RESUMO
Most government published statistics of C. bovis prevalence in Botswana emanate mainly from records available at the Botswana Meat Commission (BMC), the country's national export abattoir. Although BMC slaughters 44% of Botswana's annual cattle slaughter, prevalence data arising from BMC does not reflect prevalence from lower throughput abattoirs and potential hotspots. Thus, reporting national prevalence rate using solely BMC statistics may not be very informative and reflective of the bigger picture. It therefore became imperative to probe prevalence of bovine cysticercosis using a cross-sectional study through passive abattoir inspection, covering a wider scope (more regions) and some lower throughput abattoirs previously not accounted for. Furthermore, non-participatory interview using structured questionnaires was employed to actively elicit prevalence information directly from meat industry stake holders. Prevalence arising from survey was used to compare and query results from statutory (traditional) passive abattoir method. Abattoir prevalence was 17.17% (SE = 1.70027), and survey prevalence was 42.35%; both of which were higher than published prevalence of 13.5% and BMC prevalence of 10% (SE = 0.006576). Survey method was more holistic than passive abattoir method, by covering more frontiers thus yielding higher prevalence. At p = 025, abattoir and survey prevalence were significantly different from each other. In addition to delimitating novel hotspots in Botswana, this study showed significant difference, p = 0.002 in prevalence within districts and regions. Kalagadi district's prevalence differed significantly from other districts: differed from North East at p = 0.042, Central district at p = 0.002 and Ghanzi at p = 0.004. The results which arise from this methodological approach have been able to provide a more all-inclusive and reliable prevalence rate.
Assuntos
Matadouros , Autopsia/veterinária , Doenças dos Bovinos/epidemiologia , Cisticercose/veterinária , Cysticercus/isolamento & purificação , Animais , Autopsia/estatística & dados numéricos , Botsuana/epidemiologia , Bovinos , Doenças dos Bovinos/parasitologia , Cisticercose/epidemiologia , Cisticercose/parasitologia , Prevalência , Inquéritos e QuestionáriosRESUMO
We report the complete coding sequences of mitochondrial thioredoxin (TsTrx2) and glutaredoxin (TsGrx1) from the cysticerci of T. solium. The full-length DNA of the TsTrx2 gene shows two introns of 88 and 77 bp and three exons. The TsTrx2 gene contains a single ORF of 423 bp, encoding 140 amino acid residues with an estimated molecular weight of 15,560 Da. A conserved C64NPC67 active site and a 30-amino acid extension at its N-terminus were identified. An insulin reduction reaction was used to determine whether it was a functional recombinant protein. The full-length DNA of the TsGrx1 gene shows one intron of 39 bp and a single ORF of 315 bp, encoding 105 amino acid residues with an estimated molecular weight of 12,582 Da. Sequence analysis revealed a conserved dithiol C34PYC37 active site, GSH-binding motifs (CXXC, Lys and Gln/Arg, TVP, and CXD), and a conserved Gly-Gly motif. The r-TsGrx1 kinetic constants for glutathione (GSH) and 2-hydroxyethyl disulfide (HED) were determined. In addition, cytosolic thioredoxin (TsTrx1), as reported by (Jiménez et al., Biomed Res Int 2015:453469, 2015), was cloned and expressed, and its catalytic constants were obtained along with those of the other two reductases. Rabbit-specific antibodies showed immune cross-reactions between TsTrx1 and TsTrx2 but not with TsGrx1. Both TsTGRs as reported by (Plancarte and Nava, Exp Parasitol 149:65-73, 2015) were biochemically purified to obtain and compare the catalytic constants for their natural substrates, r-TsTrx1, and r-TsTrx2, compared to those for Trx-S2E. coli. In addition, we determined the catalytic differences between the glutaredoxin activity of the TsTGRs compared with r-TsGrx1. These data increase the knowledge of the thioredoxin and GSH systems in T. solium, which is relevant for detoxification and immune evasion.
