RESUMO
Vertebrate cells synthesize two forms of the 82- to 90-kilodalton heat shock protein that are encoded by distinct gene families. In HeLa cells, both proteins (hsp89 alpha and hsp89 beta) are abundant under normal growth conditions and are synthesized at increased rates in response to heat stress. Only the larger form, hsp89 alpha, is induced by the adenovirus E1A gene product (M. C. Simon, K. Kitchener, H. T. Kao, E. Hickey, L. Weber, R. Voellmy, N. Heintz, and J. R. Nevins, Mol. Cell. Biol. 7:2884-2890, 1987). We have isolated a human hsp89 alpha gene that shows complete sequence identity with heat- and E1A-inducible cDNA used as a hybridization probe. The 5'-flanking region contained overlapping and inverted consensus heat shock control elements that can confer heat-inducible expression on a beta-globin reporter gene. The gene contained 10 intervening sequences. The first intron was located adjacent to the translation start codon, an arrangement also found in the Drosophila hsp82 gene. The spliced mRNA sequence contained a single open reading frame encoding an 84,564-dalton polypeptide showing high homology with the hsp82 to hsp90 proteins of other organisms. The deduced hsp89 alpha protein sequence differed from the human hsp89 beta sequence reported elsewhere (N. F. Rebbe, J. Ware, R. M. Bertina, P. Modrich, and D. W. Stafford (Gene 53:235-245, 1987) in at least 99 out of the 732 amino acids. Transcription of the hsp89 alpha gene was induced by serum during normal cell growth, but expression did not appear to be restricted to a particular stage of the cell cycle. hsp89 alpha mRNA was considerably more stable than the mRNA encoding hsp70, which can account for the higher constitutive rate of hsp89 synthesis in unstressed cells.
Assuntos
Regulação da Expressão Gênica , Genes , Proteínas de Choque Térmico/genética , Proteínas Precoces de Adenovirus , Sequência de Aminoácidos , Sequência de Bases , Sangue , Southern Blotting , Linhagem Celular , Clonagem Molecular , DNA/genética , Humanos , Dados de Sequência Molecular , Peso Molecular , Família Multigênica , Proteínas Oncogênicas Virais/fisiologia , Regiões Promotoras Genéticas , RNA Mensageiro/genética , Mapeamento por Restrição , TransfecçãoRESUMO
Plasmids containing cDNA copies of mRNAs induced in HeLa cells by heat shock have been isolated and characterized. In vitro translation of RNAs selected by hybridization to plasmid DNAs identified sequences representing the three major classes (89, 70 and 27-kDa) of heat-shock proteins (hsp) and a 60-kDa minor hsp. Plasmids with inserts specific for the 27, 60, and 70-kDa hsp each hybridize with a single discrete size class of heat-inducible mRNA. Plasmids specific for the 89-kDa protein, however, hybridize with either a 2.7- or 2.95-kb mRNA species. Both mRNAs are coordinately induced during heat shock. We show that the characteristic pattern of induction and repression of each class of hsp during sustained hyperthermia is the result of changes in the steady state level of each mRNA.
Assuntos
Clonagem Molecular , DNA/metabolismo , Genes , Proteínas de Choque Térmico/genética , RNA Mensageiro/genética , Transcrição Gênica , Enzimas de Restrição do DNA , Células HeLa/metabolismo , Temperatura Alta , Humanos , Hibridização de Ácido Nucleico , Plasmídeos , Biossíntese de ProteínasRESUMO
An efficient method for the isolation of RNA from cartilage is described. The difficulties in obtaining RNA from cartilage, a tissue of low cell density and high proteoglycan content, were overcome by making several modifications to the guanidine thiocyanate/cesium chloride method of RNA extraction. Cartilage tissue is frozen, crushed, and homogenized in a 4 M guanidine thiocyanate lysis buffer. The RNA is then pelleted by ultracentrifugation through a cesium trifluoroacetate density gradient. The use of cesium trifluoroacetate, rather than cesium chloride, for density gradient centrifugation improves both the yield and purity of total RNA isolated from cartilage. The ultracentrifugation has been adapted to the Beckman TL100 tabletop centrifuge and is complete in 3 h. This fast, simple method produces high quality RNA, suitable for use in RNase protection assays, polymerase chain reaction analysis, and Northern analysis. This purification procedure may be applicable to other sources, from which RNA isolation is complicated by the presence of abundant cell wall or matrix components.
