Larval exposure to environmentally relevant concentrations of triclosan impairs metamorphosis and reproductive fitness in zebrafish.

Developmental exposure to endocrine disruptors can cause organizational changes resulting in latent and transgenerational disease. We exposed zebrafish to environmentally relevant concentrations of triclosan during the critical period of metamorphosis and somatic sex differentiation to determine effects on metamorphosis and reproduction. We use biological and morphological biomarkers to predict potential modes of action. Larval exposure to environmentally relevant concentrations of triclosan was sufficient to cause adverse effects in adults and their offspring. TCS exposure delays metamorphosis and impairs fecundity and fertility. Offspring from TCS-exposed fish show decreased survival and delayed maturation, but their reproductive capacity is not altered. Delays in metamorphosis in conjunction with morphological indicators suggest that toxicity may result from lowered thyroid hormones in parental fish. This work illustrates the importance of evaluating the latent effects of early exposure to environmental contaminants, and that further studies to evaluate the effects of triclosan on the thyroid axis are warranted.

Embryonic exposure to environmentally relevant concentrations of triclosan impairs foraging efficiency in zebrafish larvae.

The ubiquitous and persistent contaminant triclosan is known to cause developmental and behavioral toxicity in fish, but few studies have evaluated the long-term effects of these responses. We used a phenotypically anchored approach to evaluate the behavioral responses caused by early exposure to environmentally relevant concentrations of triclosan to better understand the risk triclosan poses to fish. Zebrafish were exposed to 0, 0.4, 4, or 40 µg triclosan/L (nominal concentrations) for 5 d followed by depuration for 16 d to assess effects on mortality, development, and foraging efficiency. Because foraging efficiency can be impacted by neurological and structural alterations, we assessed morphological and behavioral indicators of neurotoxicity and morphology of craniofacial features associated with gape to identify potential underlying mechanisms associated with altered foraging behaviors. To our knowledge, we are the first to show that early exposure to environmentally relevant concentrations of triclosan impairs foraging efficiency in larval fish by 10%, leading to emaciation and reduced growth and survival. The cause of the impacts of triclosan on foraging efficiency remains unknown, because effects were not associated with overt indicators of neurotoxicity or grossly malformed craniofacial structures. Our results suggest that early exposure to triclosan has the potential to impact the sustainability of wild fish populations, and thus the mechanism underlying behavioral alterations following exposure to triclosan warrants further study. Environ Toxicol Chem 2018;37:3124-3133. © 2018 SETAC.