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The development of novel and effective drug delivery systems aimed at enhancing therapeutic profile and efficacy of therapeutic agents is a critical challenge in modern medicine. This study presents an intelligent drug delivery system based on self-assembled two-dimensional peptide nanosheets (2D PNSs). Leveraging the tunable properties of amino acid structures and sequences, we design a peptide with the sequence of Fmoc-FKKGSHC, which self-assembles into 2D PNSs with uniform structure, high biocompatibility, and excellent degradability. Covalent attachment of thiol-modified doxorubicin (DOX) drugs to 2D PNSs via disulfide bond results in the peptide-drug conjugates (PDCs), which is denoted as PNS-SS-DOX. Subsequently, the PDCs are encapsulated within the injectable, thermosensitive chitosan (CS) hydrogels for drug delivery. The designed drug delivery system demonstrates outstanding pH-responsiveness and sustained drug release capabilities, which are facilitated by the characteristics of the CS hydrogels. Meanwhile, the covalently linked disulfide bond within the PNS-SS-DOX is responsive to intracellular glutathione (GSH) within tumor cells, enabling controlled drug release and significantly inhibiting the cancer cell growth. This responsive peptide-drug conjugate based on a 2D peptide nanoplatform paves the way for the development of smart drug delivery systems and has bright prospects in the future biomedicine field.
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Quitosana , Doxorrubicina , Liberação Controlada de Fármacos , Glutationa , Hidrogéis , Nanoestruturas , Peptídeos , Hidrogéis/química , Doxorrubicina/química , Doxorrubicina/farmacologia , Doxorrubicina/administração & dosagem , Quitosana/química , Glutationa/química , Peptídeos/química , Humanos , Nanoestruturas/química , Sistemas de Liberação de Medicamentos , Portadores de Fármacos/química , Concentração de Íons de HidrogênioRESUMO
Amphiphilic peptides have garnered significant attention due to their highly designable and self-assembling behaviors. Self-assembled peptides hold excellent potential in various fields such as biosensing, environmental monitoring, and drug delivery, owing to their remarkable biological, physical, and chemical properties. While nanomaterials formed by peptide self-assembly have found widespread use in biomedical applications, the development of 2D peptide nanosheets based on the self-assembly of amphiphilic peptides remains challenging in terms of rational design and morphology modulation. In this study, rationally designed amphiphilic peptide molecules are self-assembled into peptide nanosheets (PNS) under specific conditions to encapsulate gold nanoparticles (AuNPs), resulting in the formation of AuNPs/PNS hybrid materials with high photothermal conversion efficiency. The findings demonstrate that 2D PNS enhances the overall photothermal therapy effect of the nanohybrid materials due to their larger hosting area for AuNPs and higher biocompatibility. The well-designed amphiphilic peptides in this study offer insights into the structural design and functional modulation of self-assembled molecules. In addition, the constructed biomimetic-functional 2D inorganic/organic nanohybrid materials hold potential applications in biomedical engineering.
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Functional nanomaterials as nanodrugs based on the self-assembly of inorganics, polymers, and biomolecules have showed wide applications in biomedicine and tissue engineering. Ascribing to the unique biological, chemical, and physical properties of peptide molecules, peptide is used as an excellent precursor material for the synthesis of functional nanodrugs for highly effective cancer therapy. Herein, recent progress on the design, synthesis, functional regulation, and cancer bioimaging and biotherapy of peptide-based nanodrugs is summarized. For this aim, first molecular design and controllable synthesis of peptide nanodrugs with 0D to 3D structures are presented, and then the functional customization strategies for peptide nanodrugs are presented. Then, the applications of peptide-based nanodrugs in bioimaging, chemotherapy, photothermal therapy (PTT), and photodynamic therapy (PDT) are demonstrated and discussed in detail. Furthermore, peptide-based drugs in preclinical, clinical trials, and approved are briefly described. Finally, the challenges and potential solutions are pointed out on addressing the questions of this promising research topic. This comprehensive review can guide the motif design and functional regulation of peptide nanomaterials for facile synthesis of nanodrugs, and further promote their practical applications for diagnostics and therapy of diseases.
