Virome of wild rats (Rattus norvegicus) captured far from pig farms in Jiangsu province of China reveals novel porcine circovirus type 2d (PCV2d) sequences.

BACKGROUND: Porcine circovirus type 2 (PCV2) has caused great economic losses in the global pig industry. There have been published records of wild rats acting as the reservoirs of PCV2 (only PCV2a and PCV2b), but almost all of which were related to the PCV2-infected swine herds. RESULTS: In this study, we carried out the detection, amplification, and characterization of novel PCV2 strains in wild rats that were captured far from pig farms. Nested PCR assay demonstrated that the kidney, heart, lung, liver, pancreas, and large and small intestines of rats were screened positive for PCV2. We subsequently sequenced two full genomes of PCV2 in positive sample pools, designated as js2021-Rt001 and js2021-Rt002. Genome sequence analysis indicated that they had the highest similarity to nucleotide sequences of porcine-origin PCV2 isolates in Vietnam. Phylogenetically, js2021-Rt001 and js2021-Rt002 were a part of the PCV2d genotype cluster, which is a predominant genotype circulating worldwide in recent years. The antibody recognition regions, immunodominant decoy epitope, and heparin sulfate binding motif of the two complete genome sequences coincided with those previously reported. CONCLUSIONS: Our research reported the genomic characterization of two novel PCV2 strains (js2021-Rt001 and js2021-Rt002) and provided the first supported evidence that PCV2d could naturally infect wild rats in China. However, whether the newly identified strains have potential for circulating in nature in vertical and horizontal transmission or inter-species jumping between rats and pigs needs further research.

Scaling relations reveal global and regional differences in morphometry of reservoirs and natural lakes.

Water bodies provide essential ecosystem services linked to morphometric features that might differ between natural lakes and reservoirs. We use the HydroLAKES global dataset to quantitatively compare large (area > 1 km2) reservoirs and natural lakes in terms of scaling exponents between morphometric measures (volume, area, shore length). These exponents are further compared to those expected from geometrical assumptions and constraints. Lakes cover a larger range of volumes for the same range of surface areas than reservoirs, and have a larger volume-area scaling exponent. The volume-area scaling exponent for reservoirs (but not natural lakes) and the area-shore length exponent for all water bodies follow the predictions for self-affine surfaces. Land cover and terrain influence the scaling relations more for lakes than for reservoirs. These morphometric differences may be used to model the impact of reservoirs and lakes on hydrological processes and associated ecosystem services at regional to global scales.