Induction of anti-DNA antibodies in preautoimmune NZBxNZW F1 mice by immunization with a DNA-DNase I complex.
J Autoimmun
; 15(1): 9-13, 2000 Aug.
Article
em En
| MEDLINE
| ID: mdl-10936023
ABSTRACT
Recent studies suggest that anti-DNA antibodies may arise from the immune response to a complex of DNA and a DNA-binding protein. One of the protein targets frequently recognized by anti-DNA antibodies is the enzyme DNase I. To investigate the possible role of DNase I in the induction of anti-DNA antibodies, we immunized preautoimmune NZBxNZW F1 mice with a complex of DNA and DNase I emulsified in complete Freund's adjuvant. Control mice received DNA or DNase in adjuvant. IgG anti-dsDNA antibodies were induced in 50% of the mice immunized with DNA-DNase, in 25% of the mice immunized with DNase and in 6% of the mice immunized with DNA. However, immunized mice that produced anti-DNA antibodies did not develop renal disease. These data show that a DNA-binding protein like DNase may act as carrier in the immune response that leads to anti-DNA antibodies production in an autoimmune strain, but the induced anti-dsDNA antibodies have a low pathogenic potential.
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Base de dados:
MEDLINE
Assunto principal:
Doenças Autoimunes
/
DNA
/
Anticorpos Antinucleares
/
Desoxirribonuclease I
Tipo de estudo:
Etiology_studies
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article