Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 78
Filtrar
1.
Nature ; 597(7877): 539-543, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34526718

RESUMO

Seven years after the declaration of the first epidemic of Ebola virus disease in Guinea, the country faced a new outbreak-between 14 February and 19 June 2021-near the epicentre of the previous epidemic1,2. Here we use next-generation sequencing to generate complete or near-complete genomes of Zaire ebolavirus from samples obtained from 12 different patients. These genomes form a well-supported phylogenetic cluster with genomes from the previous outbreak, which indicates that the new outbreak was not the result of a new spillover event from an animal reservoir. The 2021 lineage shows considerably lower divergence than would be expected during sustained human-to-human transmission, which suggests a persistent infection with reduced replication or a period of latency. The resurgence of Zaire ebolavirus from humans five years after the end of the previous outbreak of Ebola virus disease reinforces the need for long-term medical and social care for patients who survive the disease, to reduce the risk of re-emergence and to prevent further stigmatization.


Assuntos
Surtos de Doenças , Ebolavirus/genética , Ebolavirus/isolamento & purificação , Doença pelo Vírus Ebola/epidemiologia , Doença pelo Vírus Ebola/virologia , Modelos Biológicos , Animais , República Democrática do Congo/epidemiologia , Surtos de Doenças/estatística & dados numéricos , Ebolavirus/classificação , Feminino , Guiné/epidemiologia , Doença pelo Vírus Ebola/transmissão , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Infecção Persistente/virologia , Filogenia , Sobreviventes , Fatores de Tempo , Zoonoses Virais/transmissão , Zoonoses Virais/virologia
2.
N Engl J Med ; 384(13): 1240-1247, 2021 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-33789012

RESUMO

During the 2018-2020 Ebola virus disease (EVD) outbreak in North Kivu province in the Democratic Republic of Congo, EVD was diagnosed in a patient who had received the recombinant vesicular stomatitis virus-based vaccine expressing a ZEBOV glycoprotein (rVSV-ZEBOV) (Merck). His treatment included an Ebola virus (EBOV)-specific monoclonal antibody (mAb114), and he recovered within 14 days. However, 6 months later, he presented again with severe EVD-like illness and EBOV viremia, and he died. We initiated epidemiologic and genomic investigations that showed that the patient had had a relapse of acute EVD that led to a transmission chain resulting in 91 cases across six health zones over 4 months. (Funded by the Bill and Melinda Gates Foundation and others.).


Assuntos
Ebolavirus/genética , Doença pelo Vírus Ebola/transmissão , Adulto , Teorema de Bayes , República Democrática do Congo/epidemiologia , Vacinas contra Ebola/imunologia , Ebolavirus/isolamento & purificação , Evolução Fatal , Genoma Viral , Doença pelo Vírus Ebola/diagnóstico , Doença pelo Vírus Ebola/epidemiologia , Doença pelo Vírus Ebola/terapia , Humanos , Masculino , Mutação , Filogenia , RNA Viral/sangue , Recidiva
3.
Nature ; 546(7658): 401-405, 2017 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-28538723

RESUMO

Zika virus (ZIKV) is causing an unprecedented epidemic linked to severe congenital abnormalities. In July 2016, mosquito-borne ZIKV transmission was reported in the continental United States; since then, hundreds of locally acquired infections have been reported in Florida. To gain insights into the timing, source, and likely route(s) of ZIKV introduction, we tracked the virus from its first detection in Florida by sequencing ZIKV genomes from infected patients and Aedes aegypti mosquitoes. We show that at least 4 introductions, but potentially as many as 40, contributed to the outbreak in Florida and that local transmission is likely to have started in the spring of 2016-several months before its initial detection. By analysing surveillance and genetic data, we show that ZIKV moved among transmission zones in Miami. Our analyses show that most introductions were linked to the Caribbean, a finding corroborated by the high incidence rates and traffic volumes from the region into the Miami area. Our study provides an understanding of how ZIKV initiates transmission in new regions.


