Physiological of biochar and α-Fe2O3 nanoparticles as amendments of Cd accumulation and toxicity toward muskmelon grown in pots.

BACKGROUND: Due to the severe cadmium (Cd) pollution of farmland soil, effective measures need to be taken to reduce the Cd content in agricultural products. In this study, we added α-Fe2O3 nanoparticles (NPs) and biochar into Cd-contaminated soil to investigate physiological responses of muskmelon in the whole life cycle. RESULTS: The results showed that Cd caused adverse impacts on muskmelon (Cucumis melo) plants. For instance, the chlorophyll of muskmelon leaves in the Cd alone treatment was reduced by 8.07-32.34% in the four periods, relative to the control. The treatments with single amendment, α-Fe2O3 NPs or 1% biochar or 5% biochar, significantly reduced the soil available Cd content, but the co-exposure treatments (α-Fe2O3 NPs and biochar) had no impact on the soil available Cd content. All treatments could reduce the Cd content by 47.64-74.60% and increase the Fe content by 15.15-95.27% in fruits as compared to the Cd alone treatment. The KEGG enrichment results of different genes in different treatments indicated that single treatments could regulate genes related to anthocyanin biosynthesis, glutathione metabolism and MAPK signal transduction pathways to reduce the Cd toxicity. CONCLUSIONS: Overall the combination of biochar and α-Fe2O3 NPs can alleviate Cd toxicity in muskmelon. The present study could provide new insights into Cd remediation in soil using α-Fe2O3 NPs and biochar as amendments.

A Multilevel Study of Melon Fruit Reticulation Provides Insight into Skin Ligno-Suberization Hallmarks.

The skin of fleshy fruit is typically covered by a thick cuticle. Some fruit species develop different forms of layers directly above their skin. Reticulation, for example, is a specialized suberin-based coating that ornaments some commercially important melon (Cucumis melo) fruit and is an important quality trait. Despite its importance, the structural, molecular, and biochemical features associated with reticulation are not fully understood. Here, we performed a multilevel investigation of structural attributes, chemical composition, and gene expression profiles on a set of reticulated and smooth skin melons. High-resolution microscopy, surface profiling, and histochemical staining assays show that reticulation comprises cells with heavily suberized walls accumulating large amounts of typical suberin monomers, as well as lignified cells localized underneath the specialized suberized cell layer. Reticulated skin was characterized by induced expression of biosynthetic genes acting in the core phenylpropanoid, suberin, lignin, and lignan pathways. Transcripts of genes associated with lipid polymer assembly, cell wall organization, and loosening were highly enriched in reticulated skin tissue. These signatures were exclusive to reticulated structures and absent in both the smooth surfaces observed in between reticulated regions and in the skin of smooth fruit. Our data provide important insights into the molecular and metabolic bases of reticulation and its tight association with skin ligno-suberization during melon fruit development. Moreover, these insights are likely to contribute to melon breeding programs aimed at improving postharvest qualities associated with fleshy fruit surface layers.

Allopolyploidization in Cucumis contributes to delayed leaf maturation with repression of redundant homoeologous genes.

The important role of polyploidy in plant evolution is widely recognized. However, many questions remain to be explored to address how polyploidy affects the phenotype of the plant. To shed light on the phenotypic and molecular impacts of allopolyploidy, we investigated the leaf development of a synthesized allotetraploid (Cucumis × hytivus), with an emphasis on chlorophyll development. Delayed leaf maturation was identified in C. × hytivus, based on delayed leaf expansion, initial chlorophyll deficiency in the leaves and disordered sink-source transition. Anatomical observations also revealed disturbed chloroplast development in C. ×hytivus. The determination of chlorophyll biosynthesis intermediates suggested that the chlorophyll biosynthesis pathway of C. × hytivus is blocked at the site at which uroporphyrinogen III is catalysed to coproporphyrinogen III. Three chlorophyll biosynthesis-related genes, HEMA1, HEME2 and POR, were significantly repressed in C. × hytivus. Sequence alignment showed both synonymous and non-synonymous substitutions in the HEMA1, HEME2 and POR genes of the parents. Cloning of the chlorophyll biosynthetic genes suggested the retention of homoeologs. In addition, a chimeric clone of the HEMA1 gene that consisted of homologous genes from the parents was identified in C. × hytivus. Overall, our results showed that allopolyploidization in Cucumis has resulted in disturbed chloroplast development and reduced chlorophyll biosynthesis caused by the repressed expression of duplicated homologous genes, which further led to delayed leaf maturation in the allotetraploid, C. × hytivus. The preferential retention/loss of certain types of genes and non-reciprocal homoeologous recombination were also supported in the present study, which provides new insights into the impact of allopolyploidy.

