Requirement for Phe36 for DNA binding and mismatch repair by Escherichia coli MutS protein.

The MutS family of DNA repair proteins recognizes base pair mismatches and insertion/deletion mismatches and targets them for repair in a strand-specific manner. Photocrosslinking and mutational studies previously identified a highly conserved Phe residue at the N-terminus of Thermus aquaticus MutS protein that is critical for mismatch recognition in vitro. Here, a mutant Escherichia coli MutS protein harboring a substitution of Ala for the corresponding Phe36 residue is assessed for proficiency in mismatch repair in vivo and DNA binding and ATP hydrolysis in vitro. The F36A protein is unable to restore mismatch repair proficiency to a mutS strain as judged by mutation to rifampicin or reversion of a specific point mutation in lacZ. The F36A protein is also severely deficient for binding to heteroduplexes containing an unpaired thymidine or a G:T mismatch although its intrinsic ATPase activity and subunit oligomerization are very similar to that of the wild-type MutS protein. Thus, the F36A mutation appears to confer a defect specific for recognition of insertion/deletion and base pair mismatches.

What are women told about Pap smears that lack endocervical cells?

BACKGROUND: Debate exists about the definition of what constitutes an adequate Pap smear and about the recommended rescreening interval for Pap smears lacking an endocervical component. This study aimed at determining whether women are currently informed about the endocervical status of their Pap smears and what rescreening recommendations are made to women whose smears lack endocervical cells. METHOD: Consecutive Pap smears lacking an endocervical component were identified from pathology records. After obtaining consent from the referring doctor, 165 women were interviewed by telephone. RESULTS: Only 110 (67%) of 165 women received active notification of their Pap test result and only six (4%) were aware that their smear lacked endocervical cells. Thirteen (8%) had been advised to have a repeat smear within three months. Nearly half the women reported that they would like more information about their result. CONCLUSIONS: It seems that current Pap smear notification patterns for women in New South Wales could be improved. One third are not actively informed at all about their results, and few are given detailed information about their Pap test results. Methods of enhancing the level of information women are given about their medical and screening tests need to be improved.

Sexually transmitted infections and use of sexual health services among young Australian women: women's health Australia study.

Our objective was to examine associations between self-reported sexually transmitted infections (STIs) and sociodemographic, lifestyle, health status, health service use and quality of life factors among young Australian women; and their use of family planning and sexual health clinics and associations with health, demographic and psychosocial factors. The study sample comprised 14,762 women aged 18-23 years who participated in the mailed baseline survey for the Australian Longitudinal Study on Women's Health, conducted in 1996. The main outcome measures are self report of ever being diagnosed by a doctor with an STI, including chlamydia, genital herpes, genital warts or other STIs, and use of family planning and sexual health clinics. The self-reported incidence of STI was 1.7% for chlamydia, 1.1% genital herpes, 3.1% genital warts, and 2.1% other STIs. There was a large number of demographic, health behaviour, psychosocial and health service use factors significantly and independently associated with reports of having had each STI. Factors independently associated with use of family planning clinic included unemployment, current smoking, having had a Pap smear less than 2 years ago, not having ancillary health insurance, having consulted a hospital doctor and having higher stress and life events score. Factors independently associated with use of a sexual health clinic included younger age, lower occupation status, being a current or ex-smoker, being a binge drinker, having had a Pap smear, having consulted a hospital doctor, having poorer mental health and having higher life events score. This study reports interesting correlates of having an STI among young Australian women aged 18-23. The longitudinal nature of this study provides the opportunity to explore the long-term health and gynaecological outcomes of having STIs during young adulthood.

Effectiveness of two strategies for dissemination of sun-protection policy in New South Wales primary and secondary schools.

With rising rates of skin cancer in Australia, there is a need to examine strategies to reduce sun exposure among children. This study aimed to determine the effectiveness of a multifaceted dissemination strategy compared with a simple mail-out strategy in promoting the adoption of comprehensive SunSmart skin protection policies and practices in primary and secondary schools in New South Wales. It also aimed to examine characteristics of the primary and secondary schools that adopted a comprehensive SunSmart policy before and after the intervention. Four hundred randomly selected primary schools and all 381 high schools in New South Wales were randomised to one of two intervention groups. Pretest and post-test surveys of principals were undertaken in 1991 and 1992. Intervention 1 was a simple mail-out of a sample sun-protection policy kit. Intervention 2 comprised the mail-out of the policy kit and a follow-up mail-out of a staff development module. There was a strong intervention effect on adoption of a comprehensive sun-protection policy in primary schools (21 per cent for the 'mail' group compared with 44 per cent for 'mail and staff support' group) but not in high schools (6 per cent and 11 per cent). There was little relationship between adoption of a comprehensive sun-protection policy and sun-protection practices in primary or secondary schools. Further research is needed to determine the most effective ways of ensuring that adoption of a comprehensive sun-protection policy results in effective implementation of sun-protection practices in schools.

