RESUMO
Nasal polyps (NPs) are benign lesions of nasal and paranasal sinuses mucosa affecting 1-4 % of all adults. Nasal polyposis affects the quality of patient's life as it causes nasal obstruction, postnasal drainage, purulent nasal discharge, hyposmia or anosmia, chronic sinusitis, facial pain and snoring. Without treatment, the disease can alter the craniofacial skeleton in cases of extended growth of polyps. The development of NPs is caused by the hyperplasia of nasal or paranasal sinuses mucosa, and edema of extracellular matrix. This is usually the result of high concentration of high molecular mass hyaluronan (HA) which is either overproduced or accumulated from blood supply. The size of HA presents high diversity and, especially in pathologic conditions, chains of low molecular mass can be observed. In NPs, chains of about 200 kDa have been identified and considered to be responsible for the inflammation. The purpose of the present study was the investigation, in NPs and normal nasal mucosa (NM), of the expression of the wild-type and alternatively spliced forms of hyaluronidases, their immunolocalization, and the expression of HA synthases to examine the isoform(s) responsible for the increased amounts of HA in NPs. Hyaluronidases' presence was examined on mRNA (RT-PCR analysis) and protein (immunohistochemistry) levels. Hyaluronan synthases' presence was examined on mRNA levels. Hyaluronidases were localized in the cytoplasm of epithelial and inflammatory cells, as well as in the matrix. On mRNA level, it was found that hyal-1-wt was decreased in NPs compared to NM and hyal-1-v3, -v4 and -v5 were substantially increased. Moreover, HAS2 and HAS3 were the only hyaluronan synthases detected, the expression of which was almost similar in NPs and NM. Overall, the results of the present study support that hyaluronidases are the main enzymes responsible for the decreased size of hyaluronan observed in NPs; thus they behave as inflammatory agents. Therefore, they could be a potential target for the design of a more advanced treatment for nasal polyposis.
Assuntos
Regulação da Expressão Gênica , Glucuronosiltransferase/genética , Hialuronoglucosaminidase/genética , Pólipos Nasais/genética , RNA Mensageiro/genética , Adulto , Doença Crônica , Feminino , Glucuronosiltransferase/biossíntese , Humanos , Hialuronan Sintases , Hialuronoglucosaminidase/biossíntese , Imuno-Histoquímica , Masculino , Mucosa Nasal/metabolismo , Mucosa Nasal/patologia , Pólipos Nasais/enzimologia , Pólipos Nasais/patologia , Reação em Cadeia da Polimerase em Tempo RealRESUMO
BACKGROUND: Nasal polyps are benign lesions originating from the nasal mucosa or paranasal sinuses. The most important etiological factor seems to be increased hydration of epithelium and hyperplasia of the extracellular matrix, which may involve hyaluronan, a high molecular mass extracellular glycosaminoglycan. Degradation of hyaluronan proceeds through the action of specific hyaluronidases. OBJECTIVE: The aim of the present study was to investigate the hydrodynamic size of hyaluronan and the presence of the various hyaluronidase isoforms in nasal polyps. METHODS: Samples of polypoid mucosal tissue and normal nasal mucosa were obtained from twenty patients suffering from nasal polyposis. Zymographic analysis and western blotting were used to detect hyaluronidase activity. RESULTS: The results indicated the presence of hyaluronan of small molecular mass in all samples examined. About one third of it has a mean molecular mass of 240 kDa, exactly that required for the expression of inflammatory response. Laboratory analysis suggested that degradation of hyaluronan occurred through the action of three hyaluronidase isoforms: Hyal-1, Hyal-2 and PH-20. CONCLUSIONS: Since hyaluronan fragments of 200-250 kDa induce the expression of inflammatory cytokines, a specific role of hyaluronidases in the development or progression of nasal polyps may be concluded. Therefore, new treatment protocols may be proposed.