Hiseq Base Molecular Characterization of Soil Microbial Community, Diversity Structure, and Predictive Functional Profiling in Continuous Cucumber Planted Soil Affected by Diverse Cropping Systems in an Intensive Greenhouse Region of Northern China.

Cover crops are key determinants of the ecological stability and sustainability of continuous cropping soils. However, their agro-ecological role in differentially reshaping the microbiome structure and functioning under a degraded agroecosystem remains poorly investigated. Therefore, structural and metabolic changes in soil bacterial community composition in response to diverse plant species were assessed. Winter catch leafy vegetables crops were introduced as cover plants in a cucumber-fallow period. The results indicate that cover crop diversification promoted beneficial changes in soil chemical and biological attributes, which increased crop yields in a cucumber double-cropping system. Illumina high-throughput sequencing of 16S rRNA genes indicated that the bacterial community composition and diversity changed through changes in the soil properties. Principal component analysis (PCA) coupled with non-metric multidimensional scaling (NMDS) analysis reveals that the cover planting shaped the soil microbiome more than the fallow planting (FC). Among different cropping systems, spinach-cucumber (SC) and non-heading Chinese cabbage-cucumber (NCCC) planting systems greatly induced higher soil nutrient function, biological activity, and bacterial diversity, thus resulting in higher cucumber yield. Quantitative analysis of linear discriminant analysis effect size (LEfSe) indicated that Proteobacteria, Actinobacteria, Bacteroidetes, and Acidobacteria were the potentially functional and active soil microbial taxa. Rhizospheres of NCCC, leaf lettuce-cucumber (LLC), coriander-cucumber (CC), and SC planting systems created hotspots for metabolic capabilities of abundant functional genes, compared to FC. In addition, the predictive metabolic characteristics (metabolism and detoxification) associated with host-plant symbiosis could be an important ecological signal that provides direct evidence of mediation of soil structure stability. Interestingly, the plant density of non-heading Chinese cabbage and spinach species was capable of reducing the adverse effect of arsenic (As) accumulation by increasing the function of the arsenate reductase pathway. Redundancy analysis (RDA) indicated that the relative abundance of the core microbiome can be directly and indirectly influenced by certain environmental determinants. These short-term findings stress the importance of studying cover cropping systems as an efficient biological tool to protect the ecological environment. Therefore, we can speculate that leafy crop diversification is socially acceptable, economically justifiable, and ecologically adaptable to meet the urgent demand for intensive cropping systems to promote positive feedback between crop-soil sustainable intensification.

Identification of Chemotaxis Compounds in Root Exudates and Their Sensing Chemoreceptors in Plant-Growth-Promoting Rhizobacteria Bacillus amyloliquefaciens SQR9.

Chemotaxis-mediated response to root exudates, initiated by sensing-specific ligands through methyl-accepting chemotaxis proteins (MCP), is very important for root colonization and beneficial functions of plant-growth-promoting rhizobacteria (PGPR). Systematic identification of chemoattractants in complex root exudates and their sensing chemoreceptors in PGPR is helpful for enhancing their recruitment and colonization. In this study, 39 chemoattractants and 5 chemorepellents, including amino acids, organic acids, and sugars, were identified from 98 tested components of root exudates for the well-studied PGPR strain Bacillus amyloliquefaciens SQR9. Interestingly, mutant stain SQR9Δ8mcp, with all eight putative chemoreceptors completely deleted, lost the chemotactic responses to those 44 compounds. Gene complementation, chemotaxis assay, and isothermal titration calorimetry analysis revealed that McpA was mainly responsible for sensing organic acids and amino acids, while McpC was mostly for amino acids. These two chemoreceptors may play important roles in the rhizosphere chemotaxis of SQR9. In contrast, the B. amyloliquefaciens-unique chemoreceptor McpR was specifically responsible for arginine, and residues Tyr-78, Thr-131, and Asp-162 were critical for arginine binding. This study not only deepened our insights into PGPR-root interaction but also provided useful information to enhance the rhizosphere chemotaxis mobility and colonization of PGPR, which will promote their application in agricultural production.