Assuntos
Citosol/metabolismo , Glutarredoxinas/genética , Glutarredoxinas/isolamento & purificação , Mitocôndrias/metabolismo , Taenia solium/genética , Tiorredoxinas/genética , Tiorredoxinas/isolamento & purificação , Sequência de Aminoácidos , Animais , Clonagem Molecular , Cysticercus/genética , Cysticercus/isolamento & purificação , Cysticercus/metabolismo , Citosol/química , Dissulfetos/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Etanol/análogos & derivados , Etanol/metabolismo , Glutarredoxinas/química , Glutarredoxinas/metabolismo , Glutationa/metabolismo , Cinética , Mitocôndrias/química , Mitocôndrias/genética , Fases de Leitura Aberta , Coelhos , Taenia solium/metabolismo , Tiorredoxinas/química , Tiorredoxinas/metabolismoRESUMO
Neurocysticercosis accounts for approximately 30% of all epilepsy cases in most developing countries. The immunodiagnosis of cysticercosis is complex and strongly influenced by the course of infection, the disease burden, the cyst location, and the immune response of the host. The main approach to immunodiagnosis should thus be to evaluate whether the serological results are consistent with the diagnosis suggested by imaging. Antibody detection is performed using lentil lectin-purified parasite antigens in an enzyme-linked immunoelectrotransfer blot format, while antigen detection uses a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA). Promising new assay configurations have been developed for the detection of both antibody and antigen, including assays based on synthetic or recombinant antigens that may reduce costs and improve assay reproducibility and multiplex bead-based assays that may provide simultaneous quantitative results for several target antigens or antibodies.
Assuntos
Cysticercus/imunologia , Imunoensaio , Testes Imunológicos , Neurocisticercose/diagnóstico , Taenia solium/imunologia , Animais , Anticorpos Anti-Helmínticos/sangue , Anticorpos Anti-Helmínticos/líquido cefalorraquidiano , Anticorpos Anti-Helmínticos/imunologia , Antígenos de Helmintos/sangue , Antígenos de Helmintos/imunologia , Cysticercus/isolamento & purificação , Humanos , Neurocisticercose/parasitologia , Neurocisticercose/patologia , Reprodutibilidade dos Testes , Taenia solium/isolamento & purificaçãoRESUMO
BACKGROUND: Vietnam is endemic for taeniasis and T. solium cysticercosis. Despite this, information on the epidemiological characteristics of the diseases in the Central Highlands of Vietnam are poorly described. The aims of this study were to determine the epidemiological characteristics of taeniasis (Taenia spp.) and T. solium cysticerci exposure in humans in Dak Lak province in the Central Highlands, Vietnam. METHODS: This cross-sectional study was carried out in six villages in three districts of Dak Lak. A total of 190 households were visited. From each household, between one and five individuals were asked to donate a single faecal and blood sample and respond to a questionnaire. Serum samples were subjected to lentil lectin purified glycoprotein enzyme-linked immunoelectrotransfer blot assay to detect antibodies against T. solium cysticerci. Multiplex real-time PCR was used to detect Taenia spp. infection in faecal samples. A fixed-effects logistic regression model was developed to identify factors associated with the probability of Taenia spp. infection or T. solium cysticerci exposure risk. The contribution of each of identified factor was quantified using population attributable fractions. RESULTS: The prevalence of seroexposure to T. solium in Dak Lak was 5% (95% CI 3% to 8%). Consumption of raw vegetables, sourcing drinking water from lakes, streams or ponds and the practice of outdoor defaecation were identified as primary risk factors for the prevalence of T. solium cysticerci exposure, while consuming undercooked pork and beef, pork tongue and observing Taenia proglottids in stool were associated with Taenia spp. infection. Consumption of raw vegetables attributed to 74% of T. solium cysticerci exposure-positive cases and consumption of undercooked beef attributed to 77% of taeniasis cases in these communities. CONCLUSIONS: The prevalence of T. solium seroexposure in Dak Lak is consistent with those reported in other regions of Vietnam. The identified risk factors associated with the prevalence of T. solium seroexposure and taeniasis infection in Dak Lak are modifiable and thus advocate for targeted community intervention programs to mitigating these risks.