Assuntos
Infecção por Zika virus/epidemiologia , Infecção por Zika virus/virologia , Zika virus/genética , Aedes/virologia , Animais , Região do Caribe/epidemiologia , Surtos de Doenças/estatística & dados numéricos , Feminino , Florida/epidemiologia , Genoma Viral/genética , Humanos , Incidência , Epidemiologia Molecular , Mosquitos Vetores/virologia , Zika virus/isolamento & purificação , Infecção por Zika virus/transmissão
4.
Emerg Infect Dis ; 28(6): 1198-1210, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35608626

RESUMO

Knowledge of contemporary genetic composition of dengue virus (DENV) in Africa is lacking. By using next-generation sequencing of samples from the 2017 DENV outbreak in Burkina Faso, we isolated 29 DENV genomes (5 serotype 1, 16 serotype 2 [DENV-2], and 8 serotype 3). Phylogenetic analysis demonstrated the endemic nature of DENV-2 in Burkina Faso. We noted discordant diagnostic results, probably related to genetic divergence between these genomes and the Trioplex PCR. Forward and reverse1 primers had a single mismatch when mapped to the DENV-2 genomes, probably explaining the insensitivity of the molecular test. Although we observed considerable homogeneity between the Dengvaxia and TetraVax-DV-TV003 vaccine strains as well as B cell epitopes compared with these genomes, we noted unique divergence. Continual surveillance of dengue virus in Africa is needed to clarify the ongoing novel evolutionary dynamics of circulating virus populations and support the development of effective diagnostic, therapeutic, and preventive countermeasures.


Assuntos
Vírus da Dengue , Dengue , Burkina Faso/epidemiologia , Dengue/epidemiologia , Surtos de Doenças , Genômica , Humanos , Filogenia , Estudos Retrospectivos , Sorogrupo
5.
Clin Infect Dis ; 70(3): 464-473, 2020 01 16.
Artigo em Inglês | MEDLINE | ID: mdl-30891596

RESUMO

BACKGROUND: Endemic outbreaks of hantaviruses pose a critical public health threat worldwide. Hantaan orthohantavirus (HTNV) causes hemorrhagic fever with renal syndrome (HFRS) in humans. Using comparative genomic analyses of partial and nearly complete sequences of HTNV from humans and rodents, we were able to localize, with limitations, the putative infection locations for HFRS patients. Partial sequences might not reflect precise phylogenetic positions over the whole-genome sequences; finer granularity of rodent sampling reflects more precisely the circulation of strains. METHODS: Five HFRS specimens were collected. Epidemiological surveys were conducted with the patients during hospitalization. We conducted active surveillance at suspected HFRS outbreak areas. We performed multiplex polymerase chain reaction-based next-generation sequencing to obtain the genomic sequence of HTNV from patients and rodents. The phylogeny of human- and rodent-derived HTNV was generated using the maximum likelihood method. For phylogeographic analyses, the tracing of HTNV genomes from HFRS patients was defined on the bases of epidemiological interviews, phylogenetic patterns of the viruses, and geographic locations of HTNV-positive rodents. RESULTS: The phylogeographic analyses demonstrated genetic clusters of HTNV strains from clinical specimens, with HTNV circulating in rodents at suspected sites of patient infections. CONCLUSIONS: This study demonstrates a major shift in molecular epidemiological surveillance of HTNV. Active targeted surveillance was performed at sites of suspected infections, allowing the high-resolution phylogeographic analysis to reveal the site of emergence of HTNV. We posit that this novel approach will make it possible to identify infectious sources, perform disease risk assessment, and implement preparedness against vector-borne viruses.


Assuntos
Vírus Hantaan , Febre Hemorrágica com Síndrome Renal , Orthohantavírus , Orthohantavírus/genética , Febre Hemorrágica com Síndrome Renal/epidemiologia , Humanos , Filogenia , Conduta Expectante
6.
Emerg Infect Dis ; 26(12): 3051-3055, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33219802

RESUMO

We detected Marburg virus RNA in rectal swab samples from Egyptian rousette bats in South Africa in 2017. This finding signifies that fecal contamination of natural bat habitats is a potential source of infection for humans. Identified genetic sequences are closely related to Ravn virus, implying wider distribution of Marburg virus in Africa.


Assuntos
Quirópteros , Doença do Vírus de Marburg , Marburgvirus , Animais , Humanos , Doença do Vírus de Marburg/epidemiologia , Marburgvirus/genética , África do Sul/epidemiologia
8.
Arch Virol ; 165(7): 1715-1717, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32417973

RESUMO

Venezuelan equine encephalitis virus (VEEV) is an important pathogen of medical and veterinary importance in the Americas. In this report, we present the complete genome sequences of five VEEV isolates obtained from pools of Culex (Melanoconion) gnomatos (4) or Culex (Melanoconion) pedroi (1) from Iquitos, Peru. Genetic and phylogenetic analyses showed that all five isolates grouped within the VEEV complex sister to VEEV IIIC and are members of subtype IIID. This is the first report of full-length genomic sequences of VEEV IIID.


