RESUMO
The effect of ethanol on the bioamin containing structures of the jejunum and ileum walls at different periods of chronic alcoholic intoxication was investigated. The quantitative description of the catecholamine and serotonin content in the enterocytes of epithelial lining villi, submucosa mast cells, crypt enterocytes and muscle membrane is given for the first time by means of luminescent microscopy and cyto spectrofluorimetry. It was established that in the initial period of chronic alcohol intoxication for 60 days in rats increased serotonin effect on intestinal functional structures was observed. On longer terms there is a disturbance of relationships of biogenic amines and the regulating role of catecholamines starts to prevail.
Assuntos
Intoxicação Alcoólica/metabolismo , Intestino Delgado/metabolismo , Neurotransmissores/metabolismo , Intoxicação Alcoólica/patologia , Animais , Catecolaminas/metabolismo , Modelos Animais de Doenças , Enterócitos/metabolismo , Enterócitos/patologia , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Intestino Delgado/patologia , Mastócitos/metabolismo , Mastócitos/patologia , Microscopia de Fluorescência , Ratos , Serotonina/metabolismo , Fatores de TempoRESUMO
Using the methods of luminescent microscopy and cytospectrofluorometry, biogenic amine-containing structures were studied in colon of rats subjected to alcohol intoxication of 2, 4 and 6 months' duration. It was demonstrated that 2- and 4-month-long alcohol intoxication of animals resulted in biogenic amine content increase in the mucosal surface epithelium, submucosal mast cells and adrenergic nerve fibers within the connective tissue bands in the muscular tunic. At the same time, 6 month-long intoxication was accompanied by a stabilization of bioamine content at the elevated level.