From viruses to humans - Exploring the structure-function relationship of the Kesv protein for the future of biomedicine.

Viruses often use ion channel proteins to initialise host infections. Defects in ion channel proteins are also linked to several metabolic disorders in humans. In that instance, modulation of ion channel activities becomes central to development of antiviral therapies and drug design. Kesv, a potassium-selective ion channel protein expressed by Ectocarpus siliculosus virus (EsV), possesses remarkable properties which can help to characterise the molecular basis of the functional processes relevant to virus biology and human physiology. The small structural features of this ion channel could serve as a fundamental primer to study more complex ion channels from humans. Therefore, in spite of their evolutionary distance, the potential link between viral and human ion channel proteins could provide opportunities for therapeutic and biotechnological applications.

Spatio-temporal characteristics and multi-scenario simulation analysis of ecosystem service value in coastal wetland: A case study of the coastal zone of Hainan Island, China.

Coastal wetland ecosystems harbor rich biodiversity and possess significant ecosystem service value (ESV). Therefore, it offers a range of crucial ecosystem services (ES) for human well-being and socio-economic development. Taking the Hainan Island coastal zone (HICZ) as a case study, the spatio-temporal characteristics of land use and land cover change (LULCC), and its associated ESV in wetland landscapes were analyzed over three time points (2000, 2010 and 2020). We explored the spatio-temporal evolution trajectory of ESV on the basis of geo-information tupu. Then, future land use simulation (FLUS) was employed to predict wetland patterns and ESV under three different scenarios: business as usual (BAU), ecological conservation first (ECF), and economic development first (EDF). The results showed that over the past two decades, a significant proportion (exceeding 80%) of the overall wetland region was comprised of offshore and coastal wetlands (OCW) as well as constructed wetlands (CW); these formed the matrix of the landscape. The area of building land (BL) continued to exhibit a consistent upward trend. Expanding by 2.18 times, it represented the most significant increase in the rate of dynamic changes in BL. The main ES in the HICZ corresponded to the regulation services (53.57%) and the support services (27.58%). The ESV of wetland losses accounted for 45.17% (43.08 × 108 yuan) of the total loss. The spatial differentiation of ESV in the HICZ was larger in the southwest and the northeast regions, while it was comparatively lower in the north. The transformation in the area of early and late change types accounted for 236.46 km2 and 356.69 km2, respectively. The scenario ECF was achieved with an optimal development of ESV (1807.72 × 108 yuan), which was coordinated with the high-level of development of regional ES functions and the economy. These findings provide valuable information for the sustainable development as well as the protection of ecology and environment of the coastal zone under the background of the construction of Hainan pilot free trade zone in the future.

Impact of changing urban form and production-living-ecological space on changing ecosystem services of a smart city of Eastern India, Durgapur.

Worldwide land use land cover (LULC) transformation become a serious issue in the last few decades due to its immense importance in environmental and human well-being perspectives. Expansion of urban areas at the expense of natural land covers and changing urban form is mainly responsible for changing environmental conditions. This study focused on identifying the impacts of LULC change on environmental conditions through the assessment of changing ecosystem services (ESs) of the Durgapur Municipal Corporation (DMC) from 1990 to 2020. Changing ESs are assessed based on changing urban forms and production-living-ecological space (PLES) components. Results found that the compactness of urban areas is increasing along with the outward expansion. The core urban area of DMC has risen from 8.11% to 30.11% during 1990-2020. Similarly, living space increased from 15.57% to 42.60%, production space decreased from 53.06% to 25.59%, and ecological space fluctuated from 1990 to 2020. This transformation of PLES components negatively affects DMC's environmental condition, affecting the achievement of Sustainable Development Goals (SDGs). These significant results may be utilized to understand changing environmental conditions and priority issues for DMC's future sustainable urban development.

Dynamic simulation and projection of ESV changes in arid regions caused by urban growth under climate change scenarios.

Spatial simulation and projection of ecosystem services value (ESV) changes caused by urban growth are important for sustainable development in arid regions. We developed a new model of cellular automata based grasshopper optimization algorithm (named GOA-CA) for simulating urban growth patterns and assessing the impacts of urban growth on ESV changes under climate change scenarios. The results show that GOA-CA yielded overall accuracy exceeding 98%, and FOM for 2010 and 2020 were 43.2% and 38.1%, respectively, indicating the effectiveness of the model. The prairie lost the highest economic ESVs (192 million USD) and the coniferous yielded the largest economic ESV increase (292 million USD) during 2000-2020. Using climate change scenarios as urban future land use demands, we projected three scenarios of the urban growth of Urumqi for 2050 and their impacts on ESV. Our model can be easily applied to simulating urban development, analyzing its impact on ESV and projecting future scenarios in global arid regions.