Assuntos
Cisticercose/diagnóstico , Taenia solium/isolamento & purificação , Teníase/diagnóstico , Adolescente , Adulto , Animais , Anticorpos Antiprotozoários/sangue , Estudos Transversais , Cisticercose/epidemiologia , Cisticercose/parasitologia , Cysticercus/genética , Cysticercus/isolamento & purificação , DNA de Protozoário/isolamento & purificação , DNA de Protozoário/metabolismo , Fezes/parasitologia , Feminino , Humanos , Masculino , Carne/parasitologia , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Inquéritos e Questionários , Taenia solium/genética , Taenia solium/crescimento & desenvolvimento , Teníase/epidemiologia , Teníase/parasitologia , Vietnã/epidemiologiaRESUMO
A captive-born adult female Nilgiri langur ( Semnopithecus johnii) developed an edematous swelling of the left thigh and a firm mass around the right ankle joint. The animal also suffered from lethargy and anorexia and was euthanized because of poor general condition. Necropsy revealed that the skeletal muscle of the left thigh had been replaced by a multilocular cystic mass containing numerous sand-grain-sized whitish structures. Small cysts were also present in the lung and the myocardium. The mass of the right ankle joint was histologically consistent with a myxosarcoma. In contrast, the cystic masses from the left thigh, the lung, and the myocardium represented metacestode tissue with evidence of numerous larval cestodes consistent with cysticerci. Cysticerci showed morphological characteristics of Cysticercus longicollis, the larval form of Taenia crassiceps, which was confirmed by genetic analysis. This is the first documented case of a Taenia crassiceps cysticercosis in an Old World monkey species.
Assuntos
Colobinae , Cisticercose/veterinária , Cysticercus/isolamento & purificação , Doenças dos Macacos/diagnóstico , Animais , Animais de Zoológico , Cisticercose/diagnóstico , Cisticercose/parasitologia , Feminino , Alemanha , Doenças dos Macacos/parasitologiaRESUMO
Taeniasis/cysticercosis caused by the tapeworm Taenia solium is a parasite disease transmitted among humans and pigs, the main intermediate host. The larvae/cysts can lodge in several tissues of the pig, i.e. skeletal muscles and different locations of the central nervous system. The molecular mechanisms associated to tissue preferences of the cysts remain poorly understood. The major public health concern about this zoonosis is due to the human infections by the larval form in the central nervous system, causing a highly pleomorphic and debilitating disease known as neurocysticercosis. This study was aimed to explore the 2DE protein maps of T. solium cysts obtained from skeletal muscles and central nervous system of naturally infected pigs. The gel images were analyzed through a combination of PDQuest™ and multivariate analysis. Results showed that differences in the protein patterns of cysts obtained from both tissues were remarkably discrete. Only 7 protein spots were found specifically associated to the skeletal muscle localization of the cysts; none was found significantly associated to the central nervous system. The use of distinct protein fractions of cysts allowed preliminary identification of several tissue-specific antigenic bands. The implications of these findings are discussed, as well as several strategies directed to achieve the complete characterization of this parasite's proteome, in order to extend our understanding of the molecular mechanisms underlying tissue localization of the cysts and to open avenues for the development of immunological tissue-specific diagnosis of the disease.
Assuntos
Encéfalo/parasitologia , Cisticercose/veterinária , Cysticercus/química , Proteínas de Helminto/análise , Músculo Esquelético/parasitologia , Doenças dos Suínos/parasitologia , Taenia solium/química , Animais , Cisticercose/parasitologia , Cysticercus/isolamento & purificação , Eletroforese em Gel Bidimensional , Sus scrofa , Suínos , Taenia solium/isolamento & purificaçãoRESUMO
First case of Cysticercus longicollis, larval stage of Taenia crassiceps, was diagnosed in a wild adult male red fox (Vulpes vulpes). The fox was killed by dogs at Nature Park Medvednica and presented to the University of Zagreb Faculty of Veterinary Medicine with history of being unable to run away and having skin lesions on legs that resembled to those of mange. Necropsy revealed whitish fluctuant mass full of cysticercus-like structures, surrounded by fibrous capsule and placed between the leg muscles, and numerous of spherical cysts in the subcutis and in the peritoneal cavity. Cysticerci were identified as C. longicollis based on their size, number and size of the rostellar hooks, mode of proliferation and DNA analysis. To our knowledge, this is the first case of T. crassiceps cysticercosis in a wild carnivore.
