RESUMO
Secondary xylem and phloem originate from a lateral meristem called the vascular cambium that consists of one to several layers of meristematic cells. Recent lineage tracing studies have shown that only one of the cambial cells in each radial cell file functions as the stem cell, capable of producing both secondary xylem and phloem. Here, we first review how phytohormones and signalling peptides regulate vascular cambium formation and activity. We then propose how the stem cell concept, familiar from apical meristems, could be applied to cambium studies. Finally, we discuss how this concept could set the basis for future research.
Assuntos
Câmbio , Células-Tronco , Xilema , Câmbio/citologia , Câmbio/crescimento & desenvolvimento , Câmbio/fisiologia , Células-Tronco/citologia , Xilema/citologia , Floema/citologia , Reguladores de Crescimento de Plantas/metabolismo , Transdução de Sinais , Feixe Vascular de Plantas/crescimento & desenvolvimento , Feixe Vascular de Plantas/citologia , Meristema/citologia , Meristema/crescimento & desenvolvimentoRESUMO
A reduced rate of stem cell division is considered a widespread feature which ensures the integrity of genetic information during somatic development of plants and animals. Radial growth of plant shoots and roots is a stem cell-driven process that is fundamental for the mechanical and physiological support of enlarging plant bodies. In most dicotyledonous species, the underlying stem cell niche, the cambium, generates xylem inwards and phloem outwards. Despite the importance and intriguing dynamics of the cambium, the functional characterization of its stem cells is hampered by the lack of experimental tools for accessing distinct cambium sub-domains. Here, we use the hypocotyl of Arabidopsis thaliana to map stem cell activity in the proliferating cambium. Through pulse labeling and genetically encoded lineage tracing, we find that a single bifacial stem cell generates both xylem and phloem cell lineages. This cell is characterized by a specific combination of PXY (TDR), SMXL5 and WOX4 gene activity and a high division rate in comparison with tissue-specific progenitors. Our analysis provides a cellular fate map of radial plant growth, and suggests that stem cell quiescence is not a general prerequisite for life-long tissue production.This article has an associated 'The people behind the papers' interview.
Assuntos
Arabidopsis/crescimento & desenvolvimento , Câmbio/fisiologia , Floema/fisiologia , Células Vegetais/metabolismo , Desenvolvimento Vegetal/fisiologia , Células-Tronco/metabolismo , Xilema/fisiologia , Arabidopsis/citologia , Proteínas de Arabidopsis/biossíntese , Câmbio/citologia , Regulação da Expressão Gênica de Plantas/fisiologia , Hipocótilo/citologia , Hipocótilo/fisiologia , Floema/citologia , Raízes de Plantas/citologia , Raízes de Plantas/fisiologia , Células-Tronco/citologia , Xilema/citologiaRESUMO
In trees, lateral growth of the stem occurs through cell divisions in the vascular cambium. Vascular cambium activity is regulated by endogenous developmental programmes and environmental cues. However, the underlying mechanisms that regulate cambium activity are largely unknown. Genomic, biochemical and genetic approaches were used here to elucidate the role of PtrCLE20, a CLAVATA3 (CLV3)/embryo surrounding region (ESR)-related peptide gene, in the regulation of lateral growth in Populus. Fifty-two peptides encoded by CLE genes were identified in the genome of Populus trichocarpa. Among them PtrCLE20 transcripts were detected in developing xylem while the PtrCLE20 peptide was mainly localized in vascular cambium cells. PtrCLE20 acted in repressing vascular cambium activity indicated by that upregulation of PtrCLE20 resulted in fewer layers of vascular cambium cells with repressed expression of the genes related to cell dividing activity. PtrCLE20 peptide also showed a repression effect on the root growth of Populus and Arabidopsis, likely through inhibiting meristematic cell dividing activity. Together, the results suggest that PtrCLE20 peptide, produced from developing xylem cells, plays a role in regulating lateral growth by repression of cambium activity in trees.
