RESUMO
BACKGROUND: Bavaria, a large federal state in Germany, has been declared free from infections with Bovine Alphaherpesvirus 1 (BoHV-1) in 2011. To maintain this status the cattle population is monitored for antibodies against BoHV-1 regularly. Several years ago, infrequent but recurrent problems in this sero-surveillance were statistically put into correlation with the presence of antibodies against Bovine Alphaherpesvirus 2 (BoHV-2). In Europe, BoHV-2 is primarily known as the agent causing bovine herpes mammillitis. However, very little information about BoHV-2 infections in Bavaria is available so far. Therefore, the aims of this study were to determine BoHV-2 seroprevalences and to detect virus genomes in potential clinical samples. RESULTS: 6801 blood sera of healthy cattle from all over Bavaria were tested for antibodies against BoHV-2, revealing an overall seroprevalence of 5.51%. Interestingly, seroprevalences markedly varied between the North and the South of Bavaria, namely from 0.42 to 11.17%. Concurrently, the previously reported relation between the epidemiologically inexplicable sero-reactivities in BoHV-1 ELISAs and the presence of BoHV-2 infections were statistically corroborated in this study. To detect BoHV-2 genomes a fast and sensitive real time PCR was established. Using a multiple PCR strategy, tissue samples from skin lesions at relevant localizations, corresponding lymph nodes, and trigeminal ganglia from 111 animals, as well as nasal swabs from 918 bovines with respiratory symptoms were tested. However, BoHV-2 genomes were not detected in any of these samples. CONCLUSIONS: BoHV-2 antibodies were found in samples from bovines all over Bavaria, albeit with an explicit South-North-divide. BoHV-2 genomes, however, could not be detected in any of the analyzed samples, indicating that acute clinical cases as well as obvious virus reactivation are relatively rare. Consequently, the future spread of BoHV-2 infections throughout Bavaria, particularly, after eradicating BoHV-1, has to be further monitored.
Assuntos
Doenças dos Bovinos/virologia , Infecções por Herpesviridae/veterinária , Herpesvirus Bovino 2/isolamento & purificação , Animais , Anticorpos Antivirais/análise , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/imunologia , Ensaio de Imunoadsorção Enzimática/veterinária , Alemanha , Infecções por Herpesviridae/epidemiologia , Infecções por Herpesviridae/imunologia , Herpesvirus Bovino 2/genética , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Estudos SoroepidemiológicosRESUMO
BACKGROUND: Bovine alphaherpesvirus type 2 (BoHV-2) belongs to family Herpesviridae, subfamily Alphaherpesviridae and can cause two distinct, well-defined conditions: a generalized benign skin infection that somewhat mimics lumpy skin disease (LSD), referred to as Pseudo-Lumpy Skin Disease (PSLD) and a localized ulcerative mammillitis, referred to as Bovine Herpetic Mammillitis (BHM). BHM is a localized form of BoHV-2 infection that causes erosive-ulcerative self-limiting lesions on breast and nipples. BHM is chiefly a disease of lactating dairy cows and has been described sporadically in several countries. In this study we describe an outbreak of bovine herpetic mammillitis caused by BoHV-2 occurred in a dairy farm in Southern Italy. Clinical signs were observed in 26/59 lactating cows with the age ranging between 2 and 6 years. The affected animals were afebrile, showed lesions on the skin of nipples, breast and ventral surface of the abdomen, near the mammary veins and spontaneously recovered within 2 months. RESULTS: BoHV-2 DNA was detected in the crust samples by pan-herpes PCR and real-time quantitative PCR. The virus was isolated on bovine kidney cells and was characterised by deep sequencing technologies. The nucleotide identity to BoHV-2 of the strain ITA/2018/468 retrieved in this study ranged from 98.83 to 100%. Phylogenetic analyses based on three full-length gene (glycoprotein B, thymidine kinase and glycoprotein G) sequences confirmed the close relatedness of the strain ITA/2018/468 to BoHV-2 sequences. CONCLUSIONS: The report represents a significant outbreak of BHM in a dairy farm 50 years after the last description in Italy. However, outbreaks of PLSD have been described in Europe recently, indicating that the virus is present in European territories. Improving the diagnostic algorithms and enacting specific surveillance plans could be useful to understand better the epidemiological and pathogenetic patterns of BoHV-2 infection in livestock animals, and to develop, eventually, effective prophylaxis plans.