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Nanopartículas , Nanoestruturas , Neoplasias , Humanos , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Peptídeos/química , Nanoestruturas/química , Terapia Biológica , Nanopartículas/uso terapêutico , Nanopartículas/químicaRESUMO
Biomimetic synthesis provides potential guidance for the synthesis of bio-nanomaterials by mimicking the structure, properties and functions of natural materials. Behavioral studies of biological surfaces with specific micro/nano structures are performed to explore the interactions of various molecules or organisms with biological surfaces. These explorations provide valuable inspiration for the development of biomimetic surfaces with similar effects. This work reviews some conventional preparation methods and functional modulation strategies for biomimetic interfaces. It aims to elucidate the important role of biomimetic interfaces with antifouling and low-pollution properties that can replace non-environmentally friendly coatings. Thus, biomimetic antifouling interfaces can be better applied in the field of marine antifouling and antimicrobial. In this review, the commonly used fabrication methods for biomimetic interfaces as well as some practical strategies for functional modulation is present in detail. These methods and strategies modify the physical structure and chemical properties of the biomimetic interfaces, thus improving the wettability, adsorption, drag reduction, etc. that they exhibit. In addition, practical applications are presented of various biomimetic interfaces for antifouling and look ahead to potential biomedical applications. By continuously discovering functional surfaces with biomimetic properties and studying their microstructure and macroscopic properties, more biomimetic interfaces will be developed.
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Functional hydrogels show potential application in repairing spinal cord injury (SCI) due to their unique chemical, physical, and biological properties and functions. In this comprehensive review, we present recent advance in the material design, functional regulation, and SCI repair applications of bioactive hydrogels. Different from previously released reviews on hydrogels and three-dimensional scaffolds for the SCI repair, this work focuses on the strategies for material design and biologically functional regulation of hydrogels, specifically aiming to show how these significant efforts can promoting the repairing performance of SCI. We demonstrate various methods and techniques for the fabrication of bioactive hydrogels with the biological components such as DNA, proteins, peptides, biomass polysaccharides, and biopolymers to obtain unique biological properties of hydrogels, including the cell biocompatibility, self-healing, anti-bacterial activity, injectability, bio-adhesion, bio-degradation, and other multi-functions for repairing SCI. The functional regulation of bioactive hydrogels with drugs/growth factors, polymers, nanoparticles, one-dimensional materials, and two-dimensional materials for highly effective treating SCI are introduced and discussed in detail. This work shows new viewpoints and ideas on the design and synthesis of bioactive hydrogels with the state-of-the-art knowledges of materials science and nanotechnology, and will bridge the connection of materials science and biomedicine, and further inspire clinical potential of bioactive hydrogels in biomedical fields.
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Nanopartículas , Traumatismos da Medula Espinal , Regeneração da Medula Espinal , Humanos , Biomassa , HidrogéisRESUMO
Nanomaterial-based phototherapy has become an important research direction for cancer therapy, but it still to face some obstacles, such as the toxic side effects and low target specificity. The biomimetic synthesis of nanomaterials using biomolecules is a potential strategy to improve photothermal therapy (PTT) and photodynamic therapy (PDT) techniques due to their endowed biocompatibility, degradability, low toxicity, and specific targeting. This review presents recent advances in the biomolecule-mimetic synthesis of functional nanomaterials for PTT and PDT of cancers. First, we introduce four biomimetic synthesis methods via some case studies and discuss the advantages of each method. Then, we introduce the synthesis of nanomaterials using some biomolecules such as DNA, RNA, protein, peptide, polydopamine, and others, and discuss in detail how to regulate the structure and functions of the obtained biomimetic nanomaterials. Finally, potential applications of biomimetic nanomaterials for both PTT and PDT of cancers are demonstrated and discussed. We believe that this work is valuable for readers to understand the mechanisms of biomimetic synthesis and nanomaterial-based phototherapy techniques, and will contribute to bridging nanotechnology and biomedicine to realize novel highly effective cancer therapies.
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Nanoestruturas , Neoplasias , Fotoquimioterapia , Humanos , Biomimética , Nanoestruturas/uso terapêutico , Nanoestruturas/química , Neoplasias/tratamento farmacológicoRESUMO
Nitrites widely exist in human life and the natural environment, but excessive contents of nitrites will result in adverse effects on the environment and human health; hence, sensitive and stable nitrite detection systems are needed. In this study, we report the synthesis of Ti3C2 nanosheets functionalized with apoferritin (ApoF)-biomimetic platinum (Pt) nanoparticle (Pt@ApoF/Ti3C2) composite materials, which were formed by using ApoF as a template and protein-inspired biomineralization. The formed nanohybrid exhibits excellent electrochemical sensing performance towards nitrite (NaNO2). Specifically, the Pt@ApoF catalyzes the conversion of nitrites into nitrates, converting the chemical signal into an electrical signal. The prepared Pt@ApoF/Ti3C2-based electrochemical NaNO2 biosensors demonstrate a wide detection range of 0.001-9 mM with a low detection limit of 0.425 µM. Additionally, the biosensors possess high selectivity and sensitivity while maintaining a relatively stable electrochemical sensing performance within 7 days, enabling the monitoring of NaNO2 in complex environments. The successful preparation of the Pt@ApoF/Ti3C2 nanohybrid materials provides a new approach for constructing efficient electrochemical biosensors, offering a simple and rapid method for detecting NaNO2 in complex environments.
