Elucidating biofouling development and succession in membrane distillation using treated effluent.

Biofouling in membrane distillation (MD) has several repercussions, including reduced efficiency of the MD process and limiting membrane life. Additionally, the evaluation of MD biofouling using treated effluents from wastewater treatment plants remains an unexplored area. Thus, biofouling formation and development in a long term MD process (15 days) using treated effluent from a wastewater treatment plant was explored in this study. The results revealed that flux decline occurred in four phases: i) initial decline (0-1 d), ii) gradual decline (1-5 d), iii) progressive decline (5-10 d), and iv) rapid decline (10-15 d). Liquid Chromatography-Organic Carbon Detection (LC-OCD) analysis demonstrated that the treated effluent contained humic-like substances, which deposited on the membrane surface in phase 1. Whereas biopolymers development on the membrane surface in phase 2 and 3 was linked to biofouling. Microbial community analysis revealed that the initial colonisers were predominantly thermophilic bacteria, which were different from the microbial community of the treated effluent. The biofilm-forming bacteria included Schlegelella, Meiothermus, and Vulcaniibacterium. These microorganisms proliferate and release excessive extracellular polymeric substances (EPS), leading to the development of mature biofilm on membrane surface. This helped in the deposition of organics and inorganics from the bulk feed, which led to microbial community succession in phase 4 with the emergence of the Kallotenue genus. The results suggested that organic substances and microbial communities on membrane surface at different stages in a long-term MD process had a significant influence on MD performance for high-quality wastewater reuse.

Flow path monitoring by discontinuous time-lapse ERT: An application to survey relationships between secondary effluent infiltration and roots distribution.

The infiltration of secondary treated effluent (STE) into the soil downstream of wastewater treatment plants is becoming increasingly common in a climate change context. In STE infiltration, STE is discharged onto the soil over a large surface allowing for a gradual infiltration of the water. This paper investigates a novel time-lapse electrical resistivity tomography strategy to evaluate the impact of STE infiltration on the water pathways of two planted loamy-soil trenches located in a Fluvisol region in southwestern France. The system has been monitored for 3 years using discontinuous monitoring of electrical resistivity tomography during four saline tracer tests. Results show that: 1) the new methodology has successfully highlighted the evolution of water pathways in the soil over time; 2) such evolution is in agreement with reeds root distribution in the trenches which seems to be affected by water quality i.e. sludge losses and TSS, for this study case. Indeed, for the infiltration trench receiving STE with lower pollution levels (2.2 mg TSS. L-1, 26 mg COD. L-1), the infiltration capacity is maintained over the years (4-6 mm h-1) and reed roots developed deeper in the soil. A sludge deposit present at the bottom of the second infiltration trench receiving higher pollution levels (7.2 mg TSS. L-1, 45 mg COD. L-1, plus episodic sludge release) could lead roots to develop close to the surface affecting the infiltration capacity which did not evolve over time. This work highlights the importance of long-term flow pathway monitoring in understanding the hydraulic behavior of infiltration surfaces submitted to STE.

Combination of physico-chemical analysis, Allium cepa test system and Oreochromis niloticus erythrocyte based comet assay/nuclear abnormalities tests for cyto-genotoxicity assessments of treated effluents discharged from textile industries.

Bioassays for cyto-genotoxicity assessments are generally not required in current textile industry effluent discharge management regulations. The present study applied in vivo plant and fish based toxicity tests viz. Allium cepa test system and Oreochromis niloticus erythrocyte based comet assay and nuclear abnormalities tests in combination with physico-chemical analysis for assessing potential cytotoxic/genotoxic impacts of treated textile industry effluents reaching a major river (Kelani River) in Sri Lanka. Of the treated effluents tested from two textile industries, color in the Textile industry 1 effluents occasionally and color, biochemical oxygen demand and chemical oxygen demand in the Textile industry 2 effluents frequently exceeded the specified Sri Lankan tolerance limits for discharge of industrial effluents into inland surface waters. Exposure of A. cepa bulbs to 100% and 12.5% treated effluents from both industries resulted in statistically significant root growth retardation, mito-depression, and induction of chromosomal abnormalities in root meristematic cells in comparison to the dilution water in all cases demonstrating cyto-genotoxicity associated with the treated effluents. Exposure of O. niloticus to the 100% and 12.5% effluents, resulted in erythrocytic genetic damage as shown by elevated total comet scores and induction of nuclear abnormalities confirming the genotoxicity of the treated effluents even with 1:8 dilution. The results provide strong scientific evidence for the crucial necessity of incorporating cyto-genotoxicity impact assessment tools in textile industry effluent management regulations considering human health and ecological health of the receiving water course under chronic exposure.

