RESUMO
We examined the protective effects of ultrafiltered XinMaiJia (XMJ) extract in a hydrogen peroxide (H2O2)-induced injury model in human umbilical vein endothelial cells (HUVECs) and determined the corresponding changes in the Na(+)-H(+) exchanger (NHE1) protein content and NHE1 gene expression. H2O2-induced HUVECs were treated with different concentrations of XMJ extract and the corresponding changes in morphology, activity, membrane permeability, biochemical indicators, cytokine concentration, NHE1 protein content, and NHE1 gene expression were determined. H2O2 significantly promoted HUVEC injury, whereas ultrafiltered XMJ extract significantly improved the morphological changes in injured HUVECs, increased their activity, and decreased NHE1 gene and protein expression, as well as limited the decrease in membrane permeability and expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, interleukin (IL)-1, IL-6, and nuclear factor-kB. Ultrafiltered XMJ extract inhibited H2O2-induced HUVEC injury by inhibiting NHE1 activity.
Assuntos
Proteínas de Transporte de Cátions/metabolismo , Medicamentos de Ervas Chinesas/farmacologia , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana/metabolismo , Peróxido de Hidrogênio/efeitos adversos , Substâncias Protetoras/farmacologia , Trocadores de Sódio-Hidrogênio/metabolismo , Proteínas de Transporte de Cátions/genética , Permeabilidade da Membrana Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Citocinas/biossíntese , Relação Dose-Resposta a Droga , Regulação para Baixo , Expressão Gênica , Células Endoteliais da Veia Umbilical Humana/patologia , Humanos , Trocador 1 de Sódio-Hidrogênio , Trocadores de Sódio-Hidrogênio/genética , Superóxido Dismutase/metabolismoRESUMO
Zinc finger protein 191, ZNF24 and Zfp191 in both humans and mice belong to the SCAN domain subfamily of Krüppel-like zinc finger transcription factors. Previous studies have suggested that Zfp191 is a pleiotropic factor involved in embryonic development, hematopoiesis and tumorigenesis. However, little is known about its target genes or its role in other physiological and pathological processes. We have identified the putative target genes of Zfp191, using an in silico genome-wide scan. Three hundred and fifty-five putative target genes were identified, which were enriched into the pathways of immune response according to the pathway analysis. These targets indicated that Zfp191 may function as a mediator of the immune response. This was verified in mice heterozygous for Zfp191 (Zfp191(+/-)) using a lipopolysaccharide (LPS)-induced endotoxic shock model. After LPS injection, Zfp191(+/-) mice produced significantly less IL-1ß and IL-6 compared to wild-type mice and were resistant to LPS-induced endotoxic shock. The loss of Zfp191 may suppress systemic inflammation by reducing these cytokine levels during LPS-induced endotoxic shock.
Assuntos
Proteínas de Transporte/genética , Resistência à Doença/genética , Resistência à Doença/imunologia , Técnicas de Inativação de Genes , Heterozigoto , Choque Séptico/genética , Choque Séptico/imunologia , Animais , Sequência de Bases , Sítios de Ligação , Biologia Computacional , Regulação da Expressão Gênica , Interleucina-1beta/sangue , Interleucina-6/sangue , Lipopolissacarídeos , Camundongos , Camundongos Knockout , Dados de Sequência Molecular , Especificidade de Órgãos , Ligação Proteica , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Choque Séptico/sangue , Choque Séptico/patologia , Análise de SobrevidaRESUMO
Hepatic wedge resection for colorectal liver metastasis has been reported to have a high incidence of positive surgical margins. Anatomic segmental resection is now widely practiced, although there are few data comparing segmental and wedge resection in terms of tumor clearance or long-term outcome. There were 267 patients who underwent liver resection for metastatic colorectal cancer between July 1985 and October 1998 at our institution who had either a wedge (n = 119) or segmental (n = 148) resection. Patient, tumor, and treatment data were compared, actuarial survival was determined, and prognostic factors were analyzed. Anatomic segmental resection was associated with similar blood loss, operative time, and complications as wedge resection. Segmental resection had a significantly lower rate of positive margins (2% vs. 16%) compared to wedge hepatectomy (P <0.001). On univariate analysis, segmentectomy resulted in longer survival with a median of 53 months vs. 38 months for wedge hepatectomy (P = 0.015). Preoperative carcinoembryonic antigen level, positive margin of resection, and the presence of extrahepatic disease independently predicted survival on multivariate analysis. Anatomic segmental resection is a safe procedure and is superior to wedge resection as an oncologic operation for colorectal liver metastasis because it results in better tumor clearance and improved survival.