Assuntos
Cisticercose/veterinária , Cysticercus/isolamento & purificação , Raposas , Animais , Cisticercose/patologia , MasculinoRESUMO
Taenia solium is the major cause of taeniasis and cysticercosis/neurocysticercosis (NCC) in the developing countries including India, but the existence of other Taenia species and genetic variation have not been studied in India. So, we studied the existence of different Taenia species, and sequence variation in Taenia isolates from human (proglottids and cysticerci) and swine (cysticerci) in North India. Amplification of cytochrome c oxidase subunit 1 gene (cox1) was done by polymerase chain reaction (PCR) followed by sequencing and phylogenetic analysis. We identified two species of Taenia i.e. T. solium and Taenia asiatica in our isolates. T. solium isolates showed similarity with Asian genotype and nucleotide variations from 0.25 to 1.01 %, whereas T. asiatica displayed nucleotide variations ranged from 0.25 to 0.5 %. These findings displayed the minimal genetic variations in North Indian isolates of T. solium and T. asiatica.
Assuntos
Cisticercose/parasitologia , Variação Genética , Neurocisticercose/parasitologia , Doenças dos Suínos/parasitologia , Taenia/genética , Teníase/parasitologia , Animais , Cisticercose/epidemiologia , Cysticercus/classificação , Cysticercus/genética , Cysticercus/isolamento & purificação , Genótipo , Humanos , Índia/epidemiologia , Neurocisticercose/epidemiologia , Filogenia , Suínos , Doenças dos Suínos/epidemiologia , Taenia/classificação , Taenia/isolamento & purificação , Taenia solium/classificação , Taenia solium/genética , Taenia solium/isolamento & purificação , Teníase/epidemiologiaRESUMO
Angistrongylus cantonensis is a zoonotic nematode parasite and causative agent of human angiostrongyliasis, which clinically presents as eosinophilic meningitis or meningoencephalitis. Diagnosis of the disease is problematic since parasitologic findings are infrequent, and infection determinations must be based on the clinical symptoms and serological tests with limited specificities and sensitivities. The aim of the present study was to identify and generate a novel recombinant protein from A. cantonensis and evaluate its efficacy in the diagnosis of human angiostrongyliasis when incorporated into a Western blot serodiagnostic system. A cDNA protein expression library from adult A. cantonensis was constructed, followed by immunoscreening with serum from confirmed infected patients to identify and isolate immunoreactive clones. One clone, designated fAC40, possessed a partial sequence encoding a LisH protein domain with a predicted molecular weight of 16 kDa and containing four predicted antigenic peptides. By incorporating recombinant fAC40 in Western immunoblot tests using a serum panel consisting of confirmed and clinically diagnosed cases of human angiostrongyliasis and other helminthic infections, fAC40 exhibited a sensitivity and specificity of 91.8 and 100 %, respectively, and a positive and negative predictive value of 100 and 97.19 %, respectively, in the diagnosis of angiostrongyliasis. Importantly, it was not reactive with antibodies from serum of patients infected with Gnathostoma spinigerum and Cysticercus cellulosae, infections that clinically present neurological symptoms similar to angiostrongyliasis. These data demonstrate that the 16-kDa recombinant protein from A. cantonensis possesses high potential as a candidate antigen for a more sensitive and specific serodiagnosis of human angiostrongyliasis.
