RESUMO
We investigated the transmission dynamics of a community of tick-borne pathogenic bacteria in a common European songbird (Parus major). Tick-naïve birds were infested with three successive batches (spaced 5 days apart) of field-collected Ixodes ricinus nymphs, carrying the following tick-borne bacteria: Rickettsia helvetica (16.9%), Borrelia garinii (1.9%), Borrelia miyamotoi (1.6%), Anaplasma phagocytophilum (1.2%) and Candidatus Neoehrlichia mikurensis (0.4%). Fed ticks were screened for the pathogens after moulting to the next developmental phase. We found evidence for early transmission (within 2.75 days after exposure) of R. helvetica and B. garinii, and to a lesser extent of A. phagocytophilum based on the increased infection rates of ticks during the first infestation. The proportion of ticks infected with R. helvetica remained constant over the three infestations. In contrast, the infection rate of B. garinii in the ticks increased over the three infestations, indicating a more gradual development of host tissue infection. No interactions were found among the different bacterium species during transmission. Birds did not transmit or amplify the other bacterial species. We show that individual birds can transmit several pathogenic bacterium species at the same time using different mechanisms, and that the transmission facilitation by birds increases the frequency of co-infections in ticks.
Assuntos
Doenças das Aves/transmissão , Ixodes/microbiologia , Aves Canoras/microbiologia , Doenças Transmitidas por Carrapatos/microbiologia , Doenças Transmitidas por Carrapatos/transmissão , Anaplasma/crescimento & desenvolvimento , Animais , Doenças das Aves/microbiologia , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Coinfecção , Rickettsia/crescimento & desenvolvimentoRESUMO
In the present study we analyzed stability of plasmid content in 34 Borrelia strains of three different species (13 Borrelia afzelii, 10 Borrelia garinii and 11 Borrelia burgodorferi sensu stricto) using pulse field gel electrophoresis (PFGE). During long-term in vitro cultivation consisting of 50 passages, plasmid loss was established in 46% of B. afzelii, 40% of B. garinii and 36% of B. burgdorferi sensu stricto strains. Loss of plasmids occurred as early as between the 5th and 10th passage, affected only plasmids in the range 9-41 kb but not plasmids in the range 50-68 kb and manifested with the loss of one to up to three plasmids.
Assuntos
Grupo Borrelia Burgdorferi/genética , Plasmídeos/genética , Grupo Borrelia Burgdorferi/classificação , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , DNA Bacteriano/genética , Eletroforese em Gel de Campo Pulsado , Genoma Bacteriano , Plasmídeos/análise , Inoculações Seriadas , Fatores de TempoRESUMO
We used polyclonal antisera and monoclonal antibodies (mAbs) to inhibit the growth of clonal populations of two strains of Borrelia burgdorferi, the Lyme disease agent, and thereby select for antibody-resistant mutants. mAbs were directed at the outer membrane proteins, OspA or OspB. Mutants resistant to the growth-inhibiting properties of the antibodies were present in the populations at frequencies ranging from 10(-5) to 10(-2). The several escape variants that were examined were of four classes. Class I mutants were resistant to all mAbs; they lacked OspA and OspB and the linear plasmid that encodes them. Two other proteins were expressed in larger amounts in class I mutants; mAbs to these proteins inhibited the mutant but not the wild-type cells. Class II mutants were resistant to some but not all mAbs; they had truncated OspA and/or OspB proteins. Class III mutants were resistant only to the selecting mAb; they had full-length Osp proteins that were not bound by the selecting antibody in Western blots. In two class III mutants resistant to different anti-OspA mAbs, missense mutations were demonstrated in the ospA genes. Class IV mutants were likewise resistant only to selecting antibody, but in this case the selecting antibody still bound in Western blots.