Assuntos
Hialuronoglucosaminidase/análise , Pólipos Nasais/enzimologia , Western Blotting , Humanos , Concentração de Íons de Hidrogênio , Isoenzimas/análiseRESUMO
BACKGROUND: The major proteoglycan of normal human larynx is aggrecan. In laryngeal carcinoma, aggrecan is depleted, with versican and decorin appearing in higher amounts. MATERIALS AND METHODS: Proteoglycans in laryngeal carcinoma samples were characterized immunohistochemically and using Western blotting; their expression was examined by RT-PCR. RESULTS: Aggrecan was totally removed in advanced cancer and its RT-PCR product was not identified. Both versican and decorin were overexpressed in cancer, versican much more than decorin. Decorin expression was higher than that of versican in the normal larynx; therefore, their disproportionate overexpression during cancer resulted in about equimolar expression. Both proteoglycans' expression correlated with their stage-related accumulation within the tissue. CONCLUSION: These data add to our previous findings and support the view that the levels of expression and the extent of accumulation and localization in the tumor stroma of structurally modified versican and decorin could be associated with the degree of aggressiveness of laryngeal carcinoma.
Assuntos
Carcinoma de Células Escamosas/metabolismo , Proteínas da Matriz Extracelular/biossíntese , Neoplasias Laríngeas/metabolismo , Proteoglicanas/biossíntese , Versicanas/biossíntese , Adulto , Idoso , Idoso de 80 Anos ou mais , Agrecanas/biossíntese , Agrecanas/genética , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Decorina , Proteínas da Matriz Extracelular/genética , Humanos , Imuno-Histoquímica , Neoplasias Laríngeas/genética , Neoplasias Laríngeas/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Proteoglicanas/genética , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Versicanas/genéticaRESUMO
Pseudoexfoliation syndrome (PEX) is an age-related condition, which may cause open-angle glaucoma and has increasing interest since it seems to affect additional human tissues, i.e., cardiovascular tissue, skin, and still lacks elucidated pathogenesis. Collagen type IX and HNK-1 epitope have been considered characteristic constituents of the aqueous humour of PEX patients, since their amounts were increased in PEX aqueous humour compared to normal eyes. Since it has been proposed that the initial manifestations of PEX syndrome occur in conjunctiva, the present study was undertaken to investigate the presence of the same antigens in tears of PEX patients and their possible use as the biochemical markers for early diagnosis. Tears of PEX patients and healthy individuals were subjected to western blotting analysis for various basement membrane components identified in aqueous humour. It was found that collagen type IX and HNK-1 epitope were present in tears, the amount of the former being increased 2.7 times compared to normal (P<0.05), surprisingly high as compared with total protein or lysozyme activity in tears, which were found to be increased in PEX patients about 25% with no statistical differences (P approximately 0.4). The results suggest the possible use of tears' collagen type IX for the diagnosis of PEX syndrome.
Assuntos
Antígenos CD57/metabolismo , Colágeno Tipo IX/metabolismo , Epitopos/metabolismo , Síndrome de Exfoliação/metabolismo , Lágrimas/química , Idoso , Idoso de 80 Anos ou mais , Eletroforese em Gel Bidimensional , Matriz Extracelular/química , Proteínas do Olho/metabolismo , Humanos , Pessoa de Meia-Idade , Muramidase/metabolismo , Lágrimas/enzimologiaRESUMO
Squamous cell laryngeal carcinoma undergoes significant structural-related modifications of the extracellular matrix components (ECM), the most characteristics being the presence of degraded collagen, aggrecan and hyaluronan. We examined the presence of hyaluronidase and of the cellular hyaluronan receptor CD44 during the various stages of cancer. ECM components were extracted by using PBS, 4 M GdnHCl and 4 M GdnHCl-0.1% Triton-X 100 sequentially and hyaluronidase and CD44 analyzed by zymography and immunochemistry techniques. Total RNA was also extracted and the mRNA of the various hyaluronidases and of CD44 was analyzed after amplification with RT-PCR. Hyaluronidase was detected as a double band of 45 and 55 kDa molecular mass, only in cancer samples. The analysis of mRNA indicated an aberrant expression of PH-20, the testicular-type hyaluronidase, at late stages of cancer and an overexpression of HYAL1 only at stage IV. In addition, CD44 was identified in two protein bands of 80 and 64 kDa in cancer samples. The analysis of mRNA showed that hyaluronan receptor was expressed in a stage-related order. Thus, it could be suggested that in laryngeal squamous cell carcinoma, cancer cells migrated and proliferated under the influence of small molecular mass hyaluronan, by expressing increased amounts of its receptor.
