RESUMO
BACKGROUND: Previous clinical trials have not proved that adding epidermal growth factor receptor inhibitors to chemotherapy confers a survival benefit for patients with advanced biliary tract cancer (ABTC). Whether the KRAS mutation status of tumor cells confounded the results of past studies is unknown. PATIENTS AND METHODS: ABTC patients stratified by KRAS status, Eastern Cooperative Oncology Group performance status, and primary tumor location were randomized 1 : 1 to receive GEMOX (800 mg/m(2) gemcitabine and 85 mg/m(2) oxaliplatin) or C-GEMOX (500 mg/m(2) cetuximab plus GEMOX) every 2 weeks. The primary end point was objective response rate (ORR). RESULTS: The study enrolled 122 patients between December 2010 and May 2012 (62 treated with C-GEMOX and 60 with GEMOX). Compared with GEMOX alone, C-GEMOX was associated with trend to better ORR (27% versus 15%; P = 0.12) and progression-free survival (PFS, 6.7 versus 4.1 months; P = 0.05), but not overall survival (OS, 10.6 versus 9.8 months; P = 0.91). KRAS mutations, which were detected in 36% of tumor samples, did not affect the trends of difference in ORR and PFS between C-GEMOX and GEMOX. The two treatment arms had similar adverse events, except that more patients had skin rashes, allergic reactions, and neutropenia in the C-GEMOX arm. Of patients with C-GEMOX, the presence of a grade 2 or 3 skin rash was associated with significantly better ORR, PFS, and OS. CONCLUSIONS: Addition of cetuximab did not significantly improve the ORR of GEMOX chemotherapy in ABTC, although a trend of PFS improvement was observed. The trend of improvement did not correlate with KRAS mutation status. CLINICAL TRIALS NUMBER: This study is registered at ClinicalTrials.gov (NCT01267344). All patients gave written informed consent.
Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Neoplasias do Sistema Biliar/tratamento farmacológico , Cetuximab/administração & dosagem , Desoxicitidina/análogos & derivados , Mutação , Proteínas Proto-Oncogênicas p21(ras)/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Neoplasias do Sistema Biliar/genética , Neoplasias do Sistema Biliar/mortalidade , Neoplasias do Sistema Biliar/patologia , Cetuximab/efeitos adversos , Desoxicitidina/efeitos adversos , Desoxicitidina/uso terapêutico , Progressão da Doença , Intervalo Livre de Doença , Esquema de Medicação , Feminino , Predisposição Genética para Doença , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Compostos Organoplatínicos/efeitos adversos , Compostos Organoplatínicos/uso terapêutico , Fenótipo , Modelos de Riscos Proporcionais , Taiwan , Fatores de Tempo , Resultado do TratamentoAssuntos
Obstrução Nasal/diagnóstico por imagem , Septo Nasal/diagnóstico por imagem , Septo Nasal/patologia , Tomografia Computadorizada por Raios X , Conchas Nasais/diagnóstico por imagem , Conchas Nasais/patologia , Adolescente , Adulto , Estudos de Coortes , Feminino , Humanos , Hipertrofia , Masculino , Pessoa de Meia-Idade , Obstrução Nasal/etiologia , Obstrução Nasal/patologia , Adulto JovemRESUMO
1. Minocycline, memantine,and glycoconjugate were assessed for their ability to protect cultured primary cortical neurons against double-stranded RNA-induced neurotoxicity. 2. Minocycline but not memantine or glycoconjugate protected cultured cells and warrants further investigation.