Assuntos
Câmbio/fisiologia , Peptídeos/fisiologia , Populus/genética , Xilema/fisiologia , Regulação da Expressão Gênica de Plantas , Populus/crescimento & desenvolvimentoRESUMO
Tree stems are an overlooked source of volatile organic compounds (VOCs). Their contribution to ecosystem processes and total VOC fluxes is not well studied, and assessing it requires better understanding of stem emission dynamics and their driving processes. To gain more mechanistic insight into stem emission patterns, we measured monoterpene, methanol and acetaldehyde emissions from the stems of mature Scots pines (Pinus sylvestris L.) in a boreal forest over three summers. We analysed the effects of temperature, soil water content, tree water status, transpiration and growth on the VOC emissions and used generalized linear models to test their relative importance in explaining the emissions. We show that Scots pine stems are considerable sources of monoterpenes, methanol and acetaldehyde, and their emissions are strongly regulated by temperature. However, even small changes in water availability affected the emission potentials: increased soil water content increased the monoterpene emissions within a day, whereas acetaldehyde and methanol emissions responded within 2-4 days. This lag corresponded to their transport time in the xylem sap from the roots to the stem. Moreover, the emissions of monoterpenes, methanol and acetaldehyde were influenced by the cambial growth rate of the stem with 6-10-day lags.
Assuntos
Acetaldeído/metabolismo , Câmbio/metabolismo , Metanol/metabolismo , Monoterpenos/metabolismo , Pinus sylvestris/metabolismo , Caules de Planta/metabolismo , Câmbio/crescimento & desenvolvimento , Câmbio/fisiologia , Pinus sylvestris/crescimento & desenvolvimento , Pinus sylvestris/fisiologia , Água/metabolismoRESUMO
Heat injuries sustained in a fire can initiate a cascade of complex mechanisms that affect the physiology of trees after fires. Uncovering the exact physiological mechanisms and relating specific injuries to whole-plant and ecosystem functioning is the focus of intense current research. Recent studies have made critical steps forward in our understanding of tree physiological processes after fires, and have suggested mechanisms by which fire injuries may interact with disturbances such as drought, insects and pathogens. We outline a conceptual framework that unifies the involved processes, their interconnections, and possible feedbacks, and contextualizes these responses with existing hypotheses for disturbance effects on plants and ecosystems. By focusing on carbon and water as currencies of plant functioning, we demonstrate fire-induced cambium/phloem necrosis and xylem damage to be main disturbance effects. The resulting carbon starvation and hydraulic dysfunction are linked with drought and insect impacts. Evaluating the precise process relationships will be crucial for fully understanding how fires can affect tree functionality, and will help improve fire risk assessment and mortality model predictions. Especially considering future climate-driven increases in fire frequency and intensity, knowledge of the physiological tree responses is important to better estimate postfire ecosystem dynamics and interactions with climate disturbances.
Assuntos
Incêndios , Árvores/fisiologia , Água , Câmbio/fisiologia , Impedância Elétrica , Xilema/fisiologiaRESUMO
Tissue regeneration upon wounding in plants highlights the developmental plasticity of plants. Previous studies have described the morphological and molecular changes of secondary vascular tissue (SVT) regeneration after large-scale bark girdling in trees. However, how phytohormones regulate SVT regeneration is still unknown. Here, we established a novel in vitro SVT regeneration system in the hybrid aspen (Populus tremula × Populus tremuloides) clone T89 to bypass the limitation of using field-grown trees. The effects of phytohormones on SVT regeneration were investigated by applying exogenous hormones and utilizing various transgenic trees. Vascular tissue-specific markers and hormonal response factors were also examined during SVT regeneration. Using this in vitro regeneration system, we demonstrated that auxin and cytokinin differentially regulate phloem and cambium regeneration. Whereas auxin is sufficient to induce regeneration of phloem prior to continuous cambium restoration, cytokinin only promotes the formation of new phloem, not cambium. The positive role of cytokinin on phloem regeneration was further confirmed in cytokinin overexpression trees. Analysis of a DR5 reporter transgenic line further suggested that cytokinin blocks the re-establishment of auxin gradients, which is required for the cambium formation. Investigation on the auxin and cytokinin signalling genes indicated these two hormones interact to regulate SVT regeneration. Taken together, the in vitro SVT regeneration system allows us to make use of various molecular and genetic tools to investigate SVT regeneration. Our results confirmed that complementary auxin and cytokinin domains are required for phloem and cambium reconstruction.
