[Enterochromaffin cells of the intestinal mucosa during long-term parenteral and intragastric feeding]. / Sostoianie enterokhromaffinnykh kletok slizistoi obolochki kishechnika pri dlitel'nom parenteral'nom i vnutrizheludochnom pitanii.

The authors studied the response of enterochromaffin cells (Ec-cells) of the duodenal mucosa of rats during parenteral and intragastric administration of food mixtures containing a fatty emulsion venolipid (Japan) and of food mixtures containing no fat. The food mixture was administered round the clock, uniformly throughout 15 days. The Ec-cells were detected by the argentaffinity method modified by Phontane . It was discovered that during parenteral and intragastric administration of the food mixture containing a fatty emulsion, the amount of the Ec-cells in the duodenal mucosa was 3 times greater as compared with control. There were cells with varying intensity of the argentaffinity response, which attests to an active release of hormones synthesized by the Ec-cells. During intragastric administration of the food mixture containing no fatty emulsion, the status of the Ec-cells was the most similar to that seen in control. This is suggestive of an important role of the diet fat for changes in the Ec-cells. The changes found should be taken into consideration in the assessment of the effects of parenteral and intragastric feeding on the body and intestine and in the choice of the most optimal methods of feeding.

[Changes in the number of Ec cells in the small intestine and the serotonin level in the blood plasma of fasting rats]. / Izmeneniia kolichestva Ec-kletok v tonkoi kishke i urovnia serotonina v plazme krovi krys pri golodanii.

A study was made of the amount of Ec-cells in the small intestine mucosa and blood plasma serotonin of rats in health and fasting. It was established that 24 hours after food deprivation the amount of Ec-cells rises approximately 2-fold as compared with control. The cells demonstrate the intensification of the argentaffin reaction. The content of serotonin in blood plasma increases 2-fold accordingly. On day 3 of fasting the amount of Ec-cells and intensity of the argentaffin reaction decrease to normal, whereas the content of blood plasma serotonin does not change essentially. This may be linked with a massive release of serotonin to blood and depletion of Ec-cells because of which the threshold of their histochemical demonstration is reduced. On day 7 of food deprivation the amount of Ec-cells and the intensity of the argentaffin reaction increase again but the serotonin content dramatically falls down. This phenomenon may be related to the derangement of serotonin release to blood or to the transformation from the synthesis of serotonin to the synthesis of some other hormone, most likely melatonin.

[Changes in the glial condition in different sections of the rat brain after stopping the systemic circulation]. / Izmenenie sostoianiia glii v razlichnykh otdelakh mozga krys posle ostanovki sistemnogo krovoobrashcheniia.

The effect of ischemia on the expression of GFAP in astrocytes of cerebrum, hippocampus and cerebellum was studied on rat clinical death model. Cardiac arrest was induced by 10-or 15-min intrathoracic compression of the heart vascular bundle. Immunohistochemical staining showed that GFAP immunoreactivity significantly increased in the white matter, and GFAP-expressing astrocytes appeared in the gray matter. The reaction activity correlated with ischemia duration and phases of postresuscitation process. The obtained data are indicative of possible changes in the astrocytes condition in the absence of manifest lesions of neurons. This brings up the question of the role of glia homeostasis derangements in the formation of brain postresuscitation pathology.