Assuntos
Doenças dos Bovinos/virologia , Infecções por Herpesviridae/veterinária , Herpesvirus Bovino 2/isolamento & purificação , Glândulas Mamárias Animais/virologia , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , DNA Viral/isolamento & purificação , Indústria de Laticínios , Surtos de Doenças/veterinária , Feminino , Infecções por Herpesviridae/epidemiologia , Herpesvirus Bovino 2/classificação , Herpesvirus Bovino 2/genética , Itália/epidemiologia , Lactação , Filogenia , Análise de Sequência de DNARESUMO
Bovine herpetic mammillits is a self-limiting cutaneous disease of the udder and teats of cows associated with bovine herpesvirus 2 (BoHV-2) whose pathogenesis is poorly understood. This article describes the use of guinea pigs (Cavia porcellus) to study the pathogenesis of BoHV-2 infection. Twelve weanling female guinea pigs inoculated subcutaneously with BoHV-2 in the genitalia and teats developed local hyperemia, edema, vesicles, ulcers and scabs. Infectious virus was recovered between days 3 and 7 post-infection (pi) from the genital area (9/12) and teats (1/12); and all inoculated animals seroconverted (virus-neutralizing titers of 16-128). Histological examination of lesions revealed lymphoplasmacytic perivascular infiltrates and intranuclear inclusion bodies in keratinocytes. PCR examination of tissues collected at day 35 pi detected latent viral DNA predominantly in lumbosacral spinal segments. In another experiment, eight females inoculated with BoHV-2 in the genitalia and treated with dexamethasone (Dx) at day 35 pi developed mild to moderate local signs, yet no virus could be recovered from lesions. PCR examination of spinal segments from these animals confirmed the presence of latent viral DNA. These results demonstrate that guinea pigs are susceptible to BoHV-2 infection and therefore may be used to study selected aspects of BoHV-2 biology.
Assuntos
Modelos Animais de Doenças , Cobaias , Herpes Simples/veterinária , Herpesvirus Bovino 2 , Latência Viral , Doença Aguda , Animais , Anticorpos Antivirais/sangue , Bovinos , Doenças dos Bovinos/virologia , DNA Viral/análise , Dexametasona/farmacologia , Feminino , Genitália Feminina/virologia , Herpes Simples/patologia , Herpes Simples/virologia , Herpesvirus Bovino 2/genética , Herpesvirus Bovino 2/imunologia , Herpesvirus Bovino 2/isolamento & purificação , Herpesvirus Bovino 2/fisiologia , Glândulas Mamárias Animais/virologia , Testes de Neutralização , Reação em Cadeia da Polimerase , Medula Espinal/virologia , Proteínas do Envelope Viral/imunologia , Ativação Viral , Replicação ViralRESUMO
A semiquantitative evaluation of potential bacterial pathogens was correlated to the severity of lesions during an outbreak of bovine necrotic vulvovaginitis (BNVV) on an Israeli dairy herd. Bacteriologic examination of 287 vaginal swabs from 104 post-calving heifers showed a highly significant correlation between Porphyromonas levii colony forming unit numbers and the clinical scores of the lesions, when assessed by an ordinal regression statistical model. No such correlation was found for the other bacteria included in the study. Nineteen samples taken for virological examinations resulted negative for bovine herpes viruses 1, 2, 4 and 5. Thus the results of this study substantiate the essential role of P. levii in the etiology of BNVV and indicate that BHV4 is not required as a predisposing factor to the syndrome.
