[Endogenous intoxication and biochemical protection in children with celiac disease: state assesment and correlation-regression analysis].

AIM: The purpose of the study was to determine the characteristics of endogenous intoxication parameters, biochemical protection and reveal their interaction in children with celiac disease. MATERIALS AND METHODS: 81 children aged from 1 to 16 years with celiac disease were examined in acute and remission periods. In erythrocytes, blood serum and urine we determined low and moderate molecular weight substances (LMMWS), oligopeptides OP); in erythrocytes--the value of erythrocyte mechanical hemolysis (MH), malondialdehyde (MDA) content, the activity of glutathione reductase GR) and superoxide dismutase (SOD); in blood serum--ceruloplasmin (CP) level, alcohol dehydrogenase (ADH) activity; in erythrocytes and blood serum--glutathione transferase (GT), and calculated intoxication index (II). RESULTS: In children with celiac disease in acute and remission periods LMMWS, OP, II levels in blood were statistically significantly high, while LMMWS level in urine was low. In both periods MH activity was high (p < 0.001), and GSR (p < 0.001) and SOD (p < 0.01) levels were low. We revealed the correlation between MDA and II (r = 0.67; p = 0.006), erythrocyte LMMWS and SOD (r = -0.61; p = 0.015), erythrocyte LMMWS and ADH (r = 0.62; p = 0.006), between GT and OP in urine (r = -0.31; p = 0.026), GTand MDA (r = 0.68; p = 0.000), GT and MH (r = -0.46; p = 0.004), between MDA and CP (r = 0.57; p = 0.002) that made it possible to develop the models of dependence of the parameters in relation to each other. CONCLUSION: In celiac disease there is endogenous intoxication. The changes of the first and the second phases of biotransformation, antioxidant protection is an essential factor of the disease pathogenesis, since they have an effect on endogenous intoxication formation that should be taken into consideration in therapy.

[The organization of comprehensive rehabilitation of the children presenting with inflammatory intestinal diseases and celiacia under the conditions of a health resort].

In this study we report the experience with the provision of rehabilitative services for the children presenting with long-standing chronic diseases of the digestive organs (ulcerative colitis, Crohn's disease, celiac disease) based at a regional health resort. The possibility of comprehensive rehabilitation was substantiated with the contribution by non-medical specialists including a psychologist, social worker, and educator. A number of organizational problems related to rehabilitation logistics and scientific support have been successfully solved. Standard programs comprising the mechanisms of comprehensive rehabilitation and methods for the evaluation of its effectiveness are proposed.

[A new procedure for evaluating the severity of childhood celiac disease].

The paper describes a new procedure for evaluating the severity of childhood celiac disease, the basis of which is to determine the salivary activity of the enzyme ornithine decarboxylase (ODC). The salivary ODC activity index of 0.0001 to 0.0080 ncat/ml suggests severe celiac disease; that of 0.0081-0.0145 and 0.0146-0.0410 ncat/ml indicates its moderate and mild forms, respectively. The development of this procedure is urgent since the typical forms of celiac disease, which make the determination of the degree of the disease difficult in most cases.

[Features of B group vitamin metabolism and criteria for providing them to children suffering from celiac disease]. / Osoobennosti obmena vitaminov gruppy B i kriterii obespechennosti imi detei, stradaiushchikh tseliakiei.

The investigation into the relationship between the concentration of vitamins B, the blood activity of vitamin B-dependent enzymes and the urinary excretion of their metabolites in 14 children of both sexes whose age was 4.5-10 years and who suffered from celiac disease has characterized their metabolic features. The criteria for vitamin B1 provision of these children differ from those in healthy children of the same age: the normal value of TDP effects is achieved with higher thiamine excretion which is more than 12 micrograms/h. Apparently, the plasma concentration of riboflavin greater than 14 ng/ml and its urinary excretion 6 micrograms/h should be taken as a criterion for normal vitamin B2 provision. Though due to a small proportion of the examined children, criteria for vitamin B6 and nicotinic acid could not be defined. The excretion of 4-pyridoxylic acid in the whole concentration ranges of pyridoxylic coenzymes in the plasma of sick children was essentially lower than that in healthy persons.

[The efficacy of the use of lactic acid lactobacterin enriched with zinc in the treatment of patients with celiac disease]. / Effektivnost' primeneniia kislomolochnogo laktobakterina, obogashchennogo tsinkom, v terapii bol'nykh tseliakiei.

Zinc sulfate-enriched lactic acid lactobacterin was used in the combined treatment of 23 children with celiac disease, aged from 1 to 10 years. A group of 23 children with celiac disease who received lactic acid lactobacterin without zinc were used as control. The patients treated with lactobacterin containing zinc showed a higher increase in body mass, total protein and zinc levels in the blood serum and elevated activity of metalloenzymes-ceruloplasmin and cytochrome oxidase.

[The criteria for the vitamin B2 and B6 allowances of children suffering from celiac disease]. / Kriterii obespechennosti vitaminami B2 i B6 detei, stradaiushchikh tseliakiei.

The relationship between the blood concentrations of vitamins B2 and B6 and excretion of their metabolites with the urine were studied in 34 children of both sexes aged 7 to 10 suffering from celiac disease. The study revealed that the criteria of adequate supply of these vitamins in the patients and healthy age-matched children differ. Riboflavin concentration of at least 90 ng/ml in red cells, at least 15 ng/ml in the blood plasma, and urinary excretion of at least 7 micrograms/h should be accepted as the criteria of this vitamin sufficiency in the organism. For vitamin B6 these values are 13 ng/ml pyridoxal phosphate in the blood plasma and urinary excretion of at least 47 micrograms/h of 4-pyridoxic acid.