Assuntos
Citocininas/metabolismo , Ácidos Indolacéticos/metabolismo , Feixe Vascular de Plantas/fisiologia , Populus/fisiologia , Regeneração/fisiologia , Árvores/fisiologia , Câmbio/fisiologia , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Modelos Biológicos , Floema/fisiologia , Populus/genética , Árvores/genéticaRESUMO
Xylem vessel structure changes as trees grow and mature. Age- and development-related changes in xylem structure are likely related to changes in hydraulic function. We examined whether hydraulic function, including hydraulic conductivity and vulnerability to water-stress-induced xylem embolism, changed over the course of cambial development in the stems of 17 tree species. We compared current-year growth of young (1-4 years), intermediate (2-7 years), and older (3-10 years) stems occurring in series along branches. Diffuse and ring porous species were examined, but nearly all species produced only diffuse porous xylem in the distal branches that were examined irrespective of their mature xylem porosity type. Vessel diameter and length increased with cambial age. Xylem became both more conductive and more cavitation resistant with cambial age. Ring porous species had longer and wider vessels and xylem that had higher conductivity and was more vulnerable to cavitation; however, these differences between porosity types were not present in young stem samples. Understanding plant hydraulic function and architecture requires the sampling of multiple-aged tissues because plants may vary considerably in their xylem structural and functional traits throughout the plant body, even over relatively short distances and closely aged tissues.
Assuntos
Câmbio/anatomia & histologia , Madeira/anatomia & histologia , Xilema/anatomia & histologia , Câmbio/fisiologia , Caules de Planta , Árvores/anatomia & histologia , Árvores/fisiologia , Água/fisiologia , Madeira/fisiologia , Xilema/fisiologiaRESUMO
PREMISE: Cambial activity in some tropical trees varies intra-annually, with the formation of xylem rings. Identification of the climatic factors that regulate cambial activity is important for understanding the growth of such species. We analyzed the relationship between climatic factors and cambial activity in four tropical hardwoods, Acacia mangium, Tectona grandis, Eucalyptus urophylla, and Neolamarckia cadamba in Yogyakarta, Java Island, Indonesia, which has a rainy season (November-June) and a dry season (July-October). METHODS: Small blocks containing phloem, cambium, and xylem were collected from main stems in January 2014, October 2015 and October 2016, and examined with light microscopy for cambial cell division, fusiform cambial cells, and expanding xylem cells as evidence of cambial activity. RESULTS: During the rainy season, when precipitation was high, cambium was active. By contrast, during the dry season in 2015, when there was no precipitation, cambium was dormant. However, in October 2016, during the so-called dry season, cambium was active, cell division was conspicuous, and a new xylem ring formation was initiated. The difference in cambial activity appeared to be related to an unusual pattern of precipitation during the typically dry months, from July to October, in 2016. CONCLUSIONS: Our results indicate that low or absent precipitation for 3 to 4 months induces cessation of cambial activity and temporal periodicity of wood formation in the four species studied. By contrast, in the event of continuing precipitation, cambial activity in the same trees may continue throughout the year. The frequency pattern of precipitation appears to be an important determinant of wood formation in tropical trees.
Assuntos
Câmbio/anatomia & histologia , Câmbio/fisiologia , Chuva , Árvores/anatomia & histologia , Árvores/fisiologia , Acacia/anatomia & histologia , Acacia/crescimento & desenvolvimento , Acacia/fisiologia , Câmbio/crescimento & desenvolvimento , Divisão Celular , Eucalyptus/anatomia & histologia , Eucalyptus/crescimento & desenvolvimento , Eucalyptus/fisiologia , Agricultura Florestal , Indonésia , Lamiaceae/anatomia & histologia , Lamiaceae/crescimento & desenvolvimento , Lamiaceae/fisiologia , Rubiaceae/anatomia & histologia , Rubiaceae/crescimento & desenvolvimento , Rubiaceae/fisiologia , Estações do Ano , Especificidade da Espécie , Árvores/crescimento & desenvolvimentoRESUMO
Trees are carbon dioxide sinks and major producers of terrestrial biomass with distinct seasonal growth patterns. Circadian clocks enable the coordination of physiological and biochemical temporal activities, optimally regulating multiple traits including growth. To dissect the clock's role in growth, we analysed Populus tremula × P. tremuloides trees with impaired clock function due to down-regulation of central clock components. late elongated hypocotyl (lhy-10) trees, in which expression of LHY1 and LHY2 is reduced by RNAi, have a short free-running period and show disrupted temporal regulation of gene expression and reduced growth, producing 30-40% less biomass than wild-type trees. Genes important in growth regulation were expressed with an earlier phase in lhy-10, and CYCLIN D3 expression was misaligned and arrhythmic. Levels of cytokinins were lower in lhy-10 trees, which also showed a change in the time of peak expression of genes associated with cell division and growth. However, auxin levels were not altered in lhy-10 trees, and the size of the lignification zone in the stem showed a relative increase. The reduced growth rate and anatomical features of lhy-10 trees were mainly caused by misregulation of cell division, which may have resulted from impaired clock function.
