Effects of regulatory B cells on intracranial aneurysms by mediating IL-1ß/IL-1R pathways.

The purpose of this study was to explore the effects of regulatory B-cells (Breg) on intracranial aneurysms by mediating IL-1ß/IL-1R pathways.  The study involved 60 patients undergoing angiography in a hospital from January to June 2022, divided into two groups: 30 with intracranial aneurysms (observation group) and 30 without (control group). Researchers extracted peripheral blood mononuclear cells (PBMC) to analyze the proportion of CD19+CD24hiCD38hiB cells using flow cytometry. These cells, along with T-cells and regulatory T-cells (Treg), were isolated through magnetic bead cell sorting. Following co-culture, the proliferation of T-cells and their related secretory factors were assessed. Additionally, Breg cells, treated with an IL-1R receptor blocker or IL-1R expression adenovirus, were studied to evaluate the levels of IL-10 and TGF-ß. In the study, the observation group showed lower levels of CD19+CD24hiCD38hiB cells, IL-10, and TGF-ß in PBMC than the control group (P<0.05). T-cell proportions were similar in both groups pre and post co-culture (P>0.05). Post co-culture, IFN-γ decreased while IL-4 increased in both groups. The observation group had higher IFN-γ and lower IL-4 than the control group (P<0.05). TNF-α in CD8+T cells, and granzyme B and perforin mRNA levels decreased post co-culture but were higher in the observation group (P<0.05). IL-10 and TGF-ß in Treg cells increased in both groups post co-culture but were lower in the observation group (P<0.05). The observation group also had fewer CD19+IL-1R+IL-10+B cells (P<0.05). After IL-1R blocker addition, IL-10 and TGF-ß in the supernatant decreased in the observation group (P<0.05). Following transfection, IL-1 and TGF-ß levels increased compared to the blank group (P<0.05). The function of peripheral blood CD19+CD24hiCD38hiB cells is impaired in patients with intracranial aneurysms, which may be related to IL-1ß/IL-1R pathways disorder.

Designing thermally activated delayed fluorescence emitters with through-space charge transfer: a theoretical study.

Recently, thermally activated delayed fluorescence (TADF) molecules with through-space charge transfer (TSCT) features have been widely applied in developing organic light-emitting diodes with high luminescence efficiencies. The performance of TSCT-TADF molecules depends highly on their molecular structures. Therefore, theoretical investigation plays a significant role in designing novel highly efficient TSCT-TADF molecules. Herein, we theoretically investigate two recently reported TSCT-TADF molecules, 1'-(2,12-di-t-butyl[1,4]benzoxaborinino[2,3,4-kl]phenoxaborinin-7-yl)-10-phenyl-10H-spiro[acridine-9,9'-fluorene] (AC-BO) and 1-(2,12-di-t-butyl[1,4]benzoxaborinino[2,3,4-kl]phenoxaborinin-7-yl)-9',9'-dimethyl-9'H-spiro [fluorene-9,5'-quinolino[3,2,1-de]acridine](QAC-BO). The calculated photophysical properties (e.g. excited state energy levels and luminescence properties) for these two compounds are in good agreement with experimental data. Based on the systematic analysis of structure-performance relationships, we design three novel TSCT-TADF molecules with high molecular rigidity and evident TSCT features, i.e., DQAC-DBO, DQAC-SBO, and DQAC-NBO. They exhibit deep-blue light emissions and fast reverse intersystem crossing rates (KRISCs). Our calculations demonstrate that the nearly coplanar orientation of the donor and acceptor is critical to achieve remarkable KRISCs and fluorescence efficiencies in TSCT-TADF molecules.

Enhancing Reverse Intersystem Crossing in Triptycene-TADF Emitters: Theoretical Insights into Reorganization Energy and Heavy Atom Effects.

Thermally activated delayed fluorescence (TADF) emitters based on the triptycene skeleton demonstrate exceptional performance, superior stability, and low efficiency roll-off. Understanding the interplay between the luminescent properties of triptycene-TADF molecules and their assembly environments, along with their excited-state characteristics, necessitates a comprehensive theoretical exploration. Herein, we predict the photophysical properties of triptycene-TADF molecules in a thin film environment using the quantum mechanics/molecular mechanics method and quantify their substantial dependency on the heavy atom effects and reorganization energies using the Marcus-Levich theory. Our calculated photophysical properties for two recently reported molecules closely align with experimental values. We design three novel triptycene-TADF molecules by incorporating chalcogen elements (O, S, and Se) to modify the acceptor units. These newly designed molecules exhibit reduced reorganization energies and enhanced reverse intersystem crossing (RISC) rates. The heavy atom effect amplifies spin-orbit coupling, thereby facilitating the RISC process, particularly at a remarkably high rate of ∼109 s-1.