Cigarette smoking, menstrual symptoms and miscarriage among young women.

OBJECTIVE: To examine associations between cigarette smoking and menstrual symptoms and miscarriage among young women. METHOD: The study sample consists of 14,779 women aged 18-23 years who participated in the mailed baseline survey for the Australian Longitudinal Study on Women's Health, conducted in 1996. The main outcome measures are self reported menstrual symptoms and miscarriages. RESULTS: Current smokers and ex-smokers had an increased risk of menstrual symptoms and miscarriages compared with women who had never smoked, with the highest risk occurring in heavy smokers (adjusted odds ratios for those smoking > or = 20 cigarettes per day: premenstrual tension 1.5 (95% confidence interval 1.3 to 1.7), irregular periods 1.5 (1.3 to 1.8), heavy periods 1.6 (1.4 to 1.9), severe period pain 1.5 (1.4 to 1.7), one or more miscarriages 2.0 (1.5 to 2.8). The odds ratios generally increased with numbers of cigarettes smoked and a younger age of starting to smoke. CONCLUSION: This study suggests that young women who smoke are at higher risk of a range of menstrual problems and miscarriage than those who have never smoked. The immediacy of this risk (in contrast to the longer term risks of chronic disease) can be used to improve the relevance of anti-smoking campaigns targeted to young women.

Interventions with retailers to reduce cigarette sales to minors: a randomised controlled trial.

We aimed to determine the relative effectiveness of an education intervention and a threat-of-enforcement intervention in reducing sales of cigarettes to under-age youth by randomly allocating 300 retailers in a nonmetropolitan region of New South Wales to: a control group with no intervention; a minimal-intervention group, which received an educational letter; and a maximal-intervention group, which received a threat of enforcement followed by a visit from a public health officer. Retailers were checked for compliance at pretest and post-test, six months apart, by twelve 18-year-olds who were judged by independent raters to look younger. The retailers were surveyed by telephone at both times for knowledge, attitudes and self-reported sales practices. Neither intervention achieved significant improvements for the two key behavioural outcomes: requiring proof of age and display of a warning sign. Neither was there an intervention effect on knowledge about the law. The greatest improvement in the proportion of retailers who believed that the legal age should be 18 or over was in the minimal-intervention group, and both intervention groups were less likely than the control group at post-test to think that it was acceptable to sell to a person who was nearly 18. There was poor overall compliance with the revised legislation at pre-test. The finding of a pretest-to-post-test improvement but no differential intervention effect highlights the methodological difficulties of such research. The interventions may, however, have been partly successful in modifying the attitudes of retailers.

Validity of the SF-12 Compared with the SF-36 Health Survey in Pilot Studies of the Australian Longitudinal Study on Women's Health.

This study assessed the relative validity of the Medical Outcomes Study Short-Form General Health Survey (SF-36 and SF-12) for Australian women and compared norms for the independently administered and embedded SF- 12. Two samples of women in New South Wales were randomly selected from the Medicare database ( N = 3600). The sample was stratified into young (18 to 22 years), middle-aged (45 to 49 years) and older (70 to 74 years) women, and into women living in urban (40 percent), rural (30 percent) and remote (30 percent) areas. In study 3500 households were selected by random digit dial. The method was a mailout survey. SF-36 scale scores were similar to US norms. For the older group, health profiles of the independent and embedded SF- 12 differed. For the SF-36 and independently administered SF- 12, means differed in all three age groups. The SF-12 physical health scores discriminated between women with poor versus good physical health, and mental health scores discriminated between groups who were or were not psychologically distressed on GHQ-12. The SF- 36, relative to the SF-12, appears to be a more reliable measure for examining changes in health status over time and between groups.

SF-36 Health Profiles of Recently Discharged Hospital Patients in Australia.