Assuntos
Anticorpos Antibacterianos/imunologia , Grupo Borrelia Burgdorferi/imunologia , Mutação , Sequência de Aminoácidos , Sequência de Bases , Southern Blotting , Western Blotting , Grupo Borrelia Burgdorferi/genética , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , DNA Bacteriano , Dados de Sequência Molecular , FenótipoRESUMO
Borrelia burgdorferi, the spirochetal agent of Lyme disease, is transmitted by Ixodes ticks. A vaccine based on B. burgdorferi outer surface protein (Osp) A protects mice from spirochete infection. Here we report on the expression of OspA on spirochetes inside engorging ticks and relate OspA expression to antispirochetal immunity. Spirochetes in the gut of unfed nymphal ticks were stained by an OspA antibody, whereas in feeding ticks, the majority of spirochetes in the gut and salivary glands did not stain with the antibody. Thus, OspA was not expressed on most spirochetes during transmission from the vector to the vertebrate host. To examine the mechanism of protection afforded by OspA antibody, mice were passively immunized with OspA antibody at different times relative to tick attachment. When OspA antibody was administered to mice before or at the time of tick attachment, spirochetal development events in the vector, such as growth and salivary gland invasion, were blocked and the mice were protected from B. burgdorferi infection. When OspA antibody was administered to mice 48 h after tick attachment, spirochetes persisted in the nymphs and the mice were not protected despite the presence of circulating antibodies in the host as well as in the tick blood meal. Thus, OspA immunity appears to be effective only during a narrow window time at the beginning of the blood meal when antibodies bind to OspA-expressing spirochetes in the tick gut and block transmission from the vector to the host.
Assuntos
Antígenos de Bactérias , Antígenos de Superfície/imunologia , Vetores Aracnídeos/microbiologia , Proteínas da Membrana Bacteriana Externa/imunologia , Vacinas Bacterianas/imunologia , Ixodes/microbiologia , Lipoproteínas , Doença de Lyme/transmissão , Animais , Anticorpos Antibacterianos/imunologia , Anticorpos Antibacterianos/uso terapêutico , Vetores Aracnídeos/imunologia , Vacinas Bacterianas/uso terapêutico , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Sistema Digestório/microbiologia , Imunofluorescência , Imunoterapia Adotiva , Ixodes/imunologia , Doença de Lyme/imunologia , Doença de Lyme/prevenção & controle , Camundongos , Camundongos Endogâmicos C3H , Glândulas Salivares/microbiologiaRESUMO
The aim of the present paper was to evaluate cyst formation and growth parameters of Borrelia garinii in a range of media differing in formulation and cost. A qualitative assessment of morphology and motility of B. garinii was conducted. All media were prepared aseptically and used in test tubes or Petri dishes. For each medium, the initial spirochete concentration was standardized to 10(3) spirochets/mL. The following culture media were suitable to grow B. garinii: Barbour-Stoenner-Kelly, brain heart infusion and PMR. Growth was minimal at six weeks post-inoculation and maximum spirochete density was observed between 9-12 weeks. Often, the cultures developed cysts of different sizes, isolated or in groups, with a spiraled portion of variable sizes, mainly in unfavorable culture media. Brazilian Lyme disease-like illness, also known as Baggio-Yoshinari syndrome (BYS), is a new and interesting emerging tick-borne disease, caused by Borrelia burgdorferi sensu lato spirochetes, only during its cystic forms. It has been assumed that the peculiar clinical and laboratory features of BYS are consequential to the absence of a human sucker Ixodes ricinus complex tick at risk areas in Brazil, supporting the concept that the borrelia phenotypic expression pattern is modified as it is transmitted through the host.
Assuntos
Grupo Borrelia Burgdorferi/efeitos dos fármacos , Meios de Cultura/farmacologia , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Meios de Cultura/química , Humanos , Fatores de TempoRESUMO
A fundamental tenet of microbial pathogenesis is that bacterial pathogens must overcome host iron limitation to establish a successful infection. Surprisingly, the Lyme disease pathogen Borrelia burgdorferi has bypassed this host defense by eliminating the need for iron. B. burgdorferi grew normally and did not alter gene expression in the presence of iron chelators. Furthermore, typical bacterial iron-containing proteins were not detected in cell lysates, nor were the genes encoding such proteins identified in the genome sequence. The intracellular concentration of iron in B. burgdorferi was estimated to be less than 10 atoms per cell, well below a physiologically relevant concentration.