Vermicomposting transforms allelopathic parthenium into a benign organic fertilizer.

Vermicompost, which had been derived solely by the action of the epigeic earthworm Eisenia fetida on parthenium (Parthenium hysterophorus), was tested for its impact on the germination and early growth of green gram (Vigna radiata), ladies finger (Abelmoschus esculentus) and cucumber (Cucumis sativus). Seedlings were germinated and grown in soil amended with 0 (control), 0.75, 1.5, 2, 4, 8, 20 and 40% (by weight) parthenium vermicompost. Even though parthenium is known to possess strong negative allelopathy, as also plant/animal toxicity in other forms, its vermicompost (VC) manifested none of these attributes. Rather the VC enhanced germination success, introduced plant-friendly physical features in the container media, increased biomass carbon, and was seen to promote early growth as reflected in several morphological and biochemical characteristics in plants which had received parthenium VC in comparison to those which had not. All these effects were statistically significant. Fourier Transform Infrared (FTIR) Spectrometry revealed that the phenols and the sesquiterpene lactones that are responsible for the negative allelopathic impact of parthenium were largely destroyed in the course of vermicomposting. FTIR spectra also indicated that lignin content of parthenium was reduced during its vermicomposting. The findings open up the possibility that several other invasives known for their negative allelopathy and toxicity may also produce vermicompost which may be plant-friendly and soil-friendly. It also makes it appear possible that the huge quantities of phytomass that is generated annually by parthenium can be gainfully utilized in producing organic fertilizer via vermicomposting, thereby providing a means of exercising some control over parthenium's rampant growth and invasion.

Effects of industrial wastewater on growth and biomass production in commonly grown vegetables.

In developing countries like Pakistan, irrigation of crops with industrial and municipal wastewater is a common practice. However, the impact of wastewater irrigation on vegetables growth has rarely been studied. Therefore, the present study was conducted to determine the effect of industrial wastewater on the germination and seedling growth of some commonly grown vegetables in Pakistan. Wastewater samples were collected from two different industries (marble industry and match alam factory) at Hayatabad Industrial Estate (HIE) in Peshawar, Pakistan, and their effect on different growth parameters of four vegetables including Hibiscus esculentus, Lactuca sativa, Cucumis sativus, and Cucumis melo was investigated. The obtained results revealed that wastewater from marble industry did not affect seed germination except a minor inhibition in H. esculentus. Effluents from match alam factory stimulated seed germination in C. melo and C. sativus but had no effect on seed germination in the other two vegetables. Wastewater increased root and shoot length in H. esculentus, L. sativa and C. melo, but decreased it in C. sativus. Similarly, differential effects of wastewater were observed on fresh and dry biomass of seedlings in all vegetables. It can be concluded that wastewater may have different effects on different crops, depending upon the nature of wastewater and sensitivity of a plant species to wastewater.

CYTOGENETICAL TREATISE OF INDIAN REPRESENTATIVE SPECIES OF CUCUMIS. A KARYOTYPIC APPROACH.

Karyomorphological studies have been carried out in nine species and five varieties of the genus Cucumis representing Indian gene pool. The present investigations reveal the occurrence of two somatic chromosome numbers 2n = 14, 24 in the genus. C. ritchiei and C. indicus the two new species, were found to be having somatic chromosome numbers of 2n = 24 and 2n = 20 respectively. The wild species viz. C. hystrix, C. setosus, C. prophetarum, C. dipsaceus, C. indicus have very less number of median-centromeric chromosomes, high asymmetry indices, while melon groups have intermediate number of median -centromeric chromosomes. C. sativus, C. callosus, C. ritchiei show lesser number median-cen-tromeric chromosomes and very less asymmetry indices. The importance of karyotypic variation with respect to speciation within the genus Cucumis have been discussed.

Modeling and inferring cleavage patterns in proliferating epithelia.

