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Recognition of preproteins by the isolated TOM complex of mitochondria.
Stan, T; Ahting, U; Dembowski, M; Künkele, K P; Nussberger, S; Neupert, W; Rapaport, D.
Afiliação
  • Stan T; Institut für Physiologische Chemie der Universität München, Goethestrabetae 33, D-80336 München, Germany.
EMBO J ; 19(18): 4895-902, 2000 Sep 15.
Article em En | MEDLINE | ID: mdl-10990453
A multisubunit complex in the mitochondrial outer membrane, the TOM complex, mediates targeting and membrane translocation of nuclear-encoded preproteins. We have isolated the TOM holo complex, containing the preprotein receptor components Tom70 and Tom20, and the TOM core complex, which lacks these receptors. The interaction of recombinant mitochondrial preproteins with both types of soluble TOM complex was analyzed. Preproteins bound efficiently in a specific manner to the isolated complexes in the absence of chaperones and lipids in a bilayer structure. Using fluorescence correlation spectroscopy, a dissociation constant in the nanomolar range was determined. The affinity was lower when the preprotein was stabilized in its folded conformation. Following the initial binding, the presequence was transferred into the translocation pore in a step that required unfolding of the mature part of the preprotein. This translocation step was also mediated by protease-treated TOM holo complex, which contains almost exclusively Tom40. Thus, the TOM core complex, consisting of Tom40, Tom22, Tom6 and Tom7, is a molecular machine that can recognize and partially translocate mitochondrial precursor proteins.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Membrana Transportadoras / Precursores de Proteínas / Receptores Citoplasmáticos e Nucleares / Receptores de Superfície Celular / Proteínas de Saccharomyces cerevisiae / Mitocôndrias Idioma: En Ano de publicação: 2000 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Membrana Transportadoras / Precursores de Proteínas / Receptores Citoplasmáticos e Nucleares / Receptores de Superfície Celular / Proteínas de Saccharomyces cerevisiae / Mitocôndrias Idioma: En Ano de publicação: 2000 Tipo de documento: Article