Assuntos
Angiostrongylus cantonensis/imunologia , Antígenos de Helmintos/imunologia , Proteínas de Helminto/imunologia , Meningoencefalite/diagnóstico , Infecções por Strongylida/diagnóstico , Adulto , Sequência de Aminoácidos , Angiostrongylus cantonensis/genética , Angiostrongylus cantonensis/isolamento & purificação , Animais , Antígenos de Helmintos/genética , Sequência de Bases , Western Blotting , Cisticercose/diagnóstico , Cisticercose/parasitologia , Cysticercus/imunologia , Cysticercus/isolamento & purificação , Feminino , Gnathostoma/imunologia , Gnathostoma/isolamento & purificação , Gnatostomíase/diagnóstico , Gnatostomíase/parasitologia , Proteínas de Helminto/genética , Humanos , Immunoblotting , Meningoencefalite/parasitologia , Proteínas Recombinantes , Sensibilidade e Especificidade , Infecções por Strongylida/parasitologiaRESUMO
Cysticercosis, a parasitic disease caused by larval form of Taenia solium, is a major health concern in the developing world. The encysted larval stage can affect any part of the body, but are most frequently detected in brain, eye, skeletal muscle and subcutaneous tissue. Most muscular cysticercosis are almost always associated with central nervous system involvement or with multiple intramuscular cysts or both. Here we report an unusual case of cysticercosis in right rectus muscle which was an isolated muscle involvement without any other systemic manifestation.
Assuntos
Abdome/diagnóstico por imagem , Cisticercose/diagnóstico , Cysticercus/isolamento & purificação , Doenças Musculares/parasitologia , Reto do Abdome/parasitologia , Taenia solium , Adolescente , Animais , Feminino , Humanos , Imageamento por Ressonância Magnética , UltrassonografiaRESUMO
BACKGROUND: Human cystericosis is the infection caused by the larvae of pork tapeworm Taeniasolium. The infection commonly affects the muscle, the central nervous system and subcutaneous tissues. The involvement of the breast is unusual. OBJECTIVE: To present a 54 years old postmenopausal woman, a petty trader and a Jehovah witness who presented with a painless lump in the right breast which was increasing in size. The mass was clinically diagnosed by the Surgeon who examined her as a case of right breast cancer and an excisional biopsy was done. METHOD: A review of the case note, autopsy findings including gross and microscopic examinations and literature was done. RESULTS: A histopathological appraisal of biopsy revealed the presence of the typical cysticercus larva and a definitive diagnosis of right breast Cystericosis was made. The diagnosis of cysticercosis in unusual sites such as breast may be clinically difficult and this supports why all biopsies must be sent to the pathologists for definitive diagnosis. CONCLUSION: Human cystericosis of the breast is rare, nevertheless, this should be considered as a differential diagnosis for amass in the breast particularly in the tropics and developing countries. To the best of our knowledge, this is the first case from our centre.
Assuntos
Doenças Mamárias/patologia , Doenças Mamárias/parasitologia , Cisticercose/patologia , Cysticercus/isolamento & purificação , Granuloma de Corpo Estranho/patologia , Granuloma de Corpo Estranho/parasitologia , Animais , Neoplasias da Mama/diagnóstico , Diagnóstico Diferencial , Feminino , Humanos , Pessoa de Meia-Idade , NigériaRESUMO
BACKGROUND: The cestode Taenia hydatigena uses canids, primarily dogs, as definitive hosts, whereas the metacestode larval stage cysticercus infects a range of intermediate hosts, including domestic animals such as goats, sheep and pigs. OBJECTIVES: In this study, we aimed to determine the genetic differences and haplotypes of Cysticercus tenuicollis isolates obtained from sheep and goats slaughtered in the Bingol and Elazig provinces of Turkey. METHODS: C. tenuicollis isolates were collected from 44 sheep and 26 goats slaughtered in slaughterhouses in Bingol and Elazig. After the isolation of total genomic DNA from C. tenuicollis isolates, the genetic characterization of the partial mitochondrial cytochrome oxidase 1 (CO1) gene region (866 bp) was amplified using specific primers by polymerase chain reaction (PCR), the products were then sequenced, and haplotype and genetic diversity analyses were carried out. RESULTS: As a result of the haplotype network analyses, 34 different haplotypes were detected around the main haplotype (Hap02) arranged in a star-like configuration and separated from other haplotypes by 1-28 mutation steps and covering 22.85% (16/70) of all isolates. Twenty-seven polymorphic fields were detected, 77.77% (21/27) of which were parsimony-informative, and secondary haplotype and nucleotide diversity were observed. Additionally, we detected high intraspecies haplotype diversity (hd: 0.933) and high nucleotide diversity (π: 0.00383), with 27 different nucleotide variation positions among the haplotypes of the isolates. Tajima's D value was negative, indicating population expansion and/or selection purification. The significantly negative Fu's Fs values indicated recent population expansion or the presence of expected rare haplotypes. CONCLUSION: The results of this study confirmed that C. tenuicollis isolates clustered in one lineage and were closely related to the relevant reference sequences in different countries, confirming the circulation of C. tenuicollis in different geographical regions.