Impact of untreated and microbially treated equalization tank effluent of textile industry on freshwater fish Channa punctata using haematological, biochemical, histopathological and ultrastructural analysis.

The unregulated expulsion of untreated or partially treated industrial effluents poses serious threat to the aquatic ecosystem. Therefore, in the present study fish Channa punctata were exposed to untreated and microbially treated equalization tank effluent of textile industry and toxicity studies were carried out for 45 days. The study was planned to analyze the toxicity proffered by textile effluents through haematological, biochemical, histopathological and ultrastructural analysis in blood, liver and gill tissues of fish. While comparing untreated and microbially treated effluent exposed groups haematological parameters were significantly (P ≤ 0.05) less in the untreated effluent exposed group whereas White blood cell count was highly escalated. However, in the microbially treated groups, the alterations were less severe. Increased malondialdehyde content indicating oxidative stress, reduced Catalase (CAT) and Superoxide dismutase (SOD) activity showing a weakened antioxidant defence system and increased glutathione activity was also perceived in untreated effluent exposed groups in comparison to microbially treated groups. Histopathological alterations in gill (telangiectasia, lamellae fusion, breakage, vacuolization and bending of lamellae) and liver (sinusoid dilations, fusion, necrosis and congestion) were more pronounced and severe in the untreated effluent exposed group as compared to microbially treated group. The results observed in histopathology were further reaffirmed by scanning electron microscopy. The study clearly highlights less alterations and deformities in microbially treated effluent groups in comparison to untreated effluent groups. These findings, therefore, necessitate the search for more effective microbial inocula for the better treatment of effluents in order to protect the aquatic life as well as human beings. Highlights: Channa punctata exposed for 15, 30 and 45 days to untreated and microbially treated equalization tank effluent of textile industry.Untreated and microbially treated effluent exposed fish elicited alterations in blood, liver and gill tissuesHaematology, biochemical, histopathology and ultrastructural analysis resulted in massive pathologies in groups subjected to untreated effluent inducing maximum damage after 45 days of exposure.Less pronounced toxicity in fish C. punctata was observed in fish exposed to microbially treated effluent indicating its efficacy in toxicity reduction.

An evaluation of conventional and nature-based technologies for controlling antibiotic-resistant bacteria and antibiotic-resistant genes in wastewater treatment plants.

Antibiotic resistance is a globally recognized health concern which leads to longer hospital stays, increased morbidity, increased mortality, and higher medical costs. Understanding how antibiotic resistance persists and exchanges in environmental systems like soil, water, and wastewater are critically important for understanding the emergence of pathogens with new resistance profiles and the subsequent exposure of people who indirectly/directly come in contact with these pathogens. There are concerns about the widespread application of prophylactic antibiotics in the clinical and agriculture sectors, as well as chemicals/detergents used in food and manufacturing industries, especially the quaternary ammonium compounds which have been found responsible for the generation of resistant genes in water and soil. The rates of horizontal gene transfer increase where there is a lack of proper water/wastewater infrastructure, high antibiotic manufacturing industries, or endpoint users - such as hospitals and intensive agriculture. Conventional wastewater treatment technologies are often inefficient in the reduction of ARB/ARGs and provide the perfect combination of conditions for the development of antibiotic resistance. The wastewater discharged from municipal facilities may therefore be enriched with bacterial communities/pathogens and provide a suitable environment (due to the presence of nutrients and other pollutants) to enhance the transfer of antibiotic resistance. However, facilities with tertiary treatment (either traditional/emerging technologies) provide higher rates of reduction. This review provides a synthesis of the current understanding of wastewater treatment and antibiotic resistance, examining the drivers that may accelerate their possible transmission to a different environment, and highlighting the need for tertiary technologies used in treatment plants for the reduction of resistant bacteria/genes.

Metagenomics insights into microbiome and antibiotic resistance genes from free living amoeba in chlorinated wastewater effluents.