Assuntos
Antibacterianos/farmacologia , Minociclina/farmacologia , Fármacos Neuroprotetores/farmacologia , Síndromes Neurotóxicas/prevenção & controle , Análise de Variância , Apoptose , Caspase 3/metabolismo , Linhagem Celular Tumoral , Encefalite Japonesa/complicações , Antagonistas de Aminoácidos Excitatórios/farmacologia , Glicoconjugados/farmacologia , Humanos , L-Lactato Desidrogenase/metabolismo , Memantina/farmacologia , Neurônios/efeitos dos fármacos , Neurônios/enzimologia , Neurônios/patologia , Síndromes Neurotóxicas/enzimologia , Síndromes Neurotóxicas/etiologia , RNA de Cadeia DuplaAssuntos
Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/terapia , Neoplasias do Seio Maxilar/patologia , Neoplasias do Seio Maxilar/terapia , Papiloma Invertido/patologia , Papiloma Invertido/terapia , Idoso , Carcinoma de Células Escamosas/mortalidade , Feminino , Humanos , Masculino , Neoplasias do Seio Maxilar/mortalidade , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Papiloma Invertido/mortalidade , Estudos Retrospectivos , Taxa de Sobrevida , Resultado do TratamentoRESUMO
Hepatic estrogen receptors (ERs) of the female turtle, Chrysemys picta, when complexed with [3H]estradiol ([3H]E2), were shown to bind specifically to liver chromatin isolated from the same species. The binding of the [3H]E2 receptor complex to chromatin requires both the steroid ligand and the receptor protein. Maximal binding occurred within 60-70 min of incubation at 4 degrees C in a Tris buffer containing 0.1 M KCl. The binding of the [3H]E2 receptor complex to intact chromatin was saturable, whereas the binding to turtle or calf thymus DNA remained linear. Scatchard analyses revealed more estrogen receptor binding sites on hepatic chromatin isolated from female turtles than that prepared from the males (binding capacities: female chromatin = 67.9 +/- 6.8 fmol/mg DNA equivalent; male chromatin = 28.5 +/- 2.5 fmol/mg DNA equivalent). Furthermore, the [3H]E2 receptor complex was bound with a higher affinity to female chromatin than to male chromatin (association constants: female chromatin = 11.7 +/- 2.7 X 10(10) M-1; male chromatin = 2.5 +/- 0.7 X 10(10) M-1). In contrast to turtle hepatic [3H]E2 receptors, ERs in rat liver or mouse uterine cytosol exhibited little binding affinity for hepatic chromatin isolated from the turtle. Tissue specificity was demonstrated in the interaction of the [3H]E2 receptor complex and chromatin; high affinity, saturable binding of the [3H]E2 receptor complex was only observed on chromatin isolated from the liver but not on those prepared from the heart, kidney and muscle. A 3- to 4-fold increase in the number of hepatic chromatin [3H]E2 receptor binding sites was observed in 21-day ovariectomized or hypophysectomized female (capacities = 209.3 +/- 6.1 and 270 +/- 10.1 fmol/mg DNA equivalent, respectively). It is postulated that [3H]E2 receptor binding sites on the chromatin of intact females are partially 'masked', and removal of a gonadal and/or pituitary factor(s) unveils additional binding sites on the female chromatin. This paper is first to report the presence of high affinity, species- and tissue-specific acceptor sites on the liver chromatin of a reptilian species. The fact that the levels and properties of these acceptor sites are dependent on the sex and hormonal state of the animal suggests that they may play a role in the regulation of hepatic estrogen responsiveness and vitellogenesis in this species.
Assuntos
Núcleo Celular/metabolismo , Estradiol/farmacologia , Fígado/metabolismo , Receptores de Estrogênio/metabolismo , Animais , Sítios de Ligação/efeitos dos fármacos , Bovinos , Cromatina/metabolismo , DNA/metabolismo , Humanos , Rim/metabolismo , Masculino , Músculos/metabolismo , Miocárdio/metabolismo , Ratos , Fatores Sexuais , Especificidade da Espécie , Timo/metabolismo , TartarugasRESUMO
Our objective was to investigate whether notification of high-risk status for type 1 diabetes in newborn infants results in an increased maternal-parenting stress level when compared with notification of low-risk status for type 1 diabetes. Maternal parenting stress level was assessed at 5-7 weeks postpartum (baseline) and was reassessed 4-5 months after parents were informed of their newborn infants' genetic screening results (follow-up). Parenting stress level was measured using the total stress score (TSS) of the Parenting Stress Index/Short Form. The outcome variable, change in TSS, was calculated by subtracting the baseline TSS from the follow-up TSS. Demographic variables such as maternal race, maternal age, maternal education level, maternal marital status, child's birth order, and total family income were assessed through a structured phone interview at the time of baseline assessment. The risk factor of interest was the child's human leukocyte antigen (HLA) status for type 1 diabetes, i.e., whether child was at a high or moderate (combined into "high") genetic risk or at a low genetic risk for type 1 diabetes. A sample of 88 mothers (23 with a high-risk child and 65 with a low-risk child) was evaluated. Baseline median TSSs were 65 and 74 for mothers of low-risk infants and mothers of high-risk infants, respectively. Both groups' median TSS decreased between baseline and follow-up. No significant differences were found between change in TSS and maternal age, race, education level, marital status, total family income, or child's birth order. Although the median decrease in TSS was smaller in mothers with a high-risk child when compared with mothers of a low-risk child, this difference was not statistically significant. We did not find an association between newborn's HLA status and change in maternal TSS. The results of this study suggest that notification of high-risk status for type 1 diabetes in newborn infants may not result in an increased level of parenting stress among mothers.
