Laser raman spectroscopy--new probe of myosin substructure.
Science
; 188(4191): 933-6, 1975 May 30.
Article
em En
| MEDLINE
| ID: mdl-1138362
ABSTRACT
Laser Raman spectroscopy is used to probe the heterogeneous substructure of the large contractile protein myosin. Some peaks are assigned to specific chemical groups of the molecule; others, notably the conformationally sensitive amide III vibrations, provide information on the structurally distinct regions of the molecule. Deuteration of the NH groups is instrumental in the assignment of these vibrational modes. The relative intensities of bands typical of alpha-helical conformations (near 1265 and 1304 cm-1) and bands associated with nonhelical structure (near 1244 cm-1) are sensitive indicators of myosin substructure and represent potentially useful probes of conformational changes.
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Base de dados:
MEDLINE
Assunto principal:
Espalhamento de Radiação
/
Análise Espectral
/
Miosinas
/
Lasers
Limite:
Animals
Idioma:
En
Ano de publicação:
1975
Tipo de documento:
Article