Assuntos
Culex/virologia , Vírus da Encefalite Equina Venezuelana/isolamento & purificação , Encefalomielite Equina Venezuelana/virologia , Genoma Viral , Mosquitos Vetores/virologia , Animais , Sequência de Bases , Vírus da Encefalite Equina Venezuelana/classificação , Vírus da Encefalite Equina Venezuelana/genética , Encefalomielite Equina Venezuelana/transmissão , Genômica , Cavalos , Peru , Filogenia
9.
Proc Natl Acad Sci U S A ; 113(48): 13863-13868, 2016 11 29.
Artigo em Inglês | MEDLINE | ID: mdl-27849599

RESUMO

Mosquito-borne flaviviruses, including yellow fever virus (YFV), Zika virus (ZIKV), and West Nile virus (WNV), profoundly affect human health. The successful transmission of these viruses to a human host depends on the pathogen's ability to overcome a potentially sterilizing immune response in the vector mosquito. Similar to other invertebrate animals and plants, the mosquito's RNA silencing pathway comprises its primary antiviral defense. Although a diverse range of plant and insect viruses has been found to encode suppressors of RNA silencing, the mechanisms by which flaviviruses antagonize antiviral small RNA pathways in disease vectors are unknown. Here we describe a viral suppressor of RNA silencing (VSR) encoded by the prototype flavivirus, YFV. We show that the YFV capsid (YFC) protein inhibits RNA silencing in the mosquito Aedes aegypti by interfering with Dicer. This VSR activity appears to be broadly conserved in the C proteins of other medically important flaviviruses, including that of ZIKV. These results suggest that a molecular "arms race" between vector and pathogen underlies the continued existence of flaviviruses in nature.


Assuntos
Proteínas do Capsídeo/genética , Proteínas de Ligação a RNA/genética , Febre Amarela/genética , Vírus da Febre Amarela/genética , Animais , Culicidae/genética , Culicidae/virologia , Vetores de Doenças , Inativação Gênica , Interações Hospedeiro-Patógeno/genética , Humanos , Insetos Vetores/genética , Insetos Vetores/virologia , RNA de Cadeia Dupla/genética , Febre Amarela/transmissão , Febre Amarela/virologia , Vírus da Febre Amarela/patogenicidade
10.
J Infect Dis ; 217(12): 1952-1956, 2018 05 25.
Artigo em Inglês | MEDLINE | ID: mdl-29584885

RESUMO

The use of ribavirin to treat Crimean-Congo hemorrhagic fever virus (CCHFV) infection has been controversial, based on uncertainties about its antiviral efficacy in clinical case studies. We studied the effect of ribavirin treatment on viral populations in a recent case by deep-sequencing analysis of plasma samples obtained from a CCHFV-infected patient before, during, and after a 5-day regimen of ribavirin treatment. The CCHFV load dropped during ribavirin treatment, and subclonal diversity (transitions) and indels increased in viral genomes during treatment. Although the results are based on a single case, these data demonstrate the mutagenic effect of ribavirin on CCHFV in vivo.


Assuntos
Vírus da Febre Hemorrágica da Crimeia-Congo/efeitos dos fármacos , Febre Hemorrágica da Crimeia/tratamento farmacológico , Ribavirina/uso terapêutico , Anticorpos Antivirais/imunologia , Antivirais/imunologia , Vírus da Febre Hemorrágica da Crimeia-Congo/imunologia , Febre Hemorrágica da Crimeia/imunologia , Humanos
11.
Emerg Infect Dis ; 24(6): 1134-1137, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29774854

RESUMO

We detected a high seroprevalence of Marburg virus (MARV) antibodies in fruit bats in South Africa; 19.1% of recaptured bats seroconverted. The MARV RNA isolated closely resembled the 1975 Ozolin strain. These findings indicate endemic MARV circulation in bats in South Africa and should inform policies on MARV disease risk reduction.