Assuntos
Diabetes Mellitus Tipo 1/genética , Diabetes Mellitus Tipo 1/psicologia , Testes Genéticos , Comportamento Materno , Estresse Psicológico , Adulto , Diabetes Mellitus Tipo 1/imunologia , Feminino , Seguimentos , Aconselhamento Genético , Antígenos HLA/genética , Humanos , Lactente , Recém-Nascido , Masculino , Fatores de Risco , Inquéritos e QuestionáriosRESUMO
The efficacy of ciprofloxacin as antibacterial prophylaxis for allogeneic bone marrow transplantation has been well documented, and it virtually eliminated bacteremias caused by gram-negative pathogens in early reports. Ciprofloxacin was therefore incorporated into the prophylactic antibiotic regimen during allogeneic bone marrow or peripheral blood stem cell transplantation at Veterans General Hospital, Kaohsiung from February 1997. In 12 consecutive patients receiving allogeneic bone marrow or peripheral blood stem cell transplantation, ciprofloxacin-resistant Escherichia coli bacteremia developed in three (25%). In addition to our data, increasing evidence suggests that the widespread use of a fluoroquinolone is associated with the emergence of resistant isolates as well as documented infections caused by these resistant strains. The incidence of Escherichia coli bacteremia in our transplant patients was 25%, which was similar to that in patients not receiving preventive therapy or in those receiving trimethoprim-sulfamethoxazole prophylaxis. The prophylactic efficacy of ciprofloxacin in allogeneic bone marrow transplant or peripheral blood stem cell transplant recipients should therefore be reassessed.
Assuntos
Ciprofloxacina/administração & dosagem , Administração Oral , Adolescente , Adulto , Anti-Infecciosos/uso terapêutico , Transplante de Medula Óssea , Resistência Microbiana a Medicamentos , Infecções por Escherichia coli/tratamento farmacológico , Infecções por Escherichia coli/prevenção & controle , Febre , Humanos , Pessoa de Meia-Idade , Taxa de Sobrevida , Transplante Homólogo , Resultado do TratamentoRESUMO
This paper presents results for the calculation of particle trajectories in a cascade and a rocket nozzle using a Lagrangian method. When the floating particles collide to the components, the component surface is damaged severely. The surface erosion rate is strongly dependent on a particle size, a particle impact angle and a surface material. For a compressor cascade, the particle impact rate increases proportionally with the flow inlet angle and the erosion rate on the pressure side surface of blade are related to the surface or coating materials. For a solid rocket nozzle, the particle free zone in the nozzle divergent section increases quickly with increasing particle size and the maximum heat transfer density occurs at the starting region of nozzle convergent section. The Al2O3 droplet breaks up around the nozzle throat due to the high velocity difference between the droplet and gas stream, resulting in the big change of particle free zone.
RESUMO
An immunoperoxidase histochemical technique utilizing a monoclonal primary antibody was developed for detection of Newcastle disease virus (NDV) antigen in tissues from chickens. The technique was applied to trachea, lung, spleen, Harderian gland, and cecal tonsil harvested from specific-pathogen-free (SPF) chickens at 2, 5, 7, 10, and 14 days postinoculation (PI) with NDV, and to corresponding tissues from commercial broiler chickens representing 30 cases of spontaneous respiratory disease. Positive staining occurred in the cytoplasm of respiratory epithelial cells in the trachea or bronchi of NDV-inoculated SPF chickens at 5 and 7 days PI. Staining also occurred in the respiratory epithelium of the trachea and bronchi of commercial broilers from seven of 30 cases of spontaneous respiratory disease. These results indicate that the immunoperoxidase technique has value as a rapid diagnostic test for Newcastle disease.
Assuntos
Galinhas/microbiologia , Doença de Newcastle/microbiologia , Vírus da Doença de Newcastle/isolamento & purificação , Animais , Antígenos Virais/análise , Técnicas Imunoenzimáticas/veterinária , Pulmão/imunologia , Vírus da Doença de Newcastle/imunologia , Organismos Livres de Patógenos Específicos , Traqueia/imunologiaRESUMO
In this paper, we report a case where a bladder perforation occurred during a laparoscopically assisted vaginal hysterectomy and was repaired by laparoscopic loop ligatures. This is the first case report of using the laparoscopic loop ligatures to close the bladder perforations. The loop ligature is an easy and quick procedure, which can be performed by most surgeons who take the time to learn the endoscopic suturing techniques.
