Molecular cloning and characterization of maize Toc34, a regulatory component of the protein import machinery of chloroplast.
Biochim Biophys Acta
; 1491(1-3): 309-14, 2000 Apr 25.
Article
em En
| MEDLINE
| ID: mdl-10760596
ABSTRACT
Several components of the chloroplast protein import machinery have been identified mostly in the C3 dicot plant pea. We describe here two homologous cDNA sequences of Toc34, a regulatory component of the import machinery, from maize, a C4 monocot plant. The deduced amino acid sequences of two maize Toc34 proteins, named ZmToc34-1 and ZmToc34-2, show 95% identity to each other and about 60% to those of previously identified Toc34 proteins. The GTP-binding motif and the presumed carboxyl terminal membrane anchor are also conserved in the ZmToc34 proteins. The maize Toc34, like that of pea, is tightly associated with the outer envelope membrane of chloroplast and exposed to the cytosol.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Cloroplastos
/
Zea mays
/
Proteínas de Membrana
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article