Assuntos
Quirópteros/virologia , Reservatórios de Doenças/virologia , Doença do Vírus de Marburg/epidemiologia , Doença do Vírus de Marburg/virologia , Marburgvirus , Animais , Genes Virais , História do Século XXI , Doença do Vírus de Marburg/história , Doença do Vírus de Marburg/transmissão , Marburgvirus/classificação , Marburgvirus/genética , Filogenia , Vigilância em Saúde Pública , Estudos Soroepidemiológicos , África do Sul/epidemiologia
12.
Emerg Infect Dis ; 24(4): 754-757, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29553325

RESUMO

We analyzed whole-genome sequences of 8 enterovirus A71 isolates (EV-A71). We confirm the circulation of genogroup C and the new genogroup E in West Africa. Our analysis demonstrates wide geographic circulation and describes genetic exchanges between EV-A71 and autochthonous EV-A that might contribute to the emergence of pathogenic lineages.


Assuntos
Enterovirus Humano A/classificação , Enterovirus Humano A/genética , Infecções por Enterovirus/epidemiologia , Infecções por Enterovirus/virologia , Variação Genética , Genoma Viral , Genótipo , Humanos , Filogenia , Recombinação Genética
13.
Emerg Infect Dis ; 24(2): 249-257, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29350137

RESUMO

Seoul virus (SEOV) poses a worldwide public health threat. This virus, which is harbored by Rattus norvegicus and R. rattus rats, is the causative agent of hemorrhagic fever with renal syndrome (HFRS) in humans, which has been reported in Asia, Europe, the Americas, and Africa. Defining SEOV genome sequences plays a critical role in development of preventive and therapeutic strategies against the unique worldwide hantavirus. We applied multiplex PCR-based next-generation sequencing to obtain SEOV genome sequences from clinical and reservoir host specimens. Epidemiologic surveillance of R. norvegicus rats in South Korea during 2000-2016 demonstrated that the serologic prevalence of enzootic SEOV infections was not significant on the basis of sex, weight (age), and season. Viral loads of SEOV in rats showed wide dissemination in tissues and dynamic circulation among populations. Phylogenetic analyses showed the global diversity of SEOV and possible genomic configuration of genetic exchanges.


Assuntos
Variação Genética , Febre Hemorrágica com Síndrome Renal/virologia , Reação em Cadeia da Polimerase Multiplex , Vírus Seoul/genética , Animais , Genoma Viral , Saúde Global , Febre Hemorrágica com Síndrome Renal/epidemiologia , Humanos , Filogeografia , RNA Viral/genética , Ratos , República da Coreia/epidemiologia , Estudos Retrospectivos , Estações do Ano , Testes Sorológicos
14.
N Engl J Med ; 373(25): 2448-54, 2015 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-26465384

RESUMO

A suspected case of sexual transmission from a male survivor of Ebola virus disease (EVD) to his female partner (the patient in this report) occurred in Liberia in March 2015. Ebola virus (EBOV) genomes assembled from blood samples from the patient and a semen sample from the survivor were consistent with direct transmission. The genomes shared three substitutions that were absent from all other Western African EBOV sequences and that were distinct from the last documented transmission chain in Liberia before this case. Combined with epidemiologic data, the genomic analysis provides evidence of sexual transmission of EBOV and evidence of the persistence of infective EBOV in semen for 179 days or more after the onset of EVD. (Funded by the Defense Threat Reduction Agency and others.).


Assuntos
Ebolavirus/genética , Doença pelo Vírus Ebola/transmissão , Sêmen/virologia , Adulto , Coito , Ebolavirus/isolamento & purificação , Feminino , Genoma Viral , Doença pelo Vírus Ebola/virologia , Humanos , Libéria , Masculino , RNA Viral/sangue , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sexo sem Proteção
15.
Nucleic Acids Res ; 44(20): 9831-9846, 2016 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-27651462

RESUMO

Ebola virus (EBOV) is a single-stranded negative-sense RNA virus belonging to the Filoviridae family. The leader and trailer non-coding regions of the EBOV genome likely regulate its transcription, replication, and progeny genome packaging. We investigated the cis-acting RNA signals involved in RNA-RNA and RNA-protein interactions that regulate replication of eGFP-encoding EBOV minigenomic RNA and identified heat shock cognate protein family A (HSC70) member 8 (HSPA8) as an EBOV trailer-interacting host protein. Mutational analysis of the trailer HSPA8 binding motif revealed that this interaction is essential for EBOV minigenome replication. Selective 2'-hydroxyl acylation analyzed by primer extension analysis of the secondary structure of the EBOV minigenomic RNA indicates formation of a small stem-loop composed of the HSPA8 motif, a 3' stem-loop (nucleotides 1868-1890) that is similar to a previously identified structure in the replicative intermediate (RI) RNA and a panhandle domain involving a trailer-to-leader interaction. Results of minigenome assays and an EBOV reverse genetic system rescue support a role for both the panhandle domain and HSPA8 motif 1 in virus replication.