Assuntos
Carcinoma de Células Escamosas/metabolismo , Regulação Neoplásica da Expressão Gênica , Receptores de Hialuronatos/biossíntese , Hialuronoglucosaminidase/biossíntese , Neoplasias Laríngeas/metabolismo , Idoso , Western Blotting , Cartilagem/metabolismo , Movimento Celular , Matriz Extracelular/metabolismo , Humanos , Hialuronoglucosaminidase/antagonistas & inibidores , Masculino , Peso Molecular , RNA/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
The hallmark of cancer invasion is the degradation of extracellular matrix components. Matrix metalloproteinases are the major enzymes participating in this event and their activity is regulated extracellularly by their presence as proenzymes and the concomitant presence of the specific tissue inhibitors. The present study describes the immunohistochemical localization of gelatinases, matrix metalloproteinase (MMP)-2 and -9 and tissue inhibitor of metalloproteinase (TIMP)-1 and -2 in human laryngeal carcinoma and their expression with respect to tumor classification and compared with the respective healthy subjects. MMP-2 was immunolocalized in the cytoplasm of the epithelial cells and in the loose connective tissue, whereas MMP-9 was also observed in basement membrane and chondrocytes. Both were also found in tumor cells, but staining was decreased with increasing stage of cancer. TIMP-1 was present exclusively in stroma and totally absent from tumor cells and it was overexpressed in normal cells surrounding the tumor. TIMP-2 was identified in the cytoplasm of epithelial cells, in stroma and sometimes in chondrocytes. In addition, it was present in tumor cells of only stage IV samples. The expression level of both gelatinases and TIMPs increased as the stage of cancer increased, suggesting the possible post-transcriptional removal of their mRNA. These observations, performed in a given head and neck site, suggest that the behavior of head and neck tumors seems to depend on the site and additional studies should be performed to obtain a general understanding of the disease and ascertain the role of the constituents examined.
Assuntos
Carcinoma de Células Escamosas/enzimologia , Neoplasias Laríngeas/enzimologia , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Inibidor Tecidual de Metaloproteinase-1/metabolismo , Inibidor Tecidual de Metaloproteinase-2/metabolismo , Idoso , Carcinoma de Células Escamosas/genética , Estudos de Casos e Controles , Humanos , Técnicas Imunoenzimáticas , Neoplasias Laríngeas/genética , Laringe/metabolismo , Laringe/patologia , Masculino , Metaloproteinase 2 da Matriz/genética , Metaloproteinase 9 da Matriz/genética , Pessoa de Meia-Idade , Prognóstico , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA Neoplásico/genética , RNA Neoplásico/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Inibidor Tecidual de Metaloproteinase-1/genética , Inibidor Tecidual de Metaloproteinase-2/genéticaRESUMO
Versican and decorin, two proteoglycans (PGs) with contradictory roles in the pathophysiology of cancer, comprise important stromal components in many tumor types and play a crucial role in the progression of cancer. In this study, we provide direct evidence for a significant and stage-related accumulation of versican and decorin in the tumor-associated stroma of laryngeal squamous cell carcinoma (LSCC) in comparison to normal larynx. Both PGs were found to be co-localized within the peritumorous stroma. In addition, the accumulated versican and decorin were markedly modified on both protein core and glycosaminoglycan (GAG) levels. Decorin, which was present under both glycanated and non-glycanated forms, perceptibly increased with the progression of LSCC, compared to the normal larynx. Tumor-associated glycanated decorin was found to contain significant amounts of dermatan sulfate (DS) sequences. Versican was also found to undergo stage-related structural modifications since a marked heterogeneity of protein cores was observed, being intense in late stage of laryngeal cancer. The increased accumulation of both versican and decorin was associated with a significant stage-related increase of the molar ratio of Delta di-mono4S to Delta di-mono6S up to approximately threefold in LSCC compared to the normal ones. The modified chemical structure of both PGs could be associated with the degree of aggressiveness of laryngeal squamous cell carcinomas.