Assuntos
Grupo Borrelia Burgdorferi/fisiologia , Ferro/metabolismo , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/genética , Grupo Borrelia Burgdorferi/genética , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Grupo Borrelia Burgdorferi/patogenicidade , Cálcio/metabolismo , Carbonil Cianeto m-Clorofenil Hidrazona/farmacologia , Quelantes/farmacologia , Meios de Cultura , Expressão Gênica , Genoma Bacteriano , Quelantes de Ferro/farmacologia , Magnésio/metabolismo , Manganês/metabolismo , Poliestirenos/farmacologia , Polivinil/farmacologia , Desacopladores/farmacologia , Zinco/metabolismoRESUMO
Borrelia burgdorferi, the agent of Lyme disease, is transmitted by ticks. During transmission from the tick to the host, spirochetes are delivered with tick saliva, which contains the salivary protein Salp15. Salp15 has been shown to protect spirochetes against B. burgdorferi-specific antibodies. We now show that Salp15 from both Ixodes ricinus and Ixodes scapularis protects serum-sensitive isolates of Borrelia burgdorferi sensu lato against complement-mediated killing. I. ricinus Salp15 showed strong protective effects compared to those of I. scapularis Salp15. Deposition of terminal C5b to C9 (one molecule each of C5b, C6, C7, and C8 and one or more molecules of C9) complement complexes, part of the membrane attack complex, on the surface of B. burgdorferi was inhibited in the presence of Salp15. In the presence of normal human serum, serum-sensitive Borrelia burgdorferi requires protection against complement-mediated killing, which is provided, at least in part, by the binding to the tick salivary protein Salp15.
Assuntos
Grupo Borrelia Burgdorferi/efeitos dos fármacos , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Ixodes/metabolismo , Proteínas e Peptídeos Salivares/farmacologia , Animais , Atividade Bactericida do Sangue , Grupo Borrelia Burgdorferi/imunologia , Grupo Borrelia Burgdorferi/metabolismo , Membrana Celular/metabolismo , Ativação do Complemento/imunologia , Complexo de Ataque à Membrana do Sistema Complemento/antagonistas & inibidores , Complexo de Ataque à Membrana do Sistema Complemento/metabolismo , Humanos , Ixodes/microbiologia , Proteínas e Peptídeos Salivares/genética , Proteínas e Peptídeos Salivares/metabolismoRESUMO
A suburban focus of Lyme borreliosis situated 11 km from the southern border of the city of Lublin (eastern Poland) was characterized. The focus covers an area of circa 100 km(2), surrounding 3 localities inhabited by circa 7,500 people engaged mostly in farming. It was demonstrated that on the area of focus the infection rate of Ixodes ricinus ticks with Borrelia burgdorferi, frequency of serological response of inhabitants to the antigen of Borrelia burgdorferi, and incidence of Lyme borreliosis were significantly (p<0.001) greater compared to the whole territory of Lublin province, and were respectively 13.1 % vs. 4.7 %, 33.0 % vs. 13.7 %, and 0.002 % vs. 0.00075 % .
