Crocalbin: a new calcium-binding protein that is also a binding protein for crotoxin, a neurotoxic phospholipase A2.
FEBS Lett
; 445(2-3): 440-4, 1999 Feb 26.
Article
em En
| MEDLINE
| ID: mdl-10094503
ABSTRACT
Utilizing Marathon-ready cDNA library and a gene-specific primer corresponding to a partial amino acid sequence determined previously, the complete nucleotide sequence for the cDNA of crocalbin, which binds crotoxin (a phospholipase A2) and Ca2+, was obtained by polymerase chain reaction. The open reading frame of the cDNA encodes a novel polypeptide of 315 amino acid residues, including a signal sequence of 19 residues. This protein contains six potential Ca(2+)-binding domains, one N-glycosylation site, and a large amount of acidic amino acid residues. The ability to bind Ca2+ has been ascertained by calcium overlay experiment. Evidenced by sequence similarity in addition, it is concluded that crocalbin is a new member of the reticulocalbin family of calcium-binding proteins.
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Base de dados:
MEDLINE
Assunto principal:
Fosfolipases A
/
Proteínas de Ligação ao Cálcio
/
Crotoxina
/
Neurotoxinas
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article