A method that allows easy characterization of tumor-infiltrating lymphocytes.
J Immunol Methods
; 253(1-2): 163-75, 2001 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-11384678
ABSTRACT
A method was developed to compare the lymphocytic infiltrates in regressing vs. progressing experimental mouse tumors using a model for human papillomavirus-16 (HPV-16) oncoprotein-linked cancer. Tumor cells mixed with matrigel, composed of natural matrix substances that provide a basement membrane structure for adherent cells, were inoculated into mice vaccinated with an efficacious vaccine to the E7 oncoprotein or a vaccine to a control antigen. The tumor cells remained within the solidified gel and recruited a cellular infiltrate that could readily be analyzed upon removal of the gelatinous mass containing progressing or regressing tumors. The results show that tumors recruit activated CD8(+) T cells regardless of their antigen specificity. In regressing tumors expressing an appropriate target antigen for the vaccine-induced CD8(+) T cells, a strong increase of the tumor antigen-specific T cell population was observed over time. Progressing tumors that lacked the target antigen for the activated CD8(+) T cell population did not show this selective enrichment.
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Base de dados:
MEDLINE
Assunto principal:
Proteoglicanas
/
Infecções Tumorais por Vírus
/
Linfócitos do Interstício Tumoral
/
Colágeno
/
Laminina
/
Linfócitos T CD8-Positivos
/
Infecções por Papillomavirus
/
Combinação de Medicamentos
/
Neoplasias Experimentais
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article