Assuntos
Anticorpos Antibacterianos/sangue , Vetores Aracnídeos/microbiologia , Grupo Borrelia Burgdorferi/isolamento & purificação , Ixodes/microbiologia , Doença de Lyme/epidemiologia , Adulto , Animais , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Grupo Borrelia Burgdorferi/imunologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ninfa , Polônia/epidemiologia , Estudos SoroepidemiológicosRESUMO
A previously reported procedure for the introduction of Borrelia spirochetes into tick larvae by immersion in a suspension of spirochetes was tested on Ixodes ricinus (L.) ticks and three of the most medically important European Borrelia genomic species, B. burgdorferi sensu stricto, B. garinii and B. afzelii. The procedure was compared with "classical" infection of nymphs by feeding on infected mice. Both methods yielded comparable results (infection rate 44-65%) with the exception of B. afzelii, which produced better results using the immersion method (44%) compared with feeding on infected mice (16%). Nymphs infected by the immersion method at the larval stage were able to transmit the infection to naïve mice as shown by serology and PCR detection of spirochetal DNA in organs. The immersion method is faster than feeding on infected mice and provides more reproducible conditions for infection. It can be exploited for studies on both pathogen transmission and Borrelia-vector interactions.
Assuntos
Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Ixodes/microbiologia , Animais , Anticorpos Antibacterianos/sangue , DNA Bacteriano/genética , Ensaio de Imunoadsorção Enzimática , Feminino , Larva/microbiologia , Doença de Lyme/microbiologia , Doença de Lyme/transmissão , Camundongos , Ninfa/microbiologia , Reação em Cadeia da PolimeraseRESUMO
The developmental cycles of all B. burgdorferi s.l. genospecies present typical, main pattern described in the 90thies. The simple scheme might be modified according to the biology of species and hosts preference. Central European genospecies of B. burgdorferi s.l. can be associated with four groups of hosts playing the role of animal reservoirs. The group 1 contains genospecies associated with rodents as primary animal reservoir B. afzelii, B. garinii, B. burgdorferi sensu stricto, strains B. bavariensis (B. garinii OspA serotype 4). The group 2 involves B. valaisiana and most of B. garinii strains, associated with birds. The group 3 involves B. spielmanii, the reservoir hosts are Gliridae, and hedgehogs. The group 4 includes B. lusitaniae, the hosts are lizards. B. miyamotoi enzootic cycle seems to be similar to B. burgdorferi complex, however, differs by the transovarial transmission possibility. The divisions are not extreme; in the hosts group, infected with appropriate Borrelia genospecies, very often are found the specimens infected with other genospecies.
Assuntos
Grupo Borrelia Burgdorferi , Ixodes , Animais , Aves , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Europa (Continente) , Ouriços , LagartosRESUMO
In recent years, the number of Lyme disease or borreliosis cases in Eurasia has been dramatically increasing. This tick-borne disease is caused by Borrelia burgdorferi sensu lato, which includes B. burgdorferi sensu stricto, the main species found in North America, and B. afzelii and B. garinii, which are primarily responsible for the disease in Eurasia. Currently, research on Lyme disease has focused mainly on B. burgdorferi while B. afzelii and B. garinii, which cause disease with distinctly different symptoms, are less studied. The purpose of this study is to evaluate B. afzelii BO23 and B. garinii CIP 103362 as model organisms to study Eurasian Lyme disease. To begin our analyses, we sequenced, annotated the chromosomes of both species and compared them to B. burgdorferi strain B31. We also assayed shuttle vector, pBSV2, for transformation efficacy and demonstrated that these strains can be cultured on solid media. In addition, we characterized how physicochemical parameters (e.g., oxygen, osmolarity, oxidative stress) affect both growth and motility of the bacteria. Finally, we describe each strain's antibiotic susceptibility and accessed their ability to infect mice. In conclusion, B. afzelii BO23 was more practical for in vitro and in vivo studies than B. garinii CIP 103362.