Assuntos
Histerectomia Vaginal , Complicações Intraoperatórias/cirurgia , Laparoscopia/métodos , Técnicas de Sutura , Bexiga Urinária/lesões , Feminino , Humanos , Histerectomia Vaginal/métodos , Pessoa de Meia-Idade , Bexiga Urinária/cirurgia , Neoplasias do Colo do Útero/cirurgiaRESUMO
An angiogenic factor from human transitional cell cancer of bladder has been purified by protein extraction, cation exchange chromatography, gel filtration high-performance liquid chromatography (GE-HPLC) and reversed-phase high-performance liquid chromatography (RP-HPLC). The purified substance was named bladder cancer angiogenic factor (BCAF). Biological activity of the BCAF was assessed by the method of chick embryo chorioallantoic membrane (CAM) assay and 3H-TdR incorporation into DNA in Balb/C 3T3 cells. The BCAF displayed the potent activities of neovascularization in CAM and DNA synthesis in Balb/C 3T3 cells. The ultrastructural features of blood vessels induced by the BCAF were similar to the blood vessels in tumors. The BCAF contained a protein with an approximate molecular weight of 15,000 D which was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining. Amino acid compositions of the BCAF were also analysed by acid hydrolysis.
Assuntos
Indutores da Angiogênese/isolamento & purificação , Carcinoma de Células de Transição/química , Neoplasias da Bexiga Urinária/química , Células 3T3 , Indutores da Angiogênese/farmacologia , Animais , Embrião de Galinha , DNA/biossíntese , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Neovascularização PatológicaRESUMO
Hereditary nonpolyposis colon cancer (HNPCC), also known as Lynch syndrome, is characterized by germline and somatic mutations of DNA mismatch repair genes with dominant inheritance of site-specific colorectal cancer or colorectal cancer plus cancers of extracolonic sites. We describe two Taiwanese HNPCC families with members who had predominantly gynecologic malignancies. In one family, the 53-year-old proband was found to have five synchronous and metachronous tumors of the genitourinary system, which included endometrial adenocarcinoma, cervical squamous cell carcinoma, ureteral and bladder transitional cell carcinoma, and ovarian teratoma. Fourteen of her first- and second-degree relatives were victims of genitourinary and gastrointestinal malignancies. The other family was characterized by four sisters who developed endometrial adenocarcinomas at young ages (36-42 yr). Their father died of both stomach cancer and colon cancer at age 47. The diagnosis of HNPCC was confirmed in this family by genetic analysis. A heterozygous germline mutation (G5 to G6 frame-shift at 183-187) of the hMSH2 (human MutS homolog 2) gene was identified in white blood cells of all the affected family members. The frequent presentation of genitourinary cancers in HNPCC highlights the importance of family-history taking in patients with gynecologic cancers and a genetic diagnosis of HNPCC.
Assuntos
Neoplasias Colorretais Hereditárias sem Polipose/genética , Proteínas de Ligação a DNA , Neoplasias dos Genitais Femininos/genética , Adulto , Feminino , Humanos , Pessoa de Meia-Idade , Proteína 2 Homóloga a MutS , Mutação , Proteínas Proto-Oncogênicas/genéticaRESUMO
A simple method for the identification of mutational sites in human mitochondrial DNA (mtDNA) was described. It was based on the human Cambridge sequence as a relative standard sequence and a single base pair substitution in mtDNA as a unique mutational form. The partial mutational sites can be determined using this method which was characterized by combining the restriction mapping with the analysis for the table of human mtDNA potential mutational sites with rapidity and simplicity. In the meanwhile, six mtDNA mutational sites found in Chinese population were identified by means of this method.
Assuntos
DNA Mitocondrial/genética , Polimorfismo de Fragmento de Restrição , Mapeamento por Restrição , China , Análise Mutacional de DNA , Desoxirribonucleases de Sítio Específico do Tipo II/genética , Feminino , Humanos , Grupos MinoritáriosRESUMO
Primary intraosseous venous malformations are rare benign tumors that account for approximately 1% of all primary osseous tumors. They are rarely found in the midface. The authors report a case of an intraosseous venous malformation in a 28-year-old woman who presented with a bony lesion in the nasal bone. Treatment involved surgical excision via open rhinoplasty. Histopathology indicated an intraosseous venous malformation. 16 months postoperatively, there was no evidence of recurrence, the functional and cosmetic results were good, and the patient was satisfied with the treatment outcome.
