Characterisation of VanA and VanB elements from glycopeptide-resistant Enterococcus faecium from Greece.
J Med Microbiol
; 50(8): 682-687, 2001 Aug.
Article
em En
| MEDLINE
| ID: mdl-11478671
ABSTRACT
Ten glycopeptide-resistant Enterococcus faecium isolates from separate patients in Laikon General Hospital, Athens were studied. Eight isolates had the VanA phenotype and represented variants of three strains based on SmaI macrorestriction banding patterns. Their VanA elements were compared with the prototype element, Tn1546, by an overlapping PCR method. Three related isolates contained resistance elements indistinguishable from Tn1546 (designated Greek type I). The other five isolates all contained identical elements that differed from Tn1546 by the presence of IS1251 between vanS and vanH, by a point mutation (G --> T) at nucleotide position 8234 within vanX and by a partial loss of transposition gene orf1 (designated Greek type II). Two distinct strains of E. faecium with the VanB phenotype were obtained. HhaI digestion of an amplified fragment of the vanB gene indicated that both strains contained the vanB2 allele, and further PCR assays confirmed that the vanB2 gene cluster was located within a Tn5382-like element.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Enterococcus faecium
/
Carbono-Oxigênio Ligases
/
Antibacterianos
Limite:
Humans
País/Região como assunto:
Europa
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article