell adhesion molecule-1 and VE-cadherin and abolished the induction of E-selectin after cell stimulation with lipopolysaccharide or tumor necrosis factor-alpha. Microarray analysis revealed downregulation of further endothelial specific transcripts after S1P1 silencing. CONCLUSIONS: Long-term silencing of S1P1 enabled us for the first time to demonstrate the involvement of S1P1 in key functions of endothelial cells and to identify new S1P1-dependent gene targets.


Assuntos
Endotélio Vascular/fisiologia , Biologia Molecular/métodos , RNA Interferente Pequeno/genética , Receptores de Lisoesfingolipídeo/genética , Receptores de Lisoesfingolipídeo/metabolismo , Antígenos CD , Caderinas/genética , Linhagem Celular , Endotélio Vascular/citologia , Inativação Gênica , Hemangiossarcoma , Humanos , Microcirculação , Neovascularização Fisiológica/fisiologia , Análise de Sequência com Séries de Oligonucleotídeos , Estresse Oxidativo/fisiologia , Molécula-1 de Adesão Celular Endotelial a Plaquetas/genética , Circulação Pulmonar , RNA Mensageiro/genética , Sistemas do Segundo Mensageiro/fisiologia , Células Tumorais Cultivadas , Fator de Necrose Tumoral alfa/farmacologia , Veias Umbilicais/citologia , Neoplasias Vasculares , Vasculite/genética , Vasculite/imunologia , Vasculite/fisiopatologia
5.
Biochem Pharmacol ; 67(3): 411-9, 2004 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-15037193

RESUMO

Niacin, the first lipid lowering drug shown to improve survival after myocardial infarction, decreases LDL and increases HDL cholesterol levels. These effects cannot fully be explained by its suspected mechanism of action, inhibition of lipolysis and hepatic VLDL synthesis. Niacin has also been shown to interfere with the cyclic AMP (cAMP)/protein kinase A (PKA) pathway and massively stimulate prostaglandin D2 (PGD2) formation. The major metabolite of PGD2, 15-deoxy-Delta(12,14)-prostaglandin J2 (15d-PGJ2), was recently identified as the most potent endogenous PPARgamma activator. We, therefore, studied the effects of niacin on the PPARgamma- and cAMP-dependent expression of receptors promoting reverse cholesterol transport. The transcription of PPARgamma-, HDL-, LDL- and scavenger-receptors and the sterol exporter ABCA1, were measured by quantitative RT-PCR and cellular cholesterol efflux and PPARgamma activation studied in macrophage and hepatocyte models. Niacin stimulated the translocation of PPARgamma and the transcription of PPARgamma, CD36 and ABCA1 in monocytoid cells, whereas the LDL-receptor (LDL-R) was unchanged. Thereby niacin enhanced HDL-mediated cholesterol efflux from the cells resulting in a reduced cellular cholesterol content. The niacin effect on CD36 but not on ABCA1 was prevented by cyclooxygenase inhibition, whereas the niacin effect on ABCA1 but not on CD36 was prevented by PKA inhibition, suggesting mediation by the 15d-PGJ2/PPARgamma and the cAMP/PKA pathways, respectively. These new actions of niacin on several key effectors of reverse cholesterol transport out of the vessel wall provide a rational to expect regression of atherosclerosis and test the combination of niacin with statins for an overadditive clinical benefit.


Assuntos
Transportadores de Cassetes de Ligação de ATP/metabolismo , Antígenos CD36/metabolismo , Colesterol/metabolismo , Hipolipemiantes/farmacologia , Macrófagos/efeitos dos fármacos , Niacina/farmacologia , Transportador 1 de Cassete de Ligação de ATP , Transporte Biológico/efeitos dos fármacos , Células Cultivadas , Humanos , Macrófagos/metabolismo
6.
Aging (Albany NY) ; 5(6): 427-44, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23833031

RESUMO

Age-related macular degeneration (AMD) is a prominent cause of blindness in the Western world. To date, its molecular pathogenesis as well as the sequence of events leading to retinal degeneration remain largely ill-defined. While the invasion of choroidal neovessels in the retina is the primary mechanism that precipitates loss of sight, an earlier dry form precedes it. Here we provide the first evidence for the protective role of the Retinal Pigment Epithelium (RPE)-resident metabolite receptor, succinate receptor 1 (SUCNR1; G-Protein coupled Receptor-91 (GPR91), in preventing dry AMD-like lesions of the outer retina. Genetic analysis of 925 patients with geographic atrophy and 1199 AMD-free peers revealed an increased risk of developing geographic atrophy associated with intronic variants in theSUCNR1 gene. In mice, outer retinal expression of SUCNR1 is observed in the RPE as well as microglial cells and decreases progressively with age. Accordingly, Sucnr1-/- mice show signs of premature sub-retinal dystrophy with accumulation of oxidized-LDL, abnormal thickening of Bruch's membrane and a buildup of subretinal microglia. The accumulation of microglia in Sucnr1-deficient mice is likely triggered by the inefficient clearance of oxidized lipids by the RPE as bone marrow transfer of wild-type microglia into Sucnr1-/- mice did not salvage the patho-phenotype and systemic lipolysis was equivalent between wild-type and control mice. Our findings suggest that deficiency in SUCNR1 is a possible contributing factor to the pathogenesis of dry AMD and thus broaden our understanding of this clinically unmet need.


Assuntos
Degeneração Macular/metabolismo , Receptores Acoplados a Proteínas G/metabolismo , Animais , Transplante de Medula Óssea , Lâmina Basilar da Corioide/metabolismo , Lâmina Basilar da Corioide/patologia , DNA/genética , Regulação da Expressão Gênica/fisiologia , Variação Genética , Humanos , Modelos Logísticos , Degeneração Macular/genética , Camundongos , Camundongos Knockout , Microglia/metabolismo , Receptores Acoplados a Proteínas G/genética , Retina/metabolismo
7.
Biochem Biophys Res Commun ; 290(3): 988-93, 2002 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-11798172

RESUMO

Modified low-density lipoprotein (LDL) has been implicated as an initiating or amplifying factor in atherogenesis. Some of its biological activities, such as apoptosis induction and upregulation of the scavenger receptor CD36, appear to share common signaling pathways in cells of the cardiovascular system. Exposure of low-differentiated monocytic cells to LDL modified with 15-lipoxygenase and secretory phospholipase A(2) induced apoptosis and upregulated CD36. Cell treatment with constituents of modified LDL, such as 13-hydroxyoctadecadienoic acid (13-HODE), 25-hydroxycholesterol, and lysophosphatidyl choline, and with an unrelated apoptogen (TNF-related apoptosis-inducing ligand) induced apoptosis. In contrast, only 13-HODE caused upregulation of CD36 expression. Cotreatment with the pan-caspase inhibitor z.VAD-fmk resulted in suppression of apoptosis, but was without any effect on CD36 expression. These data indicate that in monocytic cells enzymatically modified LDL is capable of inducing both apoptosis and upregulation of CD36 expression. However, in our cellular model, the two induction processes appear to be causally unrelated.


Assuntos
Apoptose , Antígenos CD36/biossíntese , Lipoproteínas LDL/metabolismo , Lipoproteínas LDL/farmacologia , Monócitos/metabolismo , Regulação para Cima , Araquidonato 15-Lipoxigenase/metabolismo , Linhagem Celular , Humanos , Ácidos Linoleicos/farmacologia , Monócitos/citologia , Monócitos/efeitos dos fármacos , Fosfolipases A/metabolismo , RNA Mensageiro/biossíntese , Ativação Transcricional
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