Telomere shortening triggers a p53-dependent cell cycle arrest via accumulation of G-rich single stranded DNA fragments.
Oncogene
; 18(37): 5148-58, 1999 Sep 16.
Article
em En
| MEDLINE
| ID: mdl-10498864
ABSTRACT
It has been repeatedly suspected that telomere shortening might be one possible trigger of the p53-dependent cell cycle arrest, although the mechanism of this arrest remained unclear. Telomeres in human cells under mild oxidative stress accumulate single-strand damage faster than interstitial repetitive sequences. In MRC-5 fibroblasts and U87 glioblastoma cells, which both express wild-type p53, oxidative stress-mediated production of single-strand damage in telomeres is concomitant to the accumulation of p53 and p21 and to cell cycle arrest. This response can be modeled by treatment of cells with short single stranded telomeric G-rich DNA fragments. The arrest is transient in U87 cells. Recovery from it is accompanied by up-regulation of telomerase activity and elongation of telomeres. Overexpression of mutated p53 is sufficient to reverse the phenotype of inhibition as well as the delayed activation of telomerase. These data suggest that the production of G-rich single stranded fragments during the course of telomere shortening is sufficient to trigger a p53 dependent cell cycle arrest.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
DNA de Cadeia Simples
/
Ciclo Celular
/
Proteína Supressora de Tumor p53
/
Telômero
/
Fragmentação do DNA
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article