RESUMO
OBJECTIVE: To test and estimate the forensic application of Goldeneye™ DNA ID 25A Kit. METHODS: The kit was validated by a series of tests for accuracy, sensitivity, consistency, peak height balance, stability, and mixed samples through measured blood samples and other samples in routine casework. RESULTS: The peak height balance of the different loci was ≥ 42%. The genotyping results of the positive control DNA was accurate. The complete STR genotyping result could be obtained from 0.125 ng positive control DNA. CONCLUSION: Goldeneye™ DNA ID 25A Kit is suitable for criminal cases and DNA database in forensic practice.
Assuntos
Povo Asiático/genética , Impressões Digitais de DNA/normas , DNA/genética , Genética Forense/métodos , China , Cromossomos Humanos Y , Bases de Dados de Ácidos Nucleicos , Feminino , Genótipo , Humanos , Reação em Cadeia da Polimerase , Polimorfismo Genético , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
OBJECTIVE: To establish an effective method for testing DNA from the sperm smear. METHODS: We did exploratory research for ninety-one cases of the sperm smear which were accumulated by our laboratory during working hours. Chelex extraction method was used to extract DNA. The digested solution of sperm was purified and concentrated by using Silicon bead. STR loci were typed after PCR amplification by Profile Plus kit. RESULTS: Although there were a few sperm cells on the slide, it is easy to obtain good DNA typing result from it because the amalgamative method we used was high effective for DNA extraction. CONCLUSION: The DNA analysis of sperm smear could offer satisfactory typing results which would be useful in case.