Assuntos
Doenças dos Bovinos/microbiologia , Surtos de Doenças/veterinária , Vulvovaginite/veterinária , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Feminino , Herpesvirus Bovino 1/isolamento & purificação , Herpesvirus Bovino 2/isolamento & purificação , Herpesvirus Bovino 4/isolamento & purificação , Herpesvirus Bovino 5/isolamento & purificação , Necrose/microbiologia , Porphyromonas/isolamento & purificação , Vulvovaginite/epidemiologia , Vulvovaginite/microbiologiaRESUMO
We describe 3 outbreaks of superficial dermatitis caused by bovine herpesvirus 2 (BoHV-2) in dairy breed calves. Clinically, all of the affected calves were 12-26 d of age, had alopecia and crusts on the face and ears, and were non-pruritic and afebrile. Affected animals recovered spontaneously without any treatment within 2-4 wk after onset of clinical signs based on 1 herd with follow up. Histologic examination of all skin crust or tissue samples identified neutrophilic inflammation, mild hyperkeratosis, multinucleate syncytial cells, and intranuclear inclusion bodies in the syncytial cells. Real-time PCR testing on affected surface crusts or tissue provided evidence of BoHV-2, and testing, where performed, was negative for parapoxvirus including bovine papular stomatitis virus and the ovine form of malignant catarrhal fever tested in EDTA blood samples. Bovine viral diarrhea virus also was negative by ELISA, as well as bovine herpesvirus 1 by immunohistochemistry. Direct electron microscopy of infected tissues in the first outbreak revealed herpesvirus-like particles.
Assuntos
Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/epidemiologia , Surtos de Doenças/veterinária , Herpes Simples/veterinária , Herpesvirus Bovino 2/isolamento & purificação , Animais , California/epidemiologia , Bovinos , Doenças dos Bovinos/virologia , Orelha/patologia , Feminino , Cabeça/patologia , Herpes Simples/diagnóstico , Herpes Simples/epidemiologia , Herpes Simples/virologia , Pele/patologiaRESUMO
Polymerase chain reaction (PCR) was used to test six different nonbovine ruminant species for five bovine herpesviruses including infectious bovine rhinotracheitis virus (BoHV-1), bovine herpes mammillitis virus (BoHV-2), Movar-type herpesvirus (BoHV-4), bovine herpesvirus type 5 (BoHV-5), and alcelaphine herpesvirus 1 (AlHV-1). Species tested included 56 roe deer (Capreolus capreolus), 66 red deer (Cervus elaphus), 20 fallow deer (Dama dama), 16 mouflon (Ovis musimon), 34 domestic sheep, and 44 domestic goats, which were sampled in Hungary in 2003. Tracheal and popliteal lymph nodes collected from these animals were tested for the presence of the five bovine herpesviruses using three nested (two of which were duplex) PCR assays. Three bovine herpesviruses (BoHV-1, -2, and -4) were detected, whereas no evidence of AlHV-1 or BoHV-5 was observed. Prevalence of BoHV-1 ranged from 12% to 47%, and PCR-positive results were observed in all species tested. BoHV-2 was detected from roe deer, red deer, fallow deer, mouflon, and domestic sheep, and the prevalence in these species ranged from 3% to 50%. BoHV-4 was detected in all species, with prevalence ranging from 12% to 69%. Sequenced PCR products were 99-100% identical to bovine herpesviral sequences deposited in the GenBank.