Genetic background of anti-Xga producers in Japanese blood donors.

BACKGROUND AND OBJECTIVES: The Xg blood group is composed of two antigens, Xga (XG1) and CD99 (XG2 and MIC2). The XG and CD99 are homologous genes located on pseudoautosomal region 1 of the X and Y chromosomes. The expressions of Xga and CD99 are co-regulated by a single nucleotide polymorphism (rs311103) in the GATA-1 binding region. Another mechanism of the Xg(a-) phenotype is the genomic deletion of approximately 114 kb, including the XG gene. Anti-Xga seems to be naturally occurring by detection in males who have never been transfused. MATERIALS AND METHODS: In this study, we identified 23 anti-Xga producers among 580,115 donors (0.004%). Additional 12 anti-Xga producers were also identified from a separate cohort. RESULTS: All 35 anti-Xga producers were male. Genomic DNA was obtained from 34 of 35 producers, and all 34 producers were confirmed to carry the XG-gene-deficient allele (XGdel). The breakpoints of all 34 producers were identical. The XGdel was also identified in 12 non-producers of anti-Xga among 860 donors who have no antibodies against RBCs, and the breakpoints were also identical with the anti-Xga producers. CONCLUSION: Our results will serve as the basis for a more complete understanding of Xg blood group polymorphisms.

Three-dimensional right ventriculo-arterial coupling as an independent determinant of severe heart failure symptoms in patients with dilated cardiomyopathy.

BACKGROUND: Right ventricular-pulmonary artery coupling (RVPAC) is a predictor of outcome in pulmonary hypertension. However, the role of this parameter in dilated cardiomyopathy (DCM) remains to be established. The aim of this study was to assess the contribution of RVPAC to the occurrence of severe heart failure (HF) symptoms in patients with DCM using three-dimensional (3D) echocardiography. METHODS: We prospectively screened 139 outpatients with DCM, 105 of whom were enrolled and underwent 3D echocardiographic assessment. RVPAC was estimated non-invasively as the 3D right ventricular stroke volume (SV) to end-systolic volume (ESV) ratio. Severe HF symptoms were defined by New York Heart Association (NYHA) class III or IV. We evaluated differences in RVPAC across NYHA classes and the ability of RVPAC to predict severe symptoms. RESULTS: Mean left ventricular (LV) ejection fraction was 28±7%. Mean RVPAC was 0.77±0.30 and it was significantly more impaired with increasing symptom severity (p = 0.001). RVPAC was the only independent determinant of severe HF symptoms, after adjusting for age, diuretic use, LV systolic function, LV diastolic function, and pulmonary artery systolic pressure (OR 0.035 [95% CI, 0.004-0.312], p = 0.003). By receiver-operating characteristic analysis, the RVPAC cut-off value for predicting severely symptomatic status was 0.54 (area under the curve = 0.712, p < 0.001). CONCLUSION: 3D echocardiographic SV/ESV ratio is an independent correlate of severe HF symptoms in patients with DCM. 3D RVPAC might prove to be a useful risk stratification tool for these patients, should it be further validated in larger studies.

Unraveling the dynamics, heterogeneity, determinants of eco-efficiency in Beijing-Tianjin-Hebei urban agglomeration, China.

