RESUMO
The epidemiology of urinary tract infections (UTI) by Staphylococcus saprophyticus has not been fully characterised and strain typing methods have not been validated for this agent. To evaluate whether epidemiological relationships exist between clusters of pulsed field gel-electrophoresis (PFGE) genotypes of S. saprophyticus from community-acquired UTI, a cross-sectional surveillance study was conducted in the city of Rio de Janeiro, Brazil. In total, 32 (16%) female patients attending two walk-in clinics were culture-positive for S. saprophyticus. Five PFGE clusters were defined and evaluated against epidemiological data. The PFGE clusters were grouped in time, suggesting the existence of community point sources of S. saprophyticus. From these point sources, S. saprophyticus strains may spread among individuals.
Assuntos
Infecções Estafilocócicas/microbiologia , Staphylococcus saprophyticus/isolamento & purificação , Infecções Urinárias/microbiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Técnicas de Tipagem Bacteriana , Brasil/epidemiologia , Criança , Pré-Escolar , Análise por Conglomerados , Infecções Comunitárias Adquiridas/epidemiologia , Infecções Comunitárias Adquiridas/microbiologia , Estudos Transversais , Eletroforese em Gel de Campo Pulsado , Feminino , Humanos , Lactente , Pessoa de Meia-Idade , Vigilância da População , Gravidez , Infecções Estafilocócicas/epidemiologia , Staphylococcus saprophyticus/classificação , Infecções Urinárias/epidemiologia , Adulto JovemRESUMO
Staphylococcus saprophyticus is an uropathogen belonging to the human microbiota and is responsible for community-acquired infections of the urinary tract. Identification of Staphylococcus species by biochemical tests is laborious and costly when compared to routine laboratory tests. Because of their high sensitivity and specificity, molecular methods are better suited for accurate identification of Staphylococcusspp. Therefore, the goal of this work was to standardize a polymerase chain reaction (PCR) protocol using species-specific primers, based on the heat-shock repressor coding hrcA gene, for the identification of S.saprophyticus. A total of 142 S. saprophyticus strains were obtained from different sources, including clinical, environmental, and foodborne strains. We also included 98 strains of Staphylococcus spp. to further validate the proposed method. Reliable results for the detection of S. saprophyticus isolates were obtained for 100% of the strains evaluated. The results were in accordance with matrix-assisted laser desorption ionization-time of flight mass spectrometry identification, thus highlighting the applicability of species-specific PCR for the molecular identification of S. saprophyticus.
Assuntos
Técnicas de Diagnóstico Molecular/métodos , Reação em Cadeia da Polimerase/métodos , Proteínas Repressoras/genética , Staphylococcus saprophyticus/classificação , Staphylococcus saprophyticus/isolamento & purificação , Microbiologia Ambiental , Feminino , Microbiologia de Alimentos , Humanos , Gravidez , Complicações Infecciosas na Gravidez/microbiologia , Sensibilidade e Especificidade , Infecções Estafilocócicas/microbiologia , Staphylococcus saprophyticus/genética , Infecções Urinárias/microbiologiaRESUMO
The epidemiology of urinary tract infections (UTI) by Staphylococcus saprophyticus has not been fully characterised and strain typing methods have not been validated for this agent. To evaluate whether epidemiological relationships exist between clusters of pulsed field gel-electrophoresis (PFGE) genotypes of S. saprophyticus from community-acquired UTI, a cross-sectional surveillance study was conducted in the city of Rio de Janeiro, Brazil. In total, 32 (16%) female patients attending two walk-in clinics were culture-positive for S. saprophyticus. Five PFGE clusters were defined and evaluated against epidemiological data. The PFGE clusters were grouped in time, suggesting the existence of community point sources of S. saprophyticus. From these point sources, S. saprophyticus strains may spread among individuals.