Assuntos
Adenocarcinoma/secundário , Adenocarcinoma/cirurgia , Neoplasias Colorretais/patologia , Hepatectomia/métodos , Neoplasias Hepáticas/secundário , Neoplasias Hepáticas/cirurgia , Adenocarcinoma/mortalidade , Adulto , Idoso , Idoso de 80 Anos ou mais , Intervalo Livre de Doença , Feminino , Humanos , Neoplasias Hepáticas/mortalidade , Masculino , Pessoa de Meia-Idade , Cidade de Nova Iorque/epidemiologia , Análise de Sobrevida , Resultado do TratamentoRESUMO
96% ethyl alcohol was injected into a diagonal branch of the left anterior descending coronary artery of 10 out of 12 anesthetized dogs, and saline solution was injected in the remaining 2. After chest closure, the dogs were subjected to ambulatory monitoring (H) during days 1, 2, 3 and 7. The electrophysiologic study (EPS) and signal-averaged electrocardiography. (SA-ECG) were performed before the injection and again at day 7 after injection. H failed in one dog which died of ventricular fibrillation. In the other 9 dogs that received alcohol injection, H showed frequent ventricular premature and ventricular tachycardia (VT) after injection; six of the 9 dogs sustained VT, which was not inducible by EPS. VT was not found in 2 control dogs receiving saline solution injection. The SA-ECG showed no ventricular late potentials in dogs receiving alcohol injection. Post-ablation ventricular arrhythmia (including VT) occurred after intracoronary ethyl alcohol injection in all dogs. Arrhythmogenicity markedly declined during the first 3 days and almost completely disappeared on day 7 after ablation. There was no evidence in favor of reentry as the mechanism of these arrhythmias. Enhanced automaticity was considered as the mechanism for ventricular arrhythmia.
Assuntos
Etanol/efeitos adversos , Taquicardia Ventricular/fisiopatologia , Animais , Vasos Coronários , Cães , Eletrocardiografia , Eletrofisiologia , Injeções Intra-Arteriais , Taquicardia Ventricular/induzido quimicamenteRESUMO
Transcoronary chemical ablation of ventricular tachycardia was recently performed in one patient with refractory ventricular tachycardia in our hospital. The procedure was successful. The arrhythmia was cured and no recurrence occurred in a 24-month period. The clinical application and mechanism of transcoronary chemical ablation were discussed.
Assuntos
Etanol/administração & dosagem , Taquicardia/tratamento farmacológico , Adulto , Cateterismo Cardíaco , Vasos Coronários , Humanos , Injeções Intra-Arteriais , MasculinoRESUMO
Thirty-seven reproducible ventricular tachycardias (VTs) were induced in 19 dogs after the onset of myocardial infarction. The site of origin of VT was localized in 19 (59%) of 32 VTs by ice epicardial mapping. After 0.3-1.2 ml of 95% ethanol was injected into a small coronary artery supplying the arrhythmogenic area, VT was no longer inducible in 10 of 14 dogs. Intramyocardial ethanol (1-3 ml) was injected into the site of origin of VT in 9 dogs including 4 with VTs reinduced after intracoronary ethanol. Six of these VTs were not reinduced. Thus, the total efficacy rate was 84%. In 7 dogs, after injection of 0.4-1.2 ml (mean 0.5 ml) of 95% ethanol into a small normal coronary artery, the extent of the changes in ECG, CK-MB and pathology was found to be related to the size of myocardial damage and to the dose of ethanol. The smaller the dose of ethanol was given and the more distal the branch of coronary artery into which the ethanol was injected, the smaller the myocardial damage was. The data demonstrated that intracoronary or intramyocardial injection of ethanol may ablate the experimental VT induced by programmed heart stimulation in dogs after myocardial infarction, indicating that this approach may be useful and meaningful in some selected instances. However, it is necessary to limit the myocardial damage as far as possible.