Assuntos
Grupo Borrelia Burgdorferi/genética , Grupo Borrelia Burgdorferi/fisiologia , Animais , Antibacterianos/farmacologia , Grupo Borrelia Burgdorferi/efeitos dos fármacos , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Técnicas de Cultura de Células , Cromossomos Bacterianos , DNA Bacteriano , Genótipo , Gentamicinas/farmacologia , Doença de Lyme/microbiologia , Camundongos , Movimento , Concentração Osmolar , Estresse Oxidativo/fisiologia , Oxigênio/metabolismo , Fenótipo , Rifaximina/farmacologia , Transformação GenéticaRESUMO
The aim of this study was to investigate samples from dogs suggestive of active canine borreliosis (group A) by culture and PCR and the detection of antibodies against Borrelia burgdorferi sensu lato in order to confirm a presumptive clinical diagnosis of canine borreliosis by laboratory results. Criteria for such a diagnosis were: history of tick exposure, lameness, neurological signs, nephropathy, lethargy, anorexia, and fever. A total of 302 samples comprising EDTA blood, urine, synovial fluid, cerebrospinal fluid, and tissue (skin, synovial membrane, kidney) from 98 dogs (26 with arthritis, 46 with neurological signs, 21 with nephropathy, 5 with non-specific symptoms) were collected and examined. Moreover, 55 healthy dogs (group B) and 236 dogs with symptoms or injuries unlikely to be associated with borreliosis (group C) were included in this study. Blood serum samples collected from all individuals (n=389) were analysed by ELISA. Twenty-one (21%) out of 98 dogs from group A, 4 (7%) out of 55 from group B and 15 (6%) out of 236 dogs from group C were positive for antibodies against B. burgdorferi sensu lato. The seroprevalences between groups A, B and C differed significantly. None of the corresponding samples investigated by PCR and culture were positive for spirochetal DNA or viable spirochetes. Borrelia afzelii was grown from one EDTA-blood sample but the corresponding blood serum sample remained antibody-negative. Consequently, the etiologic role of B. afzelii in this case is unclear. In approximately 40% of the presumptive canine borreliosis cases, other lesions have been found to be responsible for clinical signs. This study affirms that a definitive diagnosis of canine borreliosis cannot be made by clinical symptoms and serology based on a single consultation. Moreover, this study clearly revealed that the diagnostic sensitivity is enhanced by a thorough consideration and exclusion of other diseases.
Assuntos
Grupo Borrelia Burgdorferi/imunologia , Doenças do Cão/diagnóstico , Doença de Lyme/veterinária , Animais , Antibacterianos/urina , Anticorpos Antibacterianos/sangue , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Doenças do Cão/epidemiologia , Cães , Feminino , Doença de Lyme/diagnóstico , Doença de Lyme/epidemiologia , Masculino , Reação em Cadeia da Polimerase , Suíça/epidemiologia , Urina/químicaRESUMO
The biological diagnosis of Borrelia burgdorferi sensu lato infection is usually made by antibody detection in patient sera. Thus, serological testing (Elisa, immunoblotting) is essential for a biological diagnosis. Specific antibody detection is usually done in serum and CSF of patients suspected of Lyme borreliosis. Laboratories must follow European recommendations to validate these assays in routine practice. Antibody detection lacks sensitivity in the early cutaneous phase of the infection. Therefore, serological testing is not recommended for the diagnosis of erythema migrans. The interpretation of serology must take into account the variability of Elisa sensitivity and specificity and the lack of standardization for Western-blotting in Europe. Besides these indirect diagnosis techniques, there is also direct detection of spirochetes by culture or by in vitro DNA amplification but these require adequate samples. These molecular tests must not be performed routinely, but only for specific clinical situations and in specialized laboratories only.