Assuntos
Carcinoma de Células Escamosas/metabolismo , Proteínas da Matriz Extracelular/metabolismo , Neoplasias Laríngeas/metabolismo , Processamento de Proteína Pós-Traducional , Proteoglicanas/metabolismo , Versicanas/metabolismo , Carcinoma de Células Escamosas/patologia , Decorina , Progressão da Doença , Proteínas da Matriz Extracelular/química , Humanos , Neoplasias Laríngeas/química , Neoplasias Laríngeas/patologia , Laringe/metabolismo , Laringe/patologia , Proteoglicanas/química , Regulação para Cima , Ácidos Urônicos/análise , Versicanas/químicaRESUMO
OBJECTIVE: This study was undertaken to investigate the presence of autoantibodies against the main cartilage proteoglycan, aggrecan, in systemic rheumatic disease sera, and to identify substructure(s) responsible for the autoimmune response. METHODS: Sera were obtained from 86 patients with various systemic rheumatic diseases, 14 with osteoarthritis (OA), 18 with cancer and 40 healthy individuals. The presence of autoantibodies against aggrecan was examined by a solid phase assay and by Western blotting, using proteoglycan aggregates treated with proteolytic enzymes. The positive bands were subjected to nanohigh performance liquid chromatography (nanoHPLC)-MS, in order to identify the aggrecan substructures involved in the autoimmune response. RESULTS: Autoantibodies against aggrecan were identified in all systemic rheumatic disease sera at a high titre, almost three times that observed in healthy controls. OA and cancer sera produced a reaction equal to that of the healthy. Western blotting analysis of aggrecan proteolytic fragments revealed the presence of a triple band, reacting with the patients' sera, of about 37 kDa, which also reacted with a polyclonal antibody against hyaluronan-binding region. NanoHPLC-MS analysis suggested that this band belonged to the G2 domain of aggrecan. CONCLUSION: At least a part of the autoimmune reaction to aggrecan, displayed by the systemic disease sera, involves the G2 domain. The significant difference observed between these sera and those from other diseases, especially cancer, may suggest a possible discriminatory role of anti-aggrecan antibodies. This may help in the differential diagnosis in complicated clinical cases. However, for this to be confirmed, studies in larger cohorts of patients should be performed.
Assuntos
Autoanticorpos/sangue , Proteoglicanas de Sulfatos de Condroitina/imunologia , Proteínas da Matriz Extracelular/imunologia , Lectinas Tipo C/imunologia , Doenças Reumáticas/imunologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Agrecanas , Análise de Variância , Western Blotting/métodos , Cromatografia Líquida de Alta Pressão , Ensaio de Imunoadsorção Enzimática , Mapeamento de Epitopos , Humanos , Espectrometria de Massas/métodos , Pessoa de Meia-Idade , Nanotecnologia , Osteoartrite/sangue , Osteoartrite/imunologia , Doenças Reumáticas/sangueRESUMO
Squid cranial cartilage is poor in proteoglycans. They were extracted by 2% SDS and purified by isopycnic centrifugation in the presence of detergent. According to their buoyant density and hydrodynamic size they were fractionated into three structurally different populations of Mr 1.3.10(6), 0.6.10(6) and 1.0.10(6). The proteoglycans of each population differ in the number of oversulphated chondroitin sulphate chains, ranging from two to five, in the number and size of uronic acid and sulphate containing oligosaccharides and in the size of their core protein. The majority, if not all, of the oligosaccharides are linked to the protein via an O-glycosidic bond involving galactosamine and most likely xylose. The chondroitin sulphate chains are segregated on a small peptide segment of the molecule which also contains a large proportion of the oligosaccharides. The proteoglycans have no tendency to interact with hyaluronate.
Assuntos
Cartilagem/metabolismo , Proteoglicanas/isolamento & purificação , Aminoácidos/análise , Animais , Sulfatos de Condroitina/análise , Cromatografia em Gel , Decapodiformes , Oligossacarídeos/análise , Proteoglicanas/análise , Crânio , TripsinaRESUMO
The extractability of squid skin proteoglycans with solutions of varying concentrations of guanidine-HCl, urea and SDS was studied; 4 M guanidine-HCl, being the best extractant, removed 95% of the tissue proteoglycans (glycosaminoglycan uronic acid). The proteoglycans in the 4 M guanidine-HCl extract were fractionated by repeated ion exchange and gel chromatography on Sepharose CL-4B to give three main populations, all being present in about equal proportions. Two populations (Kd 0.34 and 0.56) contained only chondroitin (proteochondroitin) and the other (Kd 0.50) only oversulphated chondroitin sulphate (oversulphated proteochondroitin sulphate). Two minor populations, one containing chondroitin and chondroitin sulphate and the other chondroitin sulphate and oversulphated chondroitin sulphate, were also identified.