The regulation of cleavage plane orientation is one of the key mechanisms driving epithelial morphogenesis. Still, many aspects of the relationship between local cleavage patterns and tissue-level properties remain poorly understood. Here we develop a topological model that simulates the dynamics of a 2D proliferating epithelium from generation to generation, enabling the exploration of a wide variety of biologically plausible cleavage patterns. We investigate a spectrum of models that incorporate the spatial impact of neighboring cells and the temporal influence of parent cells on the choice of cleavage plane. Our findings show that cleavage patterns generate "signature" equilibrium distributions of polygonal cell shapes. These signatures enable the inference of local cleavage parameters such as neighbor impact, maternal influence, and division symmetry from global observations of the distribution of cell shape. Applying these insights to the proliferating epithelia of five diverse organisms, we find that strong division symmetry and moderate neighbor/maternal influence are required to reproduce the predominance of hexagonal cells and low variability in cell shape seen empirically. Furthermore, we present two distinct cleavage pattern models, one stochastic and one deterministic, that can reproduce the empirical distribution of cell shapes. Although the proliferating epithelia of the five diverse organisms show a highly conserved cell shape distribution, there are multiple plausible cleavage patterns that can generate this distribution, and experimental evidence suggests that indeed plants and fruitflies use distinct division mechanisms.

Detection of NEO in muskmelon fruits inoculated with Fusarium sulphureum and its control by postharvest ozone treatment.

Fusarium rot of muskmelon, caused by Fusarium spp., is one of the most important postharvest decays, that not only causes economic losses but leads to trichothecenes contamination. A rapid and sensitive method was developed for neosolaniol (NEO) analysis in muskmelon inoculated with F. sulphureum, utilizing acetonitrile/water (84:16, v/v) extraction and PriboFast M270 columns purification and UPLC-MS/MS detection. Method validation was evaluated by linearity (R ≥ 0.9990), recovery (88.1-136.9%), precision (RSD ≤ 3.97%) and sensitivity (LOD, 0.5 µg/kg; LOQ, 1.5 µg/kg). The effect of ozone treatment on Fusarium rot development and NEO accumulation in inoculated muskmelon was also evaluated. The results showed that UPLC-MS/MS method was suitable for analyzing NEO in inoculated muskmelon, and 1.10 mg/l ozone treatment for 120 min significantly controlled Fusarium rot development and NEO accumulation in fruits after 5, 8 and 11 days. In vivo tests showed that ozone at 1.10 mg/l effectively degraded NEO in acetonitrile.

Uptake and translocation of p,p'-dichlorodiphenyldichloroethylene supplied in hydroponics solution to Cucurbita.

Field studies show shoots of zucchini (Cucurbita pepo L.) accumulate various hydrophobic contaminants from soil, although many other plants do not, including cucumber (Cucumis sativus L.). To investigate the mechanism for this uptake, we presented p,p'-dichlorodiphenyldichloroethylene (DDE) to these two species in hydroponics solution. A mixture of DDE bound to Tenax beads stirred with a solution of water passing through a reservoir provided a flowing solution containing DDE at approximately 2 microg/L for many weeks duration. Approximately 90% of the DDE supplied in solution was adsorbed on the roots of both cucumber and zucchini. Less than 10% of the sorbed DDE was released subsequently when clean solution flowed past these contaminated roots for 9 d. The shoots of both species accumulated DDE, but the fraction that moved from the roots to the shoot in zucchini, ranging from 6 to 27% in various trials, was 10-fold greater than that in cucumber, 0.7 to 2%. The gradient in DDE concentration in zucchini tissues was in the order root more more than stem > petiole > leaf blade, indicating the movement was through the xylem in the transpiration stream. Some DDE in leaf blades might have been absorbed from the air, because the concentration in this tissue varied less with time, position in trough, or species, than did DDE in stems and petioles. The remarkable ability of zucchini to translocate DDE could not be attributed to differences in tissue composition, growth rate, distribution of weight among plant parts, or in the leaf area and rate of transpiration of water from leaves. Some other factor enables efficient translocation of hydrophobic organic contaminants in the xylem of zucchini.

Soil ecotoxicity assessment using cadmium sensitive plants.

Four crop plant species (sweet corn, Zea may; wheat, Triticum aestivum; cucumber, Cucumis sativus; and sorghum, Sorghum bicolor) were tested to assess an ecotoxicity in cadmium-amended soils. The measurement endpoints used were seed germination and seedling growth (shoot and root). The presence of cadmium decreased the seedling growth. The medium effective concentration values (EC50) for shoot or root growth were calculated by the Trimmed Spearman-Karber method. Due to the greater accumulation of Cd to the roots, root growth was a more sensitive endpoint than shoot growth. Bioavailability and transport of Cd within plant were related to concentration and species. The ratio of bioaccumulation factor (BAF) in the shoots to the roots indicated high immobilization of Cd in the roots. Seed germination was insensitive to Cd toxicity, and is not recommended for a suitable assay. Among the test plants and test endpoints, root growth of sorghum and cucumber appears to be a good protocol to assess ecotoxicity of soils contaminated by Cd.