Assuntos
Doença de Lyme/diagnóstico , Antígenos de Superfície/análise , Antígenos de Superfície/genética , Artrite/microbiologia , Proteínas da Membrana Bacteriana Externa/análise , Proteínas da Membrana Bacteriana Externa/genética , Vacinas Bacterianas/análise , Vacinas Bacterianas/genética , Grupo Borrelia Burgdorferi/genética , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Grupo Borrelia Burgdorferi/isolamento & purificação , Humanos , Técnicas Imunoenzimáticas , Testes Imunológicos/métodos , Lipoproteínas/análise , Lipoproteínas/genética , Doença de Lyme/imunologia , Neuroborreliose de Lyme , Exame FísicoRESUMO
BACKGROUND: The population dynamics of vector-borne pathogens inside the arthropod vector can have important consequences for vector-to-host transmission. Tick-borne spirochete bacteria of the Borrelia burgdorferi (sensu lato) species complex cause Lyme borreliosis in humans and spend long periods of time (>12 months) in their Ixodes tick vectors. To date, few studies have investigated the dynamics of Borrelia spirochete populations in unfed Ixodes nymphal ticks. METHODS: Larval ticks from our laboratory colony of I. ricinus were experimentally infected with B. afzelii, and killed at 1 month and 4 months after the larva-to-nymph moult. The spirochete load was also compared between engorged larval ticks and unfed nymphs (from the same cohort) and between unfed nymphs and unfed adult ticks (from the same cohort). The spirochete load of B. afzelii in each tick was estimated using qPCR. RESULTS: The mean spirochete load in the 1-month-old nymphs (~14,000 spirochetes) was seven times higher than the 4-month-old nymphs (~2000 spirochetes). Thus, the nymphal spirochete load declined by 80% over a period of 3 months. An engorged larval tick acquired ~100 spirochetes, and this population was 20 times larger in a young, unfed nymph. The spirochete load also appeared to decline in adult ticks. Comparison between wild and laboratory populations found that lab ticks were more susceptible to acquiring B. afzelii. CONCLUSION: The spirochete load of B. afzelii declines dramatically over time in domesticated I. ricinus nymphs under laboratory conditions. Future studies should investigate whether temporal declines in spirochete load occur in wild Ixodes ticks under natural conditions and whether these declines influence the tick-to-host transmission of Borrelia.
Assuntos
Vetores Artrópodes/microbiologia , Grupo Borrelia Burgdorferi/isolamento & purificação , Ixodes/microbiologia , Doença de Lyme/transmissão , Animais , Vetores Artrópodes/fisiologia , Grupo Borrelia Burgdorferi/genética , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Humanos , Ixodes/crescimento & desenvolvimento , Ixodes/fisiologia , Larva/crescimento & desenvolvimento , Larva/microbiologia , Doença de Lyme/microbiologia , Ninfa/crescimento & desenvolvimento , Ninfa/microbiologia , Spirochaetales/genética , Spirochaetales/crescimento & desenvolvimento , Spirochaetales/isolamento & purificação , Fatores de TempoRESUMO
The growth of 29 human strains from the three main pathogenic species of Borrelia burgdorferi sensu lato on a solid BSK-based medium was compared in two culture atmospheres: 3% CO(2) air and anaerobiosis. All strains grew under anaerobic conditions, whereas only 13 strains were able to grow in aerobiosis with 3% CO(2) (P<0.001). In the latter condition, 75% of the B. burgdorferi sensu stricto strains grew versus 33% of the B. garinii and B. afzelii strains. These data suggest that, especially for B. garinii and B. afzelii species, anaerobic conditions enhance growth yield and speed of low-passage Borrelia strains.
Assuntos
Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Anaerobiose , Técnicas Bacteriológicas , Técnicas de Cultura de Células , Meios de Cultura , Viabilidade MicrobianaRESUMO
The results of a longitudinal epidemiological survey in two contrasting habitats in an area of the Mazury Lakes district of Poland indicate that both host and vector (Ixodes ricinus) densities, may be the most important risk factors for the tick-transmitted spirochetes of Borrelia burgdirferi s.l. However, the results also highlight that even related host species, such as the wild rodents Apodemus flavicollis and Clethrionomys glareolus that share the same habitat, can show quite different dynamics of tick infestation. We provide evidence that the woodland populations of A. flavicollis and C. glareolus are more frequently infested with larvae than nymphs, and more frequently with both stages than M. arvalis in the neighbouring open fallow lands. The prevalence of infestation with larvae varied from 92 % for A. flavicollis, and 76 % for C. glareolus to 37 % for M. arvalis. Other factors, such as population age structure and sex, were also shown to impact on tick densities on hosts at particular times of the year and hence on the zoonotic risk. Moreover, particular species of rodents from different habitats, A. flavicollis (woodlands) and Microtus arvalis (fallow lands) carry infected immature I. ricinus ticks more frequently than C. glareolus voles (woodlands). Thus, the relative contribution of each species to the cumulative reservoir competence differs among species living in the woodland habitats and in relation to voles living in the fallow lands. It follows, therefore, that any factor which reduces the relative density of A. flavicollis in comparison to other hosts in the wild rodent community, will reduce also the risk of human exposure to Lyme borreliosis spirochetes.
