Metabolic significance and expression of Caenorhabditis elegans type II 3-oxoacyl-CoA thiolase.
Cell Biochem Biophys
; 32 Spring: 291-3, 2000.
Article
em En
| MEDLINE
| ID: mdl-11330061
ABSTRACT
The authors cloned the cDNA of the nematode Caenorhabditis elegans encoding a 44-kDa protein (P-44), which is similar to sterol carrier protein x (SCPx). Genomic DNA data and Northern blot analysis excluded the possibility of P-44 forming SCPx-like fusion protein. P-44 is required in the formation of bile acid in vitro from CoA esters of their enoyl-form intermediate in the presence of D-3-hydroxyacyl-CoA dehydratase/D-3-dehydrogenase bifunctional protein. Also, rat SCPx converts 24-hydroxy-form intermediate to bile acid under similar conditions. From this and other evidence, P-44 and SCPx were categorized as type II thiolase. The mRNA encoding P-44 was detected in every developmental stage of C. elegans egg, larval stages, and adult. P-44, therefore, seems essential for the normal functioning of this organism.
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Base de dados:
MEDLINE
Assunto principal:
Acetil-CoA C-Aciltransferase
/
Caenorhabditis elegans
Limite:
Animals
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article