Assuntos
Decapodiformes/análise , Proteoglicanas/isolamento & purificação , Pele/análise , Animais , Cromatografia em Gel , Cromatografia Líquida de Alta Pressão , Cromatografia por Troca Iônica , Eletroforese em Acetato de Celulose , Guanidina , Guanidinas , SolventesRESUMO
The effect of cartilage proteoglycans on HA seed crystal growth was studied using a system providing constant supersaturation with respect to HA. The monomers were much less effective than the aggregates in reducing the rate of HA growth, which correlates with their affinity for the HA crystals. Hyaluronan, which is a normal constituent of the proteoglycan aggregates, behaved as a strong inhibitor of HA seed crystal growth and had an affinity constant similar to that of proteoglycan aggregates. The results indicate that inhibition of HA seed crystal growth is mediated through the interaction of hyaluronan with HA crystal surface and that the proteoglycans add to the volume of the adsorbate causing steric hindrance.
Assuntos
Ácido Hialurônico/química , Hidroxiapatitas/química , Proteoglicanas/química , Animais , Cartilagem/química , Galinhas , Cristalização , Proteoglicanas/farmacologia , Propriedades de SuperfícieRESUMO
The composition and the distribution of glycosaminoglycans (GAGs) present in normal human nasal cartilage (HNNC), were examined and compared with those in human scoliotic nasal cartilage (HSNC). In both tissues, hyaluronan (HA), keratan sulfate (KS) and the galactosaminoglycans (GalAGs)--chondroitin sulfate (CS) and dermatan sulfate (DS)--were identified. The overall GAG content in HSNC was approx. 30% higher than the HNNC. Particularly, a 114% increase in HA, and 46% and 86% in KS and DS, respectively, was recorded. CS was the main type of GAG in both tissues with no significant compositional difference. GalAG chains in HSNC exhibited an altered disaccharide composition which was associated with significant increases of non-sulfated and 6-sulfated disaccharides. DS, which was identified and quantitated for the first time in HNNC and HSNC, contained low amounts of iduronic acid (IdoA), 18% and 28% respectively. In contrast to other tissues, where IdoA residues are organized in long IdoA rich repeats, the IdoA residues of DS in human nasal cartilage seemed to be randomly distributed along the chain. DS chains in HSNC were of larger average molecular size than those from HNNC. These results clearly indicate the GAG content and pattern in both HNNC and HSNC and demonstrate that scoliosis of nasal septum cartilage is related to quantitative and structural modifications at the GAG level.
Assuntos
Dermatan Sulfato/análise , Glicosaminoglicanos/química , Septo Nasal/anormalidades , Adulto , Fracionamento Químico , Condroitina ABC Liase , Cromatografia em Gel , Dissacarídeos/análise , Glicosaminoglicanos/isolamento & purificação , Humanos , Peso Molecular , Septo Nasal/química , Septo Nasal/metabolismoRESUMO
The polysaccharides of cranical cartilage were isolated by ethanol precipitation after papain digestion and beta-elimination procedures and were fractionated chromatographically on CPC-cellulose. In addition to the previously described, heavily oversulphated chondroitin sulphate, the tissue contained small amounts of hyaluronic acid, which, however, co-eluted with the chondroitin sulphate from the CPC-cellulose. Approx. 20% of the isolated polysaccharides consisted of an acidic polysaccharide which to our knowledge is not previously described. This polysaccharide consists mainly of glucuronic acid, galactose and mannose in a molar ratio of 1:2:1. Gel chromatography of the preparation indicated a polydisperse molecule with an apparent average molecular weight of 39 200 on weight basis (Mw) and 31 400 on number basis (Mn).
Assuntos
Cartilagem/análise , Decapodiformes/análise , Polissacarídeos/isolamento & purificação , Animais , Cromatografia Líquida de Alta Pressão , Colorimetria , Concentração de Íons de Hidrogênio , Crânio/análiseRESUMO
Bovine pericardium (BP) is a source of natural biomaterials with a wide range of clinical applications. In the present work we studied the dynamic mechanical behavior of BP in native form and under specific enzymatic degradation with chondroitinase ABC extracted a 17% of the total glycosaminoglycans (GAGs). The GAGs content of native BP was composed mainly from hyaluronan, chondroitine sulfate and dermatan sulfate. Dynamic tensile mechanical testing of BP in the frequency range 0.1-20 Hz demonstrated its viscoelastic nature. The storage modulus was equal to 6.5 (native) and 5.5 (degraded) MPa initially, increased in the region nearby 1 Hz by about 15%. This was related with physical resonance mechanisms activated in this frequency region. The high modulus (modulus of the high linear phase of stress-strain) was equal to 14 (native) and 10 (degraded) MPa, dropped at high frequencies to 7 and 5 Mpa, respectively. The damping, expressed by the hysteresis, was equal to 20% of the loading energy, changed exponentially with the frequency to 30% at 20 Hz. It seemed that of the elastic mechanical parameters, the storage modulus and the high modulus were even slightly dropped as a result of degradation. As a final conclusion, there was evident that GAGs may play a non-negligible role in the dynamic mechanical behavior of BP and, probably in other soft tissue biomechanics. It is suggested that the GAGs content may be considered during the design and chemical modification of biomaterials based on BP and other soft tissues.