Eco-efficiency has been considered a valuable gauge for evaluating how efficient economic activities are in regard to resource inputs and eco-environmental pressures. Even though Ecosystem services (ESs) are inseparable from sustainable eco-environment, a paucity of literature has considered ESs in eco-efficiency research lines. Therefore, this study aims to construct a novel eco-efficiency evaluation framework by integrating ESs as natural capital input and measure it utilizing the Epsilon-based measure model for the county-level cities in Beijing-Tianjin-Hebei urban agglomeration (BTHUA) during the period 2005-2015. The spatial econometric technique is further performed to acquire quantitative evidence about whether ESs and other determinants impact eco-efficiency. The results revealed that eco-efficiency increased continuously in the whole BTHUA and BTHUA's optimized development functional areas, whereas eco-efficiency of BTHUA's sub-regions showed a significant temporal diversity. The average eco-efficiency values of cities in key development functional areas and restricted development functional areas showed the V-shaped trend (declining before 2010 and then rising). Interestingly, this study found that ESV economic loss may result in eco-efficiency decline for cities located in key development functional areas. From the spatial heterogeneity perspective, the city with high EE is mainly located in eastern BTHUA, whereas cities in the northern plateau areas, southwestern, and western BTHUA have relatively low EE. Furthermore, there existed a significant spatial autocorrelation and a spatial agglomeration heterogeneity, which suggests that the low-low correlation regions gradually being the most dominant spatial pattern. The results of spatial econometric model verified that water yield has the strongest positive effect on EE while soil erosion will lead to declining EE. This paper potentially provides new insights for future policy design of urban agglomeration sustainable deployment.

The impact of frame numbers on cardiac ECG-gated SPECT images with interpolated extra frames using echocardiography.

Background: Cardiac echocardiography and cardiac ECG-gated single-photon emission computed tomography (SPECT) are the most common modalities for left ventricle (LV) volumes and function assessment. The temporal resolution of SPECT images is limited and an ECG provides better temporal resolution. This study investigates the impact of frame numbers on images in terms of qualitative and quantitative assessments. Methods: In this study, 5 patients underwent echocardiography and cardiac ECG-gated SPECT imaging, and 5 standard views of the LV were recorded to determine LV walls boundaries and volumes. Also, 2 original images with 8 frames and 16 frames per cardiac cycle were recorded simultaneously in a single gantry orbit. Using the data extracted from the LV model, 8 extra new frames were created with interpolation between existing frames of the original 8-frame image. Three series of images (8 and 16 original and 16 interpolated) were reconstructed separately. LV volumes and ejection fraction (EF) were calculated using Quantitative Gated SPECT (QGS) software. Results: Compared to the original 8-frame gating, original 16-frame gated images resulted in larger end-diastole volume (EDV) (mean ± SD: 68.6 ± 27.11 mL vs 66.2±25.41 mL, p<0.001), smaller end-systole volume (ESV) (mean ± SD: 24.6±8.7 mL vs 26±7.3 mL, p<0.001), and higher EF (64% vs 60.2%, p<0.001). The results for the interpolated series were also different from the original images (closer to the original 16-frame series rather than 8-frame). Conclusion: Changing the frame number from 8 to 16 in cardiac ECG-gated SPECT images caused a significant change in LV volumes and EF. Frame interpolation with sophisticated algorithms can be used to improve the temporal resolution of SPECT images.

Spatial-temporal variation and correlation analysis of ecosystem service values and ecological risks in winter city Shenyang, China.

Ecosystems in winter cities are complex and fragile, experiencing significant changes due to climate variations and human construction activities. Previous studies on the assessment of overall ecosystem service value (ESV) and ecological risk index (ERI) in winter cities are scarce. In this study, we constructed ESV and ERI measurement models using land use data in 2000, 2010, and 2020 using the improved value per unit area factor method and the landscape pattern index method, respectively, to reveal their spatial and temporal change characteristics. Geographic detectors were used to explore the driving roles of natural and artificial factors on the changes of ESV and ERI. The combination in ESV and ERI can then provide a more quantitative and accurate basis for policy decisions, identify priority areas for urban ecological restoration, and reduce the risk to ecosystems. The results of the study show that the total ESV of Shenyang city decreased from 273.97 × 108 CNY to 270.38 × 108 CNY during 2000-2020. Although the decrease is not large, the ESV changes structurally with the advancement of urbanization. During the 20 years, the construction land with the lowest ecological service function continues to expand, increasing by 354 km2, the grassland decreased by 215.9 km2, and the arable land decreased by 196.6 km2. The ecological service function of the water area is the strongest, with an increase of 51.3 km2 in the water area, ensuring that there is no significant decline in ESV. The size of the ERI is Very high, High, and Medium value zones remained relatively stable, while the size of the Very Low-value zone decreased by 12.78% and the size of the Low-value zone increased by 13.21%. The interaction factors that contributed most to the changes in ESV and ERI were annual evapotranspiration (EVP)/ Normalized Difference Vegetation Index (NDVI) and Annual sunshine hours (SSD)/ Digital Elevation Model (DEM) , respectively. There was a spatial correlation between ESV and ERI. The areas with the highest ESV supply capacity and at the same time facing severe ecological risks to the landscape pattern are distributed in the northeastern hilly lands. This area should be prioritized to develop planning and control measures to prevent further erosion of forest lands and grasslands and reduce ecological risks. These results provide a theoretical basis for ensuring ecological security and sustainable development in winter cities.