Free living amoeba (FLA) are among the organisms commonly found in wastewater and are well-established hosts for diverse microbial communities. Despite its clinical significance, there is little knowledge on the FLA microbiome and resistome, with previous studies relying mostly on conventional approaches. In this study we comprehensively analyzed the microbiome, antibiotic resistome and virulence factors (VFs) within FLA isolated from final treated effluents of two wastewater treatment plants (WWTPs) using shotgun metagenomics. Acanthamoeba has been identified as the most common FLA, followed by Entamoeba. The bacterial diversity showed no significant difference (p > 0.05) in FLA microbiomes obtained from the two WWTPs. At phylum level, the most dominant taxa were Proteobacteria, followed by Firmicutes and Actinobacteria. The most abundant genera identified were Enterobacter followed by Citrobacter, Paenibacillus, and Cupriavidus. The latter three genera are reported here for the first time in Acanthamoeba. In total, we identified 43 types of ARG conferring resistance to cephalosporins, phenicol, streptomycin, trimethoprim, quinolones, cephalosporins, tigecycline, rifamycin, and kanamycin. Similarly, a variety of VFs in FLA metagenomes were detected which included flagellar proteins, Type IV pili twitching motility proteins (pilH and rpoN), alginate biosynthesis genes AlgI, AlgG, AlgD and AlgW and Type VI secretion system proteins and general secretion pathway proteins (tssM, tssA, tssL, tssK, tssJ, fha, tssG, tssF, tssC and tssB, gspC, gspE, gspD, gspF, gspG, gspH, gspI, gspJ, gspK, and gspM). To the best of our knowledge, this is the first study of its kind to examine both the microbiomes and resistome in FLA, as well as their potential pathogenicity in treated effluents. Additionally, this study showed that FLA can host a variety of potentially pathogenic bacteria including Paenibacillus, and Cupriavidus that had not previously been reported, indicating that their relationship may play a role in the spread and persistence of antibiotic resistant bacteria (ARBs) and antibiotic resistance genes (ARGs) as well as the evolution of novel pathogens.

A Review of the Dissemination of Antibiotic Resistance through Wastewater Treatment Plants: Current Situation in Sri Lanka and Future Perspectives.

The emergence of antibiotic resistance (AR) poses a significant threat to both public health and aquatic ecosystems. Wastewater treatment plants (WWTPs) have been identified as potential hotspots for disseminating AR in the environment. However, only a limited number of studies have been conducted on AR dissemination through WWTPs in Sri Lanka. To address this knowledge gap in AR dissemination through WWTP operations in Sri Lanka, we critically examined the global situation of WWTPs as hotspots for transmitting antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) by evaluating more than a hundred peer-reviewed international publications and available national publications. Our findings discuss the current state of operating WWTPs in the country and highlight the research needed in controlling AR dissemination. The results revealed that the impact of different wastewater types, such as clinical, veterinary, domestic, and industrial, on the dissemination of AR has not been extensively studied in Sri Lanka; furthermore, the effectiveness of various wastewater treatment techniques in removing ARGs requires further investigation to improve the technologies. Furthermore, existing studies have not explored deeply enough the potential public health and ecological risks posed by AR dissemination through WWTPs.

Filtration columns containing waste iron shavings, loofah, and plastic shavings for further removal of nitrate and phosphate from wastewater effluent.

A novel pilot-scale advanced treatment system combining waste products as fillers is proposed and established to enhance the removal of nitrate (NO3--N) and phosphate (PO43--P) from secondary treated effluent. The system consists of four modular filter columns, one containing iron shavings (R1), two containing loofahs (R2 and R3), and one containing plastic shavings (R4). The monthly average concentration of total nitrogen (TN) and total phosphorus (TP) decreased from 8.87 to 2.52 mg/L and 0.607 to 0.299 mg/L, respectively. Micro-electrolysis of iron shavings produces Fe2+ and Fe3+ to remove PO43--P, while oxygen (O2) consumption creates anoxic conditions for subsequent denitrification. Gallionellaceae, iron-autotrophic Microorganisms, enriched the surface of iron shavings. The loofah served as a carbon source to remove NO3--N, and its porous mesh structure facilitated the attachment of biofilm. The plastic shavings intercepted suspended solids and degraded excess carbon sources. This system can be scaled up and installed at wastewater plants to improve the water quality of effluent cost-effectively.

Cost benefit analysis for green hydrogen production from treated effluent: The case study of Oman.