Assuntos
Glicosaminoglicanos/fisiologia , Pericárdio/fisiologia , Animais , Fenômenos Biomecânicos , Bovinos , Condroitina ABC Liase/metabolismo , Elasticidade , Glicosaminoglicanos/química , Glicosaminoglicanos/isolamento & purificação , Pericárdio/química , Pericárdio/metabolismo , Estresse MecânicoRESUMO
Squid cranial cartilage extracts were found to contain a protein with a molecular mass of 35 kDa immunoreacting with an antiserum against sheep link protein. Because hyaluronan is not detected in this tissue and the structure of proteoglycans is different to that of aggrecan or versican, this observation was studied further. The 35 kDa protein was purified from cartilage extracts and immunolocalised in Western blots by both the polyclonal antibody and the mAb 8A4. It was found that it was able to bind to hyaluronan and to aggrecan. Direct and competitive microplate binding experiments showed that the squid protein binds to G1 domain of aggrecan, similarly to cartilage link protein and, therefore, it could be a link-like protein molecule of squid cranial cartilage. The 35 kDa protein was also able to bind to squid proteoglycan and this suggested that it might participate in squid cartilage proteoglycan aggregate formation.
Assuntos
Cartilagem/química , Proteínas da Matriz Extracelular , Proteínas/química , Agrecanas , Animais , Cromatografia por Troca Iônica , Decapodiformes , Eletroforese em Gel de Poliacrilamida , Ácido Hialurônico/química , Soros Imunes , Lectinas Tipo C , Proteínas/imunologia , Proteínas/isolamento & purificação , Proteoglicanas/químicaRESUMO
In the present work, the interaction of aggrecan, decorin and biglycan isolated from pig laryngeal cartilage and of the three squid cartilage proteoglycans with collagen type I and II was studied. The interaction was examined under conditions allowing the formation of collagen fibrils. It was found that biglycan interacted strongly with collagen type II and not with type I and the interaction seemed to proceed exclusively through its core proteins. Decorin interacted with collagen type I but not with type II. Aggrecan interacted very poorly with both collagen types. The two squid proteoglycans of large size, D1D1A and D1D2, interacted only with collagen type I through both glycosaminoglycans and core proteins. The third squid proteoglycan of small size, D1D1B, interacted poorly only with collagen type I. The results suggested that the interactions of cartilage proteoglycans with collagen were mainly due to the primary structure of both molecules, and would contribute to the maintenance of the integrity of the tissue. The biochemical significance of these interactions might be more critical in aged vertebrate cartilage, where loss of aggrecan and increase of the small proteoglycans was observed, a large proportion of which is found in the extracellular matrix free of glycosaminoglycan chains.
Assuntos
Proteínas da Matriz Extracelular , Cartilagens Laríngeas/química , Proteoglicanas/química , Tendão do Calcâneo/química , Agrecanas , Sequência de Aminoácidos , Animais , Biglicano , Bovinos , Colágeno Tipo I/farmacologia , Colágeno Tipo II/farmacologia , Colagenases/metabolismo , Decapodiformes , Decorina , Técnicas In Vitro , Cinética , Lectinas Tipo C , Ligação Proteica , Proteoglicanas/isolamento & purificação , Proteoglicanas/metabolismo , Suínos , Fator de Crescimento Transformador beta/antagonistas & inibidoresRESUMO
A new type of hyaluronidase was isolated from squid cranial cartilage. The enzyme seems to be localised extracellularly, since it is extracted from the tissue by 0.5 M sodium acetate, pH 7.0, in the presence of proteinase inhibitors. Degradation studies suggest that the enzyme belongs to the family of endoglycosidases generating oligosaccharides of rather large size. The best activity of the enzyme was observed at pH 7.0 and 37 degrees C and the optimum buffer for digestion was 0.15 M Tris acetate. It is inactive in sodium phosphate, morpholine acetate and HEPES buffers. The enzyme degrades aggrecan, hyaluronan, chondroitin sulphate and oversulphated chondroitin sulphate.