Assuntos
Cysticercus , Variação Genética , Doenças das Cabras , Cabras , Haplótipos , Doenças dos Ovinos , Animais , Turquia/epidemiologia , Ovinos , Doenças dos Ovinos/parasitologia , Doenças dos Ovinos/epidemiologia , Doenças das Cabras/parasitologia , Doenças das Cabras/epidemiologia , Cysticercus/genética , Cysticercus/isolamento & purificação , Cisticercose/veterinária , Cisticercose/parasitologia , Cisticercose/epidemiologia , Matadouros , Carneiro DomésticoRESUMO
Swine cysticercosis is very common in the developing countries where pigs are raised. Undercooked measly pork consumption leads to taeniasis; Taenia carriers act as source of human and swine cysticercosis and neurocysticercosis. Diagnosis of swine cysticercosis is important to break the cycle of disease transmission. The present study compared the neck muscle, tongue and eye examinations, and serum ELISA with different preparations (crude lysate, cyst fluid, scolex and cyst wall antigens) of Taenia solium cyst for the diagnosis of swine cysticercosis. Total of 24 pigs initially identified by neck muscle, tongue and eyelid examinations were purchased from local slaughter house and subjected to MRI for confirmation of cysticercosis. Sera from 20 MRI confirmed infected pigs and 50 disease free controls were subjected to ELISA with T. solium cyst antigens. Neck muscle examination was 100% sensitive and 75% specific for the diagnosis of swine cysticercosis, whereas tongue and eye examinations were 70% and 25% sensitive, respectively. ELISA with crude lysate had 85% sensitivity and 98% specificity. ELISA with cyst fluid, scolex and cyst wall antigens showed 70%, 65%, and 45% sensitivity, respectively. The present study showed that neck muscle examination was highly sensitive but less specific, while ELISA with crude antigens had reasonable sensitivity and high specificity for diagnosis of swine cysticercosis. ELISA with crude lysate can be used as a screening tool for swine infection.
Assuntos
Cisticercose/veterinária , Cysticercus/isolamento & purificação , Pálpebras/parasitologia , Músculos do Pescoço/parasitologia , Doenças dos Suínos/diagnóstico , Língua/parasitologia , Animais , Anticorpos Anti-Helmínticos/sangue , Área Sob a Curva , Cisticercose/diagnóstico , Cisticercose/epidemiologia , Cisticercose/parasitologia , Cysticercus/imunologia , Doenças Endêmicas/veterinária , Ensaio de Imunoadsorção Enzimática/veterinária , Índia/epidemiologia , Imageamento por Ressonância Magnética/veterinária , Sensibilidade e Especificidade , Suínos , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/parasitologia , Taenia solium/imunologia , Taenia solium/isolamento & purificaçãoRESUMO
The objective of this study is to compare the removal of Taenia eggs to the removal of Ascaris eggs in a wastewater stabilization pond system consisting of three ponds in series, where the hydraulic residence time distribution has been characterized via a tracer study supported by computational fluid dynamics modeling. Despite a theoretical hydraulic retention time of 30 days, the peak dye concentration was measured in the effluent of the first pond after only 26 hours. The smaller-sized Taenia eggs were detected in higher concentrations than Ascaris eggs in the raw wastewater. Ascaris eggs were not detected in the pond system effluent, but 45 Taenia eggs/L were detected in the system effluent. If some of these eggs were of the species Taenia solium, and if the treated wastewater were used for the irrigation of crops for human consumption, farmers and consumers could potentially be at risk for neurocysticercosis. Thus, limits for Taenia eggs in irrigation water should be established, and precautions should be taken in regions where pig taeniasis is endemic. The results of this study indicate that the theoretical hydraulic retention time (volume/flow) of a pond is not always a good surrogate for helminth egg removal.