Assuntos
Divisão Celular/genética , Relógios Circadianos/genética , Citocininas/metabolismo , Regulação da Expressão Gênica de Plantas , Populus/crescimento & desenvolvimento , Populus/genética , Árvores/crescimento & desenvolvimento , Árvores/genética , Biomassa , Câmbio/fisiologia , Ácidos Indolacéticos/metabolismo , Lignina/metabolismo , Metaboloma , Metabolômica , Mutação/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Populus/citologia , Ligação Proteica , Interferência de RNA , Árvores/citologiaRESUMO
Angiosperm trees reorient their woody stems by asymmetrically producing a specialized xylem tissue, tension wood, which exerts a strong contractile force resulting in negative gravitropism of the stem. Here, we show, in Populus trees, that initial gravity perception and response occurs in specialized cells through sedimentation of starch-filled amyloplasts and relocalization of the auxin transport protein, PIN3. Gibberellic acid treatment stimulates the rate of tension wood formation and gravibending and enhances tissue-specific expression of an auxin-responsive reporter. Gravibending, maturation of contractile fibers, and gibberellic acid (GA) stimulation of tension wood formation are all sensitive to transcript levels of the Class I KNOX homeodomain transcription factor-encoding gene ARBORKNOX2 (ARK2). We generated genome-wide transcriptomes for trees in which gene expression was perturbed by gravistimulation, GA treatment, and modulation of ARK2 expression. These data were employed in computational analyses to model the transcriptional networks underlying wood formation, including identification and dissection of gene coexpression modules associated with wood phenotypes, GA response, and ARK2 binding to genes within modules. We propose a model for gravitropism in the woody stem in which the peripheral location of PIN3-expressing cells relative to the cambium results in auxin transport toward the cambium in the top of the stem, triggering tension wood formation, while transport away from the cambium in the bottom of the stem triggers opposite wood formation.
Assuntos
Gravitropismo/genética , Reguladores de Crescimento de Plantas/metabolismo , Populus/genética , Câmbio/citologia , Câmbio/genética , Câmbio/fisiologia , Perfilação da Expressão Gênica , Giberelinas/metabolismo , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Ácidos Indolacéticos/metabolismo , Especificidade de Órgãos , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Caules de Planta/citologia , Caules de Planta/genética , Caules de Planta/fisiologia , Plantas Geneticamente Modificadas , Plastídeos/genética , Plastídeos/fisiologia , Populus/citologia , Populus/fisiologia , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Árvores , Madeira/citologia , Madeira/genética , Madeira/fisiologia , Xilema/genética , Xilema/fisiologiaRESUMO
The plant vascular system, composed of xylem and phloem, evolved to connect plant organs and transport various molecules between them. During the post-embryonic growth, these conductive tissues constitutively form from cells that are derived from a lateral meristem, commonly called procambium and cambium. Procambium/cambium contains pluripotent stem cells and provides a microenvironment that maintains the stem cell population. Because vascular plants continue to form new tissues and organs throughout their life cycle, the formation and maintenance of stem cells are crucial for plant growth and development. In this decade, there has been considerable progress in understanding the molecular control of the organization and maintenance of stem cells in vascular plants. Noticeable advance has been made in elucidating the role of transcription factors and major plant hormones in stem cell maintenance and vascular tissue differentiation. These studies suggest the shared regulatory mechanisms among various types of plant stem cell pools. In this review, we focus on two aspects of stem cell function in the vascular cambium, cell proliferation and cell differentiation.
