A mechanism for coupling exit from mitosis to partitioning of the nucleus.
Cell
; 102(1): 21-31, 2000 Jul 07.
Article
em En
| MEDLINE
| ID: mdl-10929710
ABSTRACT
Exit from mitosis must not occur prior to partitioning of chromosomes between daughter cells. We find that the GTP binding protein Tem1, a regulator of mitotic exit, is present on the spindle pole body that migrates into the bud during S phase and mitosis. Tem1's exchange factor, Lte1, localizes to the bud. Thus, Tem1 and Lte1 are present in the same cellular compartment (the bud) only after the nucleus enters the bud during nuclear division. We also find that the presence of Tem1 and Lte1 in the bud is required for mitotic exit. Our results suggest that the spatial segregation of Tem1 and Lte1 ensures that exit from mitosis only occurs after the genetic material is partitioned between mother and daughter cell.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Fúngicas
/
Núcleo Celular
/
Proteínas Tirosina Fosfatases
/
Proteínas Monoméricas de Ligação ao GTP
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Fatores de Troca do Nucleotídeo Guanina
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Proteínas de Saccharomyces cerevisiae
/
Mitose
/
Fuso Acromático
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article