Recently, the management of water and wastewater is gaining attention worldwide as a way of conserving the natural resources on the planet. The traditional wastewater treatment in Oman is such that the treated effluent produced is only reused for unfeasible purposes such as landscape irrigation, cooling, or disposed of in the sea. Introducing more progressive reuse applications can result in achieving a circular economy by considering treated effluent as a source of producing new products. Accordingly, wastewater treatment plants can provide feedstock for green hydrogen production processes. The involvement of the wastewater industry in the green pathway of production scores major points in achieving decarbonization. In this paper, the technical and economic feasibility of green hydrogen production in Oman was carried out using a new technique that would help explore the benefits of the treated effluent from wastewater treatment in Oman. The feasibility study was conducted using the Al Ansab sewage treatment plant in the governate of Muscat in Wilayat (region), Bousher. The results have shown that the revenue from Al Ansab STP in a conventional case is 7.02 million OMR/year, while sustainable alternatives to produce hydrogen from the Proton Exchange Membrane (PEM) electrolyzer system for two cases with capacities of 1,500 kg H2/day and 50,000 kg H2/day, would produce revenue of 8.30 million OMR/year and 49.73 million OMR/year, respectively.

Temperature modulates the impacts of wastewater exposure on the physiology and behaviour of fathead minnow.

Municipal wastewater treatment plant (WWTP) effluent is a substantial source of pollution in aquatic habitats that can impact organisms across multiple levels of biological organization. Even though wastewater effluent is discharged continuously all year long, its impacts across seasons, specifically during winter, have largely been neglected in ecotoxicological research. Seasonal differences are of particular interest, as temperature-driven metabolic changes in aquatic organisms can significantly alter their ability to respond to chemical stressors. In this study, we examined the effects of multiple levels of wastewater effluent exposure (0, 25, or 50% treated effluent) on the physiological and behavioural responses of adult fathead minnow (Pimephales promelas) at temperatures simulating either summer (20 °C) or winter (4 °C) conditions. At 20 °C, wastewater exposure posed a metabolic cost to fish, demonstrated by higher standard metabolic rate and was associated with increased haematocrit and a reduction in boldness. In contrast, fish exposed to wastewater at 4 °C experienced no change in metabolic rate but performed fewer social interactions with their conspecifics. Taken together, our results demonstrate that wastewater exposure can lead to metabolic and behavioural disruptions, and such disruptions vary in magnitude and direction depending on temperature. Our findings highlight the importance of studying the interactions between stressors, while also underscoring the importance of research during colder periods of the year to broaden and deepen our understanding of the impacts of wastewater contamination in aquatic ecosystems.

Performance Efficiency of Conventional Treatment Plants and Constructed Wetlands towards Reduction of Antibiotic Resistance.

Domestic and industrial wastewater discharges harbor rich bacterial communities, including both pathogenic and commensal organisms that are antibiotic-resistant (AR). AR pathogens pose a potential threat to human and animal health. In wastewater treatment plants (WWTP), bacteria encounter environments suitable for horizontal gene transfer, providing an opportunity for bacterial cells to acquire new antibiotic-resistant genes. With many entry points to environmental components, especially water and soil, WWTPs are considered a critical control point for antibiotic resistance. The primary and secondary units of conventional WWTPs are not designed for the reduction of resistant microbes. Constructed wetlands (CWs) are viable wastewater treatment options with the potential for mitigating AR bacteria, their genes, pathogens, and general pollutants. Encouraging performance for the removal of AR (2-4 logs) has highlighted the applicability of CW on fields. Their low cost of construction, operation and maintenance makes them well suited for applications across the globe, especially in developing and low-income countries. The present review highlights a better understanding of the performance efficiency of conventional treatment plants and CWs for the elimination/reduction of AR from wastewater. They are viable alternatives that can be used for secondary/tertiary treatment or effluent polishing in combination with WWTP or in a decentralized manner.

Antibiotics and antibiotic resistant bacteria/genes in urban wastewater: A comparison of their fate in conventional treatment systems and constructed wetlands.

There is a growing concern that the use and misuse of antibiotics can increase the detection of antibiotic resistant genes (ARGs) in wastewater. Conventional wastewater treatment plants provide a pathway for ARGs and antibiotic resistant bacteria (ARB) to be released into natural water bodies. Research has indicated that conventional primary and secondary treatment systems can reduce ARGs/ARB to varying degrees. However, in developing/low-income countries, only 8-28% of wastewater is treated via conventional treatment processes, resulting in the environment being exposed to high levels of ARGs, ARB and pharmaceuticals in raw sewage. The use of constructed wetlands (CWs) has the potential to provide a low-cost solution for wastewater treatment, with respect to removal of nutrients, pathogens, ARB/ARGs either as a standalone treatment process or when integrated with conventional treatment systems. Recently, CWs have also been employed for the reduction of antibiotic residues, pharmaceuticals, and emerging contaminants. Given the benefits of ARG removal, low cost of construction, maintenance, energy requirement, and performance efficiencies, CWs offer a promising solution for developing/low-income countries. This review promotes a better understanding of the performance efficiency of treatment technologies (both conventional systems and CWs) for the reduction of antibiotics and ARGs/ARB from wastewater and explores workable alternatives.

Integrated ozonation assisted electrocoagulation process for the removal of cyanide from steel industry wastewater.

This work focuses on the removal of cyanide, chemical oxygen demand (COD), biological oxygen demand (BOD), and chloride from biological oxidation treated (BOT) effluent of the steel industry by integrated ozonation assisted electrocoagulation method. The removal efficiency of the pollutants was found to be inefficient when the electrocoagulation or ozonation process was performed separately. However, a combination of ozonation and electrocoagulation gives a highly satisfactory result. Such an integrated approach for the treatment of BOT effluent has not been previously investigated. The effects of operating variables viz. ozone generation rate, current density, and analysis time on pollutant removal were primarily analyzed for the hybrid process. The experimental operating condition was optimized and was seen that ozone generation rate of 1.33 mg s-1, ozonation time of 40 min, a current density of 100 A m-2, and electrolysis time of 30 min were sufficient for reducing the pollutant concentration below its permissible limits. The removal efficiencies of the combined process at optimum conditions were 99.8%, 94.7%, 95%, and 46.5% for cyanide, COD, BOD, and chloride ions, respectively. A kinetic study was performed for the degradation of the pollutants during ozonation. The pseudo-first-order kinetic model was found to be best suited for the analysis with the highest R2 value of 0.99 for cyanide, COD, BOD, and chloride, respectively. The mass transfer study conducted further showed that the volumetric mass transfer coefficient, Kla, was increased with that of the ozone generation rate. Cost estimation of the hybrid process was done and compared with that of the other reported integrated process.

Characteristics of refractory organics in industrial wastewater treated using a Fenton-coagulation process.

It is a challenging environmental issue to develop a cost-efficient approach for the removal of low-concentration refractory organics in industrial wastewater. In this study, the Fenton-coagulation process was utilised to remove the organics from the industrial effluent. The operational conditions of the Fenton-coagulation process were optimised, and then, the molecular weight (MW) and resin fraction distribution of dissolved organic matter (DOM) were investigated before and after the Fenton-coagulation process. The results showed that the efficiency of organic matter removal was affected by the Fe2+/H2O2 molar ratio, pH, and reaction time. The removal rate of chemical oxygen demand (COD) by Fenton-coagulation process reached 37.8% under the following conditions: pH = 4.0 - 5.0, H2O2 concentration = 34 mg/L, Fe2+/H2O2 molar ratio = 1.5, and reaction time = 120 min. The resin fraction distribution results showed that hydrophobic bases (HoB) were almost completely removed, and the removal rate of hydrophobic acids (HoA) reached 58%, while hydrophilic matter (HiM) became the dominant form in the final effluent after the Fenton-coagulation process due to the appearance of hydrophilic charged fractions (HiC). The results were explained by a two-step mechanism (Fenton oxidation and Fe3+ coagulation). According to the molecular weight (MW), 35.7% removal of the main fractions of organic matter with MW < 1 kDa was achieved. Furthermore, a pilot test proved that the final effluent quality after the Fenton-coagulation process conformed to the first class of the A discharge standard of pollutants for municipal wastewater treatment plants in Tianjin.

Operating Conditions Optimization via the Taguchi Method to Remove Colloidal Substances from Recycled Paper and Cardboard Production Wastewater.

Optimization of the ultrafiltration (UF) process to remove colloidal substances from a paper mill's treated effluent was investigated in this study. The effects of four operating parameters in a UF system (transmembrane pressure (TMP), cross-flow velocity (CFV), temperature and molecular weight cut-off (MWCO)) on the average permeate flux (Jv), organic matter chemical oxygen demand (COD) rejection rate and the cumulative flux decline (SFD), was investigated by robust experimental design using the Taguchi method. Analysis of variance (ANOVA) for an L9 orthogonal array were used to determine the significance of the individual factors, that is to say, to determine which factor has more and which less influence over the UF response variables. Analysis of the percentage contribution (P%) indicated that the TMP and MWCO have the greatest contribution to the average permeate flux and SFD. In the case of the COD rejection rate, the results showed that MWCO has the highest contribution followed by CFV. The Taguchi method and the utility concept were employed to optimize the multiple response variables. The optimal conditions were found to be 2.0 bar of transmembrane pressure, 1.041 m/s of the cross-flow velocity, 15 °C of the temperature, and 100 kDa MWCO. The validation experiments under the optimal conditions achieved Jv, COD rejection rate and SFD results of 81.15 L·m-2·h-1, 43.90% and 6.01, respectively. Additionally, SST and turbidity decreased by about 99% and 99.5%, respectively, and reduction in particle size from around 458-1281 nm to 12.71-24.36 nm was achieved. The field-emission scanning electron microscopy images under optimal conditions showed that membrane fouling takes place at the highest rate in the first 30 min of UF. The results demonstrate the validity of the approach of using the Taguchi method and utility concept to obtain the optimal membrane conditions for the wastewater treatment using a reduced number of experiments.

Retrospective nationwide occurrence of fipronil and its degradates in U.S. wastewater and sewage sludge from 2001 - 2016.

The insecticide fipronil is under regulatory scrutiny worldwide for its toxicity to pollinators and aquatic invertebrates. We conducted the first U.S. nationwide, longitudinal study of sewage sludges for fiproles, i.e., the sum of fipronil and its major degradates (fipronil sulfone, sulfide, amide, and desulfinyl). Archived sludges (n = 109) collected in three campaigns over 15 years were analyzed by isotope dilution liquid chromatography tandem mass spectrometry, revealing ubiquitous fiprole occurrence (0.2-385.3 µg/kg) since 2001 and a significant increase (2.4 ±â€¯0.3 fold; p < 0.005) both from 2001 to 2006/7 and from 2001 to 2015/6, but not a significant increase from 2006/7 to 2015/6 (p = 0.275). A geospatial analysis showed fiprole levels in municipal sludges to be uncoupled from agricultural use of fipronil on cropland surrounding sampled municipalities, thus pointing to non-agricultural uses (i.e., spot-on treatment and urban pest control) as a major source of fiprole loading to wastewater. Whereas anaerobic digestion was correlated with increases in fipronil sulfide at the expense of parental fipronil (p < 0.001), total fiprole levels in sewage sludges were similar regardless of the solids treatment approach applied (p = 0.519). Treatment plant effluent available from 12 facilities in 2015/6 contained fiproles at 0.3-112.9 ng/L, exceeding the United States Environmental Protection Agency (USEPA) aquatic invertebrate life benchmark for chronic fipronil exposure (11 ng/L) in 67% of cases. Whereas the USEPA identified fipronil in sludge only recently (2015), retrospective analyses and modeling conducted here show contaminant ubiquity and nationwide increases of fiprole mass (compared to 2001 levels) in U.S. municipal sludge (1140 ±â€¯230 kg in 2015/6), and treated effluent nationwide (1970 ±â€¯390 kg in 2015/6) over the past 15 years.

Biochemical response of indigenous microalgal consortia to variations in nitrogen concentration of treated effluent.

Cultivation conditions influence microalgal cellular components, such as lipid accumulation under nutrient depletion, high light irradiation and salinity stress. In this study, indigenous microalgal consortia were cultivated in batch mode using an actual treated effluent. The temporal response of cellular components to the variations in nitrogen concentration and influence of light irradiation on the response were investigated. Prolonged exposure of indigenous microalgal consortia to nitrogen exhaustion had minor effects on total lipid accumulation and enhancement of energy content. Nitrogen replenishment was followed by immediate crude protein accumulation for growth recovery. Total lipid reduction was observed under light and dark conditions after nitrogen replenishment. A one-day lag after nitrogen replenishment in the total lipid reduction was revealed under nitrogen depletion; meanwhile, under nitrogen exhaustion, lipids were utilised as the primary carbon and/or energy source after replenishment, as represented by the decrease from 10.8% to 9.04% within 6 h after the replenishment.

Synthesis of Petal-Like MnO2 Nanosheets on Hollow Fe3O4 Nanospheres for Heterogeneous Photocatalysis of Biotreated Papermaking Effluent.

Owing to the implementation of increasingly stringent water conservation policies and regulations, the pulp and paper mill industry must make increased efforts to meet the limits for pollutant emissions. The primary pretreatment and secondary biochemical treatment methods used currently generally fail to meet the country-specific environmental regulations, and the wastewater must be processed further even after being subjected to secondary biochemical treatments. In this work, we synthesized Fe3O4/MnO2 nanocomposites (FMNs) with a flower-like structure for use in the heterogeneous photocatalytic treatment of biotreated papermaking wastewater. FMNs1.25, which were formed using a KMnO4/Fe3O4 molar ratio of 1.25, could be separated readily using an external magnetic field and exhibited higher photocatalytic activity than those of the other samples as well as MnO2 and Fe3O4. The effects of various experimental parameters on the photocatalytic activity of FMNs1.25, including the initial pH of the wastewater and the catalyst dosage, were determined. The common chemical oxygen demand (CODCr) reduction rate in the case of this sample reached 56.58% within 120 min at a pH of 3, the CODCr of effluent after treatment was 52.10 mg/L. Further, even under neutral conditions, the CODCr of the treated effluent was below the current limit for discharge in China. Moreover, the nanocomposites exhibited good recyclability, and their catalytic activity did not decrease significantly even after five usage cycles. This study should serve as a platform for the fabrication of effective photocatalysts for the advanced treatment of biotreated papermaking effluent and refractory organic wastewater.

Adenovirus and rotavirus recovery from a treated effluent through an optimized skimmed-milk flocculation method.

Sewage treatment may be insufficient for the complete removal of enteric viruses, such as human adenoviruses (HAdV) and group A rotavirus (RVA). The differences in the efficiency of the treatment methodologies used may interfere with the detection of these viruses. The objective of this study was to optimize a skimmed-milk flocculation technique for the recovery of HAdV and RVA in the samples of treated effluent. The treated effluent collected at the wastewater treatment plant (WWTP) was processed via four protocols including modifications in the initial centrifugation step and the final concentration of skimmed-milk. The viral load and recovery rate were determined by quantitative PCR TaqMan® System. The highest recovery rates of HAdV, RVA, and bacteriophage PP7 (internal control process) were obtained when the concentration of skimmed-milk was doubled and no centrifugation step was used for the sample clarification. The optimized protocol was assessed in a field study conducted with 24 treated effluent samples collected bi-monthly during 2015. HAdV and RVA were detected in 50.0% (12/24) and 33.3% (08/24) of the samples tested, respectively, throughout the year, without seasonal variation (p > 0.05). This study corroborates the use of the organic flocculation method for virus recovery in environmental samples with the adaptation of the protocols to different aquatic matrices.

The impact of kraft pulping effluent on egg survival and hatching success in two species of Clupeiformes (Teleostei).

The anchoveta (Engraulis ringens) and sardine (Strangomera bentincki) are coastal pelagic species with important spawning areas off the coast of Chile. The discharge of secondary-treated effluents from a kraft pulp plant near one of these spawning areas has raised environmental concerns. Therefore, effluent effects on the development of anchoveta and sardine eggs were assessed by in vitro exposure. Eggs were sampled between 2007 and 2010 off Talcahuano, Chile. Subsequent toxicity tests (96 h duration, 12 °C) were performed using increasing effluent concentrations, a filtered seawater control, and two potassium dichromate concentrations (to verify consistent embryonic sensitivity). Egg mortality and hatching success were evaluated. For anchoveta, mortality (9.9 ± 7.1%) did not significantly differ among groups in five toxicity tests except the final toxicity test that showed significant differences in mortality (5.6% control vs 27.8% in 100% effluent). For sardines, no differences in mortality existed between the effluent dilutions (2.6 ± 3.6%) and control (6.3 ± 3.9%). Notably, anchoveta egg survival and hatching success rates were inconsistent, i.e., the highest rates of hatching failure occurred on the same sampling date with the highest rates of survival for the 100% effluent group (72%). In conclusion, the obtained results indicate that (i) anchoveta egg mortality and hatching failure increase only under 100% effluent exposure, coinciding with decreased egg quality near the end of spawning season and (ii) high effluent dilutions not significantly increase sardine and anchoveta egg mortalities. Nevertheless, the recorded adverse effects to the hatching process should be studied in greater detail, particularly considering interspecific variability and the complexity of reproductive processes, especially during early development.