Quantitative structure-activity relationships for the inhibition toxicity to root elongation of Cucumis sativus of selected phenols and interspecies correlation with Tetrahymena pyriformis.

The comparative toxicities of selected phenols to higher plants Cucumis sativus were measured and the negative logarithm molar concentration of the root elongation median inhibition (IRC50) were derived. Quantitative structure-activity relationships (QSARs) were developed to explore the toxicity influencing factors and for predictive purpose. The toxicity data, fell into two classes: polar narcosis and bio-reactive. For polar narcotic phenols, a highly significant two-parameter QSAR based on 1-octanol/water partition coefficient (logKow) and energy of the lowest unoccupied orbital (E(lumo)) was derived (IRC50 = 0.77 log Kow - 0.39E(lumo) + 2.36 n = 22 r2 = 0.89). The five bio-reactive chemicals proved to show elevated toxicity due to their typical substructure involved diverse reactive mechanisms. In an effort to model all chemicals, a robust multiple-variable QSAR combining logKow, E(lumo) and Qmax, the most negative net atomic charge, was developed (IRC50 = 0.65 logKow - 0.72E(lumo) + 0.23Qmax + 2.81 n = 27 r2 = 0.94), indicating that hydrophobicity, electrophilicity and hydrogen bond interaction contribute mainly to the phytotoxicity. The toxicological data was compared with Tetrahymena pyriformis 2-d population growth inhibition toxicity (IGC50) and excellent interspecies correlations were observed both for the polar narcotics and for five reactive chemicals (for polar narcotics: IRC50 = 0.95IGC50 + 1.07 n = 16 r2 = 0.89; for bio-reactive chemicals: IRC50 = 0.98IGC50 + 2.19 n = 5 r2 = 0.97; and for all: IRC50 = 0.93IGC50 + 1.63 n = 21 r2 = 0.87). This suggested that T pyriformis toxicity could serve as a surrogate of C. sativus toxicity for phenols and interspecies correlation also could be established for reactive chemicals.

Mechanism-based quantitative structure-activity relationships for the inhibition of substituted phenols on germination rate of Cucumis sativus.

Comparative inhibition activity (GC50) of 42 structurally diverse substituted phenols on seed germination rate of Cucumis sativus was investigated. Quantitative structure-activity relationships (QSARs) were developed by using hydrophobicity (1-octanol/water partition coefficient, logKow) and electrophilicity (the energy of the lowest unoccupied molecule orbital, Eluma) for the toxicity of phenols according to their modes of toxic action. Most phenols elicited their response via a polar narcotic mechanism and a highly significant log Kow-based model was obtained (GC50 = 0.92 log Kow + 1.99, r2 0.84, n = 29). The inclusion of E(lumo) greatly improved the predictive power of the polar narcotic QSAR (GC50 = 0.88 log Kow - 0.30E(lumo) + 1.99, r2 = 0.93, n = 29). pKa proved to be an insignificant influencing factor in this study. Poor correlation with hydrophobicity and strong correlation with electrophilicity were observed for the nine bio-reactive chemicals. Their elevated toxicity was considerably underestimated by the polar narcotic logKow-dependent QSAR. The nine chemicals consist of selected nitro-substituted phenols, hydroquinone, catechol and 2-aminophenol. Their excess toxic potency could be explained by their molecular structure involving in vivo reaction with bio-macromolecules. Strong dissociation of carboxyl group of the four benzoic acid derivatives greatly decreased their observed toxicity. In an effort to model all chemicals including polar narcotics and bio-reactive chemicals, a response-surface analysis with the toxicity, logKow and E(lumo) was performed. This resulted in a highly predictive two-parameter QSAR for most of the chemicals (GC50 = 0. 70 logKow - 0.66E(lumo) + 2.17, r2 = 0.89, n = 36). Catechol and 2,4-dinitrophenol proved to be outliers of this model and their much high toxicity was explained.