[Spatiotemporal Response of Ecosystem Service Value to Land Use Change in the Lanzhou-Xining Urban Agglomeration over the Past 20 Years].

With rapid urbanization and human activities exacerbating threats to the degradation of various ecosystem services in modern urban agglomerations, the exploration of the state of ecological security at the scale of urban agglomerations is of great significance. This study considered the Lanzhou-Xining Urban Agglomeration as the research area, based on the land use data in 2000, 2005, 2010, 2015, and 2020. At the same time, the landscape ecological risk index was introduced. The land use change characteristics of the Lanzhou-Xining Urban Agglomeration were analyzed by using the land use transfer matrix, the value per unit area equivalent factor method, and the bivariate spatial autocorrelation analysis method to elucidate the impacts of the changes in the ecological risk index induced by the land use transition on the value of ecosystem services. This study analyzed the land use change characteristics of the Lanzhou-Xining Urban Agglomeration and elucidated the impacts of changes in the ecological risk index on the value of ecosystem services caused by land use transformation. The results showed that:① During the period from 2000 to 2020, the land use types of the Lanzhou-Xining Urban Agglomeration were mainly dominated by grassland, cropland, and forest land. The construction land area had expanded significantly mainly from cropland and grassland, and the six land use types had strong cross-transformation. The total area of land use change was 6 646.05 km2. ② In terms of spatial changes, the spatial pattern of ecosystem service value in the Lanzhou-Xining Urban Agglomeration had not undergone obvious transformation. However, the regional variability was significant, generally showing the distribution characteristics of high in the northwest and low in the southeast. ③From the perspective of temporal change, the value of ecosystem services in the Lanzhou-Xining Urban Agglomeration showed an upward trend, with the total flow of value increasing from 186.459 billion yuan to 192.156 billion yuan, with a total value-added of 5.697 billion yuan. ④ There was a rising trend in the overall ecological risk index of the Lanzhou-Xining Urban Agglomeration over the past 20 years. Low ecological risk areas and lower ecological risk areas dominated the ecological risk areas. There was a significant positive correlation between the value of ecosystem services and the ecological risk index. This study aimed to reveal the understanding of the impacts of land-use practices on ecosystem service values and ecological risks, to provide important references for regional ecological risk management and land-use policy formulation, and thus to promote the high-quality development of the ecological environment in the Yellow River Basin.

Impact of land use land cover changes on ecosystem service values in the Dire and Legedadi watersheds, central highlands of Ethiopia: Implication for landscape management decision making.

Land use land cover change in a landscape is the main driver of degradation in ecosystem goods and services. This study was aimed at analysing the dynamics of the LULC change in the catchments of the water supply reservoirs as well as the impact on the Ecosystem Service Values (ESVs) between 1985 and 2022. The benefit transfer method was used to evaluate ecosystem service value (ESV) changes in response to LULC. The watersheds experienced substantial LULC changes. As a result, the natural vegetation, grasslands, and eucalyptus plantations declined dramatically, while settlements and cultivated lands considerably increased. The global and local ESV estimates show a dramatic decline in ESVs between 1985 and 2022. According to global and local ESV estimates, total ESV in the Legedadi watershed has decreased from approximately US$ 65.8 million in 1985 to approximately US$ 11.9 million in 2022 and from approximately US$ 42.7 million in 1985 to approximately US$ 9.66 million in 2022. According to global and local ESV estimates, total ESV in the Dire watershed decreased from approximately US$ 437 thousand in 1985 to approximately US$ 59 thousand in 2022 and from approximately US$ 225 thousand in 1985 to approximately US$ 36 thousand in 2022. The overall decline in ESV demonstrates that the natural environment is deteriorating as a result of replacement of the natural land cover by other economic land uses. Hence, it is highly recommended that implementing sustainable watershed management practices to halt the dramatic loss of natural ecosystems must be a high priority.

Integrating Landscape Ecological Risks and Ecosystem Service Values into the Ecological Security Pattern Identification of Wuhan Urban Agglomeration.

