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Anal Bioanal Chem ; 410(8): 2127-2139, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29411089

RESUMO

The NISTmAb Reference Material (RM) 8671 is intended to be an industry standard monoclonal antibody for pre-competitive harmonization of best practices and designing next generation characterization technologies for identity, quality, and stability testing. It must therefore embody the quality and characteristics of a typical biopharmaceutical product and be available long-term in a stable format with consistent product quality attributes. A stratified sampling and analysis plan using a series of qualified analytical and biophysical methods is described that assures RM 8671 meets these criteria. Results for the first three lots of RM 8671 highlight the consistency of material attributes with respect to size, charge, and identity. RM 8671 was verified to be homogeneous both within and between vialing lots, demonstrating the robustness of the lifecycle management plan. It was analyzed in concert with the in-house primary sample 8670 (PS 8670) to provide a historical link to this seminal material. RM 8671 was verified to be fit for its intended purpose as a technology innovation tool, external system suitability control, and cross-industry harmonization platform. Graphical abstract The NISTmAb Reference Material (RM) 8671 is intended to be an industry standard monoclonal antibody for pre-competitive harmonization of best practices and designing next generation characterization technologies for identity, quality, and stability testing.


Assuntos
Anticorpos Monoclonais/química , Imunoglobulina G/química , Animais , Medicamentos Biossimilares/química , Cromatografia em Gel/métodos , Cromatografia em Gel/normas , Estabilidade de Medicamentos , Difusão Dinâmica da Luz/métodos , Difusão Dinâmica da Luz/normas , Eletroforese Capilar/métodos , Eletroforese Capilar/normas , Humanos , Microscopia/métodos , Microscopia/normas , Modelos Moleculares , Mapeamento de Peptídeos/métodos , Mapeamento de Peptídeos/normas , Estabilidade Proteica , Controle de Qualidade , Padrões de Referência , Espectrofotometria Ultravioleta/métodos , Espectrofotometria Ultravioleta/normas , Espectrometria de Massas em Tandem/métodos , Espectrometria de Massas em Tandem/normas
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