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1.
Rev Saude Publica ; 27(5): 373-7, 1993 Oct.
Artigo em Português | MEDLINE | ID: mdl-8209171

RESUMO

A serological survey of Piry was undertaken of the sera of inhabitants of Catolância-Bahia State, Brazil. Serum results obtained by a vesiculovirus neutralization test of C6/36 cells read by ELISA are compared with those obtained by the classic technique, carried out on newborn mice. The agreement between the results was as high as 98.7% of the 204 sera tested and the neutralization test of C6/36 cells was chosen as the most suitable technique for the sero-survey testing.


Assuntos
Anticorpos Antivirais/sangue , Testes de Neutralização/métodos , Vesiculovirus/imunologia , Animais , Brasil , Células Cultivadas , Ensaio de Imunoadsorção Enzimática/métodos , Humanos , Camundongos , Vesiculovirus/isolamento & purificação
2.
Mem Inst Oswaldo Cruz ; 84(3): 303-7, 1989.
Artigo em Inglês | MEDLINE | ID: mdl-2562487

RESUMO

This paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells are used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice as sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent Mayaro infection. IgG EIA-ICC showed high sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by EIA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection.


Assuntos
Alphavirus/imunologia , Anticorpos Antivirais/análise , Infecções por Togaviridae/diagnóstico , Antígenos Virais , Ensaio de Imunoadsorção Enzimática , Testes de Hemaglutinação , Humanos
3.
Bull Pan Am Health Organ ; 26(3): 201-7, 1992.
Artigo em Inglês | MEDLINE | ID: mdl-1393192

RESUMO

This article describes a classical dengue outbreak caused by dengue serotypes 1 and 4 that occurred from March to July 1990 in the city of Iquitos and surrounding areas of Loreto Department in the Peruvian Amazon. Epidemiologic data indicate that more than 150,000 persons may have been affected in Iquitos alone. Another dengue outbreak occurred in Tarapoto, a city in the neighboring department of San Martín. Laboratory data indicate that the same dengue serotypes were involved in both outbreaks. No cases of dengue hemorrhagic fever/shock syndrome appear to have occurred. Prior to this outbreak, no indigenous dengue cases had been documented in Peru.


Assuntos
Dengue/epidemiologia , Surtos de Doenças , Dengue/sangue , Dengue/microbiologia , Feminino , Humanos , Masculino , Controle de Mosquitos , Peru/epidemiologia , Vigilância da População , Estações do Ano , Sorotipagem
4.
Virology ; 230(2): 244-51, 1997 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-9143280

RESUMO

The recent emergence and spread of dengue hemorrhagic fever in the Americas have been a major source of concern. Efforts to control this disease are dependent on understanding the pathogenicity of dengue viruses and their transmission dynamics. Pathogenicity studies have been hampered by the lack of in vitro or in vivo models of severe dengue disease. Alternatively, molecular epidemiologic studies which associate certain dengue virus genetic types with severe dengue outbreaks may point to strains with increased pathogenicity. The comparison of nucleotide sequences (240 bp) from the E/NS1 gene region of the dengue virus genome has been shown to reflect evolutionary relationships and geographic origins of dengue virus strains. This approach was used to demonstrate an association between the introduction of two distinct genotypes of dengue type 2 virus and the appearance of dengue hemorrhagic fever in the Americas. Phylogenetic analyses suggest that these genotypes originated in Southeast Asia and that they displaced the native, American genotype in at least four countries. Vaccination and other control efforts should therefore be directed at decreasing the transmission of these "virulent" genotypes.


Assuntos
Vírus da Dengue/classificação , Vírus da Dengue/patogenicidade , Dengue/virologia , Sequência de Bases , Brasil , Colômbia , DNA Viral , Vírus da Dengue/genética , Genótipo , Humanos , México , Dados de Sequência Molecular , Filogenia , Venezuela , Proteínas do Envelope Viral/genética , Proteínas não Estruturais Virais/genética
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