Effects of lipopolysaccharide on production of interleukin-1 and interleukin-6 by bovine mammary epithelial cells in vitro.
J Vet Med Sci
; 61(1): 33-5, 1999 Jan.
Article
em En
| MEDLINE
| ID: mdl-10027160
ABSTRACT
This investigation was performed to determine the effect of lipopolysaccharide (LPS) on production of interleukin (IL)-1 and IL-6 by bovine mammary epithelial cells in vitro. After confluence, the cells were stimulated with LPS (0.1, 1.0 or 10 micrograms/ml) for 4, 8, 24, and 48 hr. LPS increased production of both IL-1 and IL-6 production from mammary cells in a dose dependent manner. The expression of mRNA for IL-1 receptor antagonist (IL-1ra) was demonstrated by reverse transcription-polymerase chain reaction in bovine mammary epithelial cells.
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Base de dados:
MEDLINE
Assunto principal:
Lipopolissacarídeos
/
Interleucina-6
/
Interleucina-1
/
Glândulas Mamárias Animais
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article