Assuntos
Vetores Artrópodes/microbiologia , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Ixodes/microbiologia , Doença de Lyme/veterinária , Doenças dos Roedores/epidemiologia , Infestações por Carrapato/epidemiologia , Animais , DNA Bacteriano/isolamento & purificação , Reservatórios de Doenças , Ecossistema , Monitoramento Ambiental , Monitoramento Epidemiológico , Interações Hospedeiro-Parasita , Humanos , Larva , Estudos Longitudinais , Doença de Lyme/epidemiologia , Doença de Lyme/parasitologia , Polônia/epidemiologia , Doenças dos Roedores/parasitologia , Estações do Ano , Infestações por Carrapato/parasitologia , Infestações por Carrapato/veterináriaRESUMO
Lyme borreliosis is caused by the spirochete Borrelia burgdorferi sensu lato, a fastidious bacterium that replicates slowly and requires special conditions to grow in the laboratory. Borrelia isolation from clinical material is a golden standard for microbiological diagnosis of borrelial infection. Important factors that affect in vitro borrelia growth are temperature of incubation and number of borrelia cells in the sample. The aim of the study was to assess the influence of temperature on borrelia growth and survival by evaluation and comparison of growth of 31 different borrelia strains at five different temperatures and to determine the influence of different inoculums on borrelia growth at different temperatures. Borreliae were cultured in the MKP medium; the initial and final number of spirochetes was determined by dark field microscopy using Neubauer counting chamber. The growth of borrelia was defined as final number of cells/mL after three days of incubation. For all three Borrelia species, the best growth was found at 33°C, followed by 37, 28, and 23°C, while no growth was detected at 4°C (P<0.05). The growth of B. afzelii species was weaker in comparison to the other two species at 23, 28, 33 and 37°C (P<0.05), respectively. There was no statistically significant difference between the growth of B. garinii and B. burgdorferi sensu stricto at 28, 33, and 37°C (P>0.05), respectively. Inoculum had statistically significant influence on growth of all three Borrelia species at all tested temperatures except at 4°C.
Assuntos
Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Borrelia burgdorferi/crescimento & desenvolvimento , Carga Bacteriana , Borrelia burgdorferi/genética , Borrelia burgdorferi/isolamento & purificação , Grupo Borrelia Burgdorferi/genética , Grupo Borrelia Burgdorferi/isolamento & purificação , Meios de Cultura/química , Humanos , Doença de Lyme/microbiologia , Tipagem de Sequências Multilocus , Especificidade da Espécie , TemperaturaRESUMO
Studies of the protein function of Borrelia burgdorferi have been limited by a lack of tools for manipulating borrelial DNA. We devised a system to study the function of a B. burgdorferi oligopeptide permease (Opp) orthologue by complementation with Escherichia coli Opp proteins. The Opp system of E. coli has been extensively studied and has well defined substrate specificities. The system is of interest in B. burgdorferi because analysis of its genome has revealed little identifiable machinery for synthesis or transport of amino acids and only a single intact peptide transporter operon. As such, peptide uptake may play a major role in nutrition for the organism. Substrate specificity for ABC peptide transporters in other organisms is determined by their substrate binding protein. The B. burgdorferi Opp operon differs from the E. coli Opp operon in that it has three separate substrate binding proteins, OppA-1, -2 and -3. In addition, B. burgdorferi has two OppA orthologues, OppA-4 and -5, encoded on separate plasmids. The substrate binding proteins interact with integral membrane proteins, OppB and OppC, to transport peptides into the cell. The process is driven by two ATP binding proteins, OppD and OppF. Using opp-deleted E. coli mutants, we transformed cells with B. burgdorferi oppA-1, -2, -4 or -5 and E. coli oppBCDF. All of the B. burgdorferi OppA proteins are able to complement E. coli OppBCDF to form a functional Opp transport system capable of transporting peptides for nutritional use. Although there is overlap in substrate specificities, the substrate specificities for B. burgdorferi OppAs are not identical to that of E. coli OppA. Transport of toxic peptides by B. burgdorferi grown in nutrient-rich medium parallels borrelial OppA substrate specificity in the complementation system. Use of this complementation system will pave the way for more detailed studies of B. burgdorferi peptide transport than currently available tools for manipulating borrelial DNA will allow.
