Expression and intracellular localization of mouse Vasa-homologue protein during germ cell development.
Mech Dev
; 93(1-2): 139-49, 2000 May.
Article
em En
| MEDLINE
| ID: mdl-10781947
ABSTRACT
To demonstrate the cellular and subcellular localization of mouse vasa homologue protein during germ cell development, specific antibody was raised against the full-length MVH protein. The immunohistochemical analyses demonstrated that MVH protein was exclusively expressed in primordial germ cells just after their colonization of embryonic gonads and in germ cells undergoing gametogenic processes until the post-meiotic stage in both males and females. The co-culture of EG cells with gonadal somatic cells indicated inductive MVH expression caused by an intercellular interaction with gonadal somatic cells. In adult testis, MVH protein was localized in the cytoplasm of spermatogenic cells, including chromatoid bodies in spermatids, known to be a perinuclear nuage structure which includes polar granules that contain VASA protein in Drosophila.
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Base de dados:
MEDLINE
Assunto principal:
Oogênese
/
Espermatogênese
/
RNA Helicases
Limite:
Animals
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article