Assuntos
Etanol/administração & dosagem , Taquicardia/tratamento farmacológico , Animais , Vasos Coronários , Cães , Eletrocardiografia , Etanol/farmacologia , Hemodinâmica/efeitos dos fármacos , Hipotermia Induzida , Injeções Intra-Arteriais , Infarto do Miocárdio/complicações , Taquicardia/fisiopatologiaRESUMO
Space motion sickness (MS) is one of the most important problems in the field of space medicine. In order to prevent space MS, a new medicine, PMPA, has been prepared by means of synthesizing in our laboratory. The purposes of this study were to set up animal models of PMPA against MS, and to observe its effects on anti-MS, and to prove its function of antagonism to choline. Eight cats, forty rabbits and two hundred and ten rats were selected as animal subjects. The parallel swing stimulus, a method causing the reversal syndromes and tests of anti-choline function were used in our experiments. The results are as follows: (1) The score of MS symptoms in cats with PMPA or scopolamine (SCOP) is significantly lower than that in cats with placebo (p<0.01), while the incidences of efficiency and prevention of PMPA (87.5%, 75%) are higher than those of SCOP (75.0%, 50%) in cats. (2) PMPA of 1.6 mg/kg or 0.8 mg/kg could antagonize the reversal syndromes and repress reversal rotation significantly in rabbits like SCOP in comparison with placebo (p<0.01). (3) PMPA could inhibit tremor evoked by oxotremorine or by nicotine-procaine in rats like SCOP, and play an important role in the antagonism to central M-choline and N-choline receptors. The animal experiments demonstrate that PMPA is an effective medicine against MS with antagonism function to choline.
Assuntos
Antieméticos/uso terapêutico , Antagonistas Colinérgicos/uso terapêutico , Modelos Animais , Enjoo devido ao Movimento em Voo Espacial/tratamento farmacológico , Enjoo devido ao Movimento em Voo Espacial/prevenção & controle , Aceleração , Animais , Gatos , Ensaios Clínicos como Assunto , Relação Dose-Resposta a Droga , Medicamentos de Ervas Chinesas/uso terapêutico , Masculino , Oxotremorina/antagonistas & inibidores , Coelhos , Ratos , Escopolamina/uso terapêuticoRESUMO
The purpose of this study was to identify and quantify the availability of orthodontic literature for evidence-based clinical decision-making (ie, sound clinical studies of etiology, diagnosis, treatment, or prognosis meeting basic methodologic criteria for direct clinical use). This is a first step toward developing online decision analysis systems. A search strategy based on Medical Subject Headings (MeSH) for orthodontics was developed to examine MEDLINE using the Ovid Web Gateway search engine. Sensitive and specific methodologic search filters were then employed to identify the 4 categories of information. The results were then subdivided by year to identify trends and sorted to identify source of publications. In the period 1990 to 1998, the MEDLINE searches identified 6938 English-language articles about orthodontics. The mean number of articles (+/-SD) per year ranged from 42+/-25 for specific searches to 314+/-214 for sensitive searches. The number of articles identified by the specific or sensitive searches increased 14% to 21% annually. When subdivided by clinical category, the mean numbers of articles per year for specific and sensitive searches were respectively: etiology 19+/-15 and 91+/-37, diagnosis 11+/-5 and 80+/-35, therapy 3+/-1 and 50+/-23, and prognosis 10+/-7 and 93+/-33. Five dental journals accounted for nearly half of these publications. These results provide several key findings: (1) there is a substantial literature of clinically relevant information in orthodontics upon which to base clinical decisions; (2) the information appears to be balanced between etiology, diagnosis, treatment, and prognosis; (3) approximately 45% of the articles reside in 5 journals, whereas the remainder reside in approximately 66 other journals, making it difficult to stay current; (4) the number of articles is increasing significantly each year; (5) to stay current, one would need to read between 1 and 6 articles per week, 52 weeks per year; (6) these trends suggest the need for computer-based clinical knowledge systems; and (7) the methods used here can be immediately employed to identify the best and most current clinical orthodontic evidence.