Assuntos
Cartilagem Articular/química , Centrifugação com Gradiente de Concentração , Proteoglicanas/análise , RNA/isolamento & purificação , Ácido Trifluoracético/química , Animais , Northern Blotting , Soluções Tampão , Bovinos , Guanidinas/química , RNA/análise , Tiocianatos/químicaRESUMO
Human peripheral blood monocytes and neutrophils secrete TGF-beta. Activation of monocytes with LPS stimulates the secretion of TGF-beta; however, the production of TGF-beta by neutrophils was not altered by treatment with LPS. The secreted TGF-beta appears to be in a fully active form since acid treatment of the conditioned medium does not increase the amount of TGF-beta activity. TGF-beta 1 transcripts were detected at similar levels in both activated and nonactivated monocytes and neutrophils, suggesting that the increase in TGF-beta secretion after activation of monocytes is regulated by a posttranscriptional mechanism. Western blot analysis with anti-N-terminal TGF-beta 1 peptide antibodies indicate the leukocyte-derived TGF-beta is beta 1. In addition, TGF-beta 1 transcripts were detected in rat peritoneal macrophages and in a differentiating human hematopoietic tumor cell line (HEL). The ability of inflammatory cells such as monocytes and neutrophils to produce TGF-beta may play an important role in the function of these cells in wound repair, in the immune response, and in the pathogenesis of fibrotic diseases.
Assuntos
Monócitos/metabolismo , Neutrófilos/metabolismo , Fatores de Crescimento Transformadores/metabolismo , Animais , Complexo Antígeno-Anticorpo/farmacologia , Linhagem Celular , Endotoxinas/farmacologia , Regulação da Expressão Gênica , Células-Tronco Hematopoéticas/citologia , Humanos , Immunoblotting , Ratos , Ratos Endogâmicos , Fatores de Crescimento Transformadores/análise , Fatores de Crescimento Transformadores/genéticaRESUMO
We have carried out a comparison of cartilage degeneration that develops spontaneously with age in the Wistar and Fischer 344 rats. Young adult (6-month-old) Wistar rats were essentially free of any relevant lesion in the articular cartilage of the knee. Aged (24-month-old) Wistar rats had minimal to mild lesions found mainly in the medial tibial plateau. These lesions were indicated by a focal loss of toluidine blue staining and limited fibrillation. Approximately 68% of the old Wistar rats had some degree of pathologic change. In comparison, 100% of the Fischer 344 rats examined, both young and old, had cartilage degeneration. However, the severity of the lesions increased with age. These two rat strains have variable degrees of spontaneous age-associated cartilage degeneration and would be suitable for testing treatments to prevent or repair the cartilage damage.
Assuntos
Envelhecimento/patologia , Doenças das Cartilagens/patologia , Cartilagem Articular/patologia , Modelos Animais de Doenças , Ratos Endogâmicos F344 , Ratos Wistar , Animais , Masculino , Proteoglicanas/análise , RatosRESUMO
We provide evidence for apoptosis in Alzheimer's disease using the in situ labeling technique TUNEL (terminal transferase-mediated dUTP-biotin nick end labeling). The technique specifically detects apoptotic cells by utilizing terminal transferase to incorporate biotinylated nucleotides into the fragmented DNA of apoptotic cells. The labeled cells are visualized by reaction with avidin peroxidase and a suitable substrate. Sections from the hippocampus of Alzheimer-diseased (AD) brains and non-AD brains were examined for apoptosis. While considerable variation in the quantity of apoptotic cells was observed among individual samples, the incidence of apoptosis in AD brains was elevated in comparison to age-matched, non-AD brains in specific regions of the hippocampal formation. Immunostaining indicated that both neurons and astrocytes were undergoing apoptosis, although the majority of the TUNEL-positive cells appeared to be glial, based on the location of the stained cells. These data suggest that apoptosis may be involved in both the primary neuronal cell loss and in the glial response that is a component of AD.
Assuntos
Doença de Alzheimer/patologia , Apoptose , Encéfalo/patologia , Idoso , Idoso de 80 Anos ou mais , Doença de Alzheimer/metabolismo , Encéfalo/metabolismo , Dano ao DNA , DNA Nucleotidiltransferases/fisiologia , Feminino , Técnicas Genéticas , Humanos , Técnicas Imunológicas , Masculino , Pessoa de Meia-Idade , Doença de Parkinson/patologia , Valores de Referência , Coloração e RotulagemRESUMO
It has previously been shown that fibroblastic cells transformed by SV40 exhibit a reduced requirement for PDGF for growth. In addition, NIH/3T3 cells lose both their chemotactic response to PDGF and specific cell surface binding of PDGF after transformation with SV40. We have now examined whether the SV40 transformed NIH/3T3 cells are producing a factor which acts similarly to PDGF. Our studies indicate that NIH/3T3 cells transformed with SV 40 produce a factor which shares many biological properties with PDGF. We were unable to detect this activity in conditioned media from nontransformed NIH/3T3 cells. The SV40/NIH/3T3 derived factor appears to possess both chemotactic and mitogenic activity for connective tissue cells but not endothelial or epithelial cells. Furthermore, in preliminary studies, this activity competes with 125I-PDGF for binding to smooth muscle cells. The biochemical properties of the SV40/NIH/3T3 derived factor are different from those of PDGF. The SV40 activity appears to reside in a heat labile acidic protein (pI less than 7.0) of MW less than 30,000 whereas PDGF is a heat stable basic protein (pI9.8) of 30,000 MW. Production of this factor may play a role in the decreased serum requirement for cell replication exhibited by SV40-transformed NIH/3T3 cells by supplying the cells with their own PDGF-like growth factor.