Urban agglomerations are the main form of China's future promotion of new urbanization development. Nevertheless, their accelerated expansion and development are increasingly threatening the security of regional ecosystems. The identification and optimization of ecological safety patterns (ESPs) is the fundamental spatial way to guarantee the ecological safety of urban circles and realize the sustainable development of the socio-economic and ecological environment. Nevertheless, from the perspective of urban green, low-carbon, and ecological restoration, regional safety evaluation still lacks a complete framework integrating ecological elements and social and natural indicators. Moreover, the evaluation method of ESPs also has a lack of judgment on the long-term change dynamics of regional landscape ecological risks and ecosystem service values. Thus, we proposed a new regional ecological security evaluation system based on ecosystem service value (ESV) and landscape ecological risk (LER), using the Wuhan urban agglomeration (WUA) as the research object. This study analyzed LER and ESV's spatial and temporal changes over nearly 40 years from 1980 to 2020. LER and LSV were used as ecological elements combined with natural and human-social elements to jointly model the resistance surface of the landscape pattern. Applying the minimum cumulative resistance model (MCR), we identified green ecological corridors, constructed the ESPs of WUA, and proposed optimization measures. Our results show that: (1) The proportion of higher- and high-ecological-risk areas in WUA has decreased from 19.30% to 13.51% over the past 40 years. Over time, a "low-high-low" hierarchical distribution characteristic centered on Wuhan city was gradually formed in the east, south, and north; the total value of ecosystem services increased from CNY1110.998 billion to CNY1160.698 billion. The ESV was higher in the northeastern, southern, and central parts of the area. (2) This study selected 30 ecological source areas with a total area of about 14,374 km2 and constructed and identified 24 ecological corridors and 42 ecological nodes, forming a multi-level ecological network optimization pattern with intertwined points, lines, and surfaces, increasing the connectivity of the ecological network and improving the ecological security level of the study area to a large extent, which is of great significance to promote the ecological priority and green-rise strategy of WUA and the high-quality development path of the green ecological shelter.

Prediction of angiographic coronary disease and mortality with a cadmium-zinc-telluride camera: a comparison of upright and supine ejection fractions and left ventricular volumes.

Introduction: Perfusion imaging strongly predicts coronary artery disease (CAD), whereas cardiac volumes and left ventricular ejection fraction (LVEF) strongly predict mortality. Compared to conventional Anger single-photon emission computed tomography (SPECT) cameras, cadmium-zinc-telluride (CZT) cameras provide higher resolution, resulting in different left ventricular volumes. The cadmium-zinc-telluride D-SPECT camera is commonly used to image in the upright position, which introduces changes in left ventricular loading conditions and potentially alters left ventricular volumes. However, little or no data exist on the predictive value of left ventricular volumes and ejection fraction when acquired in the upright position. We investigated models for the prediction of CAD and mortality, comparing upright and supine imaging. Methods: A retrospective study of patients with upright/supine stress and rest imaging and coronary angiography within 3 months was performed. Univariate and multivariable analyses were performed to predict abnormal angiograms and all-cause mortality. Results: Of the 392 patients, 210 (53.6%) had significant angiographic CAD; 78 (19.9%) patients died over 75 months. The best multivariable model for CAD included the supine summed stress score and supine stress LVEF, with an area under the receiver operating characteristic of 0.862, a sensitivity of 76.7%, and a specificity of 82.4%, but this model was not statistically superior to the best upright model. The best multivariable models for mortality included age, diabetes, history of cardiovascular disease, and end-systolic volume, with the upright and supine models being equivalent. Discussion: Angiographic CAD was best predicted by the supine summed stress score and LVEF but was not statistically superior to the next-best upright model. Mortality was best predicted by end-systolic volume in combination with age, diabetes status, and cardiovascular disease status, with equivalent results from the upright and supine images.

Evaluation and prediction of land use change impacts on ecosystem service values in Nanjing City from 1995 to 2030.