Assuntos
Medicina Baseada em Evidências , Armazenamento e Recuperação da Informação , MEDLINE , Má Oclusão , Ortodontia Corretiva , Análise de Variância , Benchmarking/métodos , Bibliometria , Técnicas de Apoio para a Decisão , Humanos , Armazenamento e Recuperação da Informação/métodos , Armazenamento e Recuperação da Informação/estatística & dados numéricos , Modelos Lineares , Má Oclusão/diagnóstico , Má Oclusão/etiologia , Má Oclusão/terapia , Ortodontia Corretiva/normas , Ortodontia Corretiva/estatística & dados numéricos , Estatísticas não ParamétricasRESUMO
The presence of concealed accessory A-V pathways (CAP) was demonstrated in 14 out of 18 (78%) patients with supraventricular tachycardia (SVT) and narrow QRS complexes. Such a high percentage of CAP mediated AVRT is a quite unique finding. The importance of a standard programmed electrical stimulation protocol in documenting CAP is emphasized. Careful atrial mapping and premature ventricular stimulation during SVT are very helpful for diagnosis and localization of CAP. We considered that a V-A interval of 75 ms or more on intracardiac electrogram or a R-P interval of 85 ms or more on esophageal electrogram is a good criterion for AVRT utilizing an accessory pathway.
Assuntos
Sistema de Condução Cardíaco/fisiopatologia , Taquicardia Supraventricular/fisiopatologia , Adolescente , Adulto , Estimulação Elétrica , Eletrocardiografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Taquicardia Supraventricular/diagnósticoRESUMO
Six patients suspected to have "pacemaker syndrome" were studied with hemodynamic and electrophysiologic methods. Symptoms appeared after implantation of ventricular pacemaker (VVI). Mile symptoms such as dizziness and fatigue and severe symptom of heart failure were noted. The results of the study suggested that cardiac output decreases due to loss of atrial contribution to ventricular filling and also as a result of increase of systemic venous pressure. If the patient has V-A conduction, cardiac output will decrease further. Restoration of atrioventricular synchronization is essential in the treatment of the pacemaker syndrome.
Assuntos
Sistema de Condução Cardíaco/fisiopatologia , Marca-Passo Artificial/efeitos adversos , Síndrome do Nó Sinusal/terapia , Idoso , Baixo Débito Cardíaco/etiologia , Feminino , Insuficiência Cardíaca/etiologia , Hemodinâmica , Humanos , Masculino , Pessoa de Meia-Idade , SíndromeRESUMO
The electrophysiological effects of intravenous propafenone were studied with programmed electrical stimulation in 31 patients. Among them 29 cases with SVT (including WPW syndrome in 11, paroxysmal AF or Af in 3) and the other two cases were studied for other reasons. The results showed that: (1) Propafenone has significant effects on each part of the special conduction tissues of the heart as well as the accessory pathway. (2) It is an effective drug for treating AVNRT and the tachycardias associated with WPW syndrome. (3) There were no effects on sinus node function in all except one patient whose SNRT was prolonged to 2020 ms. (4) Neither blood pressure dropping nor other side effects were found during the studies.
Assuntos
Propafenona/uso terapêutico , Taquicardia Supraventricular/tratamento farmacológico , Adolescente , Adulto , Eletrocardiografia , Eletrofisiologia , Feminino , Humanos , Injeções Intravenosas , Masculino , Pessoa de Meia-Idade , Propafenona/administração & dosagem , Taquicardia Supraventricular/fisiopatologia , Síndrome de Wolff-Parkinson-White/tratamento farmacológico , Síndrome de Wolff-Parkinson-White/fisiopatologiaRESUMO
Objective. To investigate the patterns of electrogastrogram [correction of electrogastrogragm] (EGG) before, during and after vestibular stimulation. Method. 25 subjects were stimulated by Coriolis acceleration and 28 by Coriolis [correction of corolis] stimulation. Result. Dysrythmia of EGG and increase of tachygastria were recorded in all subjects during the stimulation. The period dominant frequency (PDF) of EGG shifted from 2.40-3.70 cpm to 3.70-10.00 cpm during Coriolis stimulus in subjects with nausea. Conclusion. It suggests that PDF could be a parameter in reflecting the level of motion sickness.