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Bull Exp Biol Med ; 150(4): 515-8, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22268055

RESUMO

Ischemic stroke was modeled in the sensorimotor zone of the brain cortex in adult rats. Rat embryonic nervous tissue, neural stem cells from human olfactory epithelium, and rat fibroblasts (cell control) were implanted into the peri-infarction area of rats of different groups immediately after stroke modeling. Expression of BDNF mRNA was analyzed 7 days after surgery by real-time PCR. BDNF expression in cell preparation before their implantation was minimum. The expression of BDNF mRNA increased by 5-6 times in the areas of implantation of rat fibroblasts and human olfactory epithelium and by 23 times in the area of implantation of rat embryonic nervous tissue compared to periinfarction areas without cell implantation. These findings confirm the possibility of realization of the therapeutic effects of neural stem cells via expression of trophic factors.


Assuntos
Fator Neurotrófico Derivado do Encéfalo/metabolismo , Células-Tronco Neurais/metabolismo , Células-Tronco Neurais/transplante , Transplante de Células-Tronco , Acidente Vascular Cerebral/patologia , Animais , Encéfalo/metabolismo , Isquemia Encefálica/metabolismo , Fator Neurotrófico Derivado do Encéfalo/genética , Córtex Cerebral/patologia , Células-Tronco Embrionárias/transplante , Fibroblastos/metabolismo , Humanos , Masculino , Mucosa Olfatória/metabolismo , RNA Mensageiro/metabolismo , Ratos , Transplante Heterólogo
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