Land use changes have a considerable impact on ecosystem services (ESs). In recent years, land use changes caused by urban expansion in Nanjing City have been obvious and are expected to further change in the future. Therefore, it is urgent to quantitatively assess ecosystem service value (ESV) changes caused by previous land use changes and future potential changes in Nanjing. In this study, land use data products based on remote sensing images, Dyna-CLUE model, and the ESV equivalent coefficient method were applied to assess the impact of land use changes on ESVs in Nanjing City over the past 23 years (1995-2018), and to forecast the changes of ESV in 2030. The results indicated that the total ESVs of Nanjing City displayed a trend of first increasing and then declining in 1995-2018. From the land use classification, the water area had the largest ESV in Nanjing, followed by arable land. Additionally, the regulating service value was the highest among the four primary ESs in Nanjing from 1995 to 2030, with the highest value of 13.73 billion yuan in 2015. Among the three forecast scenarios, the ecological protection scenario had the highest total ESV and was followed by the urban expansion and business as usual scenarios. These findings may assist for the scientific decision-making of sustainable land use and ecosystem management in Nanjing City.

Ecological consequences of urban blue space transformation.

This study presents the ecological consequences of the blue space conversion and its qualitative degradation in the English Bazar Municipality (EBM) and its surrounding area. The primary blue spaces of the area, the marshy wetland called Chatra and Mohananda river, are the most affected due to urban activities like built-up expansion and sewage and wastewater discharge. Built-up development encroached more than 300 m within wetland territory and caused a 0.57 km2 conversion of wetland area. It is also evident within the bed of the Mohananda river. Agriculture also caused the conversion of the blue space. As a result, the wetland's ecosystem service value (ESV) was reduced by 12.7%, along with a reduction of cultural services by 27.86%. The massive pouring of sewage and wastewater caused hyper-eutrophication in almost the entire wetland area. The trophic state index (TSI) value increased significantly in the last 10 years, causing high growth and areal expansion of water hyacinth. The expanding settlements and agricultural land that captured the river channel face inundation vulnerability during peak discharge. Extreme danger level discharge causes floods in the extensive municipality area. The areal encroachment, water extraction, sewage and wastewater discharge, and water quality deterioration caused severe hydro-ecological degradation of the river. Since blue space is critically essential for urban environmental health, these ecological consequences can cause a crisis for urban wellbeing. Therefore, the anthropogenic adversities towards the urban blue space must be restricted, and the blue space's ecological sustenance must be paid enough attention.

Reshaping the Ventricle From Within: MIRTH (Myocardial Intramural Remodeling by Transvenous Tether) Ventriculoplasty in Swine.

MIRTH (Myocardial Intramural Remodeling by Transvenous Tether) is a transcatheter ventricular remodeling procedure. A transvenous tension element is placed within the walls of the beating left ventricle and shortened to narrow chamber dimensions. MIRTH uses 2 new techniques: controlled intramyocardial guidewire navigation and EDEN (Electrocardiographic Radial Depth Navigation). MIRTH caused a sustained reduction in chamber dimensions in healthy swine. Midventricular implants approximated papillary muscles. MIRTH shortening improved myocardial contractility in cardiomyopathy in a dose-dependent manner up to a threshold beyond which additional shortening reduced performance. MIRTH may help treat dilated cardiomyopathy. Clinical investigation is warranted.

Improved mid-term stability of MR reduction with an increased number of clips after percutaneous mitral valve repair in functional MR.

Background: Percutaneous mitral valve repair (PMVR) has evolved to be a standard procedure in suitable patients with mitral regurgitation (MR) not accessible for open surgery. Here, we analyzed the influence of the number and positioning of the clips implanted during the procedure on MR reduction analyzing also sub-collectives of functional and degenerative MR (DMR). Results: We included 410 patients with severe MR undergoing PMVR using the MitraClip® System. MR and reduction of MR were analyzed by TEE at the beginning and at the end of the PMVR procedure. To specify the clip localization, we sub-divided segment 2 into 3 sub-segments using the segmental classification of the mitral valve. Results: We found an enhanced reduction of MR predominantly in DMR patients who received more than one clip. Implantation of only one clip led to a higher MR reduction in patients with functional MR (FMR) in comparison to patients with DMR. No significant differences concerning pressure gradients could be observed in degenerative MR patients regardless of the number of clips implanted. A deterioration of half a grade of the achieved MR reduction was observed 6 months post-PMVR independent of the number of implanted clips with a better stability in FMR patients, who got 3 clips compared to patients with only one clip. Conclusions: In patients with FMR, after 6 months the reduction of MR was more stable with an increased number of implanted clips, which suggests that this specific patient collective may benefit from a higher number of clips.