The effects of sewage sludge and sewage sludge biochar on PAHs and potentially toxic element bioaccumulation in Cucumis sativa L.

The presence of contaminants such as polycyclic aromatic hydrocarbons (PAHs) and potentially toxic elements (PTEs), including As, Cd, Cu, Pb and Zn, restricts the application of sewage sludge (SS) to agricultural land. This research established that the conversion of SS to SS biochar (SSBC) significantly (p ≤ 0.01) decreased PAH and available PTE concentrations. Once added to soil both SS and SSBC significantly (p ≤ 0.05) decrease PAH availability. Bioaccumulation of PAHs into Cucumis sativa L. was reduced by both SSBC (44-57%) and (to a lesser extent 20-36%) by SS. Following addition to soil SSBC significantly (p ≤ 0.05) reduced available PTEs (except Cd), while SS significantly (p ≤ 0.05) increased PTE availability. As a consequence SSBC significantly (p ≤ 0.05) reduced PTE bioaccumulation (except Cd and Zn), while SS increased PTE bioaccumulation. These results suggest SSBC to be a candidate for soil amendment that offers advantages over SS in terms of PAH/PTE bioaccumulation mitigation.

The role of abscisic acid in regulating cucumber fruit development and ripening and its transcriptional regulation.

Cucumber (Cucumis sativus L.), a kind of fruit usually harvested at the immature green stage, belongs to non-climacteric fruit. To investigate the contribution of abscisic acid (ABA) to cucumber fruit development and ripening, variation in ABA level was investigated and a peak in ABA level was found in pulp before fruit get fully ripe. To clarify this point further, exogenous ABA was applied to cucumber fruits at two different development stages. Results showed that ABA application at the turning stage promotes cucumber fruit ripening, while application at the immature green stage had inconspicuous effects. In addition, with the purpose of understanding the transcriptional regulation of ABA, two partial cDNAs of CsNCED1 and CsNCED2 encoding 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in ABA biosynthetic pathway; one partial cDNA of CsCYP707A1 for 8'-hydroxylase, a key enzyme in the oxidative catabolism of ABA and two partial cDNAs of CsBG1 and CsBG2 for ß-glucosidase (BG) that hydrolyzes ABA glucose ester (ABA-GE) to release active ABA were cloned from cucumber. The DNA and deduced amino acid sequences of these obtained genes respectively showed high similarities to their homologous genes in other plants. Real-time PCR analysis revealed that ABA content may be regulated by its biosynthesis (CsNCEDs), catabolism (CsCYP707A1) and reactivation genes (CsBGs) at the transcriptional level during cucumber fruit development and ripening, in response to ABA application, dehydration and pollination, among which CsNCED1, CsCYP707A1 and CsBG1 were highly expressed in pulp and may play more important roles in regulating ABA metabolism.

Effect of aeration rate, C/N ratio and moisture content on the stability and maturity of compost.

To estimate the order of importance of factors affecting the stability and maturation of compost, pig feces and corn stalks were co-composted at different aeration rates (AR: 0.24, 0.48, 0.72 L kg(-1)dry matter (DM)min(-1)), C/N ratios (15, 18, 21), and moisture contents (MC: 65%, 70%, 75%). The thermophilic phase with all treatments was long enough to meet sanitation requirements. The oxygen content and N losses increased with increasing AR, but no significant differences were observed between the moderate and high treatments. The compost with the lowest initial C/N ratio was significantly different from the other treatments and had the lowest germination index (53-66%). AR was the main factor influencing compost stability, while the C/N ratio mainly contributed to compost maturity, and the MC had an insignificant effect on the compost quality. The recommended parameters for composting are an AR of 0.48 L kg(-1) DM min(-1) and a C/N ratio of 18 with MCs of 65-75%.

[Sex determination in cucurbits]. / Le déterminisme du sexe chez les cucurbitacées.