Assuntos
Ebolavirus/genética , Genoma Viral , Proteínas de Choque Térmico/metabolismo , Interações Hospedeiro-Patógeno , Conformação de Ácido Nucleico , RNA Viral/química , RNA Viral/genética , Proteínas de Choque Térmico HSC70/metabolismo , Proteínas de Choque Térmico/química , Proteínas de Choque Térmico/genética , Doença pelo Vírus Ebola/metabolismo , Doença pelo Vírus Ebola/virologia , Humanos , Modelos Moleculares , Mutação , Motivos de Nucleotídeos , Ligação Proteica , Interferência de RNA , RNA Interferente Pequeno , Transcrição Gênica , Replicação Viral
16.
Emerg Infect Dis ; 23(8): 1274-1281, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-28548637

RESUMO

Unprotected sexual intercourse between persons residing in or traveling from regions with Zika virus transmission is a risk factor for infection. To model risk for infection after sexual intercourse, we inoculated rhesus and cynomolgus macaques with Zika virus by intravaginal or intrarectal routes. In macaques inoculated intravaginally, we detected viremia and virus RNA in 50% of macaques, followed by seroconversion. In macaques inoculated intrarectally, we detected viremia, virus RNA, or both, in 100% of both species, followed by seroconversion. The magnitude and duration of infectious virus in the blood of macaques suggest humans infected with Zika virus through sexual transmission will likely generate viremias sufficient to infect competent mosquito vectors. Our results indicate that transmission of Zika virus by sexual intercourse might serve as a virus maintenance mechanism in the absence of mosquito-to-human transmission and could increase the probability of establishment and spread of Zika virus in regions where this virus is not present.


Assuntos
Macaca fascicularis , Macaca mulatta , Infecção por Zika virus/virologia , Zika virus/fisiologia , Animais , Feminino , Masculino , Vagina , Replicação Viral , Eliminação de Partículas Virais , Infecção por Zika virus/transmissão
17.
J Gen Virol ; 98(5): 935-945, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28488954

RESUMO

The Bunyaviridae family comprises viruses causing diseases of public and veterinary health importance, including viral haemorrhagic and arboviral fevers. We report the isolation, identification and genome characterization of a novel orthobunyavirus, named Wolkberg virus (WBV), from wingless bat fly ectoparasites (Eucampsipoda africana) of Egyptian fruit bats (Rousettus aegyptiacus) in South Africa. Complete genome sequence data of WBV suggests it is most closely related to two bat viruses (Mojuí dos Campos and Kaeng Khoi viruses) and an arbovirus (Nyando virus) previously shown to infect humans. WBV replicates to high titres in VeroE6 and C6-36 cells, characteristic of mosquito-borne arboviruses. These findings expand our knowledge of the diversity of orthobunyaviruses and their insect vector host range.


Assuntos
Quirópteros/parasitologia , Dípteros/virologia , Orthobunyavirus/classificação , Orthobunyavirus/isolamento & purificação , Animais , Linhagem Celular , Análise por Conglomerados , Genoma Viral , Microscopia Eletrônica de Transmissão , Orthobunyavirus/genética , Filogenia , Análise de Sequência de DNA , Homologia de Sequência , África do Sul , Vírion/ultraestrutura , Cultura de Vírus
18.
BMC Bioinformatics ; 16: 416, 2015 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-26714571

