More keys to padlock probes: mechanisms for high-throughput nucleic acid analysis.
Curr Opin Biotechnol
; 12(1): 11-5, 2001 Feb.
Article
em En
| MEDLINE
| ID: mdl-11167066
ABSTRACT
With the impending availability of total information about nucleic acid sequences in humans and other organisms, tools to investigate these sequences on a large scale assume increasing importance. Methods currently in use, however, cannot offer the required combination of high-throughput, sensitivity and specificity of detection. Padlock probes, circularizing oligonucleotides, may provide a means to detect, distinguish, quantitate and also locate very large numbers of DNA or RNA sequences. Recent developments in areas such as the biochemistry of ligation and characterization of ligases, methods to replicate circularized probes and the development of assays based on these principles augment the potential of padlock probes.
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Base de dados:
MEDLINE
Assunto principal:
Sondas de Oligonucleotídeos
/
Técnicas de Sonda Molecular
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article