Assuntos
Osso Nasal/irrigação sanguínea , Procedimentos de Cirurgia Plástica/métodos , Malformações Vasculares/cirurgia , Adulto , Cartilagem/transplante , Estética , Fascia Lata/transplante , Feminino , Humanos , Osteotomia , Satisfação do Paciente , Rinoplastia/métodos , Retalhos Cirúrgicos , Tomografia Computadorizada por Raios X , Resultado do TratamentoRESUMO
The current treatment for metastatic gastric cancer (MGC) consists of cisplatin and/or fluorouracil (5-FU) based combination chemotherapy, but cisplatin-based regimens are associated with considerable toxicity. We evaluated the efficacy and safety of a noncisplatin-, non-5-FU-containing regimen, docetaxel/irinotecan in MGC. Chemo-naive patients with MGC received docetaxel (30 mg m(-2)) and irinotecan (70 mg m(-2)) on days 1 and 8 every 3 weeks. The 48 eligible patients (median age 56 years) received a median of four cycles of docetaxel/irinotecan (range 1-18). Of the 46 patients in whom efficacy could be evaluated, 21 showed a partial response (response rate=45.7%; 95% confidence interval (CI) 31.3-60.1%). At a median follow-up of 15.0 months, the median time to progression was 4.5 months (95% CI 3.8-5.2 months) and overall survival was 8.2 months (95% CI, 5.8-10.6 months). Grade 3/4 neutropenia developed in 57.4% of patients, and febrile neutropenia/neutropenic infection in 19.1%. Nonhaematological toxicities were moderate; grade 3/4 diarrhoea occurred in 19.1% of patients, however, was manageable by a dose reduction. There was one possible treatment-related death. In conclusion, weekly docetaxel/irinotecan is a promising outpatient regimen in MGC, with appropriate dose modification.
Assuntos
Adenocarcinoma/tratamento farmacológico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Ovarianas/tratamento farmacológico , Neoplasias Peritoneais/tratamento farmacológico , Neoplasias Gástricas/tratamento farmacológico , Adenocarcinoma/secundário , Adulto , Idoso , Camptotecina/administração & dosagem , Camptotecina/análogos & derivados , Docetaxel , Relação Dose-Resposta a Droga , Feminino , Humanos , Irinotecano , Neoplasias Hepáticas/secundário , Masculino , Pessoa de Meia-Idade , Neoplasias Ovarianas/secundário , Neoplasias Peritoneais/secundário , Neoplasias Gástricas/patologia , Taxa de Sobrevida , Taxoides/administração & dosagemRESUMO
The posterior interosseous artery (PIA) is the nutritive artery of the dorsal forearm flap. This article describes the dissection study for PIA in 40 cases. The results are as follows: 1. PIA is classified into different types; 2. the external diameter of PIA at the inferior margin of the supinator, the length of PIA and the distance between the artery and external epicondyle of the humerus are described; 3. the course of the artery and its neighboring structures are defined. The above study provides the foundation of designing of the posterior forearm pedicled flap in the repair of defects situated on the forearm and hand.
Assuntos
Antebraço/irrigação sanguínea , Adulto , Artérias/anatomia & histologia , Cadáver , HumanosRESUMO
The present study describes the seasonal changes of the estrogen receptor (ER) system in the liver of the turtle, Chrysemys picta. [3H]Estradiol ([3H]E2) binding capacities and affinities of liver cytosols and nuclear extracts were measured with established procedures and analyzed by Scatchard plots. Our data revealed significant seasonal variations in both receptor content and the ER's affinity for [3H]E2 in the liver of the turtle. Nuclear ER content remained at a fairly stable level of 70 fmol/g tissue throughout the year, but exhibited two sharp increases in the months of May and October, attaining values of approximately 150 fmol/g tissue. These rises in nuclear ER content coincided temporally with the two peaks of vitellogenin accumulation and estrogen surge in the annual cycle previously reported for the female turtle. Cytosolic ER level in the liver exhibited a much more complex pattern. It fluctuated with a much wider range of 80 to 250 fmol/g tissue. Right after oviposition in July, it declined to its lowest value of 80 +/- 9 fmol/g tissue but gradually increased to 160 +/- 15 fmol/g tissue in September and remained at this medium range for the remaining of the fall season but rose sharply again to the highest value of the year in December, reaching a value of 270 +/- 7 fmol/g tissue. In the following winter months, cytosolic ER level declined until March before it rebounded to a second peak value of 242 +/- 6 fmol/g tissue in May.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Fígado/metabolismo , Receptores de Estrogênio/metabolismo , Estações do Ano , Tartarugas/metabolismo , Animais , FemininoRESUMO
OBJECTIVE: To investigate the effective method to treat cicatricial baldness. METHODS: From 1993 to 1998, 21 cases with multi-region or great-dimensional cicatricial baldness were treated with scalp expanding and hair autografting. Among them, there were 17 males and 4 females, aged from 14 to 49 years old. The operation was divided into two stages, stage one meaned to embed the expander under the scalp and stage two meaned to sow the autogenous hair. RESULTS: All cases, no matter what the position and area, were repaired successfully. The biggest dimension of repaired baldness was 340 cm2, one expander exposed and one failed in expanding after operation and be corrected immediately. The normal hair direction changed in two cases. CONCLUSION: Combined use of scalp expanding and hair autografting is an effective method to treat multi-region or great dimensional cicarticial baldness.