Assuntos
Floema/crescimento & desenvolvimento , Células-Tronco/fisiologia , Xilema/crescimento & desenvolvimento , Câmbio/citologia , Câmbio/genética , Câmbio/metabolismo , Câmbio/fisiologia , Diferenciação Celular/genética , Proliferação de Células , Regulação da Expressão Gênica de Plantas , Meristema/citologia , Meristema/genética , Meristema/crescimento & desenvolvimento , Meristema/fisiologia , Modelos Biológicos , Floema/citologia , Floema/genética , Floema/fisiologia , Células Vegetais/metabolismo , Células Vegetais/fisiologia , Desenvolvimento Vegetal/genética , Desenvolvimento Vegetal/fisiologia , Células-Tronco/metabolismo , Xilema/citologia , Xilema/genética , Xilema/fisiologiaRESUMO
Here, we summarize studies on the effects of elevated [CO2 ] (CO2e ) on the structure and function of plant hydraulic architecture and explore the implications of those changes using a model. Changes in conduit diameter and hydraulic conductance due to CO2e vary among species. Ring-porous species tend towards an increase in conduit size and consequently conductivity. The effect in diffuse-porous species is much more limited. In conifers, the results are mixed, some species show minor changes in xylem structure, while other studies found increases in tracheid density and diameter. Non-woody plants generally exhibited the reverse pattern with narrower conduits and lower hydraulic conductivity under CO2e . Further, changes in drought-resistance traits suggest that non-woody plants were the most affected by CO2e , which may permit them to better resist drought-induced embolism under future conditions. Due to their complexity, acclimation in hydraulic traits in response to CO2e is difficult to interpret when relying solely on measurements. When we examined how the observed tissues-specific trends might alter plant function, our modelling results suggest that these hydraulic changes would lead to reduced conductance and more frequent drought stress in trees that develop under CO2e with a more pronounced effect in isohydric than in anisohydric species.
Assuntos
Dióxido de Carbono/metabolismo , Secas , Fenômenos Fisiológicos Vegetais , Água/metabolismo , Aclimatação , Atmosfera , Câmbio/genética , Câmbio/fisiologia , Desidratação , Regulação da Expressão Gênica de Plantas , Desenvolvimento Vegetal , Estômatos de Plantas/fisiologia , Transpiração Vegetal/fisiologia , Solo/química , Árvores/fisiologia , Xilema/anatomia & histologiaRESUMO
Secondary growth occurs in dicotyledons and gymnosperms, and results in an increased girth of plant organs. It is driven primarily by the vascular cambium, which produces thousands of cells throughout the life of several plant species. For instance, even in the small herbaceous model plant Arabidopsis, manual quantification of this massive process is impractical. Here, we provide a comprehensive overview of current methods used to measure radial growth. We discuss the issues and problematics related to its quantification. We highlight recent advances and tools developed for automated cellular phenotyping and its future applications.
Assuntos
Desenvolvimento Vegetal , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/fisiologia , Botânica/métodos , Câmbio/citologia , Câmbio/crescimento & desenvolvimento , Câmbio/fisiologia , Desenvolvimento Vegetal/fisiologiaRESUMO
Vascular tissue, comprising xylem and phloem, is responsible for the transport of water and nutrients throughout the plant body. Such tissue is continually produced from stable populations of stem cells, specifically the procambium during primary growth and the cambium during secondary growth. As the majority of plant biomass is produced by the cambium, there is an obvious demand for an understanding of the genetic mechanisms that control the rate of vascular cell division. Moreover, wood is an industrially important product of the cambium, and research is beginning to uncover similar mechanisms in trees such as poplar. This review focuses upon recent work that has identified the major molecular pathways that regulate procambial and cambial activity.
Assuntos
Câmbio/crescimento & desenvolvimento , Divisão Celular/fisiologia , Câmbio/fisiologia , Floema/citologia , Floema/crescimento & desenvolvimento , Floema/fisiologia , Reguladores de Crescimento de Plantas/fisiologia , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/fisiologia , Caules de Planta/crescimento & desenvolvimento , Caules de Planta/fisiologia , Xilema/citologia , Xilema/crescimento & desenvolvimento , Xilema/fisiologiaRESUMO
Perennial trees have the amazing ability to adjust their growth rate to both adverse and favorable seasonally reoccurring environmental conditions over hundreds of years. In trunks and stems, the basis for the tuning of seasonal growth rate is the regulation of cambial stem cell activity. Cambial stem cell quiescence and dormancy protect the tree from potential physiological and genomic damage caused by adverse growing conditions and may permit a long lifespan. Cambial dormancy and longevity are both aspects of a tree's life for which the study of cambial stem cell behavior in the annual model plant Arabidopsis is inadequate. Recent functional analyses of hormone perception and catabolism mutants in Populus indicate that shoot-derived long-range signals, as well as local cues, steer cambial activity. Auxin is central to the regulation of cambial activity and probably also maintenance. Emerging genome editing and phenotyping technologies will enable the identification of down-stream targets of hormonal action and facilitate the genetic dissection of complex traits of cambial biology.