Assuntos
Cisticercose/etiologia , Cysticercus/isolamento & purificação , Hidrodinâmica , Eliminação de Resíduos Líquidos/métodos , Águas Residuárias/parasitologia , Poluentes da Água/isolamento & purificação , Animais , Bolívia , Cisticercose/parasitologia , Cysticercus/patogenicidade , Monitoramento Ambiental , Humanos , Modelos Teóricos , Qualidade da Água/normasRESUMO
In a rodent (Rattus norvegicus) survey in Buenos Aires province, metacestodes of tapeworms were found encysted in the liver of the host. The aim of this work was the morphological and molecular identification of this parasite. To achieve the molecular characterization of the parasite, ribosomal (28S) and mitochondrial (COI) DNA were amplified and sequenced. Based on both morphological and molecular data using bioinformatic tools, the metacestode was identified as Cysticercus fasciolaris. The adult form of this tapeworm (Taenia taeniaeformis) commonly infects felid and canid mammalian hosts. This is the first report on the molecular identification of Cysticercus fasciolaris in Buenos Aires province (Argentina).
Assuntos
Cysticercus/anatomia & histologia , Cysticercus/genética , Ratos/parasitologia , Animais , Argentina , Cysticercus/classificação , Cysticercus/isolamento & purificaçãoRESUMO
Orbital myocysticercosis is a helminthic infestation with variable clinical presentations. Computed tomography is considered gold standard in orbital imaging. Evaluation of computed tomography films in out-patient departments (OPD) is important in the management of orbital diseases. In this case report, we highlight the use of a smart phone as an aid in OPD for studying computed tomography images in identifying a small cysticercus cyst of the superior rectus muscle, which was invisible on naked eye examination.
Assuntos
Telefone Celular , Cisticercose/diagnóstico por imagem , Cysticercus/isolamento & purificação , Infecções Oculares Parasitárias/diagnóstico por imagem , Doenças Orbitárias/diagnóstico por imagem , Animais , Humanos , Masculino , Tomografia Computadorizada por Raios X , Adulto JovemRESUMO
Infection of the larval form (cysticerco) of Taenia in any tissue or organ is known as the disease cysticercosis. Many sites of infection have been documented but the central nervous system has been the most common. It present a case report of a 19 years old patient with a subcutaneous cysticercosis confirmed with biopsy.
Assuntos
Doenças do Tecido Conjuntivo/parasitologia , Cisticercose , Cysticercus/isolamento & purificação , Tela Subcutânea/parasitologia , Animais , Biópsia , Doenças do Tecido Conjuntivo/patologia , Cisticercose/patologia , Feminino , Humanos , Adulto JovemRESUMO
To study diagnostic epitopes within the Taenia solium 8 kDa antigen family, six overlapping synthetic peptides from an 8 kDa family member (Ts8B2) were synthesized and evaluated by ELISA and MABA with sera from patients with neurocysticercosis (NCC), from infected pigs and from rabbits immunized with recombinant Ts8B2 protein. The pre-immune rabbit sera and the Ts8B2 recombinant protein served as negative and positive controls, respectively. A similar analysis was done with the already described antigenic peptides from another member of the 8 kDa family, highly similar to Ts8B2, the CyDA antigen. Surprisingly, neither the Ts8B2 peptides nor the CyDA peptides were recognized by infected human and porcine sera. However, the entire Ts8B2 recombinant, as well as amino and carboxy-terminal halves were recognized by the positive serum samples. The observed lack of recognition of linear Ts8B2 peptides suggests that the principal serological response to the Ts8B2 family is focused on conformational epitopes in contrast to the previously observed antigenicity of the CyDA peptides. This differential antigenicity of 8 kDa family peptides could be related with parasite antigenic variability. The fact that rabbits experimentally immunized with Ts8B2 did make anti-peptide antibodies to peptides Ts8B2-6 and CyDA-6, located in the carboxy-terminal region demonstrated that the Ts8B2 peptides are not intrinsically non-immunogenic.