This study investigated the characteristics and validity of the SF-36 (Australian version) health profile and summary scores for recently discharged hospital patients and compared this with US and Australian community samples with minor and serious medical conditions. Adult medical, surgical, obstetric and gynaecological patients from a large teaching hospital in NSW, Australia, completed a postal survey 1 week after discharge. The SF- 36 discriminated well among patient groups and performed well on most validity tests. Scale and summary scores varied by age, gender, seriousness of condition on admission, prior health status and hospital group. The findings highlight the potential of the SF-36 in monitoring the health status of Australian hospital patients and assessing the health-related support needed on discharge.

Patient-provider agreement on guidelines for preparation for breast cancer treatment.

Guidelines for preparing cancer patients for threatening medical procedures were developed and refined and their perceived relevance and importance rated by three concerned groups--84 breast cancer patients, 64 doctors, and 140 nurses and nurse oncologists. All three groups indicated strong support for the guidelines. Patients and nurses rated more of the guidelines as essential aspects of good quality care than did doctors. Items in which a significant discrepancy existed included the importance of (a) consistent information, (b) involvement of others in preparation, and (c) assistance to the patient in coping with treatment for breast cancer. Doctors, compared with patients and nurses, underrated the importance of some aspects of preparation. These issues should be given more prominence in undergraduate and specialist medical training, as well as in continuing medical education.

Is there consensus between breast cancer patients and providers on guidelines for breaking bad news?

Eighty-four breast cancer patients, 64 oncologists, and 140 oncology nurses rated the importance of 15 general principles and 12 recommended steps to guide clinicians in breaking bad news to patients. At least 70% of the three samples rated 7 of the 15 principles and 6 of the 12 steps as essential. All three groups agreed that patients have a legal and moral right to accurate and reliable information and that patients should be given the diagnosis and prognosis honestly and in simple language, though not bluntly. The groups differed on the relative importance of other items, with less variation about the steps than about the principles. Patients' perceptions of the importance of various guideline steps and principles are probably most important, given that patients receive the troubling news and that research indicates that how the news is delivered is associated with important patient outcomes. Recommendations for further research are discussed.

Lessons learned from the Hunter Region Health Promoting Schools Project in New South Wales, Australia.

This paper describes development, over a five-year period, of the Hunter Region Health Promoting Schools Project in New South Wales (NSW), Australia. The project implemented the principles and philosophy of the Health Promoting Schools concept and evaluated its effectiveness using a randomized controlled trial involving 22 public secondary schools. An overview of the preliminary intervention model based on the health promoting schools philosophy and trialed in a pilot study is provided. The authors also outline barriers to, and difficulties in, implementing the philosophically based intervention model in the secondary school setting. The current intervention approach, which evolved over five years, is described in relation to the roles played by project team members and school communities. In addition, the authors outline guiding principles arising from the new approach which facilitated adoption of health promotion strategies in secondary school settings.

How to prepare cancer patients for potentially threatening medical procedures: consensus guidelines. NSW Cancer Council Cancer Education Research Program.

BACKGROUND: Cancer patients face a frightening array of potentially threatening medical interventions. While the need to prepare patients for such procedures is widely acknowledged, there have been few attempts to clearly specify in detail what adequate preparation entails. METHODS: This study sought to develop a set of guidelines on how to prepare cancer patients for potentially threatening medical procedures. These guidelines were extensively reviewed and modified by a consensus panel of experts. The guidelines were then rated by 101 cancer patients. RESULTS AND CONCLUSIONS: These ratings led to further modification of the guidelines, the final version of which was developed into a training module for junior medical officers in New South Wales.

Community attitudes to bans on smoking in licensed premises.

Information about public attitudes to the restriction of smoking in licensed premises could provide an impetus for a campaign to address the failure of the industry to improve the health of its employees and the public. A probability sample of 816 people was surveyed to determine community attitudes to the introduction of bans on smoking in licensed premises. A total ban on smoking was supported by 20 per cent and provision of special smoking areas by 65 per cent, and 15 per cent wanted no bans. More-educated people, white-collar workers, nonsmokers and those who went to licensed premises less than weekly were more likely to support bans than were the less educated, blue-collar workers, smokers, and those who went to licensed premises at least weekly. The less educated, smokers and those who went to licensed premises at least weekly were most likely to perceive that the introduction of smoking bands would reduce their patronage of licensed premises. Nonsmokers and those with more than 12 years of education were more likely to report that their patronage would increase if bands were introduced than were smokers and the less educated. The introduction of bans on smoking in licensed premises would result in only a small loss of patronage after accounting for potential increases from supporters of bans. The effect of the bans is likely to be felt most strongly among the less educated, smokers and regular patrons.

