RESUMO
Parkinson's disease is a common neurodegenerative disorder. Here we present a human induced pluripotent stem cells (iPSCs) derived from peripheral blood mononuclear cells (PBMCs) of a 79-year-old female patient diagnosed with sporadic Parkinson's disease using the sendai virus. Generated iPSCs maintain normal karyotype, exhibit pluripotent stem cell markers, and possess differentiation potential. The iPSCs allows for differentiation into various cell subtypes, providing conditions for the research of the pathogenesis and drug development of Parkinson's disease.
Assuntos
Diferenciação Celular , Células-Tronco Pluripotentes Induzidas , Doença de Parkinson , Humanos , Feminino , Células-Tronco Pluripotentes Induzidas/metabolismo , Idoso , Doença de Parkinson/patologia , Doença de Parkinson/diagnóstico , Leucócitos Mononucleares/citologia , Linhagem Celular , CariótipoRESUMO
Peripheral blood mononuclear cells (PBMCs) of a 38-year-old healthy female were isolated and reprogrammed into the induced pluripotent stem cells (iPSCs). The established iPSC line expressed various pluripotency stem cell markers and potential of differentiating into three germ layers, meanwhile maintained normal karyotype.
Assuntos
Células-Tronco Pluripotentes Induzidas , Feminino , Humanos , Adulto , Células-Tronco Pluripotentes Induzidas/metabolismo , Leucócitos Mononucleares/metabolismo , Camadas Germinativas/metabolismo , Biomarcadores/metabolismo , Diferenciação CelularRESUMO
Peripheral blood mononuclear cells derived from a 35-year-old healthy male were reprogrammed into induced pluripotent stem cells (iPSCs). The iPSCs maintained a normal karyotype, expressed various pluripotency stem cell markers, and showed potential of differentiating into three germ layers. This iPSCs could be differentiated into multiple cell subtypes for drug discovery and investigation of mechanisms.