Assuntos
Cartilagem/enzimologia , Decapodiformes/enzimologia , Hialuronoglucosaminidase/metabolismo , Crânio/enzimologia , Animais , Sulfatos de Condroitina/metabolismo , Líquido Extracelular/enzimologia , Hialuronoglucosaminidase/análise , Hialuronoglucosaminidase/isolamento & purificaçãoRESUMO
The three populations of squid cranial cartilage proteoglycans, D1D1A, D1D1B and D1D2 appeared to have a high degree of polydispersity. Gel electrophoresis and immunoblotting analysis showed that polydispersity was mainly due to the variable size of chondroitin sulphate E chains. This was further ascertained after rotary shadowing electron microscopy of proteoglycan core proteins and glycosaminoglycan side chains and statistical analysis of the sizes measured for both components. Enzymic treatment of the proteoglycan core proteins produced different peptides from each population, suggesting that the observed heterogeneity of the proteoglycans is due to their core proteins. Antibodies were raised in rabbits against all proteoglycans and enzyme-linked immunosorbent analysis of proteoglycan core proteins revealed that the proteoglycans, even heterogeneous, shared many common epitopes. Part of the common proteoglycan epitopes were found to be located in chondroitin sulphate E chains. Heterogeneity of squid proteoglycans was also investigated by studying their interactions with collagen and it was found that only the two populations of high molecular mass, D1D1A and D1D2, were able to interact with only collagen type I, the latter stronger than the former.
Assuntos
Cartilagem/química , Proteoglicanas/química , Proteoglicanas/ultraestrutura , Animais , Western Blotting , Condroitina ABC Liase , Decapodiformes , Eletroforese em Gel de Ágar , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Microscopia EletrônicaRESUMO
Keratan sulphate was identified in sheep brain. We describe here the isolation and partial characterization of keratan sulphate from cerebrum, cerebellum and brainstem of young sheep brains. The galactosaminoglycan was isolated by using ion-exchange chromatography and gel filtration after exhaustive digestion with papain of the delipidated tissues, followed by alkaline borohydride degradation and chondroitinase ABC and heparinases I, II and III treatment. The material isolated by ion-exchange chromatography from each tissue was eluted as single but polydispersed peak from Sephadex G-75, with average molecular masses 8.4, 7.9 and 8.8 kDa for cerebrum, cerebellum and brainstem, respectively. Keratanase I and II totally degraded keratan sulphate from cerebrum and brainstem, but only partially that from cerebellum. The content of keratan sulphate was found to be about 215, 173 and 144 microg/g dry delipidated tissue for cerebrum, brainstem and cerebellum, respectively.
Assuntos
Tronco Encefálico/química , Cerebelo/química , Glicosídeo Hidrolases , Sulfato de Queratano/isolamento & purificação , Telencéfalo/química , Animais , Sulfato de Queratano/análise , Proteoglicanas/química , Ovinos , beta-Galactosidase/metabolismoRESUMO
Ageing research in Greece is well established. Research groups located in universities, research institutes or public hospitals are studying various and complementary aspects of ageing. These research activities include (a) functional analysis of Clusterin/Apolipoprotein J, studies in healthy centenarians and work on protein degradation and the role of proteasome during senescence at the National Hellenic Research Foundation; (b) regulation of cell proliferation and tissue formation, a nationwide study of determinants and markers of successful ageing in Greek centenarians and studies of histone gene expression and acetylation at the National Center for Scientific Research, Demokritos; (c) work on amyloid precursor protein and Presenilin 1 at the University of Athens; (d) oxidative stress-induced DNA damage and the role of oncogenes in senescence at the University of Ioannina; (e) studies in the connective tissue at the University of Patras; (f) proteomic studies at the Biomedical Sciences Research Center Alexander Fleming; (g) work on Caenorhabditis elegans at the Foundation for Research and Technology; (h) the role of ultraviolet radiation in skin ageing at Andreas Sygros Hospital; (i) follow-up studies in healthy elderly at the Athens Home for the Aged; and (j) socio-cultural aspects of ageing at the National School of Public Health. These research activities are well recognized by the international scientific community as it is evident by the group's very good publication records as well as by their direct funding from both European Union and USA. This article summarizes these research activities and discuss future directions and efforts towards the further development of the ageing field in Greece.