Assuntos
Cervos/virologia , Doenças das Cabras/epidemiologia , Infecções por Herpesviridae/veterinária , Herpesviridae/isolamento & purificação , Doenças dos Ovinos/epidemiologia , Animais , Animais Selvagens/virologia , Sequência de Bases , Cabras , Infecções por Herpesviridae/epidemiologia , Herpesvirus Bovino 1/isolamento & purificação , Herpesvirus Bovino 2/isolamento & purificação , Herpesvirus Bovino 4/isolamento & purificação , Herpesvirus Bovino 5/isolamento & purificação , Hungria/epidemiologia , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/veterinária , Sensibilidade e Especificidade , Alinhamento de Sequência/veterinária , Ovinos , Especificidade da EspécieRESUMO
Bridge Technology is an amplification technique in which pairs of primers are immobilized on a solid support, allowing amplification only at the location of the primer pair spot. The technique has diagnostic potential since an array of primer pairs, each specific for a different pathogen, can be used with a diagnostic sample without inter-pair interactions that plague the development of multiplex PCRs. As a result, one assay should be able to determine which of multiple pathogens are present and which are absent in each sample. As test material, we examined the specificity of detection of the RNA-containing bovine viral diarrhea virus (BVDV) and two DNA-containing bovine herpesviruses 1 and 2 (BHV-1 and BHV-2). Nylon membranes with two spots of UV-immobilized primer pairs--one for BVDV and one for BHV--were used in amplification with both corresponding templates, with each template singly and with no template. When amplification was assayed by chemiluminescent detection of incorporated DIG-nucleotides, the expected amplification patterns were obtained.
Assuntos
Vírus da Diarreia Viral Bovina Tipo 1/isolamento & purificação , Vírus da Diarreia Viral Bovina Tipo 2/isolamento & purificação , Herpesvirus Bovino 1/isolamento & purificação , Herpesvirus Bovino 2/isolamento & purificação , Membranas Artificiais , Nylons , Reação em Cadeia da Polimerase/métodos , Animais , Sequência de Bases , Bovinos , Linhagem Celular , Primers do DNA , DNA Complementar , DNA Viral/análise , Vírus da Diarreia Viral Bovina Tipo 1/genética , Vírus da Diarreia Viral Bovina Tipo 2/genética , Herpesvirus Bovino 1/genética , Herpesvirus Bovino 2/genéticaRESUMO
Bovine herpesvirus 1 (BHV-1) strains can be differentiated by their DNA and polypeptide patterns, and by antigenic properties as demonstrated by monoclonal antibodies. We classified the BHV-1 strains according to these data as BHV-1.1, BHV-1.2 (a/b) and BHV-1.3 (a/b). BHV-1.1 and BHV-1.2 correspond to the well known 'common' BHV-1 strains, whereas BHV-1.3 has only recently been recognized and exhibits a neuropathogenic potential. In the present paper we describe the structural genome characteristics of BHV-1.3 compared to those of the other BHV-1 strains, examined by means of restriction site mapping, electron microscopy and cross-hybridization. Our results also confirm and complete data concerning BHV-1.1 and BHV-1.2 published by other authors. The following main conclusions can be drawn from our investigations: BHV-1.1 and BHV-1.2 differences are restricted to distinct genomic regions, characterized by loss or gain of restriction sites. BHV-1.3, however, differs from the other BHV-1 strains in restriction site alterations throughout the whole genome. Electron microscopy showed the typical BHV-1 DNA structure for BHV-1.3. Genetic homology between BHV-1.1 and BHV-1.2, reported to be about 95%, was confirmed by cross-hybridization, and a similar high base sequence homology for BHV-1.3 could be shown.
Assuntos
Doenças dos Bovinos/microbiologia , Genes Virais , Herpesviridae/genética , Herpesvirus Bovino 2/genética , Doenças do Sistema Nervoso/veterinária , Animais , Bovinos , Enzimas de Restrição do DNA , DNA Viral/ultraestrutura , Herpesvirus Bovino 2/isolamento & purificação , Herpesvirus Bovino 2/patogenicidade , Microscopia Eletrônica , Doenças do Sistema Nervoso/microbiologia , Hibridização de Ácido Nucleico , Biossíntese de ProteínasRESUMO
Bovid herpesvirus 2 infection was studied in calves exposed to the virus by intradermal inoculation of the skin of the left cheek or by nasal spray. In either case a localised infection developed and virus replication was shown to occur mostly in the tissues of its primary localisation, i.e. the skin of the left cheek or the nasal mucosa. There were neither secondary lesions, except at the site of virus injection, nor any serious systemic involvement on the part of the animals. The virus was also recovered from some areas of the skin (right cheek, perineum and scrotum) that were free of macroscopic lesions; moreover, intranuclear inclusions were found in several tissues of the nervous system (brain, superior cervical, stellate and Gasserian ganglia) which did not show any signs of inflammatory or degenerative changes. These findings suggest that the skin and the nervous system play an important role in the naturally-occurring disease since they could be the sites where the virus is maintained latently in the host.