Assuntos
Proteínas de Bactérias , Grupo Borrelia Burgdorferi/enzimologia , Escherichia coli/enzimologia , Escherichia coli/genética , Proteínas de Membrana Transportadoras/metabolismo , Sequência de Aminoácidos , Transporte Biológico Ativo , Grupo Borrelia Burgdorferi/genética , Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Escherichia coli/crescimento & desenvolvimento , Deleção de Genes , Genes Bacterianos , Teste de Complementação Genética , Proteínas de Membrana Transportadoras/genética , Oligopeptídeos/química , Oligopeptídeos/metabolismo , Óperon , Plasmídeos/genéticaRESUMO
The impact of Ixodes ricinus salivary gland extract (SGE) on inflammatory changes in the skin and draining lymph nodes of mice, elicited by the infection with the important human pathogen, B. afzelii, was determined using flow cytometry. SGE injected together with spirochetes reduced the numbers of leukocytes and gammadelta-T lymphocytes in infected epidermis at early time-points post infection. In draining lymph nodes, the anti-inflammatory effect of SGE was manifested by the decrease of total cell count compared with that in mice treated with inactivated SGE. Changes in subpopulations of immunocompetent cells apparently reflected the effect of SGE on the proliferation of spirochetes in the host. The significance of tick saliva anti-inflammatory effect for saliva activated transmission of B. afzelii is shown.
Assuntos
Grupo Borrelia Burgdorferi/crescimento & desenvolvimento , Doença de Lyme/imunologia , Linfonodos/imunologia , Pele/imunologia , Carrapatos/imunologia , Animais , Feminino , Doença de Lyme/patologia , Doença de Lyme/transmissão , Linfonodos/patologia , Contagem de Linfócitos , Subpopulações de Linfócitos , Camundongos , Camundongos Endogâmicos C57BL , Glândulas Salivares/imunologia , Pele/patologia , Extratos de Tecidos/imunologiaRESUMO
Low (7th) and high (298th/304th) in vitro passages (cultivated over a period of 3 years) of two human Borrelia burgdorferi sensu lato strains, PKo (B. afzelii) and PBi (B. garinii) were compared by pulse-field gel electrophoresis, Southern blot, sequencing of the ospA gene, SDS-PAGE and Western blot. Digestion of genomic DNA with ApaI, BssHII, KspI, MluI, SmaI and XhoI did not reveal any differences between low and high passages. The loss of two linear plasmids with sizes of 6 and 31 kbp was detected in strain PKo between passages 34-50 and 101-304, respectively, whereas the ospA-carrying plasmid remained unchanged. In contrast, analysis of linear plasmid profiles obtained from low and high passages of B. garinii strain PBi showed no differences. Sequence analysis of the ospA gene demonstrated no difference in the strain PBi and one nucleotide exchange in the strain PKo when low and high passages were compared. The observed transition (G-A) in the third codon position did not alter the amino acid sequence. However, the rate of expression of the outer surface proteins OspA, OspB and OspC of strain PKo during low and high stages of cultivation varied significantly. In summary, our data suggest that the B. burgdorferi sensu lato genome is stable during long-term in vitro cultivation.