Research on Expansion Characteristics of Aquaculture Ponds and Variations in Ecosystem Service Value from the Perspective of Protecting Cultivated Lands: A Case Study of Liyang City, China.

In the context of global food insecurity, a large amount of cultivated land in China has been occupied by aquaculture ponds, leading to a series of variations in the ecological environment. The Chinese government pays close attention to the problem. In order to achieve sustainable development and ensure the safety of China's cultivated land, the paper uses Liyang City as an example to discuss the spatial characteristics of the expansion of aquaculture ponds through occupying cultivated lands and analyzes the variations in ecosystem service value and cultivated land function. The conclusions are as follows: (1) 2073.24 hectares of cultivated lands were occupied for expanding aquaculture ponds in Liyang from 2009 to 2019, and there was a small number of new aquaculture ponds in the ecological protection area, which shows that the aquaculture ponds in Liyang City are at the stage of disorderly expansion; (2) the total value of ecosystem services increased by 1.43%; supply and support services values decreased, but the increase in regulation and cultural services values was sufficient to more than compensate for the mentioned losses; and (3) the expansion of aquaculture ponds leads to a decrease in the carbon storage of cultivated land, which in turn has negative impacts such as an increase in atmospheric carbon concentration.

The Effects of the Ecological Conservation Redline in China: A Case Study in Anji County.

The Ecological Conservation Redline (ECR) of China plays an important role in avoiding ecological space occupancy and maintaining regional ecological security. Anji County in Zhejiang Province is one of the first regions to implement the ECR in China. This paper takes Anji County as an example to analyze the effects of ECR. To do this, we first set up two scenarios with the CLUE-S model: a normal land-use development scenario (NLDS) and an ECR implementation scenario (ECRS); then we compare the land use of 2010 and 2015 under NLDS and ECRS. Land use, ecosystem services value (ESV), landscape metrics, and ecological product outputs were compared between the entire county and the ECR areas. The results revealed the following: (1) From 2000 to 2015, the ecological land in Anji County decreased by 4.03%, while it decreased by 1.17% in the ECR areas. (2) In the ECR areas, there was less arable land and construction land of the ECRS than in the NLDS, which indicates the ECR impeded the expansion of construction land and arable land in the ECR areas. (3) The ECR areas account for 39% of Anji County but contribute more than 80% to the ESV of the whole county. During 2000-2015, the ESV of the entire county decreased while the ESV of the ECR areas increased. (4) From 2000 to 2015, whereas landscape fragmentation of the entire county increased, that of ECR areas decreased. (5) Since the ECR's implementation, Anji County has vigorously developed the bamboo industry, ecological agriculture, the tourism industry, and achieved rapid economic development via industrial restructuring and transformation. On the whole, the ECR has neither adversely affected land development nor economic development but instead has promoted the optimization of the land's spatial development pattern.

Quantifying the Relationship between Land Use Intensity and Ecosystem Services' Value in the Hanjiang River Basin: A Case Study of the Hubei Section.

An increased land use intensity due to rapid urbanization and socio-economic development would alter the structure and function of regional ecosystems and cause prominent environmental problems. Revealing the impact of land use intensity on ecosystem services (ES) would provide guidance for more informed decision making to promote the sustainable development of human and natural systems. In this study, we selected the Hanjiang River Basin (HRB) in Hubei Province (China) as our study area, explored the correlation between land use intensity and ecosystem Services' Value (ESV), and investigated impacts of natural and socio-economic factors on ESV variations based on the Geographical Detector Model (GDM) and Geographically Weighted Regression (GWR). The results show that (1) from 2000 to 2020, land use intensity in HRB generally showed an upward trend, with a high spatial agglomeration in the southeast and low in the northwest; (2) the total ESV increased from 295.56 billion CNY in 2000 to 296.93 billion CNY in 2010, and then decreased to 295.63 CNY in 2020, exhibiting an inverted U-shaped trend, with regulation services contributing the most to ESV; (3) land use intensity and ESV had a strong negative spatial correlation, with LH (low land use intensity vs. high ESV) aggregations mainly distributed in the northwest, whereas HL (high land use intensity vs. low ESV) aggregations were located in the southeast; (4) natural factors, including annual mean temperature, the percentage of forest land, and slope were positively associated with ESV, while socio-economic factors, including GDP and population density, were negatively associated with ESV. To achieve the coordinated development of the socio-economy and the environment, ES should be incorporated into spatial planning and socio-economic development policies.