Assuntos
Câmbio/citologia , Reguladores de Crescimento de Plantas/fisiologia , Arabidopsis/citologia , Arabidopsis/fisiologia , Câmbio/fisiologia , Meio Ambiente , Regulação da Expressão Gênica de Plantas/fisiologia , Caules de Planta/citologia , Caules de Planta/fisiologia , Estações do Ano , Árvores/citologia , Árvores/crescimento & desenvolvimento , Árvores/fisiologiaRESUMO
In plants, the cambium and procambium are meristems from which vascular tissue is derived. In contrast to most plant cells, stem cells within these tissues are thin and extremely long. They are particularly unusual as they divide down their long axis in a highly ordered manner, parallel to the tangential axis of the stem. CLAVATA3-LIKE/ESR-RELATED 41 (CLE41) and PHLOEM INTERCALATED WITH XYLEM (PXY) are a multifunctional ligand-receptor pair that regulate vascular cell division, vascular organisation and xylem differentiation in vascular tissue. A transcription factor gene, WUSCHEL HOMEOBOX RELATED 4 (WOX4) has been shown to act downstream of PXY. Here we show that WOX4 acts redundantly with WOX14 in the regulation of vascular cell division, but that these genes have no function in regulating vascular organisation. Furthermore, we identify an interaction between PXY and the receptor kinase ERECTA (ER) that affects the organisation of the vascular tissue but not the rate of cell division, suggesting that cell division and vascular organisation are genetically separable. Our observations also support a model whereby tissue organisation and cell division are integrated via PXY and ER signalling, which together coordinate development of different cell types that are essential for normal stem formation.
Assuntos
Proteínas de Arabidopsis/fisiologia , Câmbio/fisiologia , Proteínas de Homeodomínio/fisiologia , Proteínas Quinases/fisiologia , Proteínas Serina-Treonina Quinases/fisiologia , Receptores de Superfície Celular/fisiologia , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Divisão Celular , Membrana Celular/metabolismo , Cruzamentos Genéticos , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Proteínas de Homeodomínio/genética , Ligantes , Mutação , Fenótipo , Floema/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Receptores de Superfície Celular/metabolismo , Transdução de Sinais , Xilema/metabolismoRESUMO
The xylem and phloem, major conducting and supporting tissues in vascular plants, are established by cell division and cell-type specification in the procambium/cambium. The organization of the xylem, phloem, and procambium/cambium is tightly controlled. However, the underlying regulatory mechanisms remain largely unknown. In this study, we report the discovery of two transcription factors, AT-HOOK MOTIF NUCLEAR LOCALIZED PROTEIN 3 (AHL3) and AHL4, which regulate vascular tissue boundaries in Arabidopsis thaliana roots. In either of the knockout mutants of AHL3 and AHL4, encoding closely related AT-hook transcription factors, a misspecification of tissue boundaries between the xylem and procambium occurred and ectopic xylem developed in the procambium domain. In plants, specific types of transcription factors can serve as direct intercellular signals by moving from one cell to another, playing crucial roles in tissue patterning. Adding to this paradigm, AHL4 moves actively from the procambium to xylem in the root meristem to regulate the tissue boundaries. When the intercellular movement of AHL4 was impaired, AHL4 could not complement the xylem phenotype in the ahl4. Furthermore, AHL4 revealed unique characteristics in that it interacts with AHL3 in vivo and that this interaction facilitates their intercellular trafficking. Taken together, this study uncovered a novel mechanism in vascular tissue patterning that requires the intercellular trafficking of two interacting transcription factors.
Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/citologia , Regulação da Expressão Gênica de Plantas , Raízes de Plantas/citologia , Feixe Vascular de Plantas/citologia , Motivos AT-Hook , Arabidopsis/genética , Arabidopsis/fisiologia , Proteínas de Arabidopsis/genética , Padronização Corporal , Câmbio/citologia , Câmbio/genética , Câmbio/fisiologia , Comunicação Celular , Diferenciação Celular , Movimento Celular , Técnicas de Inativação de Genes , Microscopia Confocal , Mutação , Especificidade de Órgãos , Fenótipo , Floema/citologia , Floema/genética , Floema/fisiologia , Epiderme Vegetal/citologia , Epiderme Vegetal/genética , Epiderme Vegetal/fisiologia , Folhas de Planta/citologia , Folhas de Planta/genética , Folhas de Planta/fisiologia , Raízes de Plantas/genética , Raízes de Plantas/fisiologia , Feixe Vascular de Plantas/genética , Feixe Vascular de Plantas/fisiologia , Transporte Proteico , Proteínas Recombinantes de Fusão , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Técnicas do Sistema de Duplo-Híbrido , Xilema/citologia , Xilema/genética , Xilema/fisiologiaRESUMO
Cell-to-cell communication is a fundamental mechanism for coordinating developmental and physiological events in multicellular organisms. Heterotrimeric G proteins are key molecules that transmit extracellular signals; similarly, CLAVATA signaling is a crucial regulator in plant development. Here, we show that Arabidopsis thaliana Gß mutants exhibit an enlarged stem cell region, which is similar to that of clavata mutants. Our genetic and cell biological analyses suggest that the G protein beta-subunit1 AGB1 and RPK2, one of the major CLV3 peptide hormone receptors, work synergistically in stem cell homeostasis through their physical interactions. We propose that AGB1 and RPK2 compose a signaling module to facilitate meristem development.
Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Câmbio/metabolismo , Proliferação de Células , Subunidades beta da Proteína de Ligação ao GTP/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Proteínas de Arabidopsis/genética , Câmbio/fisiologia , Subunidades beta da Proteína de Ligação ao GTP/genética , Ligação Proteica , Proteínas Serina-Treonina Quinases/genética , Transdução de SinaisRESUMO
BACKGROUND AND AIMS: In temperate regions, trees undergo annual cycles of cambial growth, with periods of cambial activity and dormancy. Environmental factors might regulate the cambial growth, as well as the development of cambial derivatives. We investigated the effects of low temperature by localized cooling on cambial activity and latewood formation in two conifers, Chamaecyparis obtusa and Cryptomeria japonica. METHODS: A plastic rubber tube that contained cooled water was wrapped around a 30-cm-wide portion of the main stem of Chamaecyparis obtusa and Cryptomeria japonica trees during seasons of active cambium. Small blocks were collected from both cooled and non-cooled control portions of the stems for sequential observations of cambial activity and for anatomical measurements of cell morphology by light microscopy and image analysis. KEY RESULTS: The effect of localized cooling was first observed on differentiating tracheids. Tracheids narrow in diameter and with significantly decreased cambial activity were evident 5 weeks after the start of cooling in these stems. Eight weeks after the start of cooling, tracheids with clearly diminished diameters and thickened cell walls were observed in these stems. Thus, localized low temperature induced narrow diameters and obvious thickening of secondary cell walls of tracheids, which were identified as latewood tracheids. Two months after the cessation of cooling, a false annual ring was observed and cambium became active again and produced new tracheids. In Cryptomeria japonica, cambial activity ceased earlier in locally cooled portions of stems than in non-cooled stems, indicating that the cambium had entered dormancy sooner in the cooled stems. CONCLUSIONS: Artificial cooling of stems induced latewood formation and cessation of cambial activity, indicating that cambium and its derivatives can respond directly to changes in temperature. A decrease in the temperature of the stem is a critical factor in the control of cambial activity and xylem differentiation in trees.
Assuntos
Câmbio/fisiologia , Temperatura Baixa , Caules de Planta/fisiologia , Estações do Ano , Traqueófitas/fisiologia , Madeira/fisiologia , Ar , Chamaecyparis/fisiologia , Cryptomeria/fisiologiaRESUMO
Vessel grouping in angiosperms may improve hydraulic integration and increase the spread of cavitations through redundancy pathways. Although disputed, it is increasingly attracting research interest as a potentially significant hydraulic trait. However, the variation of vessel grouping in a tree is poorly understood. I measured the number of solitary and grouped vessels in the xylem of Betula platyphylla Roth. from the pith to the bark along the water flow path. The vessel grouping parameters included the mean number of vessels per vessel group (VG), percentage of solitary vessels (SVP), percentage of radial multiple vessels (MVP), and percentage of cluster vessels (CVP). The effects of cambial age (CA) and flow path-length (PL) on the vessel grouping were analyzed using a linear mixed model.VG and CVP increased nonlinearly, SVP decreased nonlinearly with PL. In trunks and branches, VG and CVP decreased nonlinearly, and SVP increased nonlinearly with CA. In roots, the parameters had no change with CA. MVP was almost constant with PL or CA. The results suggest that vessel grouping has a nonrandom variation pattern, which is affected deeply by cambial age and water flow path.