Assuntos
Alopecia/cirurgia , Cicatriz/complicações , Cabelo/transplante , Couro Cabeludo/cirurgia , Adolescente , Adulto , Alopecia/etiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Expansão de Tecido , Transplante AutólogoRESUMO
PrtV is an extracellular metalloprotease of Vibrio parahaemolyticus and regarded as a collagenase. Inductively coupled plasma-optical emission spectrometry analysis indicated that the recombinant PrtV contains 1 mol of zinc per mol of the native enzyme. On the basis of a kinetic study using 2-furanacryloyl-Leu-Gly-Pro-Ala (FALGPA, the specific substrate for bacterial collagenase) as a substrate, it was suggested that metal ions may play a significant role in the binding and catalytic steps of the substrate. PrtV hydrolyzed type I, II, III, and IV collagens; however, it did not hydrolyze type V. In addition, the hydrolysis of native proteins and synthetic substrates revealed that PrtV possesses higher activity toward collagen and collagen-like sequences. The result of the thermal stability study indicated that PrtV was thermostable up to 40 C; at 50 C, stability gradually decreased. In addition, PrtV showed higher storage stability at -20 and 4 C, respectively, than at 25 C. Compared with collagenases from Clostridium histolyticum and Vibrio alginolyticus, PrtV was immunologically different and had no significant effect on the growth of CHO, HeLa, and Vero cells. Taken together, the results of the studies described in this paper advance our knowledge concerning the metal content and biochemical properties of PrtV.
Assuntos
Metais/análise , Colagenase Microbiana/química , Colagenase Microbiana/metabolismo , Vibrio parahaemolyticus/enzimologia , Animais , Catálise , Linhagem Celular , Colágeno/metabolismo , Estabilidade Enzimática , Humanos , Metais/metabolismo , Metais/farmacologia , Colagenase Microbiana/genética , Colagenase Microbiana/toxicidade , Níquel/análise , Oligopeptídeos/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Especificidade por Substrato , Temperatura , Vibrio parahaemolyticus/genética , Zinco/análiseRESUMO
Estrogen receptor (ER) content in the cytosol and nuclear extract of the liver of adult female Chrysemys picta (control females) was measured in February, May, early June, and late June. Both cytosolic (C) and nuclear (N) hepatic ER content rose from low levels (C: 189 +/- 19; N: 52 +/- 23 fmol/g tissue) in February to significantly higher levels in May (C: 437 +/- 15; N: 124 +/- 25 fmol/g tissue) before declining to lower values in early June (C: 298 +/- 19; N: 118 +/- 20 fmol/g tissue) and late June (C: 274 +/- 22; N: 89 +/- 13 fmol/g tissue). This pattern of seasonal variation in hepatic ER content was also observed in gonadectomized females. Levels of hepatic ER content in ovariectomized turtles fluctuated in the same manner as those found in control females. However, elevations in hepatic ER contents were not observed in hypophysectomized females. Removal of the pituitary resulted in a drastic reduction in the May value (316 +/- 49 fmol/g tissue) of cytosolic ER content and a complete blockade in a rise of nuclear ER content in the liver of the turtle. Nuclear hepatic ER content remained at around 50 fmol/g tissue throughout the months of May and June. The two surgeries procedures caused no significant changes in the ER's affinity for estrogen. The present findings demonstrate a direct control of the pituitary over hepatic ER content which signifies important pituitary regulation of the vitellogenic process, at the liver level, during the spring reproductive season.