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Técnicas Biossensoriais , Técnicas Eletroquímicas , Ferritinas , Nanopartículas Metálicas , Nitritos , Platina , Platina/química , Nitritos/análise , Ferritinas/análise , Nanopartículas Metálicas/química , Titânio/química , Humanos , Materiais Biomiméticos/química , Limite de DetecçãoRESUMO
Peptide molecules have design flexibility, self-assembly ability, high biocompatibility, good biodegradability, and easy functionalization, which promote their applications as versatile biomaterials for tissue engineering and biomedicine. In addition, the functionalization of self-assembled peptide nanomaterials with other additive components enhances their stimuli-responsive functions, promoting function-specific applications that induced by both internal and external stimulations. In this review, we demonstrate recent advance in the peptide molecular design, self-assembly, functional tailoring, and biomedical applications of peptide-based nanomaterials. The strategies on the design and synthesis of single, dual, and multiple stimuli-responsive peptide-based nanomaterials with various dimensions are analyzed, and the functional regulation of peptide nanomaterials with active components such as metal/metal oxide, DNA/RNA, polysaccharides, photosensitizers, 2D materials, and others are discussed. In addition, the designed peptide-based nanomaterials with temperature-, pH-, ion-, light-, enzyme-, and ROS-responsive abilities for drug delivery, bioimaging, cancer therapy, gene therapy, antibacterial, as well as wound healing and dressing applications are presented and discussed. This comprehensive review provides detailed methodologies and advanced techniques on the synthesis of peptide nanomaterials from molecular biology, materials science, and nanotechnology, which will guide and inspire the molecular level design of peptides with specific and multiple functions for function-specific applications.
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Fluorescent bioimaging and photothermal therapy (PTT) techniques have potential significance in cancer diagnosis and treatment and have been widely applied in biomedical and practical clinical trials. This study proposes the molecular design and biofabrication of a two-dimensional (2D) nanoplatform, exhibiting promising prospects for synergistic bioimaging and PTT of tumors. First, biocompatible 2D peptide nanosheets (PNSs) were designed and prepared through peptide self-assembly. These served as a support matrix for assembling polyethylene glycol-modified Ag2S quantum dots (PEG-Ag2SQDs) to form a 2D nanoplatform (PNS/PEG-Ag2SQDs) with unique fluorescent and photothermal properties. The designed 2D nanoplatform not only showed improved photothermal efficacy and an elevated photothermal conversion efficiency of 52.46 %, but also demonstrated significant lethality against tumors in both in vitro and in vivo cases. Additionally, it displays excellent imaging effects in the near-infrared II region, making it suitable for synergistic fluorescent imaging-guided PTT of tumors. This study not only provides a facile approach for devising and synthesizing 2D peptide assemblies but also presents new biomimetic strategies to create functional 2D organic/inorganic nanoplatforms for biomedical applications.
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Nanopartículas , Neoplasias , Pontos Quânticos , Humanos , Fototerapia/métodos , Terapia Fototérmica , Nanopartículas/química , Biomimética , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Neoplasias/patologia , Peptídeos , Linhagem Celular TumoralRESUMO
Due to the good biocompatibility and flexibility of cellulose and the excellent optical, electronic, as well as mechanical properties of carbon nanomaterials (CNMs), cellulose/CNM hybrid materials have been widely synthesized and used in energy storage, sensors, adsorption, biomedicine, and many other fields. In this review, we present recent advances (2016-current) in the design, structural design, functional tailoring and various applications of cellulose/CNM hybrid materials. For this aim, first the interactions between cellulose and CNMs for promoting the formation of cellulose/CNM materials are analyzed, and then the hybridization between cellulose with various CNMs for tailoring the structures and functions of hybrid materials is introduced. Further, abundant applications of cellulose/CNM hybrid materials in various fields are presented and discussed. This comprehensive review will be helpful for readers to understand the functional design and facile synthesis of cellulose-based nanocomposites, and to promote the high-performance utilization and sustainability of biomass materials in the future.