Norms for the physical and mental health component summary scores of the SF-36 for young, middle-aged and older Australian women.

The SF-36 was developed in the USA to provide an eight-scale health profile and two component summary scores representing physical and mental health. The published norms and scoring procedures are based on data from the US general population. The Australian Longitudinal Study on Women's Health (Women's Health Australia) undertook a survey in 1996 of approximately 42,000 Australian women in three age groups of 18-22, 45-49 and 70-74 years and provided age- and gender-specific norms for the SF-36 health profile. From these data, factor weights and factor score coefficients were calculated for these age- and gender-specific populations of Australian women. Thus, component summary scores for physical and mental health can now be calculated using a formula standardized to the relevant Australian population. This will facilitate the interpretation of the physical and mental health component summary scores in the Australian context and will allow meaningful comparisons within the young, middle-aged and older cohorts of Australian women in the Australian Longitudinal Study on Women's Health.

Smoking on hospital grounds and the impact of outdoor smoke-free zones.

OBJECTIVES: To describe the type and location of smokers on the grounds of smoke-free public hospitals and to observe the impact of introducing smoke-free signs in outdoor areas of the hospital grounds. DESIGN: Observation study of outdoor smoking behaviour before and after the introduction of outdoor smoke-free signs at one hospital (H1) and at the same two time periods at a nearby control hospital (H2), which already had some outdoor smoke-free signs at Time 1. SETTING: The John Hunter Hospital (H1) in Newcastle, Australia and a nearby control hospital, Maitland Hospital (H2) in 1991. SUBJECTS: All people in defined outdoor sites of the two hospitals on seven randomly selected days over two weeks before and after the policy change were coded as either "smoker" or "non-smoker" and as either "staff", "patient", or "visitor". The number of smokers observed in each site was measured as a proportion of all smokers observed on the grounds of that hospital. INTERVENTION: Introduction of outdoor smoke-free zones and signs at H1. RESULTS: Less than 10% of observed outdoor smokers in both hospitals were patients, 40% were visitors, and more than 50% were staff. Of outdoor smokers, 82% were observed less than 10 m from entrances to the hospital building at time 1. After the introduction of signs in H1, a 4-percentage point decrease (P < 0.05) occurred in the percentage of smokers observed in smoke-free zones at time 2 (from 32% to 28%), compared with a 2-percentage point decrease (P > 0.05 at time 2 in H2 (from 48% to 46%). DISCUSSION: This observation study of smoking behaviour in hospital grounds highlights the need to reduce smoking among staff and visitors near hospital entrances. Specific strategies are discussed, which are likely to enhance compliance and hence enable the effective introduction of smoke-free policies on hospital grounds.

'It would be good to know you're not alone': the health care needs of women with menstrual symptoms.

OBJECTIVES: We aimed to explore how menstrual symptoms affect women, women's health care needs, and their expectations and experiences when seeking care; to identify ways to assist women in having their needs met. METHODS: Qualitative research using focus group methodology was carried out. Identification of women experiencing menstrual symptoms through a random community survey of 200 women aged 30-50 years in the Hunter region of New South Wales. Focus group discussions were recorded and transcripts were analysed. RESULTS: All women attending focus groups had sought medical advice for their menstrual problems. Having one doctor with whom they felt comfortable was important. Women expressed difficulties asking questions and were concerned that symptoms may not be taken seriously or may be dismissed as psychological. There was widespread acceptance of alternative 'natural' therapies. CONCLUSIONS: Interactions between doctors (particularly GPs) and women with menstrual symptoms are central to how women perceive the care they receive. There is a need for doctors to demonstrate empathy. For many women, what they needed most from their doctors was to be understood and 'to know they weren't alone'.

Patterns and correlates of hormone replacement therapy use among middle-aged Australian women.