Assuntos
Doenças dos Bovinos/etiologia , Infecções por Herpesviridae/veterinária , Administração Intranasal , Animais , Bovinos , Doenças dos Bovinos/microbiologia , Doenças dos Bovinos/patologia , Infecções por Herpesviridae/etiologia , Infecções por Herpesviridae/microbiologia , Herpesvirus Bovino 2/isolamento & purificação , Herpesvirus Bovino 2/fisiologia , Injeções Intradérmicas , Masculino , Replicação ViralRESUMO
Three different polymerase chain reaction (PCR) protocols were evaluated for their ability to detect bovine herpesvirus 2 (BoHV-2): single-step PCR with 3 reaction stages (denaturation, annealing and extension), 2 reaction stages (denaturation and annealing/extension; shuttle PCR), and semi-nested PCR with 3 reaction stages. All the PCR protocols showed the same sensitivity (detection limit of 0.4 TCID(50)). A non-specific band sometimes appeared in mock cell DNA at annealing temperatures below 64 degrees C. The shuttle PCR was found to be superior to the other protocols under consideration because of the speed of its application. Furthermore, no non-specific band was detected in DNAs of eight other DNA viruses. Thus, the shuttle PCR seems to be an excellent diagnostic tool for BoHV-2 infections.
Assuntos
Infecções por Herpesviridae/diagnóstico , Herpesvirus Bovino 2/genética , Herpesvirus Bovino 2/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Animais , Bovinos , Doenças dos Bovinos/diagnóstico , DNA Viral/análise , DNA Viral/genética , Infecções por Herpesviridae/veterinária , Camundongos , Camundongos Endogâmicos BALB C , Sensibilidade e EspecificidadeRESUMO
The behavior of Bovid herpesvirus 2 in the skin, lymph nodes, and nervous system of calves injected intravenously with the virus was studied. After a latent phase that lasted up to 40 hours after inoculation, the virus produced a systemic infection which was characterized by primary localization of virus in the skin, lymph nodes, and nervous system. Between postinoculation days 7 and 12, the infection tended to persist in the skin only. The finding of intranuclear inclusions in the pyramidal cells of the cerebral cortex and in the neurons of the superior cervical and stellate ganglia supports the hypothesis that the virus might utilize the structures of the nervous system for its replicative cycle in the calf.
Assuntos
Doenças dos Bovinos/microbiologia , Infecções por Herpesviridae/veterinária , Animais , Bovinos , Doenças dos Bovinos/patologia , Infecções por Herpesviridae/microbiologia , Infecções por Herpesviridae/patologia , Herpesvirus Bovino 2/isolamento & purificação , Injeções Intravenosas , Linfonodos/microbiologia , Masculino , Pele/microbiologia , Pele/patologiaRESUMO
The relationship of 3 dermatotropic bovine herpes-viruses (bovine mamillitis herpesvirus, dermatotropic bovine herpesvirus, and Allerton virus) was investigated; special emphasis was on the clinical response and the development of complement-fixation and precipitating antibodies in cattle. The immune respnse in steers was determined throughout the disease and after challenge exposure of the steers with 1 of the other 2 herpesviruses. Blood samples and sections of skin were collected intermittently from infected animals and examined for presence of virus.
