Ethanol has differential effects on rat neuron and thymocyte reactive oxygen species levels and cell viability.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol
; 124(1): 83-9, 1999 Sep.
Article
em En
| MEDLINE
| ID: mdl-10579652
ABSTRACT
In rat thymocytes and cerebellar granule cells, reactive oxygen species (ROS) levels were increased and cell viability was decreased as a result of exposure to ethanol (up to 0.4%). Thymocytes showed larger increases in ROS levels, but neurons showed more pronounced decreases in cell viability. These parameters in neurons were relatively unaffected when the cells were incubated with ethanol in the presence of inhibitors of alcohol-oxidizing enzymes, but in thymocytes, the presence of diallyl sulfide (an inhibitor of alcohol-inducible cytochrome P450, CYP2E1) or 4-methylpyrazole (an inhibitor of CYP2E1 and alcohol dehydrogenase) caused decreases in ROS production from ethanol. In both cell types, the presence of 3-aminotriazole (an inhibitor of catalase) did not decrease ROS production from ethanol. These studies show that the cytotoxic effects of ethanol in neurons may not be the result of oxidative metabolism of ethanol, whereas in thymocytes, the cytotoxic effect of ethanol is principally a result of its oxidative metabolism.
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Base de dados:
MEDLINE
Assunto principal:
Timo
/
Espécies Reativas de Oxigênio
/
Etanol
/
Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article