In this study, we examine the patterns of use of hormone replacement therapy (HRT) among women age 51 to 60 years and describe the characteristics of women who currently use HRT, previously used HRT, and have never used HRT. A brief postal survey of 800 women in this age range was used to determine HRT status. Telephone interviews were then conducted with 258 women (111 currently using HRT, 47 who previously used HRT, and 100 who had never used HRT) to determine characteristics of women who currently or previously used HRT or never used HRT, type of HRT used, duration of use, and reasons for use and nonuse. Nearly 40% of women were currently using HRT, 14% had previously used HRT, and 47% had never used HRT. Women currently using HRT were more likely than those not using HRT to have had a hysterectomy, attribute a greater number of symptoms to the climacteric, be in paid employment, and report a greater number of visits to the doctor over the past 12 months. HRT use among Australian women in their 50s is high and rising. Hysterectomy status, the attribution of symptoms to menopause, paid employment, and health care use were the most important correlates of HRT use. Few women specified long-term prevention of osteoporosis or heart disease as a reason for taking HRT.

Alcohol sales to minors: a surrogate study.

BACKGROUND: Legislation is a potentially important health promotion strategy to control the consumption of tobacco and alcohol by minors. Effectiveness of such laws may depend on the extent of which laws are adequately drafted, implemented, and enforced. However, studies of adherence with laws banning the sale of cigarettes to minors indicate low levels of enforcement. As a comparison, this study investigated adherence of alcohol retailers to legislation banning the sale of alcohol to minors in New South Wales, Australia. The aim was to determine the proportion and characteristics of alcohol retail outlets which sell alcohol to apparently underage youth. METHOD: Eight 18-year-olds who were judged by independent raters to look younger than the legal age attempted to purchase alcohol from 168 alcohol retail outlets (hotels, registered clubs, and bottle shops), in a nonmetropolitan region of New South Wales. RESULTS: No challenge about age was made for 68% of purchases. Youth were asked their age on 8% of occasions and proof of age was required on only 24% of occasions. Display of a warning sign was not associated with being required to show proof of age, nor was gender or dress of the buyer (casual clothes vs school uniform). Bottle shops were more likely to require proof of age than hotels or clubs. CONCLUSIONS: The results suggest that the implementation of legislation banning the sale of alcohol to minors is currently inadequate and that new efforts are needed to discourage the sale of alcohol to minors.

Availability of cigarettes to minors.

Legislation banning the sale of cigarettes to minors is potentially a cost-effective means of reducing smoking rates among adolescents. Such legislation has been in existence in Australia for over 80 years. Two studies examined the retail industry's adherence to the sales ban in two regions of New South Wales in 1990. The first study was a survey of 1,849 12- to 15-year-old adolescents from 12 high schools in New South Wales, providing data on self-reported purchasing of cigarettes from retail outlets by minors. In all, 38 per cent reported having purchased cigarettes illegally. A second study was undertaken to determine the proportion of retail shops which sell cigarettes to apparently underage youth. Two 16-year-olds who looked young for their age attempted to purchase cigarettes from 101 different retail outlets in one region of New South Wales. No challenge about age was made for 70 per cent of purchases, and proof of age was requested on only 15 per cent of occasions. The results suggest that legislation banning sales of cigarettes to minors requires strong enforcement procedures to be effective.

Responsiveness of the corpus luteum of the rhesus monkey (Macaca mulatta) to gonadotrophin in vitro during spontaneous and prostaglandin F2 alpha-induced luteolysis.

The ability of luteal tissue from rhesus monkeys, collected from spontaneous and prostaglandin F2 alpha (PGF2 alpha) induced luteolytic cycles, to secrete progesterone in response to hCG or dibutyryl cAMP in vitro was assessed. It was expected that the corpus luteum exposed to PGF2 alpha would behave in a similar manner to the corpus luteum of normal cycles undergoing luteal regression. PGF2 alpha (10 ng microliters-1 h-1) or vehicle (1 microliter h-1) was infused into the corpus luteum from 7 days after the preovulatory oestradiol surge. Lutectomy was performed at 2, 3 and 4 days after the start of the intraluteal infusion and at menses. Luteal progesterone content was determined and the secretion of progesterone in response to hCG (10 miu ml-1) or dibutyryl cAMP (5 mmol l-) was evaluated in vitro. A third group of monkeys underwent lutectomy sequentially during the luteal phase and were grouped by luteal age from days after the preovulatory oestradiol surge: early luteal (days 4-6), mid-luteal (days 7-9), late luteal (days 10-14) and menses. Immediately before luteal excision, an ovarian venous blood sample was taken for progesterone determination. Approximately 2-5 mg of minced luteal tissue was incubated at 37 degrees C in 2 ml of Krebs-Ringer bicarbonate buffer for 2 h in the presence or absence of hCG or dibutyryl cAMP.(ABSTRACT TRUNCATED AT 250 WORDS)