Assuntos
Doenças dos Bovinos , Infecções por Herpesviridae/veterinária , Dermatopatias Infecciosas/veterinária , Animais , Anticorpos Antivirais/análise , Bovinos , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/microbiologia , Reações Cruzadas , Febre/veterinária , Herpesviridae/imunologia , Herpesviridae/isolamento & purificação , Infecções por Herpesviridae/imunologia , Infecções por Herpesviridae/microbiologia , Herpesvirus Bovino 2/imunologia , Herpesvirus Bovino 2/isolamento & purificação , Masculino , Pele/microbiologiaRESUMO
Bovine herpes mammillitis was diagnosed in 2 New York dairy herds. Lesions ranged from vesiculation and ulceration of large (up to 10 cm wide) areas of udder and teat skin to single small (2-3 cm wide) plaques of edema. Some lesions resembled "umbilicated pocks" characteristic of cowpox virus infections. Recently freshened heifers were the most severely affected; older cows and heifers with less turgid udders had milder lesions. In 2 cows, incurable mastitis developed. In other cows, the lesions healed by centripetal growth of epidermis into the lesions. Diagnosis was made by isolation of bovid herpesvirus 2 from lesions in both herds and by serum-neutralization testing. Virus isolated from a cow in 1 herd was injected into 9 members of the same herd and may have been responsible for the absence of lesions on these animals; all other members of the herd were affected.
Assuntos
Doenças dos Bovinos/diagnóstico , Surtos de Doenças/veterinária , Infecções por Herpesviridae/veterinária , Animais , Anticorpos Antivirais/análise , Bovinos , Doenças dos Bovinos/imunologia , Diagnóstico Diferencial , Feminino , Infecções por Herpesviridae/diagnóstico , Infecções por Herpesviridae/imunologia , Herpesvirus Bovino 2/imunologia , Herpesvirus Bovino 2/isolamento & purificação , Glândulas Mamárias Animais/microbiologia , Testes de Neutralização , New YorkRESUMO
Beef cattle from a herd in north Alabama were examined because of an outbreak of nonfatal skin disease characterized by discrete circumscribed areas of inflammation that developed on the skin from the neck to the hips. Areas of inflammation, which tended to be superficial, underwent necrosis and scabbed over. The scabs eventually dropped off leaving discrete, round, whitish, hairless lesions that were 1.2 to 2.5 cm diameter. Because clinical signs were consistent with those expected with pseudo-lumpy skin disease (PLSD) caused by bovine herpesvirus type 2 (BHV-2), samples from 16 representative animals were submitted for BHV-2 testing. All 16 animals were seropositive for BHV-2, but the virus could not be isolated from skin biopsy specimens or buffy coat samples. Results of a polymerase chain reaction assay incorporating primers designed to amplify 2 DNA sequences from BHV-2 were positive for 3 of the 10 cattle, suggesting that skin lesions in these cattle were a result of PLSD. Our findings suggest that PLSD may be more common and widespread in the United States than suggested by the frequency with which BHV-2 has been isolated from cattle with PLSD-like skin lesions.
Assuntos
Doenças dos Bovinos/diagnóstico , DNA Viral/análise , Herpes Simples/veterinária , Herpesvirus Bovino 2/isolamento & purificação , Reação em Cadeia da Polimerase/veterinária , Alabama/epidemiologia , Animais , Sequência de Bases , Bovinos , Doenças dos Bovinos/epidemiologia , Surtos de Doenças/veterinária , Feminino , Herpes Simples/diagnóstico , Herpes Simples/epidemiologia , Herpesvirus Bovino 2/genética , Doença Nodular Cutânea/diagnóstico , Masculino , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/métodos , Pele/patologiaRESUMO
A region of the UL24 gene of six Australian field isolates of Bovine herpesvirus 2 (BHV-2) was sequenced after a passage in Madin-Darby bovine kidney (MDBK) cells by polymerase chain reaction (PCR). While the PCR product covered the first half of the UL24 gene, a particular interest was focused on the 274-297 nucleotide (nt) region in which a two nt deletion had previously been detected in the BHM-1 strain of BHV-2. Most isolates tested did not generate any defective UL24 genes during the passage. However, a third of the UL24 genes of BHM-1 strain contained the two nts deletion, but only when a high multiplicity of infection (MOI) was used. Also in the isolate 554 at least a half of the UL24 genes were found to be altered independently of the MOT used. These UL24 genes had an insertion of four nts within the 274-297 nt region. The predicted truncation of the UL24 protein of both viruses occurred at the same stop codon. The region of the gene in which these mutations of the UL24 gene occurred is common to all herpesviruses.
