RESUMO
This report describes a dog with recurrent atrial fibrillation and atrial flutter associated with chronic gastritis. Although no underlying structural heart disease was apparent antemortem or on gross post-mortem examination, chronic atrial myocarditis was seen on histopathological examination. Atrial myocarditis is a recognised cause of atrial fibrillation in human beings with presumed lone fibrillation, and an association between supraventricular tachyarrhythmias and infectious agents has been made.
Assuntos
Arritmias Cardíacas/veterinária , Doenças do Cão/diagnóstico , Gastrite/veterinária , Infecções por Helicobacter/veterinária , Miocardite/veterinária , Animais , Antibacterianos/uso terapêutico , Arritmias Cardíacas/diagnóstico , Arritmias Cardíacas/etiologia , Arritmias Cardíacas/terapia , Diagnóstico Diferencial , Doenças do Cão/terapia , Cães , Cardioversão Elétrica/métodos , Cardioversão Elétrica/veterinária , Eletrocardiografia/veterinária , Evolução Fatal , Gastrite/complicações , Gastrite/diagnóstico , Gastrite/terapia , Infecções por Helicobacter/complicações , Infecções por Helicobacter/diagnóstico , Infecções por Helicobacter/terapia , Masculino , Miocardite/complicações , Miocardite/diagnóstico , Miocardite/terapia , RecidivaRESUMO
The effect of salivary gland extract of the stable fly, Stomoxys calcitrans (L), on bovine lymphocyte proliferation was determined, and antibody reactivity to salivary gland proteins was characterized in cattle exposed to stable flies. Salivary glands were dissected from male and female flies (4-8 d after eclosion), and protein extracts were made by freeze-thaw cycles. Salivary gland extract (SGE, 1 and 5 microg) significantly inhibited mitogen-driven proliferation of bovine lymphocytes, compared with 1 and 5 microg of identically prepared midgut extract (ANOVA, P < 0.05). Phytohemagglutinin A (PHA) stimulated lymphocyte responses were suppressed by 61.7 and 79.5% (mean values) with 1 and 5 microg of SCE, whereas concanvalin A (Con A) stimulated responses were suppressed by 62.9 and 77.1% (1 and 5 microg). In contrast, midgut extract (1 and 5 microg) minimally suppressed PHA (12.7% +/- 12.6 and 18.7% +/- 15.5) and Con A-driven responses (13.8% +/- 20.5 and 24.6% +/- 14.9), respectively. Viability studies using propidium iodide and flow cytometry demonstrated that SGE was not cytotoxic. Two-color immunofluorescence studies identified T and B lymphocytes as the nonviable cells in the cultures. Western blot analysis of serum collected from five dairy cows during periods of low and high fly exposure identified an immunodominant 27 kDa protein among the salivary gland proteins. These results indicate that exposure of cattle to stable fly saliva during blood feeding results in an antibody response to salivary proteins and that the saliva has a potential to modulate T lymphocyte function.