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Carbono/química , Celulose/química , Nanoestruturas/química , HumanosRESUMO
Hydrogen peroxide (H2O2) is one of the intermediates or final products of biological metabolism and participates in many important biological processes of life activities. The detection of H2O2 is of great significance in clinical disease monitoring, environmental protection, and bioanalysis. In this study, Ti3C2-based nanohybrids are prepared by the biological modification and self-assembled peptide nanosheets (PNSs)-based biomimetic synthesis of copper sulfide nanoparticles (CuS NPs), which show potential application in the fabrication of low-cost and high-performance electrochemical H2O2 biosensors. The synthesized CuS-PNSs/Ti3C2 nanohybrids exhibit excellent electrochemical performance towards H2O2, in which CuS NPs can catalyze the decomposition of H2O2 and realize the transformation from a chemical signal to an electrical signal to achieve the purpose of H2O2 detection. The prepared CuS-PNSs/Ti3C2-based electrochemical biosensor platform exhibits a wide detection range (5 µM-15 mM) and a low detection limit (0.226 µM). In addition, it reveals good selectivity and stability and can realize the monitoring of H2O2 in a complex environment. The successful biomimetic synthesis of CuS-PNSs/Ti3C2 hybrid nanomaterials provides a green and friendly strategy for the design and synthesis of functional nanomaterials and also provides a new inspiration for the construction of highly effective electrochemical biosensors for practical detection of H2O2 in various environments.
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Técnicas Biossensoriais , Nanopartículas , Peróxido de Hidrogênio/química , Titânio/química , Biomimética , Nanopartículas/química , Técnicas EletroquímicasRESUMO
The detection of hydrogen peroxide (H2O2) is of great significance in environmental monitoring, enzymatic reactions, and disease diagnosis. Here we present the peptide-mediated biomimetic synthesis of ultrafine platinum nanowires (PtNWs) on graphene oxide (GO) nanosheets for the formation of functional hybrids, which show high potential for the fabrication of colorimetric and electrochemical sensors for the detection of H2O2 with high performance. A multifunctional peptide with the sequence KIIIIKYWYAF was designed to create peptide nanofibers (PNFs) via a controllable self-assembly process, which serves as a bridge between GO nanosheets and PtNWs to form PtNWs-PNFs/GO hybrids. On this basis, a dual-mode sensor platform for both colorimetric and electrochemical sensing of H2O2 was fabricated successfully. The obtained results indicate that the synthesized PtNWs-PNFs/GO hybrids could catalyze the decomposition of H2O2 to generate ËOH radicals with a significant current response, and the ËOH radicals are capable of overoxidizing 3,3',5,5',-tetramethylbenzidine (TMB), producing a blue-colored species with a distinct color change for colorimetric sensing. In addition, due to its high catalytic activity, the fabricated PtNWs-PNFs/GO hybrid-based electrochemical sensor exhibits a wider linear detection range of 0.05 µM-15 mM and a low detection limit of 0.0206 µM, which can be applied to detect H2O2 with high selectivity and sensitivity. Our study provides a green and environmentally friendly synthetic strategy for the preparation of biomimetic materials from PtNWs, and the fabricated colorimetric/electrochemical dual-mode H2O2 sensor platform will have a great impact in bioanalysis, environmental monitoring, and biomedicine.
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Grafite , Nanofios , Grafite/química , Peróxido de Hidrogênio/química , Platina/química , Colorimetria/métodos , BiomiméticaRESUMO
Two-dimensional (2D) materials such as graphene, graphene oxide, transition metal oxide, MXene and others have shown high potential for the design and fabrication of various sensors and biosensors due to their 2D layered structure and unique properties. Compared to traditional fluorescent, electrochemical, and electrical biosensors, colorimetric biosensors exhibit several advantages including naked-eye determination, low cost, quick response, and easy fabrication. In this review, we present recent advances in the design, fabrication, and applications of 2D material-based high-performance colorimetric biosensors. Potential colorimetric sensing mechanisms and optimal material selection as well as sensor fabrication are introduced in brief. In addition, colorimetric biosensors based on different 2D materials such as graphene, transition metal dichalcogenide/oxide, MXenes, metal-organic frameworks, and metal nanoplates for the sensitive detection of DNA, proteins, viruses, small molecules, metallic ions, and others are presented and discussed in detail. This work will be helpful for readers to understand the knowledge of 2D material modification, nanozymes, and the synthesis of hybrid materials; meanwhile, it could be valuable to promote the design, fabrication, and applications of 2D material-based sensors and biosensors in quick bioanalysis and disease diagnostics.