Assuntos
Antígenos de Helmintos/imunologia , Cisticercose/diagnóstico , Epitopos/imunologia , Taenia solium/imunologia , Sequência de Aminoácidos , Animais , Variação Antigênica , Antígenos de Helmintos/química , Antígenos de Helmintos/genética , Clonagem Molecular , Cisticercose/imunologia , Cisticercose/parasitologia , Cysticercus/genética , Cysticercus/imunologia , Cysticercus/isolamento & purificação , Ensaio de Imunoadsorção Enzimática , Epitopos/química , Epitopos/genética , Regulação da Expressão Gênica , Humanos , Immunoblotting , Coelhos , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Sensibilidade e Especificidade , Alinhamento de Sequência , Suínos , Taenia solium/genética , Taenia solium/isolamento & purificaçãoRESUMO
Humans are the only known definitive host of the tapeworm Taenia solium and become a carrier by eating undercooked pork contaminated with "Cysticercus cellulosae" (cysticerci). Pigs act as an intermediate host and acquire cysticercosis by ingestion of eggs or proglottids from human feces, which develop into cysticerci within tissue, mostly without causing clinical symptoms in the host. Cysticercosis occurs in humans in a context of "fecal peril" by ingestion of egg-contaminated soil, water, vegetation, or auto-infestation. It has been reported in the published data that the separation of swine from humans, healthy cooking, and hygienic practices would lead to the eradication of the disease. However, cysticercosis is still a major public health problem in endemic regions, with more than 50 million infected people and is now a re-emerging disease in industrialized countries owing to human migration. It is the second most common cause of seizures in tropical countries. We report a case of oral cysticercosis in a 28-year-old woman who presented with a painless swelling in the ventral portion of the tongue. An excisional biopsy was performed, and histopathologic examination revealed a cystic cavity containing the tapeworm.
Assuntos
Cisticercose/diagnóstico , Doenças da Língua/parasitologia , Adulto , Albendazol/uso terapêutico , Animais , Anti-Helmínticos/uso terapêutico , Cisticercose/tratamento farmacológico , Cisticercose/parasitologia , Cysticercus/isolamento & purificação , Feminino , Humanos , Suínos , Doenças dos Suínos/prevenção & controle , Doenças da Língua/diagnóstico , Doenças da Língua/tratamento farmacológico , Vacinas/uso terapêutico , Zoonoses/parasitologiaRESUMO
Polymerase chain reaction (PCR) test was employed to detect Taenia solium DNA in muscle lesions for validation of the meat inspection results of slaughtered pigs. Two sets of oligonucleotide primers, one targeted against the large subunit rRNA gene (TBR primers) and the other targeted against cytochrome c oxidase subunit 1 gene (Cox1 primers) of T. solium were used in this study. On reactivity in PCR test, the TBR primers and the Cox1 primers yielded products of 286 and 984 bp, respectively, in cysticercosis positive cases. Both the sets of primers were found to be highly specific, since they did not yield any PCR product in negative controls. A total of 225 pig carcasses were screened for cysticercosis by meat inspection, out of which 25 carcasses with visible cysts (16 viable and 9 degenerated cysts) were also confirmed to be positive for cysticercosis in PCR test. However, out of the 35 carcasses with suspected lesions on meat inspection, only two were found to be positive for cysticercosis in PCR test. The detection limits for both the primer sets were analyzed. The TBR primer set could detect up to 10 pg of cysticercus DNA, whereas the Cox1 primer set could detect only up to 1 ng. It is evident from the study that PCR test is an efficient tool for validation of meat inspection results and also to rule out ambiguity in carcass judgment of suspected cases of porcine cysticercosis.