The application of impantable sensors in the musculoskeletal system: a review.

As the population ages and the incidence of traumatic events rises, there is a growing trend toward the implantation of devices to replace damaged or degenerated tissues in the body. In orthopedic applications, some implants are equipped with sensors to measure internal data and monitor the status of the implant. In recent years, several multi-functional implants have been developed that the clinician can externally control using a smart device. Experts anticipate that these versatile implants could pave the way for the next-generation of technological advancements. This paper provides an introduction to implantable sensors and is structured into three parts. The first section categorizes existing implantable sensors based on their working principles and provides detailed illustrations with examples. The second section introduces the most common materials used in implantable sensors, divided into rigid and flexible materials according to their properties. The third section is the focal point of this article, with implantable orthopedic sensors being classified as joint, spine, or fracture, based on different practical scenarios. The aim of this review is to introduce various implantable orthopedic sensors, compare their different characteristics, and outline the future direction of their development and application.

Fabrication of three-dimension hierarchical structure CuO nanoflowers and their antifungal mechanism against Bipolaris sorokiniana.

Nanomaterials are widely investigated in sustainable agriculture owing to their unique physicochemical properties, especially Cu-based nanomaterial with eco-friendliness and essential for plant. However, the effect of CuO nanomaterial on Bipolaris sorokiniana (B. sorokiniana) is yet to be systematically understood. In this study, a three-dimension hierarchical structure CuO nanoflower (CuO NF) with ultrathin petals and excellent dispersibility in water was constructed and proved to have outstanding antifungal activity against B. sorokiniana with the inhibition rate of 86 % in mycelial growth, 74 % in mycelial dry weight and 75 % in conidial germination. Furthermore, the antifungal mechanism was assigned to the production of reactive oxygen species in intracellular caused by antioxidant mimicking activity of CuO NF to damage of cell membrane integrity and result cellular leakage. Additionally, the good control effect of CuO NF on wheat diseases caused by B. sorokiniana was demonstrated through pot experiment. This article firstly reveals the antifungal activity and mechanism of CuO NF on B. sorokiniana, and establishes the relationship between enzyme-like activity of CuO NF and its antifungal activity, which provides a promising application of Cu-based nanomaterial as nanofungicide in plant protection and a theoretical foundation for structure design of nanomaterials to improve their antifungal activities.

Glycine/alginate-based piezoelectric film consisting of a single, monolithic ß-glycine spherulite towards flexible and biodegradable force sensor.

Development of piezoelectric biomaterials with high piezoelectric performance, while possessing excellent flexibility, biocompatibility, and biodegradability still remains a great challenge. Herein, a flexible, biocompatible and biodegradable piezoelectric ß-glycine-alginate-glycerol (Gly-Alg-Glycerol) film with excellent in vitro and in vivo sensing performance was developed. Remarkably, a single, monolithic ß-glycine spherulite, instead of more commonly observed multiple spherulites, was formed in alginate matrix, thereby resulting in outstanding piezoelectric property, including high piezoelectric constant (7.2 pC/N) and high piezoelectric sensitivity (1.97 mV/kPa). The Gly-Alg-Glycerol film exhibited superior flexibility, enabling complex shape-shifting, e.g. origami pigeon, 40% tensile strain, and repeated bending and folding deformation without fracture. In vitro, the flexible Gly-Alg-Glycerol film sensor could detect subtle pulse signal, sound wave and recognize shear stress applied from different directions. In addition, we have demonstrated that the Gly-Alg-Glycerol film sensor sealed by polylactic acid and beeswax could serve as an in vivo sensor to monitor physiological pressure signals such as heartbeat, respiration and muscle movement. Finally, the Gly-Alg-Glycerol film possessed good biocompatibility, supporting the attachment and proliferation of rat mesenchymal stromal cells, and biodegradability, thereby showing great potential as biodegradable piezoelectric biomaterials for biomedical sensing applications.