Ottonia martiana Miq. (
Piperaceae),
planta conhecida popularmente por "
anestésia" e empregada no
tratamento de
odontalgias devido à sua ação anestésica sobre a
mucosa oral, foi investigada por meio de ensaios
antibacterianos de
difusão em disco de
papel e de bioautografia frente a microorganismos
presentes na
microbiota oral humana [
Streptococcus mutans (ATCC 25175),
Streptococcus mitis (ATCC 49456),
Streptococcus pyogenes (ATCC 19615),
Streptococcus salivarius (ATCC 25975),
Escherichia coli (ATCC 11229 e 25922),
Pseudomonas aeruginosa (ATCC 27853),
Enterobacter aerogenes (ATCC 27853). Os resultados dos
bioensaios mostraram que o
extrato bruto de O. martiana (32.9 mg mL-1) apresenta potencial
antibacteriano frente às
bactérias Gram-positivas testadas. Dentre as substâncias bioativas detectadas foram identificadas a piperovatina (Rf 0.35), piperlonguminina (Rf 0.52) e a isopiperlonguminina (Rf 0.52). A piperovatina e isopiperlonguminina foram isoladadas do extrato das raízes de O. martiana, guiadas pelo teste de bioautografia.
Ottonia martiana Miq. (
Piperaceae), a
plant known popularly in southern
Brazil as "anestésia" and used in the
treatment of
odontalgia for its
anesthetic action on the
oral mucosa, was investigated for antibacterial activity by
paper disc
agar diffusion and bioautographic
methods, against microorganisms present in the
human oral cavity [
Streptococcus mutans (ATCC 25175),
Streptococcus mitis (ATCC 49456),
Streptococcus pyogenes (ATCC 19615),
Streptococcus salivarius (ATCC 25975),
Escherichia coli (ATCC 11229 and 25922),
Pseudomonas aeruginosa (ATCC 27853) and
Enterobacter aerogenes (ATCC 27853).The
crude extract of O. martiana (32.9 mg mL-1) had antibacterial potential against all
Gram-positive bacteria tested.
Analysis of the bioautograms led to the
detection of bioactive substances, among which it was possible to identify piperovatine (Rf 0.35), piperlonguminine (Rf 0.52) and isopiperlonguminine (Rf 0.52). The piperovatine and isopiperlonguminine were isolated from the roots of O. martiana, guided by a bioautographic antibacterial
bioassay.