Sex determination in plants leads to the development of unisexual flowers from an originally bisexual floral meristem. Cucurbits are not only species of agronomic interest but they also represent model species for the study of plant sex determination, because of their ability to harbor different sexual types. Such sexual forms are controlled by the identity of the alleles at the following loci: andromonoecious (a) and gynoecious (g) in melon, or androecious (a), Female (F), and Monoecious (M) in cucumber. We firstly showed that the andromonoecious a gene in melon encodes for an ACC synthase (CmACS7) and demonstrated that andromonoecy results from a mutation in the active site of the enzyme. Expression of the active enzyme inhibits the development of the male organs and is not required for carpel development. Because the a gene in melon and M gene in cucumber control the same sexual transition, monoecy to andromonoecy, we isolated the andromonoecy M gene in cucumber using a candidate gene approach in combination with genetic and biochemical analysis. We demonstrated the co-segregation of CsACS2, a close ortholog of CmACS7, with the M locus, and showed that the cucumber andromonoecious phenotype is also due to a loss of ACS enzymatic activity. CsACS2 is expressed specifically in carpel primordia of female flowers and should play a similar role to that of CmACS7 in melon in the inhibition of stamina development. Finally, we also showed that the transition from male to female flowers in the gynoecious lines results from epigenetic changes in the promoter of a C(2)H (2) zinc-finger transcription factor, CmWIP1. This epigenetic change is elicited by the insertion of a DNA transposon, which causes the spreading of DNA methylation to the CmWIP1 promoter. Expression of CmWIP1 leads to carpel abortion, resulting in the development of unisexual male flowers. From all these results, we built a model in which CmACS7 and CmWIP1 interact to control the development of male, female and hermaphrodite flowers in melon.

Evaluation of the combination of 1,3-dichloropropene and dazomet as an efficient alternative to methyl bromide for cucumber production in China.

BACKGROUND: The combination of 1,3-dichloropropene (1,3-D) and dazomet (DZ) offers a potential alternative to methyl bromide (MB) for soil disinfection. MB is scheduled to be withdrawn from routine use by 2015 in developing countries. Combination treatments of 1,3-D + DZ were evaluated in a laboratory study and in two commercial cucumber fields. RESULTS: Laboratory studies found that nearly all of the tested combinations of 1,3-D and DZ displayed positive synergistic activity on root-knot nematodes (Meloidogyne spp.), two major soilborne fungi (Fusarium spp. and Phytophthora spp.) and the seeds of two major weed species (Digitaria sanguinalis and Abutilon theophrasti). Field trials revealed that the combination of 1,3-D and DZ (at 10 + 25 g m(-2) ) successfully suppressed Meloidogyne spp. root galling, sharply reduced Fusarium spp. and Phytophthora spp. and maintained high cucumber yields. The combination treatment of 1,3-D + DZ was more effective than 1,3-D or DZ alone and provided results similar to methyl bromide with respect to pest control, plant mortality, plant height, yield and income. All of the treatments were significantly better than the non-treated control. CONCLUSION: The results indicate that the tested combination of 1,3-D and DZ offers an efficient alternative to methyl bromide for cucumber production.

Phenolic compound production and biological activities from in vitro regenerated plants of gherkin (Cucumis anguria L. )

Background The effect of polyamines (PAs) along with cytokinins (TDZ and BAP) and auxin (IBA) was induced by the multiple shoot regeneration from leaf explants of gherkin (Cucumis anguria L.). The polyphenolic content, antioxidant and antibacterial potential were studied from in vitro regenerated and in vivo plants. Results Murashige and Skoog (MS) medium supplemented with 3% sucrose containing a combination of 3.0 µM TDZ, 1.0 µM IBA and 75 µM spermidine induced maximum number of shoots (45 shoots per explant) was achieved. Regenerated shoots elongated in shoot elongation medium containing 1.5 µM GA3 and 50 µM spermine. The well-developed shoots were transferred to root induction medium containing 1.0 µM IBA and 50 µM putrescine. Rooted plants were hardened and successfully established in soil with a 95% survival rate. Twenty-five phenolic compounds were identified by ultra-performance liquid chromatography (UPLC) analysis The individual polyphenolic compounds, total phenolic and flavonoid contents, antioxidant and antibacterial potential were significantly higher with in vitro regenerated plants than in vivo plants. Conclusions Plant growth regulators (PGRs) and PAs had a significant effect on in vitro plant regeneration and also a biochemical accumulation of flavonols, hydroxybenzoic and hydroxycinnamic acid derivatives in C. anguria. Due to these metabolic variations, the antioxidant and antibacterial activities were increased with in vitro regenerated plants than in vivo plants. This is the first report describing the production of phenolic compounds and biological activities from in vitro and in vivo regenerated plants of C. anguria.