Sulindac induces apoptosis, inhibits proliferation and activates caspase-3 in Hep G2 cells.
Anticancer Res
; 22(1A): 263-6, 2002.
Article
em En
| MEDLINE
| ID: mdl-12017300
ABSTRACT
BACKGROUND:
It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated hepatoma cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated hepatoma cells and what its potential mechanism might be. MATERIALS ANDMETHODS:
Hep G2 cells were treated with sulindac at different concentrations. Apoptosis rate, cell proliferation and 3H-thymidine incorporation were measured. The activities of caspase-3, acid and neutral sphingomyelinase and the changes of sphingomyelin content were also assayed.RESULTS:
Sulindac dose-dependently induced apoptosis in Hep G2 cells; both sulindac sulfone and sulfide had similar effects. The apoptosis was accompanied by an increase of caspase-3 activity and a decrease of cell proliferation and 3H-thymidine incorporation. No significant change could be observed for the activity of sphingomyelinase and sphingomyelin content.CONCLUSION:
Sulindac induces apoptosis and inhibits proliferation in Hep G2 cells. The effect may be mediated by a pathway related to caspase-3 activation but independent of sphingomyelin metabolism
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Base de dados:
MEDLINE
Assunto principal:
Sulindaco
/
Apoptose
/
Carcinoma Hepatocelular
/
Caspases
/
Neoplasias Hepáticas
/
Antineoplásicos
Limite:
Humans
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article