RESUMO

BACKGROUND: The detection of pathogens in complex sample backgrounds has been revolutionized by wide access to next-generation sequencing (NGS) platforms. However, analytical methods to support NGS platforms are not as uniformly available. Pathosphere (found at Pathosphere.org) is a cloud - based open - sourced community tool that allows for communication, collaboration and sharing of NGS analytical tools and data amongst scientists working in academia, industry and government. The architecture allows for users to upload data and run available bioinformatics pipelines without the need for onsite processing hardware or technical support. RESULTS: The pathogen detection capabilities hosted on Pathosphere were tested by analyzing pathogen-containing samples sequenced by NGS with both spiked human samples as well as human and zoonotic host backgrounds. Pathosphere analytical pipelines developed by Edgewood Chemical Biological Center (ECBC) identified spiked pathogens within a common sample analyzed by 454, Ion Torrent, and Illumina sequencing platforms. ECBC pipelines also correctly identified pathogens in human samples containing arenavirus in addition to animal samples containing flavivirus and coronavirus. These analytical methods were limited in the detection of sequences with limited homology to previous annotations within NCBI databases, such as parvovirus. Utilizing the pipeline-hosting adaptability of Pathosphere, the analytical suite was supplemented by analytical pipelines designed by the United States Army Medical Research Insititute of Infectious Diseases and Walter Reed Army Institute of Research (USAMRIID-WRAIR). These pipelines were implemented and detected parvovirus sequence in the sample that the ECBC iterative analysis previously failed to identify. CONCLUSIONS: By accurately detecting pathogens in a variety of samples, this work demonstrates the utility of Pathosphere and provides a platform for utilizing, modifying and creating pipelines for a variety of NGS technologies developed to detect pathogens in complex sample backgrounds. These results serve as an exhibition for the existing pipelines and web-based interface of Pathosphere as well as the plug-in adaptability that allows for integration of newer NGS analytical software as it becomes available.


Assuntos
Interface Usuário-Computador , Algoritmos , Animais , Arenavirus/genética , Arenavirus/isolamento & purificação , Biologia Computacional , Coronavirus/genética , Coronavirus/isolamento & purificação , Bases de Dados Factuais , Flavivirus/genética , Flavivirus/isolamento & purificação , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Internet , RNA Viral/química , RNA Viral/metabolismo , Análise de Sequência de RNA
19.
Emerg Infect Dis ; 21(7): 1135-43, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-26079255

RESUMO

To support Liberia's response to the ongoing Ebola virus (EBOV) disease epidemic in Western Africa, we established in-country advanced genomic capabilities to monitor EBOV evolution. Twenty-five EBOV genomes were sequenced at the Liberian Institute for Biomedical Research, which provided an in-depth view of EBOV diversity in Liberia during September 2014-February 2015. These sequences were consistent with a single virus introduction to Liberia; however, shared ancestry with isolates from Mali indicated at least 1 additional instance of movement into or out of Liberia. The pace of change is generally consistent with previous estimates of mutation rate. We observed 23 nonsynonymous mutations and 1 nonsense mutation. Six of these changes are within known binding sites for sequence-based EBOV medical countermeasures; however, the diagnostic and therapeutic impact of EBOV evolution within Liberia appears to be low.


Assuntos
Ebolavirus/genética , Doença pelo Vírus Ebola/virologia , Antivirais/farmacologia , Antivirais/uso terapêutico , Análise Mutacional de DNA , Farmacorresistência Viral/genética , Evolução Molecular , Genes Virais , Doença pelo Vírus Ebola/tratamento farmacológico , Doença pelo Vírus Ebola/epidemiologia , Humanos , Libéria/epidemiologia
20.
J Gen Virol ; 96(8): 2079-2085, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25934793

RESUMO

Punta Toro virus (PTV), a member of the PTV complex, is a relatively common causative agent of febrile illness in Panama that is often misdiagnosed as 'dengue' or 'influenza'. Currently, only two named members make up this species complex, PTV and Buenaventura virus (BUEV). Genomic and antigenic characterization of 17 members of the PTV complex, nine of which were isolated from human acute febrile illness cases, reveals that this species complex is composed of six distant viruses. We propose to add four additional new viruses, designated Leticia virus, Cocle virus, Campana virus and Capira virus.


Assuntos
Infecções por Bunyaviridae/virologia , Febre/virologia , Phlebovirus/isolamento & purificação , Animais , Anticorpos Antivirais , Infecções por Bunyaviridae/imunologia , Reações Cruzadas , Febre/imunologia , Humanos , Insetos Vetores/virologia , Dados de Sequência Molecular , Panamá , Phlebovirus/classificação , Phlebovirus/genética , Phlebovirus/imunologia , Filogenia , Psychodidae/virologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA