Effect of household pipe materials on formation and chlorine resistance of the early-stage biofilm: various interspecific interactions exhibited by the same microbial biofilm in different pipe materials.

Microbial community biofilm exists in the household drinking water system and would pose threat to water quality. This paper explored biofilm formation and chlorination resistance of ten dual-species biofilms in three typical household pipes (stainless steel (SS), polypropylene random (PPR), and copper), and investigated the role of interspecific interaction. Biofilm biomass was lowest in copper pipes and highest in PPR pipes. A synergistic or neutralistic relationship between bacteria was evident in most biofilms formed in SS pipes, whereas four groups displayed a competitive relationship in biofilms formed in copper pipe. Chlorine resistance of biofilms was better in SS pipes and worse in copper pipes. It may be helped by interspecific relationships, but was more dependent on bacteria and resistance mechanisms such as more stable extracellular polymeric substance. The corrosion sites may also protect bacteria from chlorination. The findings provide useful insights for microbial control strategies in household drinking water systems.

Evaluation of the Safe Water Optimization Tool to Provide Evidence-Based Chlorination Targets in Surface Waters: Lessons from a Refugee Setting in Uganda.

The Safe Water Optimization Tool (SWOT) generates evidence-based point-of-distribution free residual chlorine (FRC) targets to adjust chlorine dosing by operators and ensure water quality at point-of-consumption. To investigate SWOT effectiveness in surface waters, we conducted two before-and-after mixed-method evaluations in a Uganda refugee settlement served by piped and trucked surface water systems. We surveyed 888 users on water knowledge, attitudes, and practices; collected 2768 water samples to evaluate FRC,Escherichia coli, and disinfection by-products (DBPs) concentrations; and conducted nine key-informant interviews with system operators about SWOT implementation. After baseline data collection, SWOT chlorination targets were generated, increasing point-of-distribution FRC targets from 0.2 to 0.7-0.8 mg/L and from 0.3 to 0.9 mg/L for piped and trucked systems, respectively. At endline, household point-of-consumption FRC ≥ 0.2 mg/L increased from 23 to 35% and from 8 to 42% in the two systems. With these increases, we did not observe increased chlorinated water rejection or DBPs concentrations exceeding international guidelines. Informants reported that SWOT implementation increased knowledge and capacity and improved operations. Overall, SWOT-generated chlorination targets increased chlorine dosage, which improved household water quality in surface waters although less than previously documented with groundwater sources. Additional operator support on prechlorination water treatment processes is needed to ensure maximally effective SWOT implementation for surface water sources.

Exploring correlations between microplastics, microorganisms, and water quality in an urban drinking water source.

The microplastic pollution in freshwater system is gradually becoming more severe, which has led to increasing attention on the distribution and potential harmful effects of microplastics. Moreover, microplastics may have an impact on river ecology and pose risks to ecosystems. Therefore, it is important to reveal this process. This study aimed to explore correlations between microplastics and free-living microorganisms in an urban drinking water source of Xiangjiang River by using multivariate statistical analysis. The results indicated that the abundance of microplastics (size 50 µm to 5 mm) in surface water and sediments ranged from 0.72 to 18.6 (mean ± SD: 7.32 ± 2.36) items L-1 and 26.3-302 (150 ± 75.6) items kg-1 dry weight (dw), respectively, suggesting potential microplastic pollution despite the protected status as a drinking water source. Higher microplastic abundances were observed in urban areas and the downstream of wastewater plants, with mostly granular shape, transparent and black color as well as 50-100 µm in size. The multivariate statistical analysis presented that the abundance of microplastics is not significantly correlated with water indicators, due to the complexity of the abundance data. The water indicators showed an obvious correlation with microplastics in colors of transparent and black, and smaller sizes of 50-100 µm. This is also true for microplastics and microorganisms in water and sediment. Proteobacteria was the main prokaryote in water and sediments, being positively correlated with 50-100 µm microplastics; while Chloroplastida was the dominated eukaryotes, presenting a weak correlation with smaller-size microplastics. Overall, when considering the properties of microplastics such as shape, color and size, the potential correlations with water indicators and microorganisms were more evident than abundance. This study provides new insights into the multivariate statistical analysis, explaining the potential correlations among microplastic properties, microorganisms and environmental factors in a river system.

The Phase Distribution Characteristics and Interphase Mass Transfer Behaviors of the CO2-Water/Saline System under Gathering and Transportation Conditions: Insights on Molecular Dynamics.

In order to investigate the interphase mass transfer and component distribution characteristics of the CO2-water system under micro-scale and nano-scale transport conditions, a micro-scale kinetic model representing interphase mass transfer in the CO2-water/saline system is developed in this paper. The molecular dynamics method is employed to delineate the diffusion and mass transfer processes of the system's components, revealing the extent of the effects of variations in temperature, pressure, and salt ion concentration on interphase mass transfer and component distribution characteristics. The interphase mass transfer process in the CO2-water system under transport conditions can be categorized into three stages: approach, adsorption, and entrance. As the system temperature rises and pressure decreases, the peak density of CO2 molecules at the gas-liquid interface markedly drops, with their aggregation reducing and their diffusion capability enhancing. The specific hydration structures between salt ions and water molecules hinder the entry of CO2 into the aqueous phase. Additionally, as the salt concentration in water increases, the density peak of CO2 molecules at the gas-liquid interface slightly increases, while the density value in the water phase region significantly decreases.

Research on the connectivity of urban river network-wetland and water quality simulation.

The connectivity of urban river networks plays an important role in cities in many aspects, such as urban water safety, water quality (WQ), and aquatic ecological balance. This study focuses on the river network and the Majiawan Wetland in the Chaoyang District of Beijing by establishing a two-dimensional hydrological WQ model employing various water allocation schemes between the river network and the wetland. Water circulation and WQ are the main indexes, and the effects of different scenarios on improving water circulation and WQ are simulated and compared. This study demonstrates that the addition of water replenishment at the intersection of river network and internal slow-water zones of the wetland (Scheme 2) has greater effectiveness in improving both hydrology and WQ compared to two other schemes. The water area of the Majiawan Wetland has expanded, and water velocity has increased. Using chemical oxygen demand, total nitrogen, and total phosphorus as the index values for determining the water class, the WQ of about 20% of the wetland area was reached Water Class II (domestic drinking water), with Water Class III (general industrial water) accounting for the other 80%. This study provides valuable evaluation and reference for similar areas of urban river network connectivity.

Microplastics in urban water systems, Tehran Metropolitan, Iran.

Urban water systems are potential sources of secondary microplastics (MPs) as well as a distributor of MPs in the environment. In the present study, the presence of MPs in the urban water systems of the Tehran Metropolitan (Capital of Iran) was investigated. In addition, the probable relationship of MPs with different land uses (i.e., residential-commercial, forest, military, and highway) was assessed. The results showed that all parts of Tehran's urban water system in the study area were contaminated with MPs (107.1 ± 39, 37.8 ± 10.5, 48.3 ± 3.1, 46.9 ± 5.6, 59.4 ± 26.5, 1.7, 2.0 ± 0.6, 7.9 ± 1, 1.8 ± 0.2 particles/liter at the residential, integrated, military, forest, highway runoffs, drinking water, groundwater, seasonal river, and the effluent of the wastewater treatment plants; respectively). However, significant differences were found between different land uses. As expected, the residential runoff had the highest rate of MPs pollution, with 107.1 ± 39 particles/liter. According to the obtained results and our estimation, more than five million MPs/day can enter into the water bodies and soil of the study area through the wastewater treatment plants. While there are significant differences in MPs in the different land uses, our findings suggest that residential areas and highways need further attention in controlling the spread of MPs in urban areas.

Incorporation of an Anion-Coordinated Triple Helicate into a Thin Film for Choline Recognition in an Aqueous System.

Functional thin films, being fabricated by incorporating discrete supramolecular architectures, have potential applications in research areas such as sensing, energy storage, catalysis, and optoelectronics. Here, we have determined that an anion-coordinated triple helicate can be solution-processed into a functional thin film by incorporation into a polymethyl methacrylate (PMMA) matrix. The thin films fabricated by the incorporation of the anion-coordinated triple helicate show multiple optical properties, such as fluorescence, CD, and CPL. In addition, the film has the ability to recognize choline and choline derivatives in a water system. The successful recognition of Ch+ by the film represents the first example of utilizing 'aniono'-supramolecular architectures for biomolecule detection in aqueous solution and opens up a new route for designing biocompatible functional materials.

Actor Roles and Networks in Implementing Urban Water Innovation: A Study of Onsite Water Reuse in the San Francisco Bay Area.

As climate change and rapid urbanization stress our aging water infrastructure, cities are under increasing pressure to develop more flexible, resilient, and modular water management systems. In response, onsite water reuse practices have been adopted by several cities globally. In addition to technological innovation, these novel water treatment systems also require new stakeholder collaborations, relationships, and processes to support them. There are, however, few models for stakeholder arrangements that support and encourage the adoption and success of such infrastructure. In this paper, we use interviews with stakeholders involved in onsite water reuse projects in the San Francisco Bay Area to create a social network map that describes the interactions between stakeholders at large and during specific phases of project implementation. Using qualitative content analysis of expert interviews and social network analysis, we identify four actor roles that are key to the functioning of this novel water infrastructure paradigm─specialists, continuity providers, program champions, and conveners─and discuss the importance of each role through the course of project implementation. These findings can be helpful for policy interventions and outreach efforts by other cities and communities looking to implement onsite water systems.

Mercury in multimedia system of Itacaiúnas Basin, Brazilian Amazon: An integrated approach to understand its distribution, origin, and ecological risk.

This study presents the first integrated study on total Hg (THg) level in surface soil (SS), bottom soil (BS), stream sediments (SD), lake sediments (LS), stream water (SW), and lake water (LW) of Itacaiúnas River Watershed (IRW), Brazil to investigate the source and distribution of Hg in different environmental media considering contrasts of geological domains and sub-basins and its potential ecological and human risk. Hg content in most of the soils and sediments were above the upper crustal average values (56 µg/kg), however, when compared to the legal limits set by the Resolution CONAMA (Conselho Nacional de Meio Ambiente: soil 500 µg/kg; sediment 486 µg/kg), only 1 soil sample from Parauapebas sub-basin and 4 sediment samples from Violão Lake exceeded the limit. None of the SW and LW samples (<0.2 µg/L; CONAMA limit for Class II freshwater) are markedly contaminated by Hg. The SS and BS show similar contents and spatial distribution of Hg with higher contents being registered mostly in the Itacaiúnas and Parauapebas sub-basins, which are closely correlated with SD. This suggests that Hg levels are largely of geogenic origin and anthropogenic effect is highly limited. Principal Component Analysis (PCA) results show that Hg is strongly associated with total organic carbon (TOC), loss on ignition (LOI), and SO3, indicating organic matter as the main factor controlling the distribution of Hg and this is the major cause of accentuated Hg enrichment in lake sediments. The ecological risk index revealed a low pollution risk for most of the solid samples, except 11% LS and <1.5% SS and SD samples, which registered moderate risk. Health risk assessment indicated no adverse non-carcinogenic health effect on either adults and children in terms of Hg contamination. This information will be useful for Hg risk assessment in the Carajás region and future environmental research in this direction in the Amazonia.

Water struggles and contested use: A capabilities assessment of household water security in marginalized communities.

In this study, we apply a capabilities approach to analyze a water consolidation project and water security outcomes following a severe drought in East Porterville, California. By combining hydro-social theory with the capabilities approach, we provide a holistic approach to household water security that is historically situated, considers residents' needs, and accounts for areas of life beyond hydration and domestic use. In addition, we offer a critical analysis of water system consolidation, a process of combining water systems physically and/or managerially as a solution to water insecurity in small towns. Drawing on interviews with residents, local experts, and government officials as well as archival research and participant observation, we find that the water consolidation project has mixed results for the East Porterville community, with beneficial, limiting, and contested effects on residents' social, cultural, and economic life. Although residents now have a consistent source of water in their homes, they find themselves limited in their ability to use water for drinking and cultural and economic purposes. Water negotiations and contestations also affected property values, independence, and livability. Through this empirical application of the capabilities approach, we demonstrate the need to expand the concept of water security and consolidation outcomes through needs-based perspectives. Furthermore, we show how the coupling of capabilities approach with a hydro-social framework provides descriptive, analytical, and explanatory tools for understanding and addressing household water security.

Anthropogenic factors affecting the Moskva River water quality: levels and sources of nutrients and potentially toxic elements in Moscow metropolitan area.

This study aims to identify the main patterns of distribution and sources of pollutants in the Moskva River and their influence on river water quality under different levels of anthropogenic stress caused by the largest megacity in Europe - Moscow. For this study, we determined concentrations of 18 trace elements, nutrient elements and major ions, chemical and biochemical oxygen demand, and physical parameters of water at 45 stations on the Moskva River and 20 stations on its tributaries during spring flood and low water of 2019 and 2020 to identify the extent and mechanisms of urban impact on its water chemistry. Chemical elements concentrations have been determined using ICP-MS and ICP-AES methods. Mn, Al, Cu, Sr, Zn, B, Mo, and inorganic nitrogen were outlined as key pollutants according to various drinking water and environmental guidelines. Using correlation and factor analysis, five groups of elements were identified, corresponding to different drivers controlling their unequal distribution within the watershed: mineral sources (Sr, Li, B, Mo, Ca), sewage and road runoff (TN, TP, Sb, Ni, N-NO2, BOD5, COD, V, Zn), impact of acidic wetlands (Al, COD, Zr, Bi), groundwater and landfills leachate (V, As, Pb, U, Sb), and industrial activities (Zn, Cu). Water quality in the Moskva Basin on the whole is good according to the CCME Water Quality Index. Local deterioration of water quality to marginal and even fair (depending on the reference water quality guideline) is confined to the Moskva River part downstream from the Kuryanovo aeration station to the Moskva mouth and to the mouths of several heavily contaminated tributaries.

Third party product certification for drinking water health effects.

Third-party certification to drinking water product consensus standards is how products for potable water systems are deemed suitable for public health and safety in North America. Drinking water product consensus standards are a type of standard developed through a process that includes participation from expert volunteers and requires general agreement from all stakeholders. Certification to drinking water product consensus standards is required via plumbing codes and state or local regulations in most of the United States and Canada, making third-party certification essential for products intended for sale and installation in North America. Third-party certification bodies (CBs) test and certify products to these drinking water product consensus standards through an evaluation process that includes a thorough review of each product's composition, laboratory testing, and inspection of each facility where the product is manufactured. Products that comply with the consensus standard requirements are entitled to bear a certification mark that demonstrates their suitability for use in potable water systems. Drinking water product standards developed by NSF reference NSF/ANSI/CAN 600: Health Effects Evaluation and Criteria for Chemicals in Drinking Water for the toxicological criteria to evaluate chemical leachates derived from material extraction testing. Here, we review the third-party product certification process for evaluating products used in potable water systems and describe how the certification process relies on the health effects criteria and toxicological evaluation procedures described in NSF/ANSI/CAN 600.

Development of methods based on low-temperature partitioning (LTP) for monitoring cresols and chlorophenols in sewage sludge, soil, and water in column leaching.

Cresols and chlorophenols are chemical contaminants that are potentially toxic to humans and can be found in sewage sludge. These chemical contaminants can migrate into the sludge-soil-water system when sludge is used as a conditioner for agricultural soils. Thus, the objective of this study was to develop methodologies based on extraction with low-temperature partitioning (LTP) to determine cresols and chlorophenols in sewage sludge, soil, and water. The analysis was performed by gas chromatography coupled with mass spectrometry (GC-MS). The validated methods were applied to monitor cresols and chlorophenols in a column-leaching study of a sludge-soil-water system. Satisfactory results were achieved for selectivity, limit of quantification (LOQ), linearity, accuracy, and precision. In the column leaching study, only 2,4,6-trichlorophenol was quantified in sludge samples after 20 days of the experiment. None of the studied compounds were quantified in soil and leached water samples, due to the degradation promoted by the microorganisms present in the sewage sludge. Finally, validated methods were suitable for monitoring cresols and chlorophenols in the sludge-soil-water system.

Complexities of the urban drinking water systems in Ethiopia and possible interventions for sustainability.

Rapid urbanization in developing countries has imposed threats and challenges to basic urban infrastructures like drinking water, transportation, and energy systems. The existing urban drinking water systems (UDWS) are highly stressed and unsustainable, particularly under changing hydroclimatic conditions, population growth, changing socioeconomic conditions, government decisions, and various policies. This study focuses on the complexities of UDWS in Sub-Saharan African countries, especially in Ethiopia. The objective of this study is to investigate the issues and challenges of urban drinking water systems (UDWS) in Ethiopia, specifically, to assess the gap between water supply and demand, water loss/non-revenue water, environmental, technical, institutional, and governance, etc. and propose sustainable interventions to deal with such issues so as to improve. For this purpose, a mix of methods involving primary data (including key informant interviews, field observations, and field measured data) and secondary data (including published articles, books, various reports, and design documents), as well as various computer-aided applications (mainly, ArcGIS and WaterGEMS) are used to collect data. The issues are deliberated through the UDWSs of Addis Ababa, Adama, Mekelle, and Dire Dawa cities in Ethiopia. Complexities like water shortage, high and low pressure in the water distribution network (WDN), non-revenue water (NRW)/water loss, source pollution, ineffective policies and governance, and weak institutions are the main challenges to Ethiopian cities' water utilities. Further, the case study noticed that in Addis Ababa alone, potable water is only accessible to 66% of the city population. A significant water supply deficit was observed in Mekelle city, where only half of the city population has access to potable water from the system. Additionally, in Addis Ababa, Adama, Mekelle, and Dire Dawa, above 35% of the freshwater produced is either NRW, unaccounted for, or lost, which is significantly higher than the upper 25% limit suggested by the World Bank. Therefore, it is recommended to adopt certain sustainable interventions, such as integrated water resource management, installing appurtenances like pressure-reducing valves, check valves in the WDN, controlling and monitoring of WDN through supervisory control and data acquisition and Internet of Things, effective and long-term planning and policy, etc. It is felt that the study will help the decision-makers and the operators of the UDWS utilities to run the water supply schemes in a sustainable manner. Supplementary Information: The online version contains supplementary material available at 10.1007/s10668-022-02901-7.

Observations from the Field Concerning Water and Dialysate for Hemodialysis.

This article describes survey observations and findings related to water and dialysate in hemodialysis facilities. Ensuring the quality of the water and dialysate is critically important for patient safety. Survey findings related to monitoring of the pH and conductivity, microbiology and disinfection, water system monitoring, water in the home dialysis setting, and quality assessment and performance improvement are reviewed. Implications for the nephrology registered nurse in each of the scenarios are discussed.

Forward-Looking Roadmaps for Long-Term Continuous Water Quality Monitoring: Bottlenecks, Innovations, and Prospects in a Critical Review.

Long-term continuous monitoring (LTCM) of water quality can bring far-reaching influences on water ecosystems by providing spatiotemporal data sets of diverse parameters and enabling operation of water and wastewater treatment processes in an energy-saving and cost-effective manner. However, current water monitoring technologies are deficient for long-term accuracy in data collection and processing capability. Inadequate LTCM data impedes water quality assessment and hinders the stakeholders and decision makers from foreseeing emerging problems and executing efficient control methodologies. To tackle this challenge, this review provides a forward-looking roadmap highlighting vital innovations toward LTCM, and elaborates on the impacts of LTCM through a three-hierarchy perspective: data, parameters, and systems. First, we demonstrate the critical needs and challenges of LTCM in natural resource water, drinking water, and wastewater systems, and differentiate LTCM from existing short-term and discrete monitoring techniques. We then elucidate three steps to achieve LTCM in water systems, consisting of data acquisition (water sensors), data processing (machine learning algorithms), and data application (with modeling and process control as two examples). Finally, we explore future opportunities of LTCM in four key domains, water, energy, sensing, and data, and underscore strategies to transfer scientific discoveries to general end-users.

Adoption of water reuse technologies: An assessment under different regulatory and operational scenarios.

Water reuse technologies may alleviate the water scarcity problems that affect many world regions, but their adoption is still limited. In particular, key actors in the adoption of water reuse technologies are water utilities, that provide both urban water and wastewater treatment services. Water utilities are embedded in the urban water system, which includes several stakeholders (urban water users, citizens at large, the environment) that may drive or pose barriers to water reuse adoption. Therefore, to ensure a smooth introduction of water reuse technologies, it is fundamental to understand how water reuse interacts with the existing urban water system and impacts its stakeholders. This paper contributes to the ongoing debate on water reuse by conceptualizing the interaction between water reuse technologies and the urban water system and its stakeholders, and addressing the adoption decision of water utilities by assessing its economic and environmental consequences. Based on a review of literature, policy and other secondary documents, and on primary data coming from interviews with experts from a water utility operating in Southern Italy, the study models the utility's response to a shift from urban to reuse water. It then simulates how reuse water introduction impacts on the utility and other stakeholders of the water system, under various regulatory and operational scenarios defined through a thorough analysis of policy documents and literature. Results show that the adoption of water reuse reduces the utility's margin by cannibalizing urban water demand, but appropriate policy measures may enhance the economic sustainability of reuse. System-level performances, such as impact on freshwater savings, costs for users, effects on the public budget, are also assessed, showing how different regulatory options moderate the intensity of impacts for the different stakeholders of the water system. Furthermore, the adoption of reuse water by the most distant users is found to enhance the economic sustainability of reuse and positively impact the utility's margin.

Novel Hydrophobic Polyvinyl-Alcohol Formaldehyde Sponges: Synthesis, Characterization, Fast and Effective Organic Solvent Uptake from Contaminated Soil Samples.

In the present research work, PVFTX-100, PVFSDS, and PVFT-80 sponges were prepared using polyvinyl-alcohol (PVA) with surfactants triton X-100/sodium dodecyl sulfate (SDS)/Tween 80, respectively, for the removal of organic solvents from polluted soil/water samples. All three obtained sponges were further made hydrophobic using dodecyltrimethoxysilane (DTMS). The prepared sponges were characterized using different spectroscopic techniques and SEM analysis. The peaks obtained near 1050 cm-1 and 790 cm-1 were attributed to Si-O-C and alkyl side chain C-H stretching vibration that confirmed the formation of desired sponges. The SEM images showed the random roughness with a number of protrusions on sponge surfaces, which further played an important role in the absorption and retention of organic solvents molecules. The Sears method was chosen to calculate the surface area and pore volume of all the synthesized sponge samples. Among all three prepared sponges, the PVFTX-100 sponge showed a high pore volume and large surface area, with a maximum percentage absorption capacity of 96%, 91%, 89.9%, 85.6%, and 80 for chlorobenzene, toluene, diesel, petrol, and hexane, respectively, after eightcycles. The organic solvent uptake using PVFTX-100, PVFSDS, and PVFT-80 sponges is quite a unique and simple technology, which could be employed at a large scale for contaminated soil/water systems.

Local Adaptation of Legionella pneumophila within a Hospital Hot Water System Increases Tolerance to Copper.

In large-building water systems, Legionella pneumophila is exposed to common environmental stressors such as copper. The aim of this study was to evaluate the susceptibility to copper of L. pneumophila isolates recovered from various sites: two clinical and seven environmental isolates from hot water system biofilm and water and from cooling tower water. After a 1-week acclimation in simulated drinking water, strains were exposed to various copper concentrations (0.8 to 5 mg/liter) for over 672 h. Complete loss of culturability was observed for three isolates following copper exposure to 5 mg/liter for 672 h. Two sequence type 1427 (ST1427)-like isolates were highly sensitive to copper, while the other two, isolated from biofilm samples, maintained higher culturability. The expression of the copper resistance gene copA evaluated by reverse transcription-quantitative PCR (RT-qPCR) was significantly higher for the biofilm isolates. All four ST1427-like isolates were recovered from the same water system during an outbreak. Whole-genome sequencing results confirmed that the four isolates are very close phylogenetically, differing by only 29 single nucleotide polymorphisms, suggesting in situ adaptation to microenvironmental conditions, possibly due to epigenetic regulation. These results indicate that the immediate environment within a building water distribution system influences the tolerance of L. pneumophila to copper. Increased contact of L. pneumophila biofilm strains with copper piping or copper alloys in the heat exchanger might lead to local adaptation. The phenotypic differences observed between water and biofilm isolates from the hot water system of a health care facility warrants further investigation to assess the relevance of evaluating disinfection performances based on water sampling alone.IMPORTANCELegionella pneumophila is a pathogen indigenous to natural and large building water systems in the bulk and the biofilm phases. The immediate environment within a system can impact the tolerance of L. pneumophila to environmental stressors, including copper. In health care facilities, copper levels in water can vary, depending on water quality, plumbing materials, and age. This study evaluated the impact of the isolation site (water versus biofilm, hot water system versus cooling tower) within building water systems. Closely related strains isolated from a health care facility hot water system exhibited variable tolerance to copper stress, shown by differential expression of copA, with biofilm isolates displaying highest expression and tolerance. Relying on the detection of L. pneumophila in water samples following exposure to environmental stressors such as copper may underestimate the prevalence of L. pneumophila, leading to inappropriate risk management strategies and increasing the risk of exposure for vulnerable patients.

Institutional Barriers to On-Site Alternative Water Systems: A Conceptual Framework and Systematic Analysis of the Literature.

Scientists are increasingly exploring on-site water systems to supplement conventional centralized water and wastewater infrastructure. While major technological advancements have been achieved, we still lack a systematic view on the non-technical, or institutional, elements that constitute important barriers to the uptake of on-site urban water management systems. This paper presents a conceptual framework distinguishing between institutional barriers in six key dimensions: Equity, Knowledge and Capabilities, Financial Investment, Legal and Regulatory Frameworks, Legitimacy, and Market Structures. The analysis of the existing literature covering these barriers is translated into a typology of the socio-technical complexity of different types of alternative water systems (e.g., non-potable reuse, rainwater systems, and nutrient recovery). Findings show that socio-technical complexity increases with the pollution load in the source water, correlating to potential health risk, and the number of sectors involved in the value chain of an alternative water system. For example, greywater reuse for toilet flushing might have systematically less complex institutional barriers than source separation for agricultural reuse. This study provides practitioners with easily accessible means of understanding non-technical barriers for various types of on-site reuse systems and provides researchers with a conceptual framework for capturing socio-technical complexity in the adoption of alternative water systems.