Assuntos
Transformação Celular Viral , Fatores Quimiotáticos/biossíntese , Mitógenos , Animais , Ligação Competitiva , Linhagem Celular , Fatores Quimiotáticos/análise , Fatores Quimiotáticos/metabolismo , Fatores Quimiotáticos/farmacologia , Quimiotaxia , Endotélio/citologia , Células Epiteliais , Fibroblastos/citologia , Temperatura Alta , Camundongos , Mitógenos/análise , Mitógenos/metabolismo , Mitógenos/farmacologia , Peso Molecular , Músculo Liso/citologia , Fator de Crescimento Derivado de Plaquetas/metabolismo , Receptores de Superfície Celular/metabolismo , Receptores do Fator de Crescimento Derivado de Plaquetas , Vírus 40 dos Símios/fisiologiaRESUMO
Migration of endothelial cells is involved in normal and pathological angiogenesis and in re-endothelialization after vascular injury or rupture of atherosclerotic plaques. Several types of endothelial cells are known to synthesize basic fibroblast growth factor (bFGF); in some of these, migration is increased by exogenous bFGF and inhibited by anti-bFGF antibodies. Using immunocytochemical techniques and RNase protection analysis, we studied endothelial cells from bovine coronary arteries and veins as well as from adrenal microvessels. We found that bFGF mRNA and peptide were present in confluent endothelial cells and were upregulated during migration stimulated by removal of some cells from the monolayer. During migration, extracellular matrix stores of bFGF were depleted, and bFGF immunoreactivity began to accumulate in the cytoplasm of endothelial cells between 2 and 6 hours. After migration had begun, but before the initiation of DNA synthesis, bFGF immunoreactivity increased in the nuclei and nucleoli. Exogenous bFGF stimulated endothelial migration, and antibodies to bFGF markedly inhibited migration, suggesting that an intracrine function of nuclear bFGF is not sufficient for cell migration. In all three types of endothelial cells studied, bFGF was identified as an endogenous regulator, but not as the sole regulator, or migration. Moreover, bFGF expression and subcellular localization were found to be regulated during endothelial cell migration.
Assuntos
Endotélio Vascular/metabolismo , Fator 2 de Crescimento de Fibroblastos/metabolismo , Frações Subcelulares/metabolismo , Animais , Autorradiografia , Bovinos , Movimento Celular , Endotélio Vascular/citologia , Endotélio Vascular/fisiologia , Fator 2 de Crescimento de Fibroblastos/genética , Imuno-Histoquímica , Hibridização de Ácido Nucleico , RNA Mensageiro/metabolismo , Ribonucleases , CicatrizaçãoRESUMO
To gain insight into the mechanisms of synthesis, storage, and release of basic fibroblast growth factor (bFGF), we studied the immunohistochemical localization of bFGF in bovine coronary artery, coronary sinus, and adrenal capillary endothelial cells grown in culture. Light and electron microscopic immunohistochemical studies were performed using the ABC immunoperoxidase method on p-formaldehyde-fixed cells. Five different anti-bFGF antibodies gave similar results in all cell types. In subconfluent cells, immunoreactivity was noted in the nuclear chromatin, nucleoli, cytosol, cytoplasmic vesicles (some of which appeared to fuse with the plasma membrane), and extracellular matrix. No reaction was found in endoplasmic reticulum or Golgi zones. Confluent cells demonstrated less immunoreactivity in the nuclei and cytosol but more in the extracellular matrix. Some cells of senescent morphology showed only cytoplasmic staining; however, no cells were found with only nuclear staining. Biochemical studies showed that three forms of bFGF (18, 24, and 26 kDa) were present in endothelial cells and varied with different culture conditions. Protection analysis indicated that bFGF mRNA is less abundant in postconfluent cells than in subconfluent cells. These data suggest that subconfluent cells synthesize bFGF and transport it into the nucleus and exocytotic vesicles, while confluent cells synthesize little bFGF but store it in extracellular matrix, cytoplasmic vesicles, and nuclei.