Assuntos
Herpesvirus Bovino 2/genética , Proteínas Virais/genética , Sequência de Aminoácidos , Animais , Austrália , Sequência de Bases , Bovinos , Linhagem Celular , Códon/genética , Herpes Simples/virologia , Herpesvirus Bovino 2/isolamento & purificação , Dados de Sequência Molecular , Mutação , Reação em Cadeia da Polimerase , Alinhamento de SequênciaRESUMO
Ten viruses isolated from swabs and vesicular fluid collected from the teats of dairy cattle on 4 properties in Northern Victoria were identified as bovine herpes mammillitis (BHM) viruses by their physico-chemical and morphological properties and serological relationship to each other and a Scottish Strain of BHM virus. The viruses, isolated in bovine kidney and testicular cell cultures, produced cytopathic effects characterised by very large syncytia and eosinophilic intranuclear inculsion bodies. The intradermal inoculation of BHM virus into two cattle produced necrosis and ulceration of the skin of the teats about the area of inoculation and the development of serum neutralising antibody. After healing of the ulcers on day 37 after inoculation, the cattle were intravenously inoculated with corticosteroid for 6 days but BHM virus was not re-isolated from the teat skin or vaginal or nasal swabs.
Assuntos
Doenças dos Bovinos/microbiologia , Herpesviridae/isolamento & purificação , Herpesvirus Bovino 2/isolamento & purificação , Glândulas Mamárias Animais , Viroses/veterinária , Animais , Anticorpos Antivirais/análise , Antígenos Virais , Bovinos , Doenças dos Bovinos/etiologia , Feminino , Herpesvirus Bovino 2/imunologia , Herpesvirus Bovino 2/patogenicidade , Viroses/etiologia , Viroses/microbiologiaRESUMO
Bovine herpes mammillitis was diagnosed in a dairy herd with udder and teat skin lesions. Clinical symptoms seen in 6 cows consisted of round dry areas at the teats as well as large red and painful areas with crust formation at the teats, the teat basis and the udder. Diagnosis was verified by demonstrating numerous virus particles with the typical herpes structure and by BHV-2 serum neutralization test. Prevalence of BHV-2 in the herd was determined by using BHV-2 SNT at 7 occasions during a period of 15 months. The relatively low BHV-2 SNT-titres as well as the seasonal increase of BHV-2 titres and seroprevalence in the month of September were indicative of a chronic and latent BHV-2 infection in the herd.
Assuntos
Doenças dos Bovinos/diagnóstico , Herpes Simples/veterinária , Herpesvirus Bovino 2 , Glândulas Mamárias Animais , Animais , Anticorpos Antivirais/sangue , Bovinos , Doenças dos Bovinos/virologia , Feminino , Herpes Simples/diagnóstico , Herpes Simples/virologia , Herpesvirus Bovino 2/imunologia , Herpesvirus Bovino 2/isolamento & purificação , Masculino , Glândulas Mamárias Animais/patologia , Glândulas Mamárias Animais/virologiaRESUMO
An outbreak of sheep associated malignant catarrhal fever in crossbred cattle in a village of Andhra Pradesh, southern India, affected thirteen adult cows and two calves from a population of forty animals. All the affected animals were died between December and January 2013-14. The clinical and gross postmortem findings were typical of MCF in Indian crossbred cattle. Migrating sheep flocks were suspected source of infection for the cattle. The diagnosis was confirmed by heminested PCR in all the affected cattle and the suspected sheep flock. The PCR provided evidence of ovine herpes virus type 2.