RESUMO
AIMS: To determine whether myeloid proliferation was monoclonal or polyclonal in a woman with chronic neutrophilic leukaemia and myeloma. METHODS: The X-linked probe, M27 beta was used to determine the clonality of the neutrophil population by analysis of restriction fragment length polymorphisms and X inactivation pattern. RESULTS: A polyclonal pattern of X inactivation was obtained for the neutrophil population in this patient. CONCLUSION: The myeloid expansion in chronic neutrophilic leukaemia associated with myeloma represents a polyclonal reactive response to the plasma cell clone rather than a co-existent myeloproliferative disorder.
Assuntos
Sondas de DNA , Leucemia Neutrofílica Crônica/etiologia , Mieloma Múltiplo/complicações , Idoso , Southern Blotting , Feminino , Ligação Genética , Humanos , Leucemia Neutrofílica Crônica/genética , Mieloma Múltiplo/genética , Polimorfismo de Fragmento de Restrição , Cromossomo XRESUMO
Initial Rh phenotyping of a man with hemolytic anemia, his wife, and son appeared to exclude paternity. No exclusion was found in other blood groups or in the human leukocyte antigen (HLA) system; excluding Rh, the paternity index was 98.58 percent. Samples from these three family members, and two other family members, were tested with additional Rh antisera. The results indicated that the propositus has an Rhmod phenotype with expression of c, weak e, and very weak D, E, and G antigens. To support this hypothesis, DNA analysis of the RHD and RHCE genes was performed on the five family members. Polymerase chain reaction (PCR) products from exons 2 and 5 were analyzed by denaturing gradient gel electrophoresis (DGGE